Role of the C-terminal 28 residues of beta2-microglobulin in amyloid fibril formation.

abstract：:Several snake venoms contain a phospholipase A2 in which position 49 in the active site is occupied by a lysine or a serine instead of the aspartate residue normally found. Although these proteins do not bind Ca2+ and are devoid of catalytic activity, they are still highly specific myotoxins and have recently been sho...

journal_title：Biochemistry

authors： Pedersen JZ,Lomonte B,Massoud R,Gubensek F,Gutiérrez JM,Rufini S

更新日期：1995-04-11 00:00:00

abstract：:CC chemokine receptor 5 (CCR5) is the receptor for several inflammatory chemokines and is a coreceptor for HIV-1. Posttranslational sulfation of tyrosines in the N-terminal regions of chemokine receptors has been shown to be important in the binding affinity for chemokine ligands. In addition, sulfation of CCR5 is cru...

journal_title：Biochemistry

authors： Jen CH,Moore KL,Leary JA

更新日期：2009-06-16 00:00:00

abstract：:Frequency-resolved fluorescence measurements have been performed to quantitate the lateral stress of the lipid layer containing nonbilayer phase preferring dioleoylphosphatidylethanolamine (DOPE). On the basis of a new rotational diffusion model, the wobbling diffusion constant (Dw), the curvature-related hopping diff...

journal_title：Biochemistry

authors： Chen SY,Cheng KH,Van der Meer BW

更新日期：1992-04-21 00:00:00

abstract：:When the single-stranded RNA genome of HIV-1 is copied into double-stranded DNA, the viral enzyme reverse transcriptase (RT) catalyzes the addition of approximately 20,000 nucleotides; however, the precise mechanism of nucleotide addition is unknown. In this study, we attempt to integrate the genetic data and biochemi...

journal_title：Biochemistry

authors： Patel PH,Jacobo-Molina A,Ding J,Tantillo C,Clark AD Jr,Raag R,Nanni RG,Hughes SH,Arnold E

更新日期：1995-04-25 00:00:00

abstract：:Superoxide reductase (SOR) is an Fe protein that catalyzes the reduction of superoxide to give H(2)O(2). Recently, the mutation of the Glu47 residue into alanine (E47A) in the active site of SOR from Desulfoarculus baarsii has allowed the stabilization of an iron-peroxo species when quickly reacted with H(2)O(2) [Math...

journal_title：Biochemistry

authors： Horner O,Mouesca JM,Oddou JL,Jeandey C,Nivière V,Mattioli TA,Mathé C,Fontecave M,Maldivi P,Bonville P,Halfen JA,Latour JM

更新日期：2004-07-13 00:00:00

abstract：:Furanacryloyl-Phe-Gly-Gly has been shown to be a convenient substrate for angiotensin converting enzyme (dipeptidyl carboxypeptidase, EC 3.4.15.1). A detailed kinetic analysis of the hydrolysis of this substrate indicates normal Michaelis-Menten behavior with kcat = 19000 min-1 and KM = 3.0 x 10(-4) M determined at pH...

journal_title：Biochemistry

authors： Bünning P,Holmquist B,Riordan JF

更新日期：1983-01-04 00:00:00

abstract：:Low molecular weight protein tyrosine phosphatases (LMW-PTPs) are small enzymes that are ubiquitous in many organisms. They are important in biological processes such as cell proliferation, adhesion, migration, and invasiveness. LMW-PTP is expressed in mammalian cells as two isoforms (IF1 and IF2) originating through ...

journal_title：Biochemistry

authors： Caselli A,Taddei ML,Bini C,Paoli P,Camici G,Manao G,Cirri P,Ramponi G

更新日期：2007-05-29 00:00:00

abstract：:Sf9 cells infected with a recombinant baculovirus containing the gene for human prorenin were cultured in the presence of [3H]mannose. In vivo labeled prorenin was isolated by immunoprecipitation from the culture medium and digested with Pronase. The oligosaccharide structures on the resulting glycopeptides were analy...

journal_title：Biochemistry

authors： Aeed PA,Elhammer AP

更新日期：1994-07-26 00:00:00

abstract：:The mechanism by which acyl-CoA dehydrogenases initiate catalysis was studied by using p-substituted phenylacetyl-CoAs (substituents-NO2, -CN, and CH3CO-), 3S-C8-, and 3'-dephospho-3S-C8CoA. These analogues lack a beta C-H and cannot undergo alpha,beta-dehydrogenation. Instead they deprotonate at alpha C-H at pH > or ...

journal_title：Biochemistry

authors： Vock P,Engst S,Eder M,Ghisla S

更新日期：1998-02-17 00:00:00

abstract：:Cells cultured from human skin synthesize precursor forms of types I and III collagens. After denaturation and reduction, the polypeptide chains obtained from these molecules had molecular weights estimated to be 140,000 and 120, 000. The larger chains, the pro alpha chains, are believed to be derived from the origina...

