Synergistic activation of protein kinase Calpha, -betaI, and -gamma isoforms induced by diacylglycerol and phorbol ester: roles of membrane association and activating conformational changes.

abstract：:The molecular organization of sterols in liposomes of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) at 37 degrees C is examined by utilizing the fluorescent analogue of cholesterol cholesta-5,7,9-trien-3 beta-ol (cholestatrienol). (1) Cholestatrienol is shown to be indistinguishable from native cholesterol i...

journal_title：Biochemistry

authors： Hyslop PA,Morel B,Sauerheber RD

更新日期：1990-01-30 00:00:00

abstract：:The cystic fibrosis transmembrane conductance regulator forms an anion-selective channel. We previously showed that charge selectivity, the ability to discriminate between anions and cations, occurs near the cytoplasmic end of the channel. The molecular determinants of charge selectivity, however, are unknown. We inve...

journal_title：Biochemistry

authors： Guinamard R,Akabas MH

更新日期：1999-04-27 00:00:00

abstract：:Z-Ala-Ala-Phe-glyoxal (where Z is benzyloxycarbonyl) has been shown to be a competitive inhibitor of pepsin with a Ki = 89 +/- 24 nM at pH 2.0 and 25 degrees C. Both the ketone carbon (R13COCHO) and the aldehyde carbon (RCO13CHO) of the glyoxal group of Z-Ala-Ala-Phe-glyoxal have been 13C-enriched. Using 13C NMR, it h...

journal_title：Biochemistry

authors： Cosgrove S,Rogers L,Hewage CM,Malthouse JP

更新日期：2007-10-02 00:00:00

abstract：:Apurinic/apyrimidinic endonuclease 1 (APE1) is an Mg²⁺-dependent enzyme responsible for incising the DNA backbone 5' to an apurinic/apyrimidinic (AP) site. Here, we use rapid quench flow (RQF) techniques to provide a comprehensive kinetic analysis of the strand-incision activity (k(chemistry)) of APE1 acting on an aut...

journal_title：Biochemistry

authors： Schermerhorn KM,Delaney S

更新日期：2013-10-29 00:00:00

abstract：:Identification of the kinetic intermediates in GTP hydrolysis on microtubules and characterization of their assembly properties is essential in understanding microtubule dynamics. By using an improved glass filter assay that selectively traps microtubules with a dead time of 2 s and monitoring taxol-induced rapid asse...

journal_title：Biochemistry

authors： Melki R,Carlier MF,Pantaloni D

更新日期：1990-09-25 00:00:00

abstract：:Cytomegaloviruses (CMVs) are ubiquitous species-specific viruses that establish acute, persistent, and latent infections. Both human and mouse CMVs encode proteins that inhibit the activation of natural killer (NK) cells by downregulating cellular ligands for the NK cell activating receptor, NKG2D. The MCMV glycoprote...

journal_title：Biochemistry

authors： Zhi L,Mans J,Paskow MJ,Brown PH,Schuck P,Jonjić S,Natarajan K,Margulies DH

更新日期：2010-03-23 00:00:00

abstract：:We have measured the base-pair lifetimes in oligodeoxynucleotides containing tracts of A.T base pairs using imino proton magnetic resonance. When the tract contains more than four consecutive A.T base pairs, possibly including a 5'-AT step but not a 5'-TA step, anomalously long lifetimes are observed. For example, the...

journal_title：Biochemistry

authors： Leroy JL,Charretier E,Kochoyan M,Guéron M

更新日期：1988-12-13 00:00:00

abstract：:CDPKs are a family of calcium (Ca2+)-dependent protein kinases which are defined by a carboxyl-terminal calmodulin-like domain. Mutational analysis indicates that the junction domain, which joins the kinase and calmodulin-like domains, contains an autoinhibitor. CDPK isoform AK1 from Arabidopsis was expressed in Esche...

journal_title：Biochemistry

authors： Harper JF,Huang JF,Lloyd SJ

更新日期：1994-06-14 00:00:00

abstract：:A kinetic folding intermediate of hen lysozyme is shown to form in a nucleation/growth type of mechanism. Under native solvent conditions, a nucleated state is formed slowly during refolding (tau = 14 +/- 1 ms at 0 M GdmCl) and is rapidly converted to the folding intermediate (tau = 300 +/- 150 micros at 0 M GdmCl). U...

journal_title：Biochemistry

authors： Kiefhaber T,Bachmann A,Wildegger G,Wagner C

更新日期：1997-04-29 00:00:00

abstract：:The interaction of the TEM-2 beta-lactamase with 9-deoxyclavulanic acid (3) and with both extensively labeled (2) and specifically labeled (1) clavulanic acid has been studied. The close similarity between 9-doexyclavulanate and clavulanate in kinetics, spectroscopic, and protein chemical terms show that the allyl alc...

