当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Identification and structural characterization of an unusual RING-like sequence within an extracellular biomineralization protein, AP7.

Identification and structural characterization of an unusual RING-like sequence within an extracellular biomineralization protein, AP7.

Abstract：

:The RING or Really Interesting New Gene represents a family of eukaryotic sequences that bind Zn (II) ions and participate in intracellular processes involving protein-protein interaction. Although found in over 400 different proteins, very little is known regarding the structure-function properties of these domains because of the aggregation problems associated with RING sequences. To augment this data set, we report an unusual 36 AA C-terminal sequence of an extracellular matrix mollusk shell protein, AP7, that exhibits partial homology to the RING family. This Cys, His-containing sequence, termed AP7C, binds Zn (II) and other multivalent ions, but does not utilize a tetracoordinate complexation scheme for binding such as that found in Zn (II) finger polypeptides. Moreover, unlike Zn (II) finger and RING domains, this 36 AA can fold into a relatively stable structure in the absence of Zn (II). This folded structure consists of three short helical segments (A, B, and C), with segments A and B separated by a 4 AA type I beta-turn region and segments B and C separated by a 7 AA loop-like region. Interestingly, the putative RING-like region, -RRPFHECALCYSI-, experiences slow conformational exchange between two structural states in solution, most likely in response to imido ring interconversion at P8 and P21. Poisson-Boltzmann solvation calculations reveal that the AP7C molecular surface possesses a cationic region near its N-terminus, which lies adjacent to the 30 AA mineral modification domain in the AP7 protein. Given that the AP7C sequence does not influence mineralization, it is probable that this cationic pseudo-RING region is utilized by the AP7 protein for other tasks such as protein-protein interaction within the mollusk shell matrix.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Collino S,Kim IW,Evans JS

doi

10.1021/bi701949p

subject

Has Abstract

pub_date

2008-03-25 00:00:00

pages

3745-55

issue

12

eissn

0006-2960

issn

1520-4995

journal_volume

47

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • pH-responsive DNA-binding activity of Helicobacter pylori NikR.

    abstract：:Helicobacter pylori NikR (HpNikR) is a nickel-responsive transcription factor. In addition to a role in nickel homeostasis, HpNikR is proposed to serve as a master activator-repressor for H. pylori acid adaptation by directly or indirectly regulating the expression of a battery of genes. One potential mechanism of thi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801742r

    authors： Li Y,Zamble DB

    更新日期：2009-03-24 00:00:00

  • Ability of tetrahydrobiopterin analogues to support catalysis by inducible nitric oxide synthase: formation of a pterin radical is required for enzyme activity.

    abstract：:Pterin-free inducible nitric oxide synthase (iNOS) was reconstituted with tetrahydrobiopterin (H(4)B) or tetrahydrobiopterin analogues (5-methyl-H(4)B and 4-amino-H(4)B), and the ability of bound 5-methyl-H(4)B and 4-amino-H(4)B to support catalysis by either full-length iNOS (FLiNOS) or the isolated heme domain (HDiN...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035491p

    authors： Hurshman AR,Krebs C,Edmondson DE,Marletta MA

    更新日期：2003-11-18 00:00:00

  • Thermodynamic characterization of the osmolyte- and ligand-folded states of Bacillus subtilis ribonuclease P protein.

    abstract：:In Bacillus subtilis, P protein is the noncatalytic component of ribonuclease P (RNase P) that is critical for achieving maximal nuclease activity under physiological conditions. P protein is predominantly unfolded (D) at neutral pH and low ionic strength; however, it folds upon the addition of sulfate anions (ligands...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0504613

    authors： Henkels CH,Oas TG

    更新日期：2005-10-04 00:00:00

  • Structure and function of PA4872 from Pseudomonas aeruginosa, a novel class of oxaloacetate decarboxylase from the PEP mutase/isocitrate lyase superfamily.

