Geldanamycin, a heat shock protein 90-binding benzoquinone ansamycin, inhibits steroid-dependent translocation of the glucocorticoid receptor from the cytoplasm to the nucleus.

Abstract:

:When they are translated, steroid receptors are assembled into a multiprotein complex containing hsp90, p23, an immunophilin, and often some hsp70. Some of the receptors, such as that for progesterone, have nuclear localization signals that are functional in the absence of hormone, and they move into the nucleus where they exist in the same multiprotein heterocomplex with hsp90. Other receptors, such as the glucocorticoid receptor, are localized predominantly in the cytoplasm in the absence of hormone and move into the nucleus in a hormone-dependent fashion. We have previously proposed that hsp90 and the immunophilin play a role in receptor trafficking [Pratt, W. B. (1993) J. Biol. Chem. 268, 21455-21458]. In this work, we show that treatment of L cells with geldanamycin, a benzoquinone ansamycin that binds to hsp90 and disrupts its function, impedes dexamethasone-dependent trafficking of the glucocorticoid receptor from the cytoplasm to the nucleus. Because geldanamycin treatment of hormone-free cells causes a rapid loss of steroid binding activity, receptors were prebound with dexamethasone by incubating cells with hormone at 0 degrees C prior to shifting the temperature to 37 degrees C for 20 min to permit receptor transformation and translocation in the presence or absence of geldanamycin. Geldanamycin does not cause steroid to dissociate from prebound receptors, and it does not inhibit hormone-mediated receptor transformation assayed by conversion to the DNA-binding state. However, as reported previously for the progesterone receptor, geldanamycin blocks assembly of the glucocorticoid receptor-hsp90 heterocomplex at an intermediate state of assembly where the receptor is bound to hsp70 and p60, both of which are required components in the assembly mechanism. Our observations support the proposal that dynamic association of receptors with hsp90 is required for receptor translocation from the cytoplasm to the nucleus.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Czar MJ,Galigniana MD,Silverstein AM,Pratt WB

doi

10.1021/bi970648x

subject

Has Abstract

pub_date

1997-06-24 00:00:00

pages

7776-85

issue

25

eissn

0006-2960

issn

1520-4995

pii

bi970648x

journal_volume

36

pub_type

杂志文章
  • Polyketide Ring Expansion Mediated by a Thioesterase, Chain Elongation and Cyclization Domain, in Azinomycin Biosynthesis: Characterization of AziB and AziG.

    abstract::The azinomycins are a family of potent antitumor agents with the ability to form interstrand cross-links with DNA. This study reports on the unusual biosynthetic formation of the 5-methyl naphthoate moiety, which is essential for effective DNA association. While sequence analysis predicts that the polyketide synthase ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.5b01050

    authors: Mori S,Simkhada D,Zhang H,Erb MS,Zhang Y,Williams H,Fedoseyenko D,Russell WK,Kim D,Fleer N,Ealick SE,Watanabe CM

    更新日期:2016-02-02 00:00:00

  • Contrasting synergistic anion effects in vanadium(V) binding to nicatransferrin versus human serum transferrin.

    abstract::Some ascidians sequester vanadium and other metal ions that are bound and transported in higher organisms by transferrin. The ascidian Ciona intestinalis has a monolobal transferrin (nicatransferrin) in its plasma. The binding of vanadium(V) to nicatransferrin was investigated by using isothermal titration calorimetry...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi901630j

    authors: Gaffney JP,Valentine AM

    更新日期:2009-12-15 00:00:00

  • Thermodynamic parameters of the binding of retinol to binding proteins and to membranes.

    abstract::Retinol (vitamin A alcohol) is a hydrophobic compound and distributes in vivo mainly between binding proteins and cellular membranes. To better clarify the nature of the interactions of retinol with these phases which have a high affinity for it, the thermodynamic parameters of these interactions were studied. The tem...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00468a014

    authors: Noy N,Xu ZJ

    更新日期:1990-04-24 00:00:00

  • Thermodynamic and kinetic basis of interfacial activation: resolution of binding and allosteric effects on pancreatic phospholipase A2 at zwitterionic interfaces.

    abstract::A general kinetic model for catalysis by interfacial enzymes is developed. It couples the Michaelis-Menten catalytic turnover cycle at the interface with that in the aqueous phase through the distribution equilibria between the interface and the surrounding aqueous phase. Analysis under two limiting conditions fully d...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi970855x

    authors: Berg OG,Rogers J,Yu BZ,Yao J,Romsted LS,Jain MK

    更新日期:1997-11-25 00:00:00

  • Molecular dynamics simulations of pentapeptides at interfaces: salt bridge and cation-pi interactions.

