Biochemical characterization of the HpxO enzyme from Klebsiella pneumoniae, a novel FAD-dependent urate oxidase.

abstract：:Soluble inorganic pyrophosphatase (PPase) is one of the better understood phosphoryl-transfer enzymes and is distinctive in having four divalent metal ions at the active site. Here we determine pH profiles for wild-type Saccharomyces cerevisiae PPase (Y-PPase) and for 14 of its active site variants and consider the ef...

journal_title：Biochemistry

authors： Pohjanjoki P,Lahti R,Goldman A,Cooperman BS

更新日期：1998-02-17 00:00:00

abstract：:Beta2microglobulin (beta2m) is the major protein component of the fibrillar amyloid deposits isolated from patients diagnosed with dialysis-related amyloidosis (DRA). While investigating the molecular mechanism of amyloid fibril formation by beta2m, we found that the beta2m C-terminal peptide of 28 residues (cbeta2m) ...

journal_title：Biochemistry

authors： Ivanova MI,Gingery M,Whitson LJ,Eisenberg D

更新日期：2003-11-25 00:00:00

abstract：:The monoamine transporter of the chromaffin granule membranes can be specifically labeled by the photoaffinity reagent 7-azido-8-[125I]iodoketanserin. The characteristics of the labeled protein have been investigated. Two-dimensional gel electrophoresis of the labeled membranes indicated a MW of about 70,000 and an is...

journal_title：Biochemistry

authors： Isambert MF,Gasnier B,Botton D,Henry JP

更新日期：1992-02-25 00:00:00

abstract：:Eubacterial peptidyl-tRNA hydrolase (PTH) recycles all N-blocked aminoacyl-tRNA molecules but initiator formyl-methionyl-tRNAfMet, the acceptor helix of which is characterized by a 1-72 mismatch. Positive selection by PTH of noninitiator tRNA molecules with a full 1-72 base pair is abolished, however, upon the removal...

journal_title：Biochemistry

authors： Fromant M,Plateau P,Schmitt E,Mechulam Y,Blanquet S

更新日期：1999-04-20 00:00:00

abstract：:Aerobic and facultative bacteria and archaea harboring mer loci exhibit resistance to the toxic effects of Hg(II) and organomercurials [RHg(I)]. In broad spectrum resistance, RHg(I) is converted to less toxic Hg(0) in the cytosol by the sequential action of organomercurial lyase (MerB: RHg(I) → RH + Hg(II)) and mercur...

journal_title：Biochemistry

authors： Hong B,Nauss R,Harwood IM,Miller SM

更新日期：2010-09-21 00:00:00

abstract：:To probe the mechanism of the reversible DNA phosphodiester bond cleavage and religation mechanism of the type I topoisomerase from vaccinia virus, we have synthesized DNA substrates carrying a single nonbridging Rp- or Sp-phosphorothioate (Ps) modification at the scissile phosphodiester (Pd) bond. Analysis of the ste...

journal_title：Biochemistry

authors： Stivers JT,Jagadeesh GJ,Nawrot B,Stec WJ,Shuman S

更新日期：2000-05-09 00:00:00

abstract：:In the accompanying paper [Suzuki, A., Yamazaki, M., & Ito, T. (1996) Biochemistry 35, 5238-5244], we presented a quantitative phase diagram of actin filament ( (F-actin) described with the F-actin concentration and delta chi value which characterizes the affinity of F-actin with solvent. The phase diagram shows that ...

journal_title：Biochemistry

authors： Suzuki A,Ito T

更新日期：1996-04-23 00:00:00

abstract：:Clustering of anionic lipids away from zwitterionic ones by cationic antimicrobial agents has recently been established as a mechanism of action of natural small, flexible peptides as well as non-natural synthetic peptide mimics. One of the largest classes of antimicrobial peptides consists of peptides that form catio...

journal_title：Biochemistry

authors： Epand RF,Maloy WL,Ramamoorthy A,Epand RM

更新日期：2010-05-18 00:00:00

abstract：:A set of peptides derived from the N-terminal domain of the ribosomal protein L9 (NTL9) have been characterized in an effort to define the minimum unit of this domain required to fold and to provide model peptides for the analysis of electrostatic interactions in the unfolded state. NTL9 is a 56-residue alpha-beta pro...

journal_title：Biochemistry

authors： Horng JC,Moroz V,Rigotti DJ,Fairman R,Raleigh DP

更新日期：2002-11-12 00:00:00

abstract：:We studied the interactions between the SH2 domain of growth factor receptor binding protein 2 (Grb2) and ErbB receptor-derived phosphotyrosyl peptides using molecular dynamics, free energy calculations, and surface plasmon resonance (SPR) analysis. Binding free energies for nine phosphotyrosyl peptides were calculate...

