Substrate specificity and excision kinetics of Escherichia coli endonuclease VIII (Nei) for modified bases in DNA damaged by free radicals.

abstract：:The N-terminal formamido group on imidazole- and pyrrole-containing polyamides causes stacked polyamides to bind in the minor groove of DNA in the staggered motif, and it also increases the binding affinity compared to those of non-formamido compounds. To further investigate the role of the N-terminal acylamido in aff...

journal_title：Biochemistry

authors： Westrate L,Mackay H,Brown T,Nguyen B,Kluza J,Wilson WD,Lee M,Hartley JA

更新日期：2009-06-23 00:00:00

abstract：:Aquaporin-4 (AQP4) is a water channel found at high concentrations around blood vessels in the brain and is organized into elaborate assemblies called square arrays. The natural functions of AQP4 and the square arrays remain unknown, but under pathophysiological conditions, AQP4 has been shown to influence brain edema...

journal_title：Biochemistry

authors： Strand L,Moe SE,Solbu TT,Vaadal M,Holen T

更新日期：2009-06-30 00:00:00

abstract：:Protein kinase Calpha (PKCalpha) has been shown to contain two discrete activator sites with differing binding affinities for phorbol esters and diacylglycerols. The interaction of diacylglycerol with a low-affinity phorbol ester binding site leads to enhanced high-affinity phorbol ester binding and to a potentiated l...

journal_title：Biochemistry

authors： Slater SJ,Milano SK,Stagliano BA,Gergich KJ,Ho C,Mazurek A,Taddeo FJ,Kelly MB,Yeager MD,Stubbs CD

更新日期：1999-03-23 00:00:00

abstract：:Double strand breaks in DNA can be quantified down to very low frequencies (a few per Gigabase pair) in nanogram quantities of nonradioactive, genomic DNA by dispersing the DNAs on electrophoretic gels, digitizing them by quantitative electronic imaging, and calculating the DNA lengths by number average length analysi...

journal_title：Biochemistry

authors： Sutherland BM,Bennett PV,Georgakilas AG,Sutherland JC

更新日期：2003-03-25 00:00:00

abstract：:G proteins play an important role in transmitting hormonal signals, and fluorescence techniques would be useful to study their cellular distribution and mechanisms. To prepare active fluorescent G protein Go/Gi or beta gamma subunits were reacted with fluorescein isothiocyanate (FITC) to label the alpha (F-alpha) and ...

journal_title：Biochemistry

authors： Kwon G,Remmers AE,Datta S,Neubig RR

更新日期：1993-03-09 00:00:00

abstract：:The bacterial phosphotriesterase (PTE) from Pseudomonas diminuta catalyzes the hydrolysis of organophosphate esters at rates close to the diffusion limit. X-ray diffraction studies have shown that a binuclear metal center is positioned in the active site of PTE and that this complex is responsible for the activation o...

journal_title：Biochemistry

authors： Kim J,Tsai PC,Chen SL,Himo F,Almo SC,Raushel FM

更新日期：2008-09-09 00:00:00

abstract：:In this work, we show that adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S) is a substrate for yeast inorganic pyrophosphatase (PPase) (EC 3.6.1.1) and further, using chirally labeled [gamma-17O,18O]ATP gamma S, that enzyme-catalyzed hydrolysis to produce chiral inorganic thio[17O,18O]phosphate proceeds with inversio...

journal_title：Biochemistry

authors： Gonzalez MA,Webb MR,Welsh KM,Cooperman BS

更新日期：1984-02-28 00:00:00

abstract：:The enzyme As(III) S-adenosylmethionine methyltransferase (EC 2.1.1.137) (ArsM or AS3MT) is found in members of every kingdom, from bacteria to humans. In these enzymes, there are three conserved cysteine residues at positions 72, 174, and 224 in the CmArsM orthologue from the thermophilic eukaryotic alga Cyanidioschy...

journal_title：Biochemistry

authors： Marapakala K,Qin J,Rosen BP

更新日期：2012-02-07 00:00:00

abstract：:This paper defines the properties of interaction of endothelin-1 (Et-1) with cloned bovine ETA receptors. The Kd value of Et-1/ETA receptor complexes was estimated in membrane preparations to 20 pM using kinetic experiments and saturation experiments performed under quasi equilibrium conditions. Competition experiment...

journal_title：Biochemistry

authors： Desmarets J,Gresser O,Guedin D,Frelin C

更新日期：1996-11-26 00:00:00

abstract：:The class I human liver alcohol dehydrogenases (ADHs) catalyze the interconversion of the intermediary alcohols and aldehydes of dopamine metabolism in vitro, whereas those of the class II and class III do not. The individual, homogeneous class I isozymes oxidize (3,4-dihydroxyphenyl)ethanol and (4-hydroxy-3-methoxyph...