    abstract：:Pseudomonas aeruginosa PA4872 was identified by sequence analysis as a structurally and functionally novel member of the PEP mutase/isocitrate lyase superfamily and therefore targeted for investigation. Substrate screens ruled out overlap with known catalytic functions of superfamily members. The crystal structure of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701954p

    authors： Narayanan BC,Niu W,Han Y,Zou J,Mariano PS,Dunaway-Mariano D,Herzberg O

    更新日期：2008-01-08 00:00:00

  • Altered APP processing in PDAPP (Val717 --> Phe) transgenic mice yields extended-length Abeta peptides.

    abstract：:Central to the pathology of Alzheimer's disease (AD) is the profuse accumulation of amyloid-beta (Abeta) peptides in the brain of affected individuals, and several amyloid precursor protein (APP) transgenic (Tg) mice models have been created to mimic Abeta deposition. Among these, the PDAPP Tg mice carrying the famili...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051213+

    authors： Esh C,Patton L,Kalback W,Kokjohn TA,Lopez J,Brune D,Newell AJ,Beach T,Schenk D,Games D,Paul S,Bales K,Ghetti B,Castaño EM,Roher AE

    更新日期：2005-10-25 00:00:00

  • Certain metal ions are inhibitors of cytochrome b6f complex 'Rieske' iron-sulfur protein domain movements.

    abstract：:Many current models of the Q cycle for the cytochrome (cyt) b6f and the cyt bc1 complexes incorporate 'Rieske' iron-sulfur protein (ISP) domain movements to gate electron transfer and to ensure high yields of proton shuttling. It was previously proposed that copper ions, which bind at a site distant from the quinol ox...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015996k

    authors： Roberts AG,Bowman MK,Kramer DM

    更新日期：2002-03-26 00:00:00

  • Mass spectrometric characterization of the human androgen receptor ligand-binding domain expressed in Escherichia coli.

    abstract：:The ligand-binding domain (LBD) of the human androgen receptor (hAR LBD), encompassing amino acids (AAs) 647-919, was expressed in Escherichia coli with an N-terminal polyhistidine tag (His(10)-hAR LBD) from a pET-16b vector. The overexpressed protein was initially insoluble in inclusion bodies, and was subsequently s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010525j

    authors： Zhu Z,Becklin RR,Desiderio DM,Dalton JT

    更新日期：2001-09-11 00:00:00

  • Mechanistic studies examining the efficiency and fidelity of DNA synthesis by the 3TC-resistant mutant (184V) of HIV-1 reverse transcriptase.

    abstract：:A single amino acid substitution from methionine-184 to valine (M184V) of HIV-1 reverse transcriptase (RT) evokes the 1000-fold 3TC (Lamivudine) resistance by the HIV-1 virus observed in the clinic. The M184V mutant HIV-1 RT was studied to assess its catalytic efficiency during single nucleotide incorporation using a ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990709m

    authors： Feng JY,Anderson KS

    更新日期：1999-07-20 00:00:00

  • The vinblastine binding site adopts high- and low-affinity conformations during a transport cycle of P-glycoprotein.

    abstract：:Conceptually one may envisage that substrate binding sites on the ABC transporter P-gp cycle between high- and low-affinity conformations in response to signals arising from nucleotide hydrolysis to effect active transport. A radioligand binding assay was used to characterize the interaction of [3H]vinblastine with P-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011211z

    authors： Martin C,Higgins CF,Callaghan R

    更新日期：2001-12-25 00:00:00

  • Identification of a region of the ileal-type sodium/bile acid cotransporter interacting with a competitive bile acid transport inhibitor.

