Kinetic and equilibrium analysis of the interactions of actomyosin subfragment-1.ADP with beryllium fluoride.

abstract：:It has been suggested that the down-regulation of hepatic HMG-CoA reductase by dietary cholesterol requires modification of the cholesterol molecule before it can exert its suppressive action. In a recent study [Lund, E., Breuer, O., & Björkhem, I. (1992) J. Biol. Chem. 267, 25092-25097], we showed that side-chain hyd...

journal_title：Biochemistry

authors： Lund E,Björkhem I

更新日期：1994-01-11 00:00:00

abstract：:A new strategy for the assignment of nonexchangeable proton resonances in oligonucleotide duplexes is presented and used to interpret the spectra of the oligonucleotide helix d(CpGpCpGpCpG) X d(CpGpCpGpCpG) in low salt (B form). This procedure is based on the use of sequential homodecoupling (1D) or COSY (2D) for the ...

journal_title：Biochemistry

authors： Frechet D,Cheng DM,Kan LS,Ts'o PO

更新日期：1983-10-25 00:00:00

abstract：:We have studied the effect of the binding of ribosomal protein S1 and initiation factor IF3 on the accessibility of nucleotide residues 584-1506 in the small subunit of the Escherichia coli ribosome. Protein S1 strongly decreases RNase V1 attack at G1164, in hairpin 40 of the 3' major domain, and weakly decreases DMS ...

journal_title：Biochemistry

authors： Laughrea M,Tam J

更新日期：1991-12-03 00:00:00

abstract：:Recent crystallographic data suggest that conserved residues in the connection subdomain and C-terminal ribonuclease H (RNase H) domain of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) contact the nascent DNA primer and modulate the trajectory of the template relative to the RNase H catalytic ce...

journal_title：Biochemistry

authors： Rausch JW,Lener D,Miller JT,Julias JG,Hughes SH,Le Grice SF

更新日期：2002-04-16 00:00:00

abstract：:The effect of opportunistic infections (OI) on immune-compromised populations has been known for decades, but the recent AIDS epidemic has sparked renewed interest in the development of new anti-OI agents. The mechanism of action of a series of cationic unfused-aromatic anti-OI drugs is believed to involve binding of ...

journal_title：Biochemistry

authors： Tanious FA,Ding D,Patrick DA,Tidwell RR,Wilson WD

更新日期：1997-12-09 00:00:00

abstract：:Two tetrapeptide sequence homologies between mouse epidermal growth factor precursor (mEGFP) and human follitropin (FSH) were revealed by a computer program that identifies identical residues among polypeptide sequences. The two tetrapeptides, Lys-Thr-Cys-Thr (KTCT) and Thr-Arg-Asp-Leu (TRDL), are present in the hormo...

journal_title：Biochemistry

authors： Sluss PM,Krystek SR Jr,Andersen TT,Melson BE,Huston JS,Ridge R,Reichert LE Jr

更新日期：1986-05-06 00:00:00

abstract：:There are highly conserved hydrogen bonds between the distal His and the adjacent Asn in many peroxidases. Although the crystal structure of horseradish peroxidase C (HRP) is not available, comparison of the amino acid sequence with cytochrome c peroxidase indicates that Asn70 is making the hydrogen bond with the dist...

journal_title：Biochemistry

authors： Nagano S,Tanaka M,Ishimori K,Watanabe Y,Morishima I

更新日期：1996-11-12 00:00:00

abstract：:We have examined in detail the kinetics of binding of the serpin alpha 2-antiplasmin to the serine proteases alpha-chymotrypsin and plasmin. These represent model systems for serpin binding. We find, in contrast to earlier published results with alpha 2-antiplasmin and plasmin, that binding is reversible, and slow bin...

journal_title：Biochemistry

authors： Longstaff C,Gaffney PJ

更新日期：1991-01-29 00:00:00

abstract：:The pathogenic bacteria Pseudomonas aeruginosa and Bordetella pertussis contain in their outer membranes the rare sugar 2,3-diacetamido-2,3-dideoxy-d-mannuronic acid. Five enzymes are required for the biosynthesis of this sugar starting from UDP-N-acetylglucosamine. One of these, referred to as WlbB, is an N-acetyltra...

journal_title：Biochemistry

authors： Thoden JB,Holden HM

更新日期：2010-06-08 00:00:00

abstract：:The structure and folding of a novel human insulin mutant, [Thr(B27) --> Pro, Pro(B28) --> Thr]insulin (PT insulin), in aqueous solution and in mixtures of water and 2,2,2-trifluoroethanol (TFE) have been studied by NMR spectroscopy. It was found that PT insulin has a highly flexible structure in pure water and is pre...

