Abstract:
:Sequential 1H NMR assignments of a metallothionein from Neurospora crassa have been accomplished by the combined use of COSY, 2QF-COSY, HOHAHA, and rotating-frame NOE experiments. All potentially observable resonances were assigned except for the epsilon-NH3 group of the C-terminal lysine. 1H NOEs, when observed in the laboratory frame and at 500-MHz spectrometer frequency, were negligible in this protein due to the inherent rotational correlation time of the molecule. This difficulty was circumvented by measuring transverse NOEs in the rotating frame under spin-locking conditions. The observed pattern of NOEs reveals a marked absence of "regular" secondary structures in the protein. Thus, the stability of this metallothionein's tertiary structure must arise primarily from its metal ligation. This appears to be a general feature of MTs since a general lack of extensive secondary structural elements was also observed in other metallothioneins.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Malikayil JA,Lerch K,Armitage IMdoi
10.1021/bi00433a038subject
Has Abstractpub_date
1989-04-04 00:00:00pages
2991-5issue
7eissn
0006-2960issn
1520-4995journal_volume
28pub_type
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