Investigation of phosphatidylethanolamine bilayers by deuterium and phosphorus-31 nuclear magnetic resonance.

abstract：:The complete amino acid sequence of a small, basic protein with cardiotoxic activity is described. This toxin, designated Naja naja F8, was isolated from the venom of Naja naja, of Cambodian origin, by gel filtration on Sephadex G-75 followed by gradient ion exchange chromatography on Bio-Rex 70. The cardiotoxin F8, m...

journal_title：Biochemistry

authors： Fryklund L,Eaker D

更新日期：1975-07-01 00:00:00

abstract：:The activation of coagulation factor X by tissue factor (TF) and coagulation factor VIIa (VIIa) on a phospholipid surface is thought to be the key step in the initiation of blood coagulation. In this reaction, the product, fXa, is transiently and reversibly bound to the TF-VIIa enzyme complex. This in effect leads to ...

journal_title：Biochemistry

authors： Hathcock J,Rusinova E,Vaananen H,Nemerson Y

更新日期：2007-05-22 00:00:00

abstract：:The behavior of fluorescent and spin-label probes is examined in several fluid and gel phospholipid phases, with particular focus on the Ca2+-induced gel phase in phosphatidylserine (PS). These probes have behavior characteristic of the type of probe and of the type of lipid environment. Anthroyloxy- and doxyl-labeled...

journal_title：Biochemistry

authors： Florine KI,Feigenson GW

更新日期：1987-03-24 00:00:00

abstract：:We have characterized the importance of size, sulfation, and anticoagulant activity of heparin in release of basic fibroblast growth factor (bFGF) from the subendothelial extracellular matrix (ECM) and the luminal surface of the vascular endothelium. For this purpose, 125I-bFGF was first incubated with ECM and conflue...

journal_title：Biochemistry

authors： Ishai-Michaeli R,Svahn CM,Weber M,Chajek-Shaul T,Korner G,Ekre HP,Vlodavsky I

更新日期：1992-02-25 00:00:00

abstract：:Animal galectins (lectins with specificity for beta-galactosides of glycan chains) are potent effectors in diverse aspects of cell sociology. Gene divergence has led to different groups and a marked interspecies variability in the number of members per group. Since the suitability of a model for studying functionality...

journal_title：Biochemistry

authors： Kaltner H,Solís D,André S,Lensch M,Manning JC,Mürnseer M,Sáiz JL,Gabius HJ

更新日期：2009-05-26 00:00:00

abstract：:The cellular site of formation, Galpha-coupling preference, and agonist regulation of mu-delta opioid receptor (OR) heterooligomers were studied. Bioluminescence resonance energy transfer (BRET) showed that mu-deltaOR heterooligomers, composed of preformed mu and delta homooligomers, interacted constitutively in the e...

journal_title：Biochemistry

authors： Hasbi A,Nguyen T,Fan T,Cheng R,Rashid A,Alijaniaram M,Rasenick MM,O'Dowd BF,George SR

更新日期：2007-11-13 00:00:00

abstract：:Guanine nucleotide dissociation stimulator (GDS) promotes the release of tightly bound GDP from various Ras superfamily proteins, including RhoA, Rac1, K-Ras, Rap1A, and Rap1B. It displays no significant sequence homology to other known exchange factors for small G-proteins. Studies are reported here of the mechanism ...

journal_title：Biochemistry

authors： Hutchinson JP,Eccleston JF

更新日期：2000-09-19 00:00:00

abstract：:We report here on the Raman spectra of NADH, 3-acetylpyridine adenine dinucleotide, APAD+, and a fragment of these molecules, adenosine 5'-diphosphate ribose (ADPR) bound to the mitochondrial (mMDH) and cytoplasmic (or soluble, sMDH) forms of malate dehydrogenase. We observe changes in the Raman spectrum of the adenos...

journal_title：Biochemistry

authors： Deng H,Burgner J,Callender R

更新日期：1991-09-10 00:00:00

abstract：:13C NMR spectroscopy was used to probe the structural interactions between carboxyl-13C-enriched oleic acid (18:1) and rat liver fatty acid binding protein (FABP) and the partitioning of 18:1 between FABP and unilamellar phosphatidylcholine (PC) vesicles. Spectra of systems containing 2-8 mol of 18:1/mol of FABP (but ...

journal_title：Biochemistry

authors： Cistola DP,Walsh MT,Corey RP,Hamilton JA,Brecher P

更新日期：1988-01-26 00:00:00

abstract：:A positional-scanning combinatorial protocol was employed to optimize the deoxyribose-based cleavage of B-form DNA by Ni(II) x Xaa-Xaa-His metallopeptides. This procedure employed 18 naturally occurring amino acids (excluding Cys and Trp) to generate two libraries in which the first and second positions of the peptide...

