Direct measurement of nucleation and growth rates in lysozyme folding.

abstract：:Nitrogenase reduces N2 to NH3, but the mechanistic details are unclear. Diazene (N2H2), a proposed 2e-/2H+ intermediate on the reduction pathway, is labile under typical enzyme assay conditions, and no firm evidence is available on whether or not it can be reduced by or inhibit nitrogenase. In this paper, we compare t...

journal_title：Biochemistry

authors： McKenna CE,Simeonov AM,Eran H,Bravo-Leerabhandh M

更新日期：1996-04-09 00:00:00

abstract：:During DNA replication, the presence of oligonucleotides with partial homology to the template strand was shown to induce a switch of the polymerase from the normal template to the oligonucleotide. The latter acted as a dead-end template and led to abortive replication. The only prerequisite was that the oligonucleoti...

journal_title：Biochemistry

authors： Guieysse AL,Praseuth D,Grigoriev M,Harel-Bellan A,Hélène C

更新日期：1995-07-18 00:00:00

abstract：:Phosphoenolthiopyruvate, the analogue of phosphoenolpyruvate in which the bridging oxygen of the phosphate ester is replaced by sulfur, has been synthesized from methyl acrylate and dimethyl (chlorothio)phosphonate. The compound is a substrate for alkaline phosphatase, pyruvate kinase, enolase, and phosphoenolpyruvate...

journal_title：Biochemistry

authors： Sikkema KD,O'Leary MH

更新日期：1988-02-23 00:00:00

abstract：:DNA-DNA interstrand cross-links are thought to be important for the cytotoxicity of many chemotherapeutic agents. To study this more definitively, adduct site-specific methods are used to construct a plasmid with a single nitrogen mustard interstrand cross-link (inter-HN2-pTZSV28). Replication efficiency (RE = [coloni...

journal_title：Biochemistry

authors： Berardini M,Mackay W,Loechler EL

更新日期：1997-03-25 00:00:00

abstract：:Light-induced hypsochromic shifts of the Q(y) absorption band of the bacteriochlorophyll dimer (P) from 865 to 850 nm were identified using continuous illumination of dark-adapted reaction centers (RCs) from Rhodobacter capsulatus when dispersed in the most commonly used detergent, the zwitterionic lauryl N-dimethylam...

journal_title：Biochemistry

authors： Deshmukh SS,Akhavein H,Williams JC,Allen JP,Kalman L

更新日期：2011-06-14 00:00:00

abstract：:The electrophoretic mobilities of 24 single-stranded DNA oligomers, each containing 26 nucleotide residues, have been measured in polyacrylamide gels and in free solution. The mobilities observed at 20 degrees C differed by approximately 20% in polyacrylamide gels and by approximately 10% in free solution, even though...

journal_title：Biochemistry

authors： Stellwagen E,Abdulla A,Dong Q,Stellwagen NC

更新日期：2007-09-25 00:00:00

abstract：:The mammalian iron-binding proteins lactoferrin (Lf) and transferrin (Tf) bind iron very tightly, but reversibly. Despite homologous structures and essentially identical iron binding sites, Tf begins to release iron at pH 6.0, whereas Lf retains iron to pH approximately 3.5. This difference in iron retention gives the...

journal_title：Biochemistry

authors： Peterson NA,Arcus VL,Anderson BF,Tweedie JW,Jameson GB,Baker EN

更新日期：2002-12-03 00:00:00

abstract：:Sulfhydryl-reactive derivatives of glucosamine were synthesized as potentially transportable affinity labels of the human erythrocyte hexose carrier. N-Maleoylglycyl derivatives of either 6- or 2-amino-2-deoxy-D-glucopyranose were the most potent inhibitors of 3-O-methylglucose uptake, with concentrations of half-maxi...

journal_title：Biochemistry

authors： May JM

更新日期：1989-02-21 00:00:00

abstract：:hOgg1 and FPG are the primary DNA repair enzymes responsible for removing the major guanine (G) oxidative product, namely, 7,8-dihydro-8-oxoguanine (OG), in humans and bacteria, respectively. While natural G adopts the anti conformation and forms a Watson-Crick pair with cytosine (C), OG can also adopt the syn conform...

journal_title：Biochemistry

authors： Sowlati-Hashjin S,Wetmore SD

更新日期：2018-02-20 00:00:00

abstract：:The peptidolytic reaction of HIV-1 protease has been investigated by using four oligopeptide substrates, Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Ser-Gln-Ser-Tyr-Pro-Val-Val-NH2, and Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly-NH2, that resemble two cleavage sites found within the natu...

journal_title：Biochemistry

authors： Hyland LJ,Tomaszek TA Jr,Roberts GD,Carr SA,Magaard VW,Bryan HL,Fakhoury SA,Moore ML,Minnich MD,Culp JS