journal_title：Biochemistry

authors： Suenaga A,Hatakeyama M,Ichikawa M,Yu X,Futatsugi N,Narumi T,Fukui K,Terada T,Taiji M,Shirouzu M,Yokoyama S,Konagaya A

更新日期：2003-05-13 00:00:00

abstract：:Protein splicing is a self-catalyzed post-translational modification in which the intein enzyme excises itself from a precursor protein and ligates the flanking sequences to produce a mature protein. We report the solution structure of a 136-residue DnaX mini-intein enzyme derived from the cyanobacterium Spirulina pla...

journal_title：Biochemistry

authors： Boral S,Maiti S,Basak AJ,Lee W,De S

更新日期：2020-12-22 00:00:00

abstract：:Arginase is a binuclear Mn(2+) metalloenzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea. X-ray crystal structures of arginase complexed to substrate analogues N(omega)-hydroxy-L-arginine and N(omega)-hydroxy-nor-L-arginine, as well as the products L-ornithine and urea, complete a set of struct...

journal_title：Biochemistry

authors： Cox JD,Cama E,Colleluori DM,Pethe S,Boucher JL,Mansuy D,Ash DE,Christianson DW

更新日期：2001-03-06 00:00:00

abstract：:Hydrolysis of monodisperse short chain phosphatidylcholines, far below their critical micelle concentration, by phospholipase A2 (PLA2) and other interfacial enzymes is characterized. Results show that virtually all the observed hydrolysis by pancreatic and human inflammatory PLA2 occurs on surfaces of the reaction ve...

journal_title：Biochemistry

authors： Yu BZ,Berg OG,Jain MK

更新日期：1999-08-10 00:00:00

abstract：:The flavoenzyme tryptophan 2-monooxygenase catalyzes the oxidation of tryptophan to indoleacetamide, carbon dioxide, and water. The enzyme is a homologue of l-amino acid oxidase. In the structure of l-amino acid oxidase complexed with aminobenzoate, Tyr372 hydrogen bonds with the carboxylate of the inhibitor in the ac...

journal_title：Biochemistry

authors： Sobrado P,Fitzpatrick PF

更新日期：2003-12-02 00:00:00

abstract：:2,3-Butanedione monoxime (BDM) reversibly inhibits force production in muscle. At least part of its action appears to be directly on the contractile apparatus. To understand better its mechanism of action, we studied the effect of BDM on the steps of myosin subfragment 1 Mg(2+)-ATPase in 0.1 M potassium acetate, pH 7....

journal_title：Biochemistry

authors： Herrmann C,Wray J,Travers F,Barman T

更新日期：1992-12-08 00:00:00

abstract：:RecG is a DNA helicase involved in the repair of damage at a replication fork and catalyzes the reversal of the fork to a point beyond the damage in the template strand. It unwinds duplex DNA in reactions that are coupled to ATP hydrolysis. The kinetic mechanism of duplex DNA unwinding by RecG was analyzed using a qua...

journal_title：Biochemistry

authors： Martinez-Senac MM,Webb MR

更新日期：2005-12-27 00:00:00

abstract：:Amphiphiles respond both to polar and to nonpolar solvents. In this paper X-ray diffraction and osmotic stress have been used to examine the phase behavior, the structural dimensions, and the work of deforming the monolayer-lined aqueous cavities formed by mixtures of dioleoylphosphatidylethanolamine (DOPE) and dioleo...

journal_title：Biochemistry

authors： Rand RP,Fuller NL,Gruner SM,Parsegian VA

更新日期：1990-01-09 00:00:00

abstract：:In the Arc repressor dimer, the side chains of Ile37 and Val41 in alpha-helix B pack against each other and against the symmetry-related side chains of Ile37' and Val41' in alpha-helix B' to form part of the hydrophobic core and the dimer interface. Following combinatorial mutagenesis of these positions, only the wild...

journal_title：Biochemistry

authors： Milla ME,Sauer RT

更新日期：1995-03-14 00:00:00

abstract：:A study of the dynamics and mechanism of the various thermotropic phase transitions undergone by the hydrated monoacylglycerides monoolein and monoelaidin, in the temperature range of 20-120 degrees C and from 0 to 5 M NaCl, has been undertaken. Measurements were made by using time-resolved X-ray diffraction at the Co...

journal_title：Biochemistry

authors： Caffrey M

更新日期：1987-10-06 00:00:00

abstract：:TAR DNA-binding protein 43 (TDP-43) has been identified as the major constituent of the proteinaceous inclusions that are characteristic of most forms of amyotrophic lateral sclerosis (ALS) and ubiquitin positive frontotemporal lobar degeneration (FTLD). Wild type TDP-43 inclusions are a pathological hallmark of >95% ...