journal_title：Biochemistry

authors： Mårdh G,Vallee BL

更新日期：1986-11-18 00:00:00

abstract：:alpha-Conotoxin GI is a snail toxin protein consisting of 13 amino acids cross-linked by 2 intramolecular disulfide bridges. This toxin is an antagonist of acetylcholine receptors. The native sequence has been synthesized, along with nine additional variants in which non-cysteine residues are replaced by alanine or th...

journal_title：Biochemistry

authors： Zhang RM,Snyder GH

更新日期：1991-11-26 00:00:00

abstract：:Fibronectin (FN) is an extracellular matrix (ECM) protein found soluble in corporal fluids or as an insoluble fibrillar component incorporated in the ECM. This phenomenon implicates structural changes that expose FN binding sites and activate the protein to promote intermolecular interactions with other FN. We have in...

journal_title：Biochemistry

authors： Patel S,Chaffotte AF,Goubard F,Pauthe E

更新日期：2004-02-17 00:00:00

abstract：:The heme propionate groups of both myoglobin (Mb) and cytochrome b5 form hydrogen bonds with nearby surface amino acids residues that are believed to stabilize the heme-protein complex. To evaluate the magnitude of this stabilization, the kinetics of heme dissociation from variants of horse heart Mb and cytochrome b5 ...

journal_title：Biochemistry

authors： Hunter CL,Lloyd E,Eltis LD,Rafferty SP,Lee H,Smith M,Mauk AG

更新日期：1997-02-04 00:00:00

abstract：:The Raman laser temperature-jump technique has been used to measure the kinetics of the coil to helix reaction of poly(cytidylic acid) [poly(C)] in aqueous cosolvent mixtures. The rate of helix formation has a low activation energy and is proportional to reciprocal solvent viscosity. The observations suggest helix for...

journal_title：Biochemistry

authors： Freier SM,Hill KO,Dewey TG,Marky LA,Breslauer KJ,Turner DH

更新日期：1981-03-17 00:00:00

abstract：:Guanine nucleotide dissociation stimulator (GDS) promotes the release of tightly bound GDP from various Ras superfamily proteins, including RhoA, Rac1, K-Ras, Rap1A, and Rap1B. It displays no significant sequence homology to other known exchange factors for small G-proteins. Studies are reported here of the mechanism ...

journal_title：Biochemistry

authors： Hutchinson JP,Eccleston JF

更新日期：2000-09-19 00:00:00

abstract：:The far-ultraviolet circular dichroism spectra of bovine and human prothrombin, prothrombin fragment 1, prethrombin 1, prothrombin fragment 2, and prethrombin 2 (prethrombin 2des(1--13)) were determined and the method of Chen et al. [Chen, Y. H., Yang, J. T., & Martinez, H. M. (1972) Biochemistry 11, 4120--4131; Chen,...

journal_title：Biochemistry

authors： Bloom JW,Mann KG

更新日期：1979-05-15 00:00:00

abstract：:The continued rise of antibiotic-resistant infections coupled with the limited pipeline of new antimicrobials highlights the pressing need for the development of new antibacterial agents. One potential pathway for new agents is de novo purine biosynthesis as studies have shown that bacteria and lower eukaryotes synthe...

journal_title：Biochemistry

authors： Streeter CC,Lin Q,Firestine SM

更新日期：2019-04-30 00:00:00

abstract：:Deuterium NMR has been used to investigate the structure and dynamic state of cytochrome c complexed with bilayers of cardiolipin. Reductive methylation was employed to prepare [N epsilon, N epsilon-C2H3]lysyl cytochrome c, and deuterium exchange provided labeling of backbone sites to give [amide-2H]cytochrome c or mo...

journal_title：Biochemistry

authors： Spooner PJ,Watts A

更新日期：1991-04-23 00:00:00

abstract：:β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the induction of resistance. We report s...

journal_title：Biochemistry

authors： Blázquez B,Llarrull LI,Luque-Ortega JR,Alfonso C,Boggess B,Mobashery S

更新日期：2014-03-18 00:00:00

abstract：:Pig pancreatic alpha-amylase (PPA), an enzyme belonging to the alpha-amylase family, is involved in the degradation of starch. Like some other members of this family, PPA requires chloride to reach maximum activity levels. To further explain the mechanism of chloride activation, a crystal of wild-type PPA soaked with ...

journal_title：Biochemistry

authors： Qian M,Ajandouz el H,Payan F,Nahoum V

更新日期：2005-03-08 00:00:00

abstract：:β-2-Microglobulin (β2m) forms amyloid fibrils in the joints of patients undergoing dialysis treatment as a result of kidney failure. One of the ways in which β2m can be induced to form amyloid fibrils in vitro is via incubation with stoichiometric amounts of Cu(II). To better understand the structural changes caused b...