    abstract：:Drug intervention that prevents reabsorption of circulating bile acids by the apical (ileal) sodium/bile acid cotransporter (ASBT) may be a promising new therapy for lowering of plasma cholesterol. 2164U90 is a benzothiazepine-based competitive inhibitor of bile acid transport with K(i) values of approximately 10 and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0205404

    authors： Hallén S,Björquist A,Ostlund-Lindqvist AM,Sachs G

    更新日期：2002-12-17 00:00:00

  • Proton transfer in carbonic anhydrase is controlled by electrostatics rather than the orientation of the acceptor.

    abstract：:Combined quantum mechanical/molecular mechanical (QM/MM) simulations are carried out to analyze factors that dictate the proton transfer in carbonic anhydrase II (CAII), an enzyme that has been used as a prototypical example of long-range proton transfers in biomolecules. In contrast to the long-held conjecture in the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701950j

    authors： Riccardi D,König P,Guo H,Cui Q

    更新日期：2008-02-26 00:00:00

  • Mechanistic studies unravel the complexity inherent in tau aggregation leading to Alzheimer's disease and the tauopathies.

    abstract：:The aggregation of the protein tau into amyloid fibrils is known to be involved in the causation of the neurodegenerative tauopathies and the progression of cognitive decline in Alzheimer's disease. This review surveys the mechanism of tau aggregation with special emphasis on the information obtained from biochemical ...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi400209z

    authors： Ramachandran G,Udgaonkar JB

    更新日期：2013-06-18 00:00:00

  • 2-Hydroxy-2'-deoxyadenosine 5'-triphosphate enhances A.T --> C.G mutations caused by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate by suppressing its degradation upon replication in a HeLa extract.

    abstract：:The coexistence effects of multiple kinds of oxidized deoxyribonucleotides were examined using an SV40 origin-dependent in vitro replication system with a HeLa extract. Oxidized dGTP and dATP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP) and 2-hydroxy-2'-deoxyadenosine 5'-triphosphate (2-OH-dATP), were used...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi062117r

    authors： Satou K,Kasai H,Masutani C,Hanaoka F,Harashima H,Kamiya H

    更新日期：2007-06-05 00:00:00

  • The ribotoxin restrictocin recognizes its RNA substrate by selective engagement of active site residues.

    abstract：:Restrictocin and related fungal endoribonucleases from the α-sarcin family site-specifically cleave the sarcin/ricin loop (SRL) on the ribosome to inhibit translation and ultimately trigger cell death. Previous studies showed that the SRL folds into a bulged-G motif and tetraloop, with restrictocin achieving a specifi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1018336

    authors： Plantinga MJ,Korennykh AV,Piccirilli JA,Correll CC

    更新日期：2011-04-12 00:00:00

  • Characterization of the macrolide P-450 hydroxylase from Streptomyces venezuelae which converts narbomycin to picromycin.

    abstract：:The post-polyketide synthase (PKS) biosynthetic tailoring of macrolide antibiotics usually involves one or more oxidation reactions catalyzed by cytochrome P450 monooxygenases. As the specificities of members from this class of enzymes vary significantly among PKS gene clusters, the identification and study of new mac...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981699c

    authors： Betlach MC,Kealey JT,Ashley GW,McDaniel R

    更新日期：1998-10-20 00:00:00

  • Mass spectrometric based mapping of the disulfide bonding patterns of integrin alpha chains.

    abstract：:Integrins are one of the major mediators of cellular adherence. Structurally the component alpha and beta chains are characterized by extensive intrachain disulfide bonding. The assignment of these bonds is currently based on homology with the chains of the integrin alphaIIbbeta3. However, recent crystallographic anal...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034726u

    authors： Krokhin OV,Cheng K,Sousa SL,Ens W,Standing KG,Wilkins JA

    更新日期：2003-11-11 00:00:00

  • Structure of bovine adrenal dopamine beta-monooxygenase, as deduced from cDNA and protein sequencing: evidence that the membrane-bound form of the enzyme is anchored by an uncleaved signal peptide.