journal_title：Biochemistry

authors： Keller D,Clausen R,Josefsen K,Led JJ

更新日期：2001-09-04 00:00:00

abstract：:We determined the effect of vertebrate albumins on the stability of several physiologically relevant prostaglandins. All naturally occurring prostaglandins with beta-hydroxy ketone group decomposed by first-order kinetics, dependent on the albumin concentration in 0.1 M, pH 7.4, buffer at 37 degrees C. Even subphysiol...

journal_title：Biochemistry

authors： Fitzpatrick FA,Waynalda MA

更新日期：1981-10-13 00:00:00

abstract：:While the urea-mediated unfolding pathway of the Escherichia coli aspartate aminotransferase (eAATase) homodimer proceeds through a reversible three-state process with a partially folded dimeric intermediate, D D* 2U (E. Deu and J. F. Kirsch, accompanying paper), that of a cofactor-stabilized form differs. Pyridoxal p...

journal_title：Biochemistry

authors： Deu E,Kirsch JF

更新日期：2007-05-15 00:00:00

abstract：:The interplay between the early collapse of the unfolded state and the formation of the secondary structure has been the subject of extensive research in protein chemistry. In this study, we used the intestinal fatty acid binding protein (IFABP), a small model protein with predominately β-sheet structure, to study the...

journal_title：Biochemistry

authors： Sarkar S,Chattopadhyay K

更新日期：2014-03-11 00:00:00

abstract：:Backbone amide dynamics of the Escherichia coli tryptophan repressor protein (WT-TrpR) and two functionally distinct variants, L75F-TrpR and A77V-TrpR, in their holo (l-tryptophan corepressor-bound) form have been characterized using (15)N nuclear magnetic resonance (NMR) relaxation. The three proteins possess very si...

journal_title：Biochemistry

authors： Tripet BP,Goel A,Copie V

更新日期：2011-06-14 00:00:00

abstract：:Two photoreactive apamin derivatives were prepared with an aryl azide [[(azidonitrophenyl)amino]acetate (ANPAA)] group coupled at different positions on the neurotoxin molecule. These ligands were used to identify membrane components in the environment of the neuronal binding site that is associated with a Ca2+-activa...

journal_title：Biochemistry

authors： Seagar MJ,Labbé-Jullié C,Granier C,Goll A,Glossmann H,Van Rietschoten J,Couraud F

更新日期：1986-07-15 00:00:00

abstract：:NG-Methyl-L-arginine (L-NMA) is one of the most commonly used inhibitors of the nitric oxide synthases (NOS). Results reported here demonstrate that L-NMA is an alternate substrate and a mechanism-based inhibitor of the inducible NOS purified from murine macrophages. The irreversible inhibition displays pseudo-first-o...

journal_title：Biochemistry

authors： Olken NM,Marletta MA

更新日期：1993-09-21 00:00:00

abstract：:3-(Trifluoromethyl)-3-(m-iodophenyl)diazirine (TID) has recently been found to be a noncompetitive inhibitor of the nicotinic acetylcholine receptor (nAcChoR) by both photolabeling and flux assays (White et al., 1991). However, these experiments were done when TID was in equilibrium with the nAcChoR, and thus only its...

journal_title：Biochemistry

authors： Wu G,Raines DE,Miller KW

更新日期：1994-12-27 00:00:00

abstract：:The genome of all retroviruses consists of two identical RNAs noncovalently linked near their 5' end. Dimerization of genomic RNA is thought to modulate several steps in the retroviral life cycle, such as recombination, translation, and encapsidation. The kissing-loop model of HIV-1 genome dimerization [Laughrea, M., ...

journal_title：Biochemistry

authors： Laughrea M,Jetté L

更新日期：1996-02-06 00:00:00

abstract：:Phase-sensitive 2D 1H/1H COSY spectra can be used to identify the structures of individual pure specimens of the aminoglycoside antibiotic amikacin and its N-hemisuccinyl derivatives. However, even at 500 MHz the 2D chemical shift dispersion does not allow for unambiguous assignment of all cross-peaks. By use of 2D re...

journal_title：Biochemistry

authors： Andersen NH,Eaton HL,Nguyen KT,Hartzell C,Nelson RJ,Priest JH

更新日期：1988-04-19 00:00:00

abstract：:The acid-induced arginine decarboxylase is part of an enzymatic system in Escherichia coli that contributes to making this organism acid resistant. The arginine decarboxylase is a vitamin B(6)-dependent enzyme that is active at acidic pH. It consumes a proton in the decarboxylation of arginine to agmatine, and by work...