journal_title：Biochemistry

authors： Huang X,Pieczko ME,Long EC

更新日期：1999-02-16 00:00:00

abstract：:Analogs of EPSP (4-8) have been prepared, and their activity as substrates for the chorismate synthase from Neurospora crassa has been characterized kinetically. The enzyme appears to show strict discrimination against substitution at the Z-position of the enol ether side chain as well as against substitution at the S...

journal_title：Biochemistry

authors： Lauhon CT,Bartlett PA

更新日期：1994-11-29 00:00:00

abstract：:In the globin family, similarities in the folding mechanism have been found among different mammalian apomyoglobins (apoMb). The best-characterized intermediate of sperm whale apoMb, called I(AGH), is mainly stabilized by nativelike contacts among the A, G, and H helices involving a cluster of hydrophobic residues tha...

journal_title：Biochemistry

authors： Musto R,Bigotti MG,Travaglini-Allocatelli C,Brunori M,Cutruzzolà F

更新日期：2004-01-13 00:00:00

abstract：:Bacteriophage T7 RNA polymerase is a single-subunit enzyme which has a C-terminal amino acid sequence of Phe-Ala-Phe-Ala883 (FAFA883). Closely related hydrophobic sequences are present at the C termini of seven other single-subunit RNA polymerases, including the mitochondrial RNA polymerase. Mutations at any of the fo...

journal_title：Biochemistry

authors： Gardner LP,Mookhtiar KA,Coleman JE

更新日期：1997-03-11 00:00:00

abstract：:The specificity of trypsin for Arg- and Lys-containing substrates depends upon the presence of Asp189 at the base of the primary binding pocket. The crystal structure of anionic rat trypsin D189S complexed with BPTI reveals that removal of the aspartate side chain permits the binding of a well-ordered acetate ion in a...

journal_title：Biochemistry

authors： Perona JJ,Hedstrom L,Wagner RL,Rutter WJ,Craik CS,Fletterick RJ

更新日期：1994-03-22 00:00:00

abstract：:Analysis of [gamma-32P]ATP in vitro labeled nuclear RNA shows transfer of gamma-32P from ATP to form the 5'-terminal monophosphate for large RNA molecules. This finding is an actively transcribing nuclear system capable of guanylation and methylation reactions indicates that polynucleotide kinase activity in the eukar...

journal_title：Biochemistry

authors： Winicov I

更新日期：1977-09-20 00:00:00

abstract：:Methyl esterification of carboxylic acid residues in intact mouse S49 lymphoma cells was examined, and at least 24 proteins were found to be modified. Cell fractionation revealed that a distinct set of these proteins could be found in each of the four fractions. Nuclei contained 11 methyl-esterified proteins at 12, 15...

journal_title：Biochemistry

authors： Chelsky D,Ruskin B,Koshland DE Jr

更新日期：1985-11-05 00:00:00

abstract：:The reversible decline of the nicotinic acetylcholine receptor's response to acetylcholine during prolonged exposure to acetylcholine is known as desensitization. Here, we studied ethanol's modulation of fast agonist-induced desensitization of the nicotinic acetylcholine receptor in postsynaptic membrane vesicles from...

journal_title：Biochemistry

authors： Wu G,Miller KW

更新日期：1994-08-09 00:00:00

abstract：:The anaerobic global regulator FNR from Escherichia coli is a [4Fe-4S](2+) cluster-containing dimer that is inactivated by O(2) through disruption of the Fe-S cluster and conversion to the monomeric apoprotein. It was shown that apo-FNR is subject to ClpXP proteolysis, and two recognition sites, amino acids 5-11 and a...

journal_title：Biochemistry

authors： Pan Q,Shan Y,Yan A

更新日期：2012-06-26 00:00:00

abstract：:Aromatic amino acids often flank the transmembrane alpha helices of integral membrane proteins. By favoring locations within the membrane-water interface of the lipid bilayer, aromatic residues Trp, Tyr, and sometimes Phe may serve as anchors to help stabilize a transmembrane orientation. In this work, we compare the ...

journal_title：Biochemistry

authors： Sparks KA,Gleason NJ,Gist R,Langston R,Greathouse DV,Koeppe RE 2nd

更新日期：2014-06-10 00:00:00

abstract：:Photosystem II catalyzes the light-driven oxidation of water and reduction of plastoquinone in oxygenic photosynthesis. The manganese stabilizing protein (MSP) of photosystem II is an extrinsic subunit that plays an important role in catalytic activity. This subunit can be extracted and re-bound to the photosystem II ...

journal_title：Biochemistry

authors： Hutchison RS,Betts SD,Yocum CF,Barry BA

更新日期：1998-04-21 00:00:00

abstract：:Phenylalanine acts as an allosteric activator of the tetrahydropterin-dependent enzyme phenylalanine hydroxylase. Hydrogen/deuterium exchange monitored by mass spectrometry has been used to gain insight into local conformational changes accompanying activation of rat phenylalanine hydroxylase by phenylalanine. Peptide...