    abstract::Peptide-membrane interactions are important for understanding the binding, partitioning, and folding of membrane proteins; the activity of antimicrobial and fusion peptides; and a number of other processes. We describe molecular dynamics simulations (10-25 ns) of two pentapeptides Ace-WLXLL (with X = Arg or Lys side c...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi027001j

    authors: Aliste MP,MacCallum JL,Tieleman DP

    更新日期:2003-08-05 00:00:00

  • The catalytic mechanism of kynureninase from Pseudomonas fluorescens: evidence for transient quinonoid and ketimine intermediates from rapid-scanning stopped-flow spectrophotometry.

    abstract::The reaction of Pseudomonas fluorescens kynureninase with L-kynurenine and L-alanine has been examined using rapid-scanning stopped-flow spectrophotometry. Mixing kynureninase with 0.5 mM L-kynurenine results in formation of a quinonoid intermediate, with lambdamax = 494 nm, within the dead time (ca. 2 ms) of the stop...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi980066v

    authors: Phillips RS,Sundararaju B,Koushik SV

    更新日期:1998-06-16 00:00:00

  • Monomeric sarcosine oxidase: evidence for an ionizable group in the E.S complex.

    abstract::Monomeric sarcosine oxidase (MSOX) contains covalently bound FAD and catalyzes the oxidation of sarcosine (N-methylglycine) and other secondary amino acids, including L-proline. The reductive half-reaction with L-proline proceeds via a rapidly attained equilibrium (K(d)) between free E(ox) and the E(ox).S complex, fol...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi020285n

    authors: Zhao G,Jorns MS

    更新日期:2002-08-06 00:00:00

  • Nucleotide sequence and organization of the human S-protein gene: repeating peptide motifs in the "pexin" family and a model for their evolution.

    abstract::The S-protein/vitronectin gene was isolated from a human genomic DNA library, and its sequence of about 5.3 kilobases including the adjacent 5' and 3' flanking regions was established. Alignment of the genomic DNA nucleotide sequence and the cDNA sequence indicated that the gene consisted of eight exons and seven intr...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00395a024

    authors: Jenne D,Stanley KK

    更新日期:1987-10-20 00:00:00

  • An unnatural folate stereoisomer is catalytically competent in DNA photolyase.

    abstract::The folate chromophore in native Escherichia coli DNA photolyase ([6R]-5,10-CH+-H4Pte(Glu)n=3-6) serves as an antenna, transferring light energy to the fully reduced flavin (FADH2) reaction center at high efficiency (EET = 0.92). Apophotolyase reconstituted after an overnight incubation with [6R,S]-5,10-CH+-H4folate (...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9605548

    authors: Lipman RS,Jorns MS

    更新日期:1996-06-18 00:00:00

  • A protein arginine N-methyltransferase 1 (PRMT1) and 2 heteromeric interaction increases PRMT1 enzymatic activity.

    abstract::Protein arginine N-methyltransferases (PRMTs) act in signaling pathways and gene expression by methylating arginine residues within target proteins. PRMT1 is responsible for most cellular arginine methylation activity and can work independently or in collaboration with other PRMTs. In this study, we demonstrate a dire...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi200644c

    authors: Pak ML,Lakowski TM,Thomas D,Vhuiyan MI,Hüsecken K,Frankel A

    更新日期:2011-09-27 00:00:00

  • Restoration of the activity of active-site mutants of the hyperthermophilic beta-glycosidase from Sulfolobus solfataricus: dependence of the mechanism on the action of external nucleophiles.

    abstract::The beta-glycosidase from the hyperthermophilic Archaeon Sulfolobus solfataricus hydrolyzes beta-glycosides following a retaining mechanism based upon the action of two amino acids: Glu387, which acts as the nucleophile of the reaction, and Glu206, which acts as the general acid/base catalyst. The activities of inacti...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi981855f

    authors: Moracci M,Trincone A,Perugino G,Ciaramella M,Rossi M

    更新日期:1998-12-08 00:00:00

  • Comparative orientation of the fluorene residue in native DNA modified by N-acetoxy-N-2-acetylaminofluorene and two 7-halogeno derivatives.

    abstract::Native calf thymus DNA was reacted with N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) and its 7-fluoro and 7-iodo derivatives. Different ways of purification of the fluorene modified DNA samples were checked in order ot obtain a nucleic acid free from all noncovalently bound fluorene residues. The decrease in melting ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00660a027

    authors: Fuchs RP,Lefevre JF,Pouyet J,Daune MP

    更新日期:1976-07-27 00:00:00

  • Helix variants of troponin C with tailored calcium affinities.

    abstract::Muscle fiber contraction is regulated through calcium-induced changes in the conformation of troponin C. In this study, we explored the relationship between the stability of a specific helix in the protein and the metal ion affinity of associated binding sites. Serial replacement of the amino acid at position 130 caus...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00088a038

    authors: Trigo-Gonzalez G,Awang G,Racher K,Neden K,Borgford T

    更新日期:1993-09-21 00:00:00

  • Binding efficiency of protein-protein complexes.