journal_title：Biochemistry

authors： Charnas RL,Knowles JR

更新日期：1981-05-26 00:00:00

abstract：:It is now accepted that two or more conformations may exist within the same DNA molecule, thereby generating conformational junctions. The presence of B-Z junctions between right- and left-handed DNA conformations has been detected in plasmids by a number of techniques. Preliminary characterization of the first exampl...

journal_title：Biochemistry

authors： Sheardy RD,Winkle SA

更新日期：1989-01-24 00:00:00

abstract：:Several diseases, such as cancer, are characterized by acidification of the extracellular environment. Acidosis can be employed as a target to specifically direct therapies to the diseased tissue. We have used first principles to design an acidity-triggered rational membrane (ATRAM) peptide with high solubility in sol...

journal_title：Biochemistry

authors： Nguyen VP,Alves DS,Scott HL,Davis FL,Barrera FN

更新日期：2015-11-03 00:00:00

abstract：:Deposition of amyloid beta-peptide (Abeta) into amyloid plaques is one of the invariant neuropathological features of Alzheimer's disease. Proteins that codeposit with Abeta are potentially important for the pathogenesis, and a recently discovered plaque-associated protein is the collagenous Alzheimer amyloid plaque c...

journal_title：Biochemistry

authors： Kakuyama H,Söderberg L,Horigome K,Winblad B,Dahlqvist C,Näslund J,Tjernberg LO

更新日期：2005-11-29 00:00:00

abstract：:Photolyases (PHRs) repair the UV-induced photoproducts, cyclobutane pyrimidine dimer (CPD) or pyrimidine-pyrimidone (6-4) photoproduct [(6-4) PP], restoring normal bases to maintain genetic integrity. CPD and (6-4) PP are repaired by substrate-specific PHRs, CPD PHR and (6-4) PHR, respectively. Flavin adenine dinucleo...

journal_title：Biochemistry

authors： Yamada D,Yamamoto J,Zhang Y,Iwata T,Hitomi K,Getzoff ED,Iwai S,Kandori H

更新日期：2016-02-02 00:00:00

abstract：:The 19-transmembrane multisubunit γ-secretase complex generates the amyloid β-peptide (Aβ) of Alzheimer's disease (AD) by intramembrane proteolysis of the β-amyloid precursor protein (APP). Despite substantial advances in elucidating how this protein complex functions, the effect of the local membrane lipid microenvir...

journal_title：Biochemistry

authors： Holmes O,Paturi S,Ye W,Wolfe MS,Selkoe DJ

更新日期：2012-05-01 00:00:00

abstract：:The nucleotide binding capacity and affinity of the isolated beta subunit from Escherichia coli F1-ATPase have been studied with radiolabeled ADP and ATP by an equilibrium dialysis technique. Each mole of beta subunit in the presence of EDTA bound 1 mol of ADP or ATP with Kd values of 25 microM and 50-100 microM, resp...

journal_title：Biochemistry

authors： Issartel JP,Vignais PV

更新日期：1984-12-18 00:00:00

abstract：:Elongation factor-2 kinase (eEF-2K) is a Ca(2+)/calmodulin-dependent protein kinase that phosphorylates and inactivates eEF-2 and that can regulate the rate of protein synthesis at the elongation stage. Here we report that a slight decrease in pH, within the range observed in vivo, leads to a dramatic activation of eE...

journal_title：Biochemistry

authors： Dorovkov MV,Pavur KS,Petrov AN,Ryazanov AG

更新日期：2002-11-12 00:00:00

abstract：:In reconstituted vesicles above the lipid phase transition temperature, bacteriorhodopsin (BR) undergoes rotational diffusion about an axis perpendicular to the plane of the bilayer [Cherry, R. J., Muller, U., & Schneider, G. (1977) FEBS Lett. 80, 465]. This diffusion narrows the 13C NMR powder line shape of the BR pe...

journal_title：Biochemistry

authors： Lewis BA,Harbison GS,Herzfeld J,Griffin RG

更新日期：1985-08-13 00:00:00

abstract：:Human type beta 2 transforming growth factor (hTGF-beta 2) was purified from tamoxifen-supplemented, serum-free medium conditioned by the human prostatic adenocarcinoma cell line PC-3. The purification of hTGF-beta 2 was monitored in a growth inhibition assay and was achieved by batch purification on methylsilyl-contr...

journal_title：Biochemistry

authors： Ikeda T,Lioubin MN,Marquardt H

更新日期：1987-05-05 00:00:00

abstract：:Nitrogenase catalyzes the reduction of N2 to NH3, supporting all biological nitrogen fixation. Electron donors to this enzyme are ferredoxin or flavodoxin (in vivo) and sodium dithionite (in vitro). Features of these electron donors put a limit on spectrophotometric studies and electrocatalytic applications of nitroge...

