当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Identification and structural characterization of an unusual RING-like sequence within an extracellular biomineralization protein, AP7.

Identification and structural characterization of an unusual RING-like sequence within an extracellular biomineralization protein, AP7.

Abstract：

:The RING or Really Interesting New Gene represents a family of eukaryotic sequences that bind Zn (II) ions and participate in intracellular processes involving protein-protein interaction. Although found in over 400 different proteins, very little is known regarding the structure-function properties of these domains because of the aggregation problems associated with RING sequences. To augment this data set, we report an unusual 36 AA C-terminal sequence of an extracellular matrix mollusk shell protein, AP7, that exhibits partial homology to the RING family. This Cys, His-containing sequence, termed AP7C, binds Zn (II) and other multivalent ions, but does not utilize a tetracoordinate complexation scheme for binding such as that found in Zn (II) finger polypeptides. Moreover, unlike Zn (II) finger and RING domains, this 36 AA can fold into a relatively stable structure in the absence of Zn (II). This folded structure consists of three short helical segments (A, B, and C), with segments A and B separated by a 4 AA type I beta-turn region and segments B and C separated by a 7 AA loop-like region. Interestingly, the putative RING-like region, -RRPFHECALCYSI-, experiences slow conformational exchange between two structural states in solution, most likely in response to imido ring interconversion at P8 and P21. Poisson-Boltzmann solvation calculations reveal that the AP7C molecular surface possesses a cationic region near its N-terminus, which lies adjacent to the 30 AA mineral modification domain in the AP7 protein. Given that the AP7C sequence does not influence mineralization, it is probable that this cationic pseudo-RING region is utilized by the AP7 protein for other tasks such as protein-protein interaction within the mollusk shell matrix.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Collino S,Kim IW,Evans JS

doi

10.1021/bi701949p

subject

Has Abstract

pub_date

2008-03-25 00:00:00

pages

3745-55

issue

12

eissn

0006-2960

issn

1520-4995

journal_volume

47

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • (Na, K)ATPase activity in membrane preparations of ouabain-resistant HeLa cells.

    abstract：:Membrane preparations from two independent ouabain-resistant HeLa cell clones, HI-B1 and HI-C1, each appear to contain two species of (Na,K)ATPase. Two-thirds of the total (Na,K)ATPase in each mutant is indistinguishable from the enzyme in preparations of wild type cells with respect to ouabain binding, ouabain inhibi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00642a600

    authors： Robbins AR,Baker RM

    更新日期：1977-11-15 00:00:00

  • The complete amino acid sequence of a cardiotoxin from the venom of Naja naja (Cambodian Cobra).

    abstract：:The complete amino acid sequence of a small, basic protein with cardiotoxic activity is described. This toxin, designated Naja naja F8, was isolated from the venom of Naja naja, of Cambodian origin, by gel filtration on Sephadex G-75 followed by gradient ion exchange chromatography on Bio-Rex 70. The cardiotoxin F8, m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00684a011

    authors： Fryklund L,Eaker D

    更新日期：1975-07-01 00:00:00

  • Two promoters in the bovine adrenodoxin gene and the role of associated, unique cAMP-responsive sequences.

    abstract：:The bovine adrenodoxin gene gives rise to two species of mRNA differing only at their 5'-ends. The synthesis of these two types of mRNA in bovine adrenal cortical cells is regulated transcriptionally in part by ACTH via the action of cAMP. Examination of the 5'-end of the adrenodoxin gene revealed that each mRNA conta...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00123a027

    authors： Chen JY,Waterman MR

    更新日期：1992-03-03 00:00:00

  • Chemical Interactions of Polyethylene Glycols (PEGs) and Glycerol with Protein Functional Groups: Applications to Effects of PEG and Glycerol on Protein Processes.

    abstract：:In this work, we obtain the data needed to predict chemical interactions of polyethylene glycols (PEGs) and glycerol with proteins and related organic compounds and thereby interpret or predict chemical effects of PEGs on protein processes. To accomplish this, we determine interactions of glycerol and tetraEG with >30...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00246

    authors： Knowles DB,Shkel IA,Phan NM,Sternke M,Lingeman E,Cheng X,Cheng L,O'Connor K,Record MT

