Abstract：
:Protein kinase C-related kinases (PRKs) are serine/threonine kinases that are members of the protein kinase C superfamily and can be activated by binding to members of the Rho family of small G proteins via a Rho binding motif known as an HR1 domain. The PRKs contain three tandem HR1 domains at their N-termini. The structure of the HR1a domain from PRK1 in complex with RhoA [Maesaki, R., et al. (1999) Mol. Cell 4, 793-803] identified two potential contact interfaces between the G protein and the HR1a domain. In this work, we have used an alanine scanning mutagenesis approach to identify whether both contact sites are used when the two proteins interact in solution and also whether HR1b, the second HR1 domain from PRK1, plays a role in binding to RhoA. The mutagenesis identified just one contact site as being relevant for binding of RhoA and HR1a in solution, and the HR1b domain was found not to contribute to RhoA binding. The folded state and thermal stability of the HR1a and HR1b domains were also investigated. HR1b was found to be more thermally stable than HR1a, and it is hypothesized that the differences in the biophysical properties of these two domains govern their interaction with small G proteins.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Hutchinson CL,Lowe PN,McLaughlin SH,Mott HR,Owen Ddoi
10.1021/bi200039usubject
Has Abstractpub_date
2011-04-12 00:00:00pages
2860-9issue
14eissn
0006-2960issn
1520-4995journal_volume
50pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Protein tyrosine kinases (PTKs) are important regulators of mammalian cell function and their own activities are tightly regulated. Underlying their tight regulation, all PTKs contain multiple regulatory domains in addition to a catalytic domain. C-terminal Src kinase (Csk) contains a catalytic domain and a regulatory...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048142j
更新日期：2005-02-08 00:00:00
abstract：:The empoyment of a novel method of affinity chromatography, which makes use of antibodies that specifically bind DNA/RNA hybrids, has made it possible to enrich for rat rDNA molecules which contain R loops formed with the 18S and 28S rRNAs. An approximately 150-fold enrichment of the ratrRNA coding sequences was obtai...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00580a030
更新日期：1979-06-26 00:00:00
abstract：:A set of peptides derived from the N-terminal domain of the ribosomal protein L9 (NTL9) have been characterized in an effort to define the minimum unit of this domain required to fold and to provide model peptides for the analysis of electrostatic interactions in the unfolded state. NTL9 is a 56-residue alpha-beta pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026410c
更新日期：2002-11-12 00:00:00
abstract：:Proteases specific for cleavage after acidic residues have been implicated in several disease states, including epidermolysis, inflammation, and viral processing. A serine protease with specificity toward glutamic acid substrates (Glu-SGP) has been crystallized in the presence of a tetrapeptide ligand and its structur...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00094a001
更新日期：1993-11-02 00:00:00
abstract：:The thermodynamic quantities of the anti-dinitrophenyl antibody-hapten interaction are reported for rabbit, goat, and guinea pig antibodies. Rabbit and goat antibodies had similar exothermic enthalpy changes for their reaction with 2,4-dinitrophenyl-L-lysine (-13.9 and -14.8 kcal/mol, respectively). The enthalpy chang...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00694a032
更新日期：1975-11-18 00:00:00
abstract：:As discovered by Grazi & Trombetta [Grazi, E., & Trombetta, G. (1978) Biochem. J. 175, 361], fructose-1,6-bisphosphate aldolase of rabbit muscle causes the slow formation of inorganic phosphate (Pi) and methylglyoxal when incubated with dihydroxyacetone phosphate (DHAP). In addition, these authors found an acid-labile...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00508a026
更新日期：1981-03-03 00:00:00
abstract：:Helix-helix interactions within membranes are dominated by van der Waals packing motifs and side chain-side chain hydrogen bond formation, which act in tandem to determine the residues that comprise the interface between two given helices. To explore in a systematic manner the tertiary contacts between transmembrane h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0492760
更新日期：2004-11-16 00:00:00
abstract：:FKBP12, a small human enzyme, aids protein folding by catalyzing cis-trans isomerization of peptidyl-prolyl bonds, and is involved in cell signaling pathways, calcium regulation, and the immune response. The underlying molecular mechanisms are not fully understood, but it is well-known that aromatic residues in the ac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b01157