journal_title：Biochemistry

authors： Badalyan A,Yang ZY,Hu B,Luo J,Hu M,Liu TL,Seefeldt LC

更新日期：2019-11-19 00:00:00

abstract：:The X-ray crystal structure of a salicylhydroxamic acid (SHA) inhibitory complex with human myeloperoxidase (MPO) has been determined at 2.3 A resolution. The aromatic ring of the inhibitor binds to a hydrophobic region at the entrance to the distal heme pocket between heme pyrrole ring D and the side chain of Arg 239...

journal_title：Biochemistry

authors： Davey CA,Fenna RE

更新日期：1996-08-20 00:00:00

abstract：:Nuclease footprinting techniques have been employed to investigate the interaction between the diuretic drug amiloride, a sodium channel blocker with potential therapeutic use in the treatment of cystic fibrosis, and three DNA fragments of defined sequence. Using either DNAse I or micrococcal nuclease as probes, an un...

journal_title：Biochemistry

authors： Bailly C,Cuthbert AW,Gentle D,Knowles MR,Waring MJ

更新日期：1993-03-16 00:00:00

abstract：:The E-domain of staphylococcal protein A is one of five homologous IgG-binding domains designated E, D, A, B, and C that comprise the extracellular portion of protein A. The E-domain binds tightly to Fc fragments of IgG and binds certain Fv fragments with micromolar affinity. To explore further the structural features...

journal_title：Biochemistry

authors： Starovasnik MA,Skelton NJ,O'Connell MP,Kelley RF,Reilly D,Fairbrother WJ

更新日期：1996-12-03 00:00:00

abstract：:The innate immune response to infection or injury involves an antigen-antibody triggered classical pathway (CP) of complement activation, in which soluble and cell surface plasma proteins cooperatively effect elimination of foreign organisms and damaged host cells. However, protracted or dysfunctional complement activ...

journal_title：Biochemistry

authors： Halili MA,Ruiz-Gómez G,Le GT,Abbenante G,Fairlie DP

更新日期：2009-09-08 00:00:00

abstract：:Using proton magnetic resonance, we have investigated imino and amino proton exchange in the Z form of the four oligomers d(Cbr8GCGCbr8G), d(CGm5CGCG), d(CG)6, and d(CG)12. In the latter two oligomers, all five exchangeable protons have been assigned. We find that proton acceptors such as NH3 or the basic form of Tris...

journal_title：Biochemistry

authors： Kochoyan M,Leroy JL,Guéron M

更新日期：1990-05-22 00:00:00

abstract：:Quinone oxidoreductase 2 (QR2) purified from human red blood cells was recently shown to be a potential target of the quinoline antimalarial compounds [Graves et al., (2002) Mol. Pharmacol. 62, 1364]. QR2 catalyzes the two-electron reduction of menadione via the oxidation of N-alkylated or N-ribosylated nicotinamides....

journal_title：Biochemistry

authors： Kwiek JJ,Haystead TA,Rudolph J

更新日期：2004-04-20 00:00:00

abstract：:The role of pyridoxal 5'-phosphate (PLP) in the radical-mediated amino group migration catalyzed by lysine 2,3-aminomutase from Clostridia SB4 has been investigated by electron spin echo envelope modulation (ESEEM) spectroscopy. This pulsed electron paramagnetic resonance (EPR) method was used to estimate the distance...

journal_title：Biochemistry

authors： Ballinger MD,Frey PA,Reed GH,LoBrutto R

更新日期：1995-08-08 00:00:00

abstract：:The first epidermal growth factor-like domain (EGF-1) from blood coagulation factor VII (FVII) contains two unusual O-linked glycosylation sites at Ser-52 and Ser-60. We report here a detailed study of the effect of O-fucosylation at Ser-60 on the structure of FVII EGF-1, its Ca2+-binding affinity, and its interaction...

journal_title：Biochemistry

authors： Kao YH,Lee GF,Wang Y,Starovasnik MA,Kelley RF,Spellman MW,Lerner L

更新日期：1999-06-01 00:00:00

abstract：:Each regulatory subunit of the cAMP-dependent protein kinase contains two in-tandem cAMP binding sites. Photolabeling of holoenzyme I with 8-azidoadenosine 3',5'-monophosphate (8-N3-cAMP) leads to the covalent modification of two residues, Trp-260 and Tyr-371. In order to correlate photolabeling of these two residues ...

journal_title：Biochemistry

authors： Bubis J,Taylor SS

更新日期：1987-06-16 00:00:00

abstract：:A new type of thrombin exo-site inhibitor has been designed with enhanced inhibitory potency and increased metabolic stability. With the aid of the model of the structure of the thrombin-hirudin fragment complex [Yue, S.-Y., DiMaio, J., Szewczuk, Z., Purisima, E. O., Ni, F., & Konishi, Y. (1992) Protein Eng. 5, 77-85]...

journal_title：Biochemistry

authors： Szewczuk Z,Gibbs BF,Yue SY,Purisima EO,Konishi Y

更新日期：1992-09-29 00:00:00