Inhibition of tubulin self-assembly and tubulin-colchicine GTPase activity by guanosine 5'-(gamma-fluorotriphosphate).

abstract：:The hypothesis that the stable ternary complex formed between myosin subfragment-1, MgADP and beryllium fluoride (BeF3-), denoted S-1 not equal to .ADP.BeF3-, is an analog of the intermediate state S-1**.ADP.P(i) has been tested in this work by examining the interactions of S-1 not equal to .ADP.BeF3- with actin. Equi...

journal_title：Biochemistry

authors： Phan BC,Faller LD,Reisler E

更新日期：1993-08-03 00:00:00

abstract：:We have developed a system for targeting foreign DNA to hepatocytes in vitro using a soluble DNA carrier that takes advantage of receptor-mediated endocytosis to achieve internalization. The idea is based on the fact that hepatocytes possess a unique receptor that binds and internalizes galactose-terminal (asialo)glyc...

journal_title：Biochemistry

authors： Wu GY,Wu CH

更新日期：1988-02-09 00:00:00

abstract：:Some key chloroplast enzymes are activated by light via a ferredoxin-thioredoxin reduction system which reduces disulfide bridges in the enzymes. We describe for the first time the structural basis for the redox activation of a chloroplast enzyme, the NADP-dependent malate dehydrogenase (MDH) from Sorghum vulgare whos...

journal_title：Biochemistry

authors： Johansson K,Ramaswamy S,Saarinen M,Lemaire-Chamley M,Issakidis-Bourguet E,Miginiac-Maslow M,Eklund H

更新日期：1999-04-06 00:00:00

abstract：:Budding of retroviruses requires the structural precursor polyprotein, Gag, to target the plasma membrane through its N-terminal matrix (MA) domain. For HIV-1, the interaction between membrane signaling molecule phosphatidylinositol 4,5-diphosphate (PIP2) and MA induces the exposure of myristate and promotes membrane ...

journal_title：Biochemistry

authors： Chen K,Bachtiar I,Piszczek G,Bouamr F,Carter C,Tjandra N

更新日期：2008-02-19 00:00:00

abstract：:Hydroxyproline-rich glycoproteins (HRGPs) are the major proteinaceous components of higher plant walls and the predominant components of the cell wall of the green alga Chlamydomonas reinhardtii. The GP1 protein, an HRGP of the C. reinhardtii wall, is shown to adopt a polyproline II helical configuration and to carry ...

journal_title：Biochemistry

authors： Ferris PJ,Woessner JP,Waffenschmidt S,Kilz S,Drees J,Goodenough UW

更新日期：2001-03-06 00:00:00

abstract：:Two terminally blocked peptides, acetylAETAAAKFLRQHMamide and acetylAETSSSRYLRQHMamide, were obtained by solid-phase synthesis, purified by reversed-phase chromatography, and characterized by fast atom bombardment mass spectrometry. Both peptides were soluble in aqueous solutions and remained monomeric over the concen...

journal_title：Biochemistry

authors： Merutka G,Stellwagen E

更新日期：1989-01-10 00:00:00

abstract：:The concentration of hyaluronic acid, chondroitin sulfate, and heparan sulfate was measured in rat brain at 2-day intervals from birth to 1 month of age, and in 40-day-old and adult animals. The levels of all three glycosaminoglycans increased after birth to reach a peak at 7 days after which they declined steadily, a...

journal_title：Biochemistry

authors： Margolis RU,Margolis RK,Chang LB,Preti C

更新日期：1975-01-14 00:00:00

abstract：:Recently we demonstrated that the unique stereoelectronic relationships inherent in the structure of plasmenylethanolamine facilitate membrane fusion, and we postulated the existence of a membrane fusion protein which could exploit the propensity of plasmenylethanolamine molecular species to adapt an inverted hexagona...

journal_title：Biochemistry

authors： Glaser PE,Gross RW

更新日期：1995-09-26 00:00:00

abstract：:Classical molecular dynamics simulations were utilized to investigate the structural and dynamical properties of water in the active site of ketosteroid isomerase (KSI) to provide insight into the role of these water molecules in the enzyme-catalyzed reaction. This reaction is thought to proceed via a dienolate interm...

journal_title：Biochemistry

authors： Hanoian P,Hammes-Schiffer S

更新日期：2011-08-09 00:00:00

abstract：:Polyisoprenyl diphosphates play diverse and vital roles in cell function in health and disease. The counter-regulatory lipid signaling molecule, presqualene diphosphate (PSDP), is rapidly converted to its monophosphate form (PSMP) upon cell activation [Levy, B. D., Petasis, N. A., and Serhan, C. N. (1997) Nature 389, ...