更新日期：2017-01-10 00:00:00
abstract：:Members of the ATP-binding cassette (ABC) transporters share the same basic architecture, with a four-core domain made of two transmembrane plus two nucleotide-binding domains. However, a supramolecular organization has been detected in some ABC transporters, which might be relevant to physiological regulation of subs...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0482809
更新日期：2005-03-22 00:00:00
abstract：:The self-association reaction in which four melittin molecules associate to form an aqueously soluble tetramer was studied by fluorescent spectroscopy. At 23 degrees C, pH 7.15, gamma/2 0.50, the dissociation constant, Kd, is 3.20 x 10(-16) M3. At 23 degrees C, gamma/2 0.60, melittin has an amino acyl group with a pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00272a026
更新日期：1983-02-01 00:00:00
abstract：:This study presents an approach to identifying surface residues on membrane proteins that are exposed toward the membrane-aqueous interface. The method employs a lipid Ni(II) chelate that localizes the metal ion to a region near the membrane-aqueous interface. Lateral diffusion of the lipid chelate results in Heisenbe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015828s
更新日期：2002-01-29 00:00:00
abstract：:cDNAs encoding three cytochrome P-450 enzymes were cloned from a rabbit kidney cDNA library. These three cDNAs exhibit greater than 90% nucleotide sequence identity across the coding region. This degree of sequence identity is also seen with P450IVA4, an enzyme that catalyzes the omega-hydroxylation of prostaglandins ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00456a004
更新日期：1990-01-30 00:00:00
abstract：:The molecular mechanism of hemoglobin cooperativity was studied kinetically by flash photolysis on mixed-state hemoglobins which consist of three ferrous carboxy subunits and one hybrid ferric subunit including fluoromet, azidomet, cyanatomet, and thiocyanatomet. The effects of conformational transitions on the hybrid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00593a012
更新日期：1979-12-25 00:00:00
abstract：:The thylakoid transmembrane DeltapH is the sole energy source driving translocation of precursor proteins by the DeltapH/Tat machinery. Consequently, proton translocation must be coupled to precursor translocation. For the precursor of the 17 kDa protein of the oxygen-evolving complex (pOE17), the protein translocatio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000115f
更新日期：2000-07-18 00:00:00
abstract：:The thermal unfolding of rabbit skeletal heavy meromyosin (HMM), myosin subfragment 1, and subfragment 2 has been studied by differential scanning calorimetry (DSC). Two distinct endotherms are observed in the DSC scan of heavy meromyosin. The first endotherm, with a Tm of 41 degrees C at pH 7.9 in 0.1 M KCl, is assig...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00462a018
更新日期：1990-03-13 00:00:00
abstract：:The Escherichia coli Ada protein repairs methylphosphotriesters in DNA through direct, irreversible transfer to a cysteine residue on the protein, Cys 69. Methylation of Cys 69 increases the sequence-specific DNA-binding activity of Ada by 10(3)-fold, enabling the methylated protein to activate transcription of a meth...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011001m
更新日期：2001-09-25 00:00:00
abstract：:A 19-amino acid residue peptide, Gly-Trp-Leu-Lys-Ile-Lys-Ala-Ala-Met-Arg-Trp-Gly-Phe-Phe-Val-Arg-Lys-Lys- Ala, corresponding to the basic amphiphilic alpha-helix (BAA) motif at the C-terminus of a recombinant tobacco calmodulin-binding protein, TCB60, was synthesized. The interaction of the synthetic binding domain wi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9621674
更新日期：1997-02-25 00:00:00
abstract：:A ribonuclease that hydrolyzes either linear duplex or single-stranded RNA in an exonucleolytic manner has been partially purified from Ehrlich ascites tumor cell nucleoli and is free from other ribonucleases. The enzyme will also degrade the RNA complement of an RNA X DNA duplex; however, no nuclease activity is obse...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00324a022
更新日期：1985-01-29 00:00:00
abstract：:Metal displacement reactions of Cd7MT with Ag+ or Cu+ and interprotein metal exchange reactions between Cd7MT and Ag12MT or Cu12MT were studied by 111Cd NMR. Titration of 111Cd7MT with Ag+ indicates that Ag+ binds preferentially to the beta-domain of the protein to form the metal hybrid species, (Cd4)alpha(Ag6)beta MT...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961401n