journal_title：Biochemistry

authors： Ng JSW,Hanspal MA,Matharu NS,Barros TP,Esbjörner EK,Wilson MR,Yerbury JJ,Dobson CM,Kumita JR

更新日期：2019-10-01 00:00:00

abstract：:The global transcription factor, p53, is a master regulator of gene expression in cells. Mutations in the TP53 gene promote unregulated cell growth through the inactivation of downstream effectors of the p53 pathway. In fact, mutant p53 is highly prone to misfolding and frequently resides inside the cell as large aggr...

journal_title：Biochemistry

authors： Navalkar A,Ghosh S,Pandey S,Paul A,Datta D,Maji SK

更新日期：2020-01-21 00:00:00

abstract：:The NADH absorbance spectrum of nicotinoprotein (NADH-containing) alcohol dehydrogenase from Amycolatopsis methanolica has a maximum at 326 nm. Reduced enzyme-bound pyridine dinucleotide could be reversibly oxidized by acetaldehyde. The fluorescence excitation spectrum for NADH bound to the enzyme has a maximum at 325...

journal_title：Biochemistry

authors： Piersma SR,Visser AJ,de Vries S,Duine JA

更新日期：1998-03-03 00:00:00

abstract：:We mutated residue 166, located in the putative Na(+) transport pathway between transmembrane segments 4 and 5 of human Na(+)/glucose cotransporter (hSGLT1), from alanine to cysteine (A166C). A166C was expressed in Xenopus laevis oocytes, and electrophysiological methods were used to assay function. The affinity for N...

journal_title：Biochemistry

authors： Meinild AK,Loo DD,Hirayama BA,Gallardo E,Wright EM

更新日期：2001-10-02 00:00:00

abstract：:Membrane-bound guanylate cyclases harbor a region called the dimerization or linker domain, which aids the enzymes in adopting an optimal monomer-monomer arrangement for catalysis. One subgroup of these guanylate cyclases is expressed in rod and cone cells of vertebrate retina, and mutations in the dimerization domain...

journal_title：Biochemistry

authors： Zägel P,Dell'Orco D,Koch KW

更新日期：2013-07-30 00:00:00

abstract：:The Haemophilus influenzae diaminopimelate epimerase was cloned, expressed, purified, and crystallized in the C2221 space group (a = 102.1 A, b = 115.4 A, c = 66.3 A, alpha = beta = gamma = 90 degrees). The three-dimensional structure was solved to 2.7 A using a single Pt derivative and the Se-Met-substituted enzyme t...

journal_title：Biochemistry

authors： Cirilli M,Zheng R,Scapin G,Blanchard JS

更新日期：1998-11-24 00:00:00

abstract：:Prokaryotic promoters have been extensively studied to relate sequence features to promoter function. Here we examine the relationship between double-helix stability and promoter activity. The double-helix stability is evaluated from sequence data by free energy computation, based on reported values of dinucleotide fr...

journal_title：Biochemistry

authors： Margalit H,Shapiro BA,Nussinov R,Owens J,Jernigan RL

更新日期：1988-07-12 00:00:00

abstract：:Quorum sensing, the ability of bacteria to sense their own population density through the synthesis and detection of small molecule signals, has received a great deal of attention in recent years. Acyl homoserine lactones (AHLs) are a major class of quorum sensing signaling molecules. In nature, some bacteria that do ...

journal_title：Biochemistry

authors： Chowdhary PK,Keshavan N,Nguyen HQ,Peterson JA,González JE,Haines DC

更新日期：2007-12-18 00:00:00

abstract：:Recent crystallographic data suggest that conserved residues in the connection subdomain and C-terminal ribonuclease H (RNase H) domain of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) contact the nascent DNA primer and modulate the trajectory of the template relative to the RNase H catalytic ce...

journal_title：Biochemistry

authors： Rausch JW,Lener D,Miller JT,Julias JG,Hughes SH,Le Grice SF

更新日期：2002-04-16 00:00:00

abstract：:Glycosylation is a common modification that can endow proteins with altered physical and biological properties. Ribonuclease 1 (RNase 1), which is the human homologue of the archetypal enzyme RNase A, undergoes N-linked glycosylation at asparagine residues 34, 76, and 88. We have produced the three individual glycofor...

journal_title：Biochemistry

authors： Kilgore HR,Latham AP,Ressler VT,Zhang B,Raines RT

更新日期：2020-09-01 00:00:00

abstract：:In recent years, there has been dramatic growth in the study of RNA. RNA has gone from being known as an intermediate in the central dogma of molecular biology to a molecule with a large diversity of structure and function that is involved in all aspects of biology. As new functions are rapidly discovered, it has beco...

journal_title：Biochemistry

authors： Moon MH,Hilimire TA,Sanders AM,Schneekloth JS Jr

更新日期：2018-08-07 00:00:00