journal_title：Biochemistry

authors： Lichtenstein JR,Byers PH,Smith BD,Martin GR

更新日期：1975-04-22 00:00:00

abstract：:Peptide deformylase (EC 3.5.1.31) catalyzes the removal of a formyl group from the N-termini of nascent ribosome-synthesized polypeptides, an obligatory step during protein maturation in eubacteria. Since its discovery in crude Escherichia coli extracts 3 decades ago, the deformylase has resisted all attempts of purif...

journal_title：Biochemistry

authors： Rajagopalan PT,Datta A,Pei D

更新日期：1997-11-11 00:00:00

abstract：:Volumetric characteristics of protein recognition events determine the direction of pressure-induced shifts in the recognition reaction, while also providing insights into the structural, dynamic, and hydration changes. We report changes in volume, ΔV, and adiabatic compressibility, ΔK(S), accompanying the binding of ...

journal_title：Biochemistry

authors： Son I,Shek YL,Dubins DN,Chalikian TV

更新日期：2012-07-24 00:00:00

abstract：:SecA-lipid interactions are believed to be important for the translocation of precursor proteins across the inner membrane of Escherichia coli [Lill, R., Dowhan, W., & Wickner, W. (1990) Cell 60, 271-280]. SecA insertion into the phospholipid bilayer could a role in this process. We investigated this possibility by st...

journal_title：Biochemistry

authors： Breukink E,Demel RA,de Korte-Kool G,de Kruijff B

更新日期：1992-02-04 00:00:00

abstract：:Intramolecular excimer formation with 1,3-di(1-pyrenyl)propane was used to probe the fluidity of sarcoplasmic reticulum (SR) membranes where the probe could be incorporated (in a probe/lipid molar ratio of 1/2000 or less) without inducing any detectable damage. The temperature was varied between -1 and 40 degrees C. T...

journal_title：Biochemistry

authors： Almeida LM,Vaz WL,Zachariasse KA,Madeira VM

更新日期：1982-11-09 00:00:00

abstract：:The initial step of infection of blood cells with the human immunodeficiency virus, HIV, is the formation of a complex of the viral envelope protein gp120 and its human receptor CD4. We have examined structural features of recombinant soluble CD4 (sCD4) by chemical modification of arginine residues with hydroxyphenylg...

journal_title：Biochemistry

authors： Hager-Braun C,Tomer KB

更新日期：2002-02-12 00:00:00

abstract：:The luminescence properties (fluorescence and phosphorescence) of luliberin have been investigated at liquid-nitrogen temperature (77 K) in 50% ethylene glycolaqueous buffer at various pH's. Calculation of the energy-transfer efficiency between Trp and Tyr residues leads to an evaluation of the distance separating the...

journal_title：Biochemistry

authors： Marche P,Montenay-Garestier T,Fromageot P,Hélène C

更新日期：1976-12-28 00:00:00

abstract：:The association of ferredoxin with ferredoxin:NADP+ reductase (both proteins from spinach chloroplasts) was characterized by isothermal titration calorimetry and fluorescence quenching titration. The formation of the complex is mainly driven by a positive entropy change (delta S = 125 +/- 8 J mol-1 K-1). The calorimet...

journal_title：Biochemistry

authors： Jelesarov I,Bosshard HR

更新日期：1994-11-15 00:00:00

abstract：:To test the prediction that extensively fluorinated (fluorous) proteins should be more stable and exhibit novel self-segregating behavior, the properties of the de novo designed model 4-alpha-helix bundle protein, alpha 4F 6, in which the hydrophobic core is packed entirely with the extensively fluorinated amino acid ...

journal_title：Biochemistry

authors： Gottler LM,de la Salud-Bea R,Marsh EN

更新日期：2008-04-15 00:00:00

abstract：:Fourteen oligonucleotides 8-21 nucleotides in length and their complements were synthesized as DNA and RNA. For each sequence, four kinds of duplexes, DNA:DNA, RNA:RNA, DNA:RNA, and RNA:DNA, were prepared. Twelve sequences had A.T/U content varying from 25 to 80% and dPy content in the DNA strands varying from 0 to 10...

journal_title：Biochemistry

authors： Lesnik EA,Freier SM

更新日期：1995-08-29 00:00:00

abstract：:Murine double-minute clone 2 protein (MDM2) is an E3 ubiquitin ligase that regulates the turnover of several cellular factors including the p53 tumor suppressor protein. As part of the mechanism of p53 induction in response to DNA damage, a cluster of serine residues within the central acidic domain of MDM2 become hyp...

