Modification of Rhodospirillum rubrum ribulose bisphosphate carboxylase with pyridoxal phosphate. 2. Stoichiometry and kinetics of inactivation.

abstract：:Glutaminase (GLS1/2) catalyzes the conversion of L-glutamine to L-glutamate and ammonia. The level of a splice variant of GLS1 (GAC) is elevated in certain cancers, and GAC is specifically inhibited by bis-2-(5-phenylacetimido-1,2,4,thiadiazol-2-yl)ethyl sulfide (BPTES). We report here the first full-length crystal st...

journal_title：Biochemistry

authors： DeLaBarre B,Gross S,Fang C,Gao Y,Jha A,Jiang F,Song J J,Wei W,Hurov JB

更新日期：2011-12-20 00:00:00

abstract：:This research was undertaken to distinguish between local and global unfolding in the reversible thermal denaturation of bovine pancreatic ribonclease A (RNase A). Local unfolding was monitored by steady-state and time-resolved fluorescence of nine mutants in each of which a single tryptophan was substituted for a wil...

journal_title：Biochemistry

authors： Navon A,Ittah V,Laity JH,Scheraga HA,Haas E,Gussakovsky EE

更新日期：2001-01-09 00:00:00

abstract：:We have recently described a procedure for the purification of microtubule associated protein 1B (MAP1B) from calf brain [Pedrotti, B., & Islam K. (1995) Cell Motil. Cytoskeleton 30, 301-309], and this study further characterizes the purified protein and its interaction with microtubules. We show that purified MAP1B (...

journal_title：Biochemistry

authors： Pedrotti B,Ulloa L,Avila J,Islam K

更新日期：1996-03-05 00:00:00

abstract：:A 16-base pair oligo(purine)-oligo(pyrimidine) sequence present in the coding region of two HIV 1 proviral genes (pol and nef) was chosen as a target for triplex-forming oligonucleotides in in vitro transcription assays. Inhibition of transcription elongation was observed with triplex-forming oligonucleotide-acridine ...

journal_title：Biochemistry

authors： Giovannangeli C,Perrouault L,Escudé C,Nguyen T,Hélène C

更新日期：1996-08-13 00:00:00

abstract：:The topography of the photosynthetic reaction center (RC) polypeptides (H, M, and L) was investigated by proteolysis and radioiodination of membrane vesicles isolated from Rhodopseudomonas sphaeroides. Chromatophores, obtained from French-pressed cell lysates, are closed vesicles' and oriented inside out with respect ...

journal_title：Biochemistry

authors： Bachmann RC,Gillies K,Takemoto JY

更新日期：1981-08-04 00:00:00

abstract：:The rate of 1-palmitoyl-2-[12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino] dodecanoyl] phosphatidylcholine (P-C12-NBD-PC) transfer between dioleoylphosphatidylcholine vesicles was measured by a technique based on resonance energy transfer between P-C12-NBD-PC and N-(lissamine rhodamine B sulfonyl)dioleoylphosphatidyletha...

journal_title：Biochemistry

authors： Nichols JW

更新日期：1986-08-12 00:00:00

abstract：:Streptococcus pneumoniae 5'-methylthioadenosine/S-adenosylhomocysteine hydrolase (MTAN) catalyzes the hydrolytic deadenylation of its substrates to form adenine and 5-methylthioribose or S-ribosylhomocysteine (SRH). MTAN is not found in mammals but is involved in bacterial quorum sensing. MTAN gene disruption affects ...

journal_title：Biochemistry

authors： Singh V,Shi W,Almo SC,Evans GB,Furneaux RH,Tyler PC,Painter GF,Lenz DH,Mee S,Zheng R,Schramm VL

更新日期：2006-10-31 00:00:00

abstract：:By use of site-directed mutagenesis, each prolyl residue in the lac permease of Escherichia coli at positions 28 (putative helix I), 31 (helix I), 61 (helix II), 89 (helix III), 97 (helix III), 123 (helix IV), 192 (putative hydrophilic region 7), 220 (helix VII), 280 (helix VIII), and 327 [helix X; Lolkema, J. S., et ...

journal_title：Biochemistry

authors： Consler TG,Tsolas O,Kaback HR

更新日期：1991-02-05 00:00:00

abstract：:Human methionine synthase reductase (MSR), a diflavin oxidoreductase, plays a vital role in methionine and folate metabolism by sustaining methionine synthase (MS) activity. MSR catalyzes the oxidation of NADPH and shuttles electrons via its FAD and FMN cofactors to inactive MS-cob(II)alamin. A conserved aromatic resi...

journal_title：Biochemistry

authors： Meints CE,Gustafsson FS,Scrutton NS,Wolthers KR

更新日期：2011-12-27 00:00:00

abstract：:Recently we demonstrated that the unique stereoelectronic relationships inherent in the structure of plasmenylethanolamine facilitate membrane fusion, and we postulated the existence of a membrane fusion protein which could exploit the propensity of plasmenylethanolamine molecular species to adapt an inverted hexagona...