journal_title：Biochemistry

authors： Borotto NB,Zhang Z,Dong J,Burant B,Vachet RW

更新日期：2017-02-28 00:00:00

abstract：:ORF56 is a small and thermodynamically extremely stable dimeric protein from the archaeon Sulfolobus islandicus. This DNA binding protein is encoded on plasmid pRN1 and possibly controls the copy number of the plasmid. We report the solution NMR structure as well as the crystal structure of ORF56 comprising a ribbon-h...

journal_title：Biochemistry

authors： Weininger U,Zeeb M,Neumann P,Löw C,Stubbs MT,Lipps G,Balbach J

更新日期：2009-10-27 00:00:00

abstract：:Resonance Raman and UV-visible absorption spectra show that nickel(II) microperoxidase-11 (NiMP-11) is four-coordinate in aqueous solution in the pH range from 1.0 to 13.0. In aqueous solutions of NiMP-11 in the absence of cetyltrimethylammonium bromide (CTAB), NiMP-11 is aggregated. In CTAB micellar solutions, where ...

journal_title：Biochemistry

authors： Ma JG,Vanderkooi JM,Zhang J,Jia SL,Shelnutt JA

更新日期：1999-03-02 00:00:00

abstract：:The recently identified Krokinobacter rhodopsin 2 (KR2) functions as a light-driven sodium ion pump. The structure of the retinal-binding pocket of KR2 offers important insights into the mechanisms of KR2, which has motif of Asn112, Asp116, and Gln123 (NDQ) that is common among sodium ion pump rhodopsins but is unique...

journal_title：Biochemistry

authors： Shigeta A,Ito S,Inoue K,Okitsu T,Wada A,Kandori H,Kawamura I

更新日期：2017-01-31 00:00:00

abstract：:Time-resolved resonance Raman spectroscopy has been employed to monitor geminate heme-CO rebinding in photolyzed HbCO. The excitation frequency was tuned to enhance the scattering from rebound heme sites 20-500 ns subsequent to CO photolysis. The behavior of vFe-C during ligand rebinding has important ramifications co...

journal_title：Biochemistry

authors： Schneebeck MC,Vigil LE,Friedman JM,Chavez MD,Ondrias MR

更新日期：1993-02-09 00:00:00

abstract：:Microdomains known as "rafts" have been isolated from many cell types as detergent-resistant membranes (DRMs) and are enriched in sphingolipids and cholesterol. However, there has been considerable controversy over whether such domains are found in native membranes or are artificially generated by the purification pro...

journal_title：Biochemistry

authors： Luria A,Vegelyte-Avery V,Stith B,Tsvetkova NM,Wolkers WF,Crowe JH,Tablin F,Nuccitelli R

更新日期：2002-11-05 00:00:00

abstract：:Using 125I-labeled phenol-alanine sparsomycin, an analogue of sparsomycin having higher biological activity than the unmodified antibiotic, we studied the requirements and the characteristics of its interaction with the ribosome. The drug does not bind to either isolated ribosomal subunits or reconstituted whole ribos...

journal_title：Biochemistry

authors： Lazaro E,van den Broek LA,San Felix A,Ottenheijm HC,Ballesta JP

更新日期：1991-10-08 00:00:00

abstract：:A novel truncated form (residues 1-214, with a randomized C-terminal tail) of the ligand-binding extracellular domain (ECD) of the human alpha1 glycine receptor (GlyR), with amino acids from the corresponding sequence of an acetylcholine binding protein (AChBP) substituted for two relatively hydrophobic membrane-proxi...

journal_title：Biochemistry

authors： Liu Z,Ramanoudjame G,Liu D,Fox RO,Jayaraman V,Kurnikova M,Cascio M

更新日期：2008-09-16 00:00:00

abstract：:The bacterium Caulobacter crescentus is known to attach irreversibly to underwater surfaces by utilizing an adhesive structure called the holdfast, which exhibits the greatest known adhesive strength of any organism. The very small size of the holdfast (∼400 nm wide and ∼40 nm high) has made direct chemical analysis d...

journal_title：Biochemistry

authors： Nyarko A,Singla S,Barton HA,Dhinojwala A

更新日期：2020-09-22 00:00:00

abstract：:The solution structure of the self-complementary dodecamer 5'd(CGCGPATTCGCG)2, containing a purine-thymine base pair within the hexameric canonical recognition site GAATTC for the restriction endonuclease EcoRI, is investigated by nuclear magnetic resonance spectroscopy and restrained molecular dynamics. Nonexchangeab...

journal_title：Biochemistry

authors： Clore GM,Oschkinat H,McLaughlin LW,Benseler F,Happ CS,Happ E,Gronenborn AM

更新日期：1988-05-31 00:00:00