    abstract::We examine the relationship between binding affinity and interface size for reversible protein-protein interactions (PPIs), using cytokines from the tumor necrosis factor (TNF) superfamily and their receptors as a test case. Using surface plasmon resonance, we measured single-site binding affinities for binding of the...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi301039t

    authors: Day ES,Cote SM,Whitty A

    更新日期:2012-11-13 00:00:00

  • Estimation of the distance change between cysteine-457 and the nucleotide binding site when sodium pump changes conformation from E1 to E2 by fluorescence energy transfer measurements.

    abstract::The first indication of the size of a conformational change implicated in ion transport by sodium pump has been obtained by measuring the change in efficiency of fluorescence energy transfer between two specific locations on the alpha-subunit. The donor (5'-(iodoacetamido)fluorescein) attaches covalently to cysteine-4...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi960407+

    authors: Lin SH,Faller LD

    更新日期:1996-06-25 00:00:00

  • Multivector fluorescence analysis of the xpt guanine riboswitch aptamer domain and the conformational role of guanine.

    abstract::Purine riboswitches are RNA regulatory elements that control purine metabolism in response to intracellular concentrations of the purine ligands. Conformational changes of the guanine riboswitch aptamer domain induced by guanine binding lead to transcriptional regulation of genes involved in guanine biosynthesis. The ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9019912

    authors: Brenner MD,Scanlan MS,Nahas MK,Ha T,Silverman SK

    更新日期:2010-03-02 00:00:00

  • Tryptophan residues flanking the second transmembrane helix (TM2) set the signaling state of the Tar chemoreceptor.

    abstract::The chemoreceptors of Escherichia coli are homodimeric membrane proteins that cluster in patches near the cell poles. They convert environmental stimuli into intracellular signals that control flagellar rotation. The functional domains of a receptor are physically separated by the cell membrane. Chemoeffectors bind to...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi048969d

    authors: Draheim RR,Bormans AF,Lai RZ,Manson MD

    更新日期:2005-02-01 00:00:00

  • Disrupted zinc-binding sites in structures of pathogenic SOD1 variants D124V and H80R.

    abstract::Mutations in human copper-zinc superoxide dismutase (SOD1) cause an inherited form of the fatal neurodegenerative disease amyotrophic lateral sclerosis (ALS). Here, we present structures of the pathogenic SOD1 variants D124V and H80R, both of which demonstrate compromised zinc-binding sites. The disruption of the zinc...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi100314n

    authors: Seetharaman SV,Winkler DD,Taylor AB,Cao X,Whitson LJ,Doucette PA,Valentine JS,Schirf V,Demeler B,Carroll MC,Culotta VC,Hart PJ

    更新日期:2010-07-13 00:00:00

  • Essential sulfhydryl for reduced nicotinamide adenine dinucleotide binding in D-beta-hydroxybutyrate dehydrogenase.

    abstract::Chemical derivatization studies have been directed at the sulfhydryl group of D-beta-hydroxybutyrate dehydrogenase, a lipid-requiring enzyme. Reaction with N-ethylmaleimide leads to progressive and parallel loss of both enzymic activity and coenzyme binding. Both functions are lost when 1 equiv of sulfhydryl is deriva...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00564a021

    authors: Latruffe N,Brenner SC,Fleischer S

    更新日期:1980-11-11 00:00:00

  • Redox cycles of vitamin E: hydrolysis and ascorbic acid dependent reduction of 8a-(alkyldioxy)tocopherones.

    abstract::Oxidation of the biological antioxidant alpha-tocopherol (vitamin E; TH) by peroxyl radicals yields 8a-(alkyldioxy)tocopherones, which either may hydrolyze to alpha-tocopheryl quinone (TQ) or may be reduced by ascorbic acid to regenerate TH. To define the chemistry of this putative two-electron TH redox cycle, we stud...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00451a034

    authors: Liebler DC,Kaysen KL,Kennedy TA

    更新日期:1989-12-12 00:00:00

  • 1H NMR study of the base-pairing reactions of d(GGAATTCC): salt and polyamine effects on the imino proton exchange.

    abstract::Salts and polyamines have a variety of effects on the physical properties of DNA, including stabilization against thermal melting. We wished to gain greater insight into the mechanism of this stabilization by ascertaining its effect on the dynamics of base opening and closing reactions, as measured by NMR. Since the b...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00404a018

    authors: Braunlin WH,Bloomfield VA

    更新日期:1988-02-23 00:00:00

  • Location of major antigenic sites of the beta subunit of human chorionic gonadotropin.