journal_title：Biochemistry

authors： Glaser PE,Gross RW

更新日期：1995-09-26 00:00:00

abstract：:The periplasmic protein CusF, as a part of the CusCFBA efflux complex, plays a role in resistance to elevated levels of copper and silver in Escherichia coli. Although homologues have been identified in other Gram-negative bacteria, the substrate of CusF and its precise role in metal resistance have not been described...

journal_title：Biochemistry

authors： Kittleson JT,Loftin IR,Hausrath AC,Engelhardt KP,Rensing C,McEvoy MM

更新日期：2006-09-19 00:00:00

abstract：:Clostridial neurotoxins are zinc endopeptidases, and each contains one Zn(2+)/molecule. To investigate the structural/functional role of Zn(2+) in botulinum neurotoxin light chain (the enzymatic subunit of the neurotoxin), the effect of the removal of zinc on protein folding and enzyme kinetics was investigated. The a...

journal_title：Biochemistry

authors： Li L,Singh BR

更新日期：2000-08-29 00:00:00

abstract：:Hepatic mixed-function oxidase metabolism of the ubiquitous pollutant polychlorinated biphenyls (PCBs) is implicated in their toxification and detoxification. We used dichlorobiphenyls (DCBs) as models to investigate the effect of the chloro substituent sites on this metabolism experimentally and by molecular orbital ...

journal_title：Biochemistry

authors： Kaminsky LS,Kennedy MW,Adams SM,Guengerich FP

更新日期：1981-12-22 00:00:00

abstract：:The blood-clotting protein fibrinogen is composed of three subunits, designated A alpha, B beta, and gamma, which are encoded by a family of related genes. As part of the acute-phase response, expression of the fibrinogen genes is coordinately regulated in the liver by glucocorticoids. To understand the factors underl...

journal_title：Biochemistry

authors： Roberts LR,Nichols LA,Holland LJ

更新日期：1993-11-02 00:00:00

abstract：:In the presence of Ca2+ norchlorpromazine isothiocyanate forms a monocovalent complex with calmodulin: CAPP1-calmodulin (Newton et al, 1983). Trypsin digestion of [3H]CAPP1-calmodulin yields as the major radioactive peptide N epsilon-CAPP-Lys-Met-Lys, corresponding to residues 75-77 of calmodulin. Stoichiometric amoun...

journal_title：Biochemistry

authors： Newton DL,Klee CB

更新日期：1989-05-02 00:00:00

abstract：:Site-specific mutagenesis has been used to create two mutant versions of aspartate transcarbamoylase. Arg-167 and Gln-231, both previously identified as interacting with the portion of the bisubstrate analogue N-(phosphonoacetyl)-L-aspartate (PALA) that corresponds to aspartate [Krause, K. L., Voltz, K. W., & Lipscomb...

journal_title：Biochemistry

authors： Stebbins JW,Zhang Y,Kantrowitz ER

更新日期：1990-04-24 00:00:00

abstract：:Induced fit has been postulated to be an important component of ligand interactions with proteins, including protein-DNA interactions. We imagined that the entropic cost of induced fit might be highly dependent on the local protein sequence context around critical contact residues. To investigate this question, we ana...

journal_title：Biochemistry

authors： Ribas de Pouplana L,Auld DS,Kim S,Schimmel P

更新日期：1996-06-25 00:00:00

abstract：:X-ray studies of the proofreading 3',5'-exonuclease site of the large (Klenow) fragment of DNA polymerase I have detected a binuclear metal complex consisting of a pentacoordinate metal (site A) which shares a ligand, Asp-355, with an octahedral metal (site B) [Freemont, P. S., Friedman, J. M., Beese, L. S., Sanderson...

journal_title：Biochemistry

authors： Han H,Rifkind JM,Mildvan AS

更新日期：1991-11-19 00:00:00

abstract：:High-resolution 2D NMR spectra of the duplex CGCGAATTCGCG with deoxyribose sugars but with the normal phosphodiester linker replaced by an N3' --> P5' phosphoramidate (NP) group have been used to establish a solution structure for the duplex. Distance, angle, and base pair hydrogen-bonding constraints were used to ref...

journal_title：Biochemistry

authors： Ding D,Gryaznov SM,Wilson WD

更新日期：1998-09-01 00:00:00

abstract：:One-carbon units are essential to a variety of anabolic processes which yield necessary cellular components including purines, pyrimidines, amino acids, and lipids. Serine hydroxymethyltransferase (SHMT) is the major provider of one-carbon units in the cell. The other product of this reaction is glycine. Both of these...