journal_title：Biochemistry

authors： Andréll J,Hicks MG,Palmer T,Carpenter EP,Iwata S,Maher MJ

更新日期：2009-05-12 00:00:00

abstract：:Transition metal-transporting P1B-type CPx ATPases play crucial roles in mediating metal homeostasis and resistance in all cells. The degree to which N-terminal metal binding domains (MBDs) confer metal specificity to the transporter is unclear. We show that the two MBDs of the Zn/Cd/Pb effluxing pump Anabaena AztA ar...

journal_title：Biochemistry

authors： Liu T,Reyes-Caballero H,Li C,Scott RA,Giedroc DP

更新日期：2007-10-02 00:00:00

abstract：:The transcriptional activity of the hexafluorinated derivative of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], 26,26,26,27,27,27-hexafluoro-1,25-dihydroxyvitamin D3 [F6-1,25-(OH)2D3], was examined in cultured cells by a transient expression assay (CAT assay) using expression vectors for the rat nuclear vitamin D3 receptor...

journal_title：Biochemistry

authors： Sasaki H,Harada H,Handa Y,Morino H,Suzawa M,Shimpo E,Katsumata T,Masuhiro Y,Matsuda K,Ebihara K

更新日期：1995-01-10 00:00:00

abstract：:The effect of low molecular-weight compounds on the equilibrium constant K(A) can be used to explore the energetics and molecular mechanism of protein-DNA interactions. Here we use the complex composed of the integrase Tn916 DNA-binding domain and its target DNA duplex to investigate the effects of salt and the nonion...

journal_title：Biochemistry

authors： Milev S,Bosshard HR,Jelesarov I

更新日期：2005-01-11 00:00:00

abstract：:Thermobifida fusca Cel9A-90, an unusual family 9 enzyme, is a processive endoglucanase containing a catalytic domain closely linked to a family 3c cellulose binding domain (Cel9A-68) followed by a fibronectin III-like domain and a family 2 cellulose binding domain. To study its catalytic mechanism, 12 mutant genes wit...

journal_title：Biochemistry

authors： Zhou W,Irwin DC,Escovar-Kousen J,Wilson DB

更新日期：2004-08-03 00:00:00

abstract：:The synthesis is described of four new cleavable, photosensitive, heterobifunctional cross-linking reagents for use in examining membrane organization: 4'-azidoazobenzene-4oxysuccinimide ester (1), N-[4-(p-azidophenylazo)benzoyl]-3-aminopropyl-N'-oxysuccinimide ester (2), N-[4-(p-azidophenylazo)benzoyl]-6-aminohexyl-N...

journal_title：Biochemistry

authors： Jaffe CL,Lis H,Sharon N

更新日期：1980-09-16 00:00:00

abstract：:The synthesis of activated sulfate (adenosine 5'-phosphosulfate, APS) and inorganic pyrophosphate from ATP and SO4 is remarkably unfavorable: K(eq) approximately 10(-8) under presumed, near-physiological conditions. Consequently, ATP sulfurylases, which catalyze APS synthesis, suffer approximately 10(8)-fold losses in...

journal_title：Biochemistry

authors： Sun M,Leyh TS

更新日期：2006-09-26 00:00:00

abstract：:The mechanism of chromophore biosynthesis in green fluorescent protein (GFP) is triggered by a spontaneous main chain cyclization reaction of residues 65-67. Here, we demonstrate that the initially colorless Y66L variant, designed to trap chromophore precursor states, is oxidatively modified to generate yellow chromop...

journal_title：Biochemistry

authors： Rosenow MA,Patel HN,Wachter RM

更新日期：2005-06-14 00:00:00

abstract：:Studies of intragenomic homologues in bacterial genomes can provide valuable insights into functional divergence. Three GTP cyclohydrolase II homologues in the Streptomyces coelicolor genome have been shown to catalyze two related but distinct reactions [Spoonamore, J. E., Dahlgran, A. L., Jacobsen, N. E., and Bandari...

journal_title：Biochemistry

authors： Spoonamore JE,Bandarian V

更新日期：2008-02-26 00:00:00

abstract：:Sedimentation in a preparative ultracentrifuge was used to determine the affinity of heavy meromyosin, HMM, for regulated actin, F-actin plus troponin-tropomyosin, in the presence of MgATP at pH 7.0, 20 degrees C, and mu = 18 mM. HMM was prepared from vertebrate striated muscle myosin by a mild chymotryptic digestion....

journal_title：Biochemistry

authors： Wagner PD,Stone DB

更新日期：1983-03-15 00:00:00

abstract：:Sendai virus particles are able to fuse with Pronase-neuraminidase-treated human erythrocyte membranes as well as with vesicles obtained from chromaffin granules of bovine medulla. Fusion is inferred either from electron microscopic studies or from the observation that incubation of fluorescently labeled (bearing octa...

journal_title：Biochemistry

authors： Citovsky V,Laster Y,Schuldiner S,Loyter A

更新日期：1987-06-30 00:00:00