更新日期：1991-08-27 00:00:00

abstract：:The denaturation of the heat shock protein DnaK induced by guanidine hydrochloride (Gdn-HCl) was investigated by circular dichroism, fluorescence, size-exclusion HPLC, and dynamic light scattering. DnaK unfolding takes place in two discrete steps. The midpoint (Cm) of the first transition (0.5 M) was shifted to higher...

journal_title：Biochemistry

authors： Palleros DR,Shi L,Reid KL,Fink AL

更新日期：1993-04-27 00:00:00

abstract：:The conformation of the trans-anti-(1S,2R,3S,4R)-N(2)-[1-(1,2,3,4-tetrahydro-2,3,4-trihydroxybenz[a]anthracenyl)]-2'-deoxyguanosyl adduct in d(G(1)G(2)C(3)A(4)G(5)X(6)T(7)G(8)G(9)T(10)G(11)).d(C(12)A(13)C(14)C(15)A(16)C(17)C(18)T(19)G(20)C(21)C(22)), bearing codon 12 of the human N-ras protooncogene (underlined), was ...

journal_title：Biochemistry

authors： Kim HY,Wilkinson AS,Harris CM,Harris TM,Stone MP

更新日期：2003-03-04 00:00:00

abstract：:The active site structures of human Q31 granzyme A, murine granzymes (A, B, C, D, E, and F), and human granzymes (A, B, and 3) isolated from cytotoxic T lymphocytes (CTL) were studied with peptide thioester substrates, peptide chloromethyl ketone, and isocoumarin inhibitors. Human Q31, murine, and human granzyme A hyd...

journal_title：Biochemistry

authors： Odake S,Kam CM,Narasimhan L,Poe M,Blake JT,Krahenbuhl O,Tschopp J,Powers JC

更新日期：1991-02-26 00:00:00

abstract：:The expression of rusticyanin in Escherichia coli and a number of mutants for Ser86 is reported. Mutations of Ser86 to Asn, Asp, Gln, and Leu were undertaken as this is an Asn residue in other structurally characterized cupredoxins, and it has been suggested that this may be partly responsible for the high redox poten...

journal_title：Biochemistry

authors： Hall JF,Kanbi LD,Harvey I,Murphy LM,Hasnain SS

更新日期：1998-08-18 00:00:00

abstract：:The family 4 uracil-DNA glycosylase from the hyperthermophilic organism Archaeoglobus fulgidus (AFUDG) is responsible for the removal of uracil in DNA as the first step in the base excision repair (BER) pathway. AFUDG contains a large solvent-exposed peptide region containing an α helix and loop anchored on each end v...

journal_title：Biochemistry

authors： Engstrom LM,Partington OA,David SS

更新日期：2012-06-26 00:00:00

abstract：:Monoamine oxidase B (MAO B) is an integral protein of the outer mitochondrial membrane that is involved in the deamination of vasoactive and neuroactive amines. The oxidation of these amine substrates requires the cofactor FAD, which is covalently bound to Cys-397 of human MAO B. Previously, Glu-34 and Tyr-44 of MAO B...

journal_title：Biochemistry

authors： Kirksey TJ,Kwan SW,Abell CW

更新日期：1998-09-01 00:00:00

abstract：:The beta(2) subunit of a class Ia or Ib ribonucleotide reductase (RNR) is activated when its carboxylate-bridged Fe(2)(II/II) cluster reacts with O(2) to oxidize a nearby tyrosine (Y) residue to a stable radical (Y(*)). During turnover, the Y(*) in beta(2) is thought to reversibly oxidize a cysteine (C) in the alpha(2...

journal_title：Biochemistry

authors： Jiang W,Yun D,Saleh L,Bollinger JM Jr,Krebs C

更新日期：2008-12-30 00:00:00

abstract：:The dissociation of Semliki Forest virus induced by increasing concentrations of the anionic detergent sodium dodecyl sulfate was studied using density gradient centrifugation. Detectable binding to the virus started well below the critical micellar concentration of the detergent and increased thereafter with increase...

journal_title：Biochemistry

authors： Becker R,Helenius A,Simons K

更新日期：1975-05-06 00:00:00

abstract：:Binding of substrate to ornithine 4,5-aminomutase (OAM) and methylmalonyl-CoA mutase (MCM) leads to the formation of an electrostatic interaction between a conserved glutamate side chain and the adenosyl ribose of the adenosylcobalamin (AdoCbl) cofactor. The contribution of this residue (Glu338 in OAM from Clostridium...

journal_title：Biochemistry

authors： Makins C,Pickering AV,Mariani C,Wolthers KR

更新日期：2013-02-05 00:00:00

abstract：:NADP(H) binding is essential for fast electron transfer through the flavoprotein domain of the fusion protein P450BM3. Here we characterize the interaction of NADP(H) with the oxidized and partially reduced enzyme and the effect of this interaction on the redox properties of flavin cofactors and electron transfer. Mea...