    abstract：:A full-length cDNA for dopamine beta-monooxygenase (D beta M) from bovine adrenal glands has been cloned and sequenced. The soluble and membrane-derived forms of D beta M have also been sequenced from their N-termini. While the observed sequences for the soluble protein correspond to those previously reported [Joh, T....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00452a026

    authors： Taljanidisz J,Stewart L,Smith AJ,Klinman JP

    更新日期：1989-12-26 00:00:00

  • Structural Basis of Substrate Recognition by Aldehyde Dehydrogenase 7A1.

    abstract：:Aldehyde dehydrogenase 7A1 (ALDH7A1) is part of lysine catabolism and catalyzes the NAD(+)-dependent oxidation of α-aminoadipate semialdehyde to α-aminoadipate. Herein, we describe a structural study of human ALDH7A1 focused on substrate recognition. Five crystal structures and small-angle X-ray scattering data are re...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00754

    authors： Luo M,Tanner JJ

    更新日期：2015-09-08 00:00:00

  • Digestion of RNA of chromatin and nuclear ribonucleoprotein by staphylococcal nuclease.

    abstract：:Following a 1-h [3H]uridine pulse of cells of a human colon carcinoma line, 15% of the radioactivity in heterogeneous nuclear RNA associated with both chromatin and nuclear ribonucleoprotein was not digested to acid soluble fragments during a 2-h incubation with staphylococcal nuclease (EC 3.1.4.7). These [3H]uridine-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00662a026

    authors： Augenlicht LH,McCormick M,Lipkin M

    更新日期：1976-08-24 00:00:00

  • Site-specific epitope tagging of G protein-coupled receptors by bioorthogonal modification of a genetically encoded unnatural amino acid.

    abstract：:We developed a general strategy for labeling expressed membrane proteins with a peptide epitope tag and detecting the tagged proteins in native cellular membranes. First, we genetically encoded the unnatural amino acid p-azido-L-phenylalanine (azF) at various specific sites in a G protein-coupled receptor (GPCR), C-C ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi301292h

    authors： Naganathan S,Ye S,Sakmar TP,Huber T

    更新日期：2013-02-12 00:00:00

  • Unique guanine nucleotide binding properties of the human placental GTP-binding protein Gp.

    abstract：:Gp is a major GTP-binding protein of human placenta and platelets [Evans, T., Brown, M. L., Fraser, E. D., & Northup, J. K. (1986) J. Biol. Chem. 261, 7052-7059]. High-affinity guanine nucleotide binding is associated with a polypeptide migrating identically with H-ras on SDS-PAGE. We have characterized the interactio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00482a002

    authors： Tamir A,Fawzi AB,Northup JK

    更新日期：1990-07-31 00:00:00

  • Disulfide bond assignment in human J chain and its covalent pairing with immunoglobulin M.

    abstract：:The assignment of disulfide bonds in human J chain and its covalent pairing with immunoglobulin M was determined under conditions which minimize disulfide bond interchange. We show that in J chain the three intradisulfide bridges are formed between Cys 12 and 100, Cys 71 and 91, and Cys 108 and 133. Previous reports [...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00165a014

    authors： Frutiger S,Hughes GJ,Paquet N,Lüthy R,Jaton JC

    更新日期：1992-12-22 00:00:00

  • Following the rotational trajectory of the principal hydrodynamic frame of a protein using multiple probes.

    abstract：:A generalized set of fluorescence polarization intensities and electron paramagnetic resonance (EPR) spectra from multiple fluorescent and EPR probes of a specific site on an oriented and immobilized protein element in a biological assembly, combined with anisotropic rotational relaxation studies of the purified and l...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00184a004

    authors： Burghardt TP,Ajtai K

    更新日期：1994-05-10 00:00:00

  • DNA translocation by human uracil DNA glycosylase: the case of single-stranded DNA and clustered uracils.