更新日期：1996-11-05 00:00:00
abstract：:ALY is a ubiquitously expressed nuclear protein which interacts with proteins such as TAP that are involved in export of mRNA from the nucleus to the cytoplasm, as well as with proteins that bind the T cell receptor alpha gene enhancer. ALY has also been shown to bind mRNA and to co-localize in the nucleus with compon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034062o
更新日期：2003-06-24 00:00:00
abstract：:We have purified the junctional acetylcholine receptor from normal rat skeletal muscle and compared its structure with that of the extrajunctional receptor from denervated muscle. The two receptors from leg muscle were distinguished by isoelectric focusing and by reaction with sera from patients with myasthenia gravis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00582a028
更新日期：1979-07-24 00:00:00
abstract：:The mechanism of bovine intestinal 5'-nucleotide phosphodiesterase was investigated by determining kinetic constants of systematically varied substrates, with emphasis on esters of phosphonic acids (which have much higer Vmax values than conventional phosphodiester substrates), and by pre-steady-state kinetics using b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00625a011
更新日期：1977-03-22 00:00:00
abstract：:Folding experiments have suggested that some proteins have kinetic intermediates with a non-native structure. A simple G ̅o model does not explain such non-native intermediates. Therefore, the folding energy landscape of proteins with non-native intermediates should have characteristic properties. To identify such pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200241a
更新日期：2011-07-26 00:00:00
abstract：:The catalytic mechanism of recombinant soybean cytosolic ascorbate peroxidase (rsAPX) and a derivative of rsAPX in which a cysteine residue (Cys32) located close to the substrate (L-ascorbic acid) binding site has been modified to preclude binding of ascorbate [Mandelman, D., Jamal, J., and Poulos, T. L. (1998) Bioche...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0261591
更新日期：2002-11-19 00:00:00
abstract：:X-ray absorption spectra are reported for the multi-Cu oxidase Rhus vernicifera laccase in oxidized and fully reduced forms and for laccase from which the type 2 Cu has been depleted (T2D). The structure of the Cu K edge for both preparations shows the presence of CuII and CuI in the oxidized and reduced states, respe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00310a008
更新日期：1984-07-17 00:00:00
abstract：:Previous studies have shown that the short-motif electroplax Na channel is phosphorylated in vitro by cyclic AMP-dependent protein kinase (PKA) at serines 6 or 7 and 1776 and threonine 17 (Emerick & Agnew, 1989). We here show that phosphatase treatment of solubilized, purified Na channels enhanced subsequent PKA label...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00087a023
更新日期：1993-09-14 00:00:00
abstract：:The contribution of backbone hydrogen bonds in alpha-helices to the overall stability of a protein has been examined experimentally by replacing several backbone amide linkages in alpha-helix 39-50 of T4 lysozyme with ester linkages. T4 lysozyme variants wherein the backbone amide bonds between residues Ser38 and Leu3...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9707685
更新日期：1997-09-23 00:00:00
abstract：:Several recent studies have provided evidence that LIN28, a cytoplasmic RNA-binding protein, inhibits the biogenesis of members of the let-7 microRNA family at the Dicer step in both mammals and Caenorhabditis elegans. However, the precise mechanism of inhibition is still poorly understood. Here we report on an in vit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200851d
更新日期：2011-09-06 00:00:00
abstract：:The carboxymethylation of RNase T1 at the gamma-carboxyl group of Glu58 leads to a complete loss of the enzymatic activity while it retains substrate-binding ability. Accompanying the carboxymethylation, RNase T1 undergoes a remarkable thermal stabilization of 9 degrees C in the melting temperature (Tm). In order to c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960493d
更新日期：1996-06-25 00:00:00
abstract：:Growth factor receptor-bound protein 14 (Grb14) is involved in growth factor receptor tyrosine kinase signaling. Here we report that light causes a major redistribution of Grb14 among the individual subcellular compartments of the retinal rod photoreceptor. Grb14 is localized predominantly to the inner segment, nuclea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9000062
更新日期：2009-06-23 00:00:00