journal_title：Biochemistry

authors： Kastanos EK,Woldman YY,Appling DR

更新日期：1997-12-02 00:00:00

abstract：:The reduction potentials of the compound II/ferric and compound I/compound II couples have been studied, using potassium hexachloroiridate as a mediator titrant, by thin-layer spectroelectrochemistry. Compound I, which is 2 equiv more oxidized than the ferric (i.e., resting) form of the enzyme, was reversibly formed v...

journal_title：Biochemistry

authors： Farhangrazi ZS,Fossett ME,Powers LS,Ellis WR Jr

更新日期：1995-03-07 00:00:00

abstract：:The mechanism of the oxidative decarboxylation reaction catalyzed by the NAD-malic enzyme from Ascaris suum has been examined with several different divalent metal ion activators and dinucleotide substrates. Primary deuterium and tritium isotope effects have been obtained and, in combination with the partitioning rati...

journal_title：Biochemistry

authors： Karsten WE,Gavva SR,Park SH,Cook PF

更新日期：1995-03-14 00:00:00

abstract：:The phytocyanins are a family of plant cupredoxins that have been subdivided into the stellacyanins, plantacyanins, and uclacyanins. All of these proteins possess the typical type 1 His(2)Cys equatorial ligand set at their mononuclear copper sites, but the stellacyanins have an axial Gln ligand in place of the weakly ...

journal_title：Biochemistry

authors： Harrison MD,Yanagisawa S,Dennison C

更新日期：2005-03-01 00:00:00

abstract：:The N-terminal extracellular parts of human G-protein coupled receptor class B, for example, receptors for secretin, glucagon, or parathyroid hormone, are involved in ligand binding. To obtain structural and functional information on the N-terminal receptor fragment of human parathyroid hormone receptor 1 (PTHR1), the...

journal_title：Biochemistry

authors： Grauschopf U,Lilie H,Honold K,Wozny M,Reusch D,Esswein A,Schäfer W,Rücknagel KP,Rudolph R

更新日期：2000-08-01 00:00:00

abstract：:Under suitable experimental conditions the aerobic oxidation of NADH catalyzed by horseradish peroxidase occurred in four characteristic phases: initial burst, induction phase, steady state, and termination. A trace amount of H2O2 present in the NADH solution brought about initial burst in the formation of oxyperoxida...

journal_title：Biochemistry

authors： Yokota K,Yamazaki I

更新日期：1977-05-03 00:00:00

abstract：:If cytochrome c oxidase is subjected to pressure during the aerobic steady state, large spectral changes are apparent. These seem to be associated with the inhibition of electron transport within the oxidase. The volume change for the transition is about 80 mL/mol. When the oxidase in the aerobic steady state, with po...

journal_title：Biochemistry

authors： Kornblatt JA,Hui Bon Hoa G,Heremans K

更新日期：1988-07-12 00:00:00

abstract：:The enzyme phosphoenolpyruvate carboxykinase (PEPCK) catalyzes the reversible conversion of oxalacetate and GTP to phosphoenolpyruvate (PEP), GDP, and CO2. PEPCK from higher organisms is a monomer, specifically requires GTP or ITP, and uses Mn2+ as the activating cation. Currently, there is no crystal structure of GTP...

journal_title：Biochemistry

authors： Holyoak T,Nowak T

更新日期：2001-09-18 00:00:00

abstract：:The Ndi1 enzyme found in the mitochondrial membrane of Saccharomyces cerevisiae is an NDH-2-type alternative NADH-quinone oxidoreductase. As Ndi1 is expected to be a possible remedy for complex I defects of mammalian mitochondria, a detailed biochemical characterization of the enzyme is needed. To identify the ubiquin...

journal_title：Biochemistry

authors： Murai M,Yamashita T,Senoh M,Mashimo Y,Kataoka M,Kosaka H,Matsuno-Yagi A,Yagi T,Miyoshi H

更新日期：2010-04-06 00:00:00

abstract：:A detailed characterization of the main chain and side chain dynamics in R. capsulatus ferrocytochrome c(2) derived from (2)H NMR relaxation of methyl group resonances is presented. (15)N relaxation measurements confirm earlier results indicating that R. capsulatus ferrocytochrome c(2) exhibits minor rotational anisot...

journal_title：Biochemistry

authors： Flynn PF,Bieber Urbauer RJ,Zhang H,Lee AL,Wand AJ

更新日期：2001-06-05 00:00:00

abstract：:A subgroup of enzymes in the NAD(P)H:FMN reductase family is comprised of flavin reductases from two-component monooxygenase systems. The diverging structural feature in these FMN reductases is a π-helix centrally located at the tetramer interface that is generated by the insertion of an amino acid in a conserved α4 h...

journal_title：Biochemistry

authors： Musila JM,L Forbes D,Ellis HR

更新日期：2018-07-31 00:00:00