journal_title：Biochemistry

authors： Murataliev MB,Feyereisen R

更新日期：2000-10-17 00:00:00

abstract：:Proelastase has been purified to homogeneity from rat pancreatic tissue by a combination of CM-Sephadex and immobilized protease inhibitor affinity resins. Trypsin activation yields an elastolytic enzyme that possesses a specificity toward small hydrophobic residues in synthetic amide substrates, similar to those of p...

journal_title：Biochemistry

authors： Largman C

更新日期：1983-08-02 00:00:00

abstract：:Native ribonucleotide reductase from Escherichia coli exhibits a resonance-enhanced Raman mode at 1498 cm-1 that is characteristic of a tyrosyl radical. The Raman frequency as well as the absorption maximum at 410 nm identifies the radical as being in a deprotonated state. The B2 subunit of ribonucleotide reductase sh...

journal_title：Biochemistry

authors： Backes G,Sahlin M,Sjöberg BM,Loehr TM,Sanders-Loehr J

更新日期：1989-02-21 00:00:00

abstract：:Rhodopsin kinase phosphorylates serine- and threonine-containing peptides from bovine rhodopsin's carboxyl-terminal sequence. Km's for the peptides decrease as the length of the peptide is increased over the range 12-31 amino acids, reaching 1.7 mM for peptide 318-348 from the rhodopsin sequence. The Km for phosphoryl...

journal_title：Biochemistry

authors： Palczewski K,Arendt A,McDowell JH,Hargrave PA

更新日期：1989-10-31 00:00:00

abstract：:SH2 domains are protein modules which interact with specific tyrosine phosphorylated sequences in target proteins. The SH2 domain of the Src kinase binds with high affinity to a tyrosine phosphorylated peptide containing the amino acids Glu, Glu, and Ile (EEI) at the positions +1, +2, and +3 C-terminal to the phosphot...

journal_title：Biochemistry

authors： Bradshaw JM,Waksman G

更新日期：1999-04-20 00:00:00

abstract：:Protein engineering to alter recognition underlying ligand binding and activity has enormous potential. Here, ligand binding for Escherichia coli phosphoenolpyruvate carboxykinase (PEPCK), which converts oxaloacetate into CO2 and phosphoenolpyruvate as the first committed step in gluconeogenesis, was engineered to acc...

journal_title：Biochemistry

authors： Tang HYH,Shin DS,Hura GL,Yang Y,Hu X,Lightstone FC,McGee MD,Padgett HS,Yannone SM,Tainer JA

更新日期：2018-12-04 00:00:00

abstract：:Escherichia coli DNA photolyase contains a stable flavin radical that is readily photoreduced in the presence of added electron donors. Picosecond, nanosecond, and conventional flash photolysis technique have been employed to investigate the events leading to photoreduction from 40 ps to tens of milliseconds following...

journal_title：Biochemistry

authors： Heelis PF,Okamura T,Sancar A

更新日期：1990-06-19 00:00:00

abstract：:Myc and Max belong to the b-HLH-LZ family of transcription factors. Heterodimerization between Myc and Max or homodimerization of Max allows these proteins to bind their cognate DNA sequence known as the E-box (CACGTG). Recent evidence has suggested that the c-Myc/Max heterodimeric b-HLH-LZ could interact to form a he...

journal_title：Biochemistry

authors： Lebel R,McDuff FO,Lavigne P,Grandbois M

更新日期：2007-09-11 00:00:00

abstract：:Detailed information on room-temperature structure and oxidation state of the Photosystem II (PS II) manganese complex is needed to put mechanistic considerations on solid grounds. Because previously this information had not been available, the tetranuclear manganese complex was investigated by X-ray absorption spectr...

journal_title：Biochemistry

authors： Meinke C,Solé VA,Pospisil P,Dau H

更新日期：2000-06-20 00:00:00

abstract：:The two distinct domains of flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase, EC 1.1.2.3) are connected by a typical hinge peptide. To probe the importance of the structural integrity of the hinge region for efficient intraprotein electron transfer, three mutant enzymes have been constructed: H delta 3 [Sharp...

journal_title：Biochemistry

authors： Sharp RE,Chapman SK,Reid GA

更新日期：1996-01-23 00:00:00

abstract：:The borrelidin polyketide synthase (PKS) begins with a carboxylated substrate and, unlike typical decarboxylative loading PKSs, retains the carboxy group in the final product. The specificity and tolerance of incorporation of carboxyacyl substrate into type I PKSs have not been explored. Here, we show that the first e...

journal_title：Biochemistry

authors： Hagen A,Poust S,de Rond T,Yuzawa S,Katz L,Adams PD,Petzold CJ,Keasling JD

更新日期：2014-09-30 00:00:00