    abstract::Purified beta subunit of human chorionic gonadotropin (hCG) was partially reduced with beta-mercaptoethanol, carboxymethylated, and digested with chymotrypsin. The peptides were isolated by high-voltage electrophoresis and paper chromatography. Five major disulfide-containing peptides were isolated, and their location...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00619a034

    authors: Swaminathan N,Braunstein GD

    更新日期:1978-12-26 00:00:00

  • Dimorphism in bile salt/lecithin mixed micelles.

    abstract::We investigated the dependence of certain properties of bile salt/lecithin mixed micelles on the molar ratio of the two components. UV absorption suggested that the micellar system is fundamentally altered on passing through a bile salt:lecithin molar ratio of 1.8:1. Differential scanning calorimetry confirmed this tr...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00505a029

    authors: Claffey WJ,Holzbach RT

    更新日期:1981-01-20 00:00:00

  • 31P NMR saturation-transfer measurements in Saccharomyces cerevisiae: characterization of phosphate exchange reactions by iodoacetate and antimycin A inhibition.

    abstract::31P nuclear magnetic resonance (NMR) saturation-transfer (ST) techniques have been used to measure steady-state flows through phosphate-adenosine 5'-triphosphate (ATP) exchange reactions in glucose-grown derepressed yeast. Our results have revealed that the reactions catalyzed by glyceraldehyde-3-phosphate dehydrogena...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00397a043

    authors: Campbell-Burk SL,Jones KA,Shulman RG

    更新日期:1987-11-17 00:00:00

  • Two-dimensional NMR studies of staphylococcal nuclease. 1. Sequence-specific assignments of hydrogen-1 signals and solution structure of the nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+ ternary complex.

    abstract::Staphylococcal nuclease H124L is a recombinant protein produced in Escherichia coli whose sequence is identical with that of the nuclease produced by the V8 variant of Staphylococcus aureus. The enzyme-metal ion activator-nucleotide inhibitor ternary complex, nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+, was inves...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00453a011

    authors: Wang JF,LeMaster DM,Markley JL

    更新日期:1990-01-09 00:00:00

  • Protein engineering of subtilisin BPN': enhanced stabilization through the introduction of two cysteines to form a disulfide bond.

    abstract::Introduction of a disulfide bond by site-directed mutagenesis was found to enhance the stability of subtilisin BPN' (EC 3.4.21.14) under a variety of conditions. The location of the new disulfide bond was selected with the aid of a computer program, which scored various sites according to the amount of distortion that...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00382a002

    authors: Pantoliano MW,Ladner RC,Bryan PN,Rollence ML,Wood JF,Poulos TL

    更新日期:1987-04-21 00:00:00

  • Crystal structure of a prolonged-acting insulin with albumin-binding properties.

    abstract::The fatty acid acylated insulin, Lys(B29)-tetradecanoyl, des-(B30) human insulin, has been crystallized and the structure determined by X-ray crystallography. The fatty acid substituent on residue B29 Lys binds reversibly to circulating albumin protein in vivo, and by this mechanism the hormone's action is prolonged. ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9625105

    authors: Whittingham JL,Havelund S,Jonassen I

    更新日期:1997-03-11 00:00:00

  • Human ferrochelatase is an iron-sulfur protein.

    abstract::Recombinant human ferrochelatase has been expressed in Escherichia coli and purified to homogeneity. Metal analyses revealed approximately 2 mol of non-heme Fe per mol of the purified enzyme (M(r) = 40,000). The UV-visible absorption spectrum of the purified enzyme consists of a protein absorption at 278 nm (epsilon a...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00168a003

    authors: Dailey HA,Finnegan MG,Johnson MK

    更新日期:1994-01-18 00:00:00

  • Nucleotide sequence of the promoter and fadB gene of the fadBA operon and primary structure of the multifunctional fatty acid oxidation protein from Escherichia coli.

    abstract::The primary structure of a multifunctional protein, the large alpha-subunit of the Escherichia coli fatty acid oxidation complex, was determined by sequencing the fadB region of the fadBA operon. The amino-terminal sequence of this protein had been established by Edman degradation. The transcription start site of the ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00241a023

    authors: Yang XY,Schulz H,Elzinga M,Yang SY

    更新日期:1991-07-09 00:00:00

  • Reversible phosphorylation as a controlling factor for sustaining calcium oscillations in HeLa cells: Involvement of calmodulin-dependent kinase II and a calyculin A-inhibitable phosphatase.

    abstract::The role of reversible phosphorylation in histamine-induced Ca2+ oscillations in HeLa cells has been investigated by using various activators and inhibitors of protein kinases and phosphatases. Electroporation was employed to introduce impermeable materials into single cells, which proved to be a useful and convenient...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi952471h

    authors: Zhu DM,Tekle E,Chock PB,Huang CY

    更新日期:1996-06-04 00:00:00