journal_title：Biochemistry

authors： Winter M,Milne D,Dias S,Kulikov R,Knippschild U,Blattner C,Meek D

更新日期：2004-12-28 00:00:00

abstract：:The betagamma-crystallin superfamily consists of evolutionarily related proteins with domain topology similar to lens beta- and gamma-crystallins, formed from duplicated Greek key motifs. Ca(2+) binding was found in a few betagamma-crystallin members earlier, although its prevalence and diversity as inherent molecular...

journal_title：Biochemistry

authors： Aravind P,Mishra A,Suman SK,Jobby MK,Sankaranarayanan R,Sharma Y

更新日期：2009-12-29 00:00:00

abstract：:Thermal unfolding/refolding studies of the three tropomyosin dimers, alpha alpha, alpha beta, and beta beta, from chicken gizzard muscle were performed to explain the preferential assembly of alpha- and beta-tropomyosin subunits into heterodimers, alpha beta [Lehrer, S. S., & Qian, Y. (1989) J. Biol. Chem. 265, 1134]....

journal_title：Biochemistry

authors： Lehrer SS,Stafford WF 3rd

更新日期：1991-06-11 00:00:00

abstract：:Channelrhodopsins (ChRs) from green flagellate algae function as light-gated ion channels when expressed heterologously in mammalian cells. Considerable interest has focused on understanding the molecular mechanisms of ChRs to bioengineer their properties for specific optogenetic applications such as elucidating the f...

journal_title：Biochemistry

authors： Ogren JI,Yi A,Mamaev S,Li H,Lugtenburg J,DeGrip WJ,Spudich JL,Rothschild KJ

更新日期：2015-01-20 00:00:00

abstract：:The interaction of solvent of the substrate binding domain of the bacterial heat shock 70 chaperone protein DnaK was studied in its apo form and with bound hydrophobic substrate peptide, using refined nuclear magnetic resonance experiments. Distinct differences between the two states of the protein were observed. Acco...

journal_title：Biochemistry

authors： Cai S,Stevens SY,Budor AP,Zuiderweg ER

更新日期：2003-09-30 00:00:00

abstract：:Nuclease footprinting techniques have been employed to investigate the interaction between the diuretic drug amiloride, a sodium channel blocker with potential therapeutic use in the treatment of cystic fibrosis, and three DNA fragments of defined sequence. Using either DNAse I or micrococcal nuclease as probes, an un...

journal_title：Biochemistry

authors： Bailly C,Cuthbert AW,Gentle D,Knowles MR,Waring MJ

更新日期：1993-03-16 00:00:00

abstract：:Pyruvate formate-lyase activating enzyme (PFL-AE) catalyzes the generation of a catalytically essential glycyl radical on pyruvate formate-lyase (PFL). Purified PFL-AE contains an oxygen-sensitive, labile [4Fe-4S] cluster that undergoes cluster interconversions in vitro, with only the [4Fe-4S](+) cluster state being c...

journal_title：Biochemistry

authors： Yang J,Naik SG,Ortillo DO,García-Serres R,Li M,Broderick WE,Huynh BH,Broderick JB

更新日期：2009-10-06 00:00:00

abstract：:Cellular Ca2+ transients and Ca2+-binding proteins regulate physiological phenomena as diverse as muscle contraction, neurosecretion, and cell division. When Ca2+ is rapidly mixed with slow Ca2+ chelators, EGTA, or Mg2+/EDTA, artificial Ca2+ transients (ACTs) of varying duration (0.1-50 ms half-widths (hws)) and ampli...

journal_title：Biochemistry

authors： Davis JP,Tikunova SB,Walsh MP,Johnson JD

更新日期：1999-03-30 00:00:00

abstract：:The conformation of two fragments derived from anti-poly(D-alanyl) antibodies, the divalent fragment (Fab')2 and the monovalent fragment Fab', was studied by small-angle X-ray scattering before and after interaction with the tetra-D-alanine amide hapten. More than 90% of the combining sites were occupied by the hapten...

journal_title：Biochemistry

authors： Pilz I,Kratky O,Licht A,Sela M

更新日期：1975-03-25 00:00:00

abstract：:Structures of glycopeptides obtained by exhaustive Pronase digestion of high molecular weight (1.7 X 10(5)) salmon egg polysialoglycoprotein have been elucidated. Six principal glycopeptides isolated by gel chromatography and DEAE-Sephadex A-25 chromatography in the absence or presence of borate ion were analyzed for ...

journal_title：Biochemistry

authors： Shimamura M,Inoue Y,Inoue S

更新日期：1985-09-24 00:00:00

abstract：:Effects of paramagnetic shift reagents on the 13C NMR spectra obtained from single-walled vesicle dispersions of egg phosphatidylcholine enriched with 13C in the N-methyl carbons are investigated. Spectra obtained at 25.1 MHz show that, at Yb3+ to phospholipid molar ratios as low as 0.06, complete resolution of the N-...

journal_title：Biochemistry

authors： Sears B,Hutton WC,Thompson TE

更新日期：1976-04-20 00:00:00