journal_title：Biochemistry

authors： Carlo T,Petasis NA,Levy BD

更新日期：2009-04-07 00:00:00

abstract：:A role for Tyr254 in L-lactate dehydrogenation catalyzed by flavocytochrome b2 has recently been proposed on the basis of the known active-site structure and of studies that had suggested a mechanism involving the initial formation of a lactate carbanion [Lederer, F., & Mathews, F.S. (1987) in Flavins and Flavoprotein...

journal_title：Biochemistry

authors： Dubois J,Chapman SK,Mathews FS,Reid GA,Lederer F

更新日期：1990-07-10 00:00:00

abstract：:Beta(2)-microglobulin (beta(2)m) forms amyloid fibrils that deposit in the musculo-skeletal system in patients undergoing long-term hemodialysis. How beta(2)m self-assembles in vivo is not understood, since the monomeric wild-type protein is incapable of forming fibrils in isolation in vitro at neutral pH, while elong...

journal_title：Biochemistry

authors： Myers SL,Jones S,Jahn TR,Morten IJ,Tennent GA,Hewitt EW,Radford SE

更新日期：2006-02-21 00:00:00

abstract：:Compartmentation and NMR visibility of mitochondrial adenine nucleotides were quantitated in isolated rat liver mitochondria respiring on succinate and glutamate in vitro at 8 and 25 degrees C. Intra- and extramitochondrial nucleotides were discriminated by adding the chelator trans-1,2-diaminocyclohexane-N,N,N',N'-te...

journal_title：Biochemistry

authors： Hutson SM,Berkich D,Williams GD,LaNoue KF,Briggs RW

更新日期：1989-05-16 00:00:00

abstract：:Metabolomics is a rapidly growing field of research used in the identification and quantification of the small molecule metabolites within an organism, thereby providing insights into cell metabolism and bioenergetics as well as processes important in clinical medicine, such as disposition of pharmaceutical compounds....

journal_title：Biochemistry

authors： Milne SB,Mathews TP,Myers DS,Ivanova PT,Brown HA

更新日期：2013-06-04 00:00:00

abstract：:We have purified and characterized a novel 30-kDa glycoprotein (gp30) with TGF alpha-like properties secreted from the estrogen receptor negative breast cancer cell line MDA-MB-231. This factor was immunoprecipitated by an anti-TGF alpha polyclonal antibody and also had TGF alpha-like biological activity, as assayed b...

journal_title：Biochemistry

authors： Lupu R,Wellstein A,Sheridan J,Ennis BW,Zugmaier G,Katz D,Lippman ME,Dickson RB

更新日期：1992-08-18 00:00:00

abstract：:Mutation of polycystin-1 (PC1) is the major cause of autosomal dominant polycystic kidney disease. PC1 has a predicted molecular mass of ~460 kDa comprising a long multidomain extracellular N-terminal region, 11 transmembrane regions, and a short C-terminal region. Because of its size, PC1 has proven difficult to hand...

journal_title：Biochemistry

authors： Oatley P,Stewart AP,Sandford R,Edwardson JM

更新日期：2012-04-03 00:00:00

abstract：:The apolipoprotein apoE plays a key role in cholesterol and lipid metabolism. There are three isoforms of this protein, one of which, apoE4, is the major risk factor for Alzheimer's disease. At micromolar concentrations all lipid-free apoE isoforms exist primarily as monomers, dimers, and tetramers. However, the molec...

journal_title：Biochemistry

authors： Garai K,Baban B,Frieden C

更新日期：2011-04-05 00:00:00

abstract：:The homologous series of optically active short-chain phosphatidylethanolamines (PE) from dibutyryl-PE to dioctanoyl-PE was synthesized. In addition, two monomeric short-chain phospholipid analogues that are not degraded by phospholipase A2 (1,2-bis[(butylcarbamyl)oxy]-sn-glycero-3-phosphocholine and the corresponding...

journal_title：Biochemistry

authors： Plückthun A,Rohlfs R,Davidson FF,Dennis EA

更新日期：1985-07-16 00:00:00

abstract：:New isoforms of CYP51 (sterol 14alpha-demethylase), an essential enzyme in sterol biosynthesis and primary target of azole antimycotic drugs, are found in pathogenic protists, Trypanosoma brucei(TB), T. vivax, T. cruzi, and Leishmania major. The sequences share approximately 80% amino acid identity and are approximate...

journal_title：Biochemistry

authors： Lepesheva GI,Nes WD,Zhou W,Hill GC,Waterman MR

更新日期：2004-08-24 00:00:00

abstract：:The interactions of Hoechst 33377 (H1) with 20 different oligomeric duplexes have been investigated via spectrofluorometric titrations and/or thermal denaturation experiments. H1 is shown to form 2:1 complexes with dsDNA binding sites of at least four contiguous A/T base pairs. H1 is also shown to possess the rare abi...