journal_title：Biochemistry

authors： Li J,Dangott LJ,Fitzpatrick PF

更新日期：2010-04-20 00:00:00

abstract：:The influenza virus RNA-dependent RNA polymerase protein complex contains an associated RNA endonuclease activity, which cleaves host mRNA precursors in the cell nucleus at defined positions 9-15 nucleotides downstream of the cap structure. This reaction provides capped oligoribonucleotides, which function as primers ...

journal_title：Biochemistry

authors： Doan L,Handa B,Roberts NA,Klumpp K

更新日期：1999-04-27 00:00:00

abstract：:Time-resolved resonance Raman spectroscopy has been employed to monitor geminate heme-CO rebinding in photolyzed HbCO. The excitation frequency was tuned to enhance the scattering from rebound heme sites 20-500 ns subsequent to CO photolysis. The behavior of vFe-C during ligand rebinding has important ramifications co...

journal_title：Biochemistry

authors： Schneebeck MC,Vigil LE,Friedman JM,Chavez MD,Ondrias MR

更新日期：1993-02-09 00:00:00

abstract：:In order to compare the interfacial behavior of the polyheme cytochromes c which belong to the cytochrome c3 superfamily, the monomolecular film technique was used to determine whether and how these metalloproteins interact with (phospho)lipids). Measurements of the variations of surface pressure and surface potential...

journal_title：Biochemistry

authors： Florens L,Ivanova M,Dolla A,Czjzek M,Haser R,Verger R,Bruschi M

更新日期：1995-09-12 00:00:00

abstract：:The reaction of cytochrome c oxidase with dioxygen has been studied by means of time-resolved measurements of electrical membrane potential (DeltaPsi). Microsecond time resolution was achieved by starting with the CO-inhibited enzyme, which was photolyzed after addition of oxygen. The time course of the reaction could...

journal_title：Biochemistry

authors： Jasaitis A,Verkhovsky MI,Morgan JE,Verkhovskaya ML,Wikström M

更新日期：1999-03-02 00:00:00

abstract：:The crystal structure of the catalytic domain of rat DNA polymerase beta revealed that Asp256 is located in proximity to the previously identified active site residues Asp190 and Asp192. We have prepared and kinetically characterized the nucleotidyl transfer activity of wild type and several mutant forms of human and ...

journal_title：Biochemistry

authors： Menge KL,Hostomsky Z,Nodes BR,Hudson GO,Rahmati S,Moomaw EW,Almassy RJ,Hostomska Z

更新日期：1995-12-12 00:00:00

abstract：:Although plasma membrane domains, such as caveolae, provide an organizing principle for signaling pathways and cholesterol homeostasis in the cell, relatively little is known regarding specific mechanisms, whereby intracellular lipid-binding proteins are targeted to caveolae. Therefore, the interaction between caveoli...

journal_title：Biochemistry

authors： Parr RD,Martin GG,Hostetler HA,Schroeder ME,Mir KD,Kier AB,Ball JM,Schroeder F

更新日期：2007-07-17 00:00:00

abstract：:4-Hydroxybenzoyl-CoA reductase (4-HBCR) is a member of the xanthine oxidase (XO) family of molybdenum cofactor containing enzymes and catalyzes the irreversible removal of a phenolic hydroxy group by reduction, yielding benzoyl-CoA and water. In this work the effects of various activity modulating compounds were chara...

journal_title：Biochemistry

authors： Johannes J,Unciuleac MC,Friedrich T,Warkentin E,Ermler U,Boll M

更新日期：2008-04-29 00:00:00

abstract：:DREAM (also known as K(+) channel interacting protein 3 and calsenilin) is a calcium binding protein and an active modulator of KV4 channels in neuronal cells as well as a novel Ca(2+)-regulated transcriptional modulator. DREAM has also been associated with the regulation of Alzheimer's disease through the prevention ...

journal_title：Biochemistry

authors： Gonzalez WG,Ramos V,Diaz M,Garabedian A,Molano-Arevalo JC,Fernandez-Lima F,Miksovska J

更新日期：2016-03-29 00:00:00

abstract：:Transfection of cos-1 cells with either prostaglandin endoperoxide H synthase-1 (PGHS-1) or -2 (PGHS-2) results in a mixed population of cells containing a diverse range of expressed enzyme. The use of fluorescent substrates and antibodies, in conjunction with fluorescence microscopy, provides the means to quantitate ...

journal_title：Biochemistry

authors： Morita I,Smith WL,DeWitt DL,Schindler M

更新日期：1995-05-30 00:00:00