    abstract：:Human uracil DNA glycosylase (hUNG) plays a central role in DNA repair and programmed mutagenesis of Ig genes, requiring it to act on sparsely or densely spaced uracil bases located in a variety of contexts, including U/A and U/G base pairs, and potentially uracils within single-stranded DNA (ssDNA). An interesting qu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi301562n

    authors： Schonhoft JD,Stivers JT

    更新日期：2013-04-16 00:00:00

  • Enzyme-induced strain/distortion in the ground-state ES complex in beta-lactamase catalysis revealed by FTIR.

    abstract：:Class A beta-lactamases hydrolyze penicillins and other beta-lactams via an acyl-enzyme catalytic mechanism. Ser70 is the active site nucleophile. By constructing the S70A mutant, which is unable to form the acyl-enzyme intermediate, it was possible to make stable ES complexes with various substrates. The stability of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9928041

    authors： Hokenson MJ,Cope GA,Lewis ER,Oberg KA,Fink AL

    更新日期：2000-05-30 00:00:00

  • A new chemical mechanism catalyzed by a mutated aldehyde dehydrogenase.

    abstract：:NAD(P) aldehyde dehydrogenases (EC 1.2.1.3) are a family of enzymes that oxidize a wide variety of aldehydes into acid or activated acid compounds. Using site-directed mutagenesis, the essential nucleophilic Cys 149 in the NAD-dependent phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Escherichia coli has...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00164a033

    authors： Corbier C,Della Seta F,Branlant G

    更新日期：1992-12-15 00:00:00

  • Narrowing substrate specificity in a directly evolved enzyme: the A293D mutant of aspartate aminotransferase.

    abstract：:Several mutant Escherichia coli aspartate aminotransferases (eAATases) have been characterized in the attempt to evolve or rationally redesign the substrate specificity of eAATase into that of E. coli tyrosine aminotransferase (eTATase). These include HEX (designed), HEX + A293D (design followed by directed evolution)...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0487544

    authors： Chow MA,McElroy KE,Corbett KD,Berger JM,Kirsch JF

    更新日期：2004-10-12 00:00:00

  • Slow-binding inhibition of Escherichia coli cystathionine beta-lyase by L-aminoethoxyvinylglycine: a kinetic and X-ray study.

    abstract：:The pyridoxal 5'-phosphate (PLP)-dependent cystathionine beta-lyase (CBL) was previously found to be inhibited by the natural toxins rhizobitoxine and l-aminoethoxyvinylglycine (AVG). The present study characterizes the interaction of Escherichia coli CBL with AVG and methoxyvinylglycine (MVG) by a combination of kine...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970630m

    authors： Clausen T,Huber R,Messerschmidt A,Pohlenz HD,Laber B

    更新日期：1997-10-14 00:00:00

  • Stereoselective Formation of Multiple Reaction Products by the Phosphotriesterase from Sphingobium sp. TCM1.

    abstract：:Organophosphate flame retardants are used to inhibit combustion and increase plasticity in plastics and durable foams. While not neurotoxic, these compounds are potential carcinogens, endocrine disrupters, and developmental toxins. The phosphotriesterase from Sphingobium sp. TCM1 (Sb-PTE) is unique among phosphotriest...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00089

    authors： Bigley AN,Narindoshvili T,Xiang DF,Raushel FM

    更新日期：2020-03-31 00:00:00

  • Kinetics of calcium uptake by isolated sarcoplasmic reticulum vesicles using flash photolysis of caged adenosine 5'-triphosphate.

    abstract：:The kinetics of ATP-induced Ca2+ uptake by vesicular dispersions of sarcoplasmic reticulum were determined with a time resolution of about 10 ms, depending on the temperature. Ca2+ uptake was initiated by the addition of ATP through the flash photolysis of P3-1-(2-nitrophenyl)-ethyl adenosine 5'-triphosphate utilizing...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00292a003

    authors： Pierce DH,Scarpa A,Topp MR,Blasie JK

    更新日期：1983-11-08 00:00:00