journal_title：Biochemistry

authors： Satz AL,White CM,Beerman TA,Bruice TC

更新日期：2001-05-29 00:00:00

abstract：:Coiled coils (CCs) are among the best-understood protein folds. Nonetheless, there are gaps in our knowledge of CCs. Notably, CCs are likely to be structurally more dynamic than often considered. Here, we explore this in an abundant class of CCs, parallel dimers, focusing on polar asparagine (Asn) residues in the hydr...

journal_title：Biochemistry

authors： Thomas F,Niitsu A,Oregioni A,Bartlett GJ,Woolfson DN

更新日期：2017-12-19 00:00:00

abstract：:Dehaloperoxidase (DHP), discovered in the marine terebellid polychaete Amphitrite ornata, is the first heme-containing globin with a peroxidase activity. The sequence and crystal structure of DHP argue that it evolved from an ancient O(2) transport and storage globin. Thus, DHP retains an oxygen carrier function but a...

journal_title：Biochemistry

authors： Du J,Huang X,Sun S,Wang C,Lebioda L,Dawson JH

更新日期：2011-09-27 00:00:00

abstract：:Heme oxygenase (HO), from the pathogenic bacterium N. meningitidis(NmHO), which secures host iron, shares many properties with mammalian HOs but also exhibits some key differences. The crystal structure appears more compact, and the crystal-undetected C-terminus interacts with substrate in solution. The unique nature ...

journal_title：Biochemistry

authors： Peng D,Satterlee JD,Ma LH,Dallas JL,Smith KM,Zhang X,Sato M,La Mar GN

更新日期：2011-10-18 00:00:00

abstract：:Proteins performing multiple biochemical functions are called "moonlighting proteins" or extreme multifunctional (EMF) proteins. Mitochondrial cytochrome c is an EMF protein that binds multiple partner proteins to act as a signaling molecule, transfers electrons in the respiratory chain, and acts as a peroxidase in ap...

journal_title：Biochemistry

authors： Deacon OM,Karsisiotis AI,Moreno-Chicano T,Hough MA,Macdonald C,Blumenschein TMA,Wilson MT,Moore GR,Worrall JAR

更新日期：2017-11-21 00:00:00

abstract：:The stoichiometry of cytochrome b559 (one or two copies) per reaction center of photosystem II (PSII) has been the subject of considerable debate. The molar ratio of cytochrome b559 has a number of significant implications on our understanding of the functional role of cytochrome b559, the mechanism of electron donati...

journal_title：Biochemistry

authors： Buser CA,Diner BA,Brudvig GW

更新日期：1992-11-24 00:00:00

abstract：:Structural and genetic studies indicate that the antibacterial compound triclosan, an additive in many personal care products, is an inhibitor of EnvM, the enoyl reductase from Escherichia coli. Here we show that triclosan specifically inhibits InhA, the enoyl reductase from Mycobacterium tuberculosis and a target for...

journal_title：Biochemistry

authors： Parikh SL,Xiao G,Tonge PJ

更新日期：2000-07-04 00:00:00

abstract：:The X-ray structures of the ligand free (apo) and the Mg(2+)*o-succinylbenzoate (OSB) product complex of o-succinylbenzoate synthase (OSBS) from Escherichia coli have been solved to 1.65 and 1.77 A resolution, respectively. The structure of apo OSBS was solved by multiple isomorphous replacement in space group P2(1)2(...

journal_title：Biochemistry

authors： Thompson TB,Garrett JB,Taylor EA,Meganathan R,Gerlt JA,Rayment I

更新日期：2000-09-05 00:00:00

abstract：:The heptadecapeptides bombolitin I and bombolitin III are two of a series of peptides postulated to be biologically active within a membrane environment. In the preceding paper [Bairaktari, E., Mierke, D.F., Mammi, S., & Peggion, E. (1990) Biochemistry (preceding paper in this issue)] the conformational preferences of...

journal_title：Biochemistry

authors： Bairaktari E,Mierke DF,Mammi S,Peggion E

更新日期：1990-10-30 00:00:00

abstract：:H1 can rearrange in chicken erythrocyte polynucleosomes in 80 mM NaCl buffers. These rearrangements have been studied by sedimentation analysis. H1 redistributes between polynucleosomes as well as between polynucleosomes and monosomes. In these rearrangements H1 molecules move to free DNA sites. In contrast to H1, the...

journal_title：Biochemistry

authors： Lasters I,Muyldermans S,Wyns L,Hamers R

更新日期：1981-03-03 00:00:00

abstract：:An amplified Okayama-Berg plasmid cDNA library was constructed from total poly(A)+ RNA isolated from the Madin-Darby bovine kidney cell line MDBK. This library was screened with a partial murine calpactin I heavy chain (p36) cDNA clone, the identification of which was based on bovine p36 tryptic peptide sequences gene...

journal_title：Biochemistry

authors： Kristensen T,Saris CJ,Hunter T,Hicks LJ,Noonan DJ,Glenney JR Jr,Tack BF

更新日期：1986-08-12 00:00:00