    更新日期：2015-06-09 00:00:00

  • Proton nuclear magnetic resonance investigation of cross-linked asymmetrically modified hemoglobins: influence of the salt bridges on tertiary and quaternary structures of hemoglobin.

    abstract：:Asymmetrically modified hemoglobins, [alpha(des-Arg)beta]A[alpha beta]cXL, [alpha(des-Arg-Tyr)beta]A[alpha beta]cXL, [alpha(des-Arg)beta(NES)]A[alpha beta]cXL, and [alpha(des-Arg)beta]A[alpha beta(NES)]cXL, have been prepared from chemically modified human normal adult hemoglobin (Hb A) and mutant hemoglobin C (beta 6...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00306a027

    authors： Miura S,Ho C

    更新日期：1984-05-22 00:00:00

  • Identification by MALDI-TOF mass spectrometry of 17 alpha-bromoacetamidopropylestradiol covalent attachment sites on estrogen receptor alpha.

    abstract：:Mass spectrometry was used to identify the sites of covalent attachment of [(14)C]-17alpha-bromoacetamidopropylestradiol ([(14)C]17BAPE(2), an estradiol agonist) to the ligand-binding domain (LBD) of mouse estrogen receptor alpha (ERalpha). A glutathione S-transferase (GST)-LBD chimera protein was overexpressed in Esc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0205092

    authors： Mattras H,Aliau S,Richard E,Bonnafous JC,Jouin P,Borgna JL

    更新日期：2002-12-31 00:00:00

  • Alternatively spliced form of human thyroperoxidase, TPOzanelli: activity, intracellular trafficking, and role in hormonogenesis.

    abstract：:Thyroperoxidase (TPO), a type I transmembrane heme containing glycoprotein, catalyzes iodide organification and thyroid hormone synthesis. One of the two main alternatively spliced forms of this enzyme, TPOzanelli, which is present in Graves's disease thyroid tissue, has a cytoplasmic domain completely modified. In th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0026324

    authors： Niccoli-Sire P,Fayadat L,Siffroi-Fernandez S,Malthierry Y,Franc JL

    更新日期：2001-02-27 00:00:00

  • Essential sulfhydryl for reduced nicotinamide adenine dinucleotide binding in D-beta-hydroxybutyrate dehydrogenase.

    abstract：:Chemical derivatization studies have been directed at the sulfhydryl group of D-beta-hydroxybutyrate dehydrogenase, a lipid-requiring enzyme. Reaction with N-ethylmaleimide leads to progressive and parallel loss of both enzymic activity and coenzyme binding. Both functions are lost when 1 equiv of sulfhydryl is deriva...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00564a021

    authors： Latruffe N,Brenner SC,Fleischer S

    更新日期：1980-11-11 00:00:00

  • Amino acid sequence of ragweed allergen Ra3.

    abstract：:The complete amino acid sequence of ragweed pollen allergen Ra3 has been determined. The molecule consists of 101 amino acid residues and to date is the only allergen isolated from Ambrosia elatior (short ragweed) which contains carbohydrate. This particular preparation of allergen has a single, unique amino acid sequ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00566a010

    authors： Klapper DG,Goodfriend L,Capra JD

    更新日期：1980-12-09 00:00:00

  • Transient formation of a neutral ubisemiquinone radical and subsequent intramolecular electron transfer to pyrroloquinoline quinone in the Escherichia coli membrane-integrated glucose dehydrogenase.

    abstract：:The membrane-bound quinoprotein glucose dehydrogenase (mGDH) in Escherichia coli contains pyrroloquinoline quinone (PQQ) and participates in the direct oxidation of D-glucose to D-gluconate by transferring electrons to ubiquinone (UQ). To elucidate the mechanism of ubiquinone reduction by mGDH, we applied a pulse radi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051347n

    authors： Kobayashi K,Mustafa G,Tagawa S,Yamada M

    更新日期：2005-10-18 00:00:00

  • Indirect readout of tRNA for aminoacylation.

    abstract：:Aminoacylation of tRNA by aminoacyl-tRNA synthetases is the essential reaction that matches protein amino acids with the trinucleotide sequences specified in mRNA. Direct electrostatic interactions made by tRNA synthetases with discriminating functional groups on the tRNA bases have long been known to determine aminoa...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi7014647

    authors： Perona JJ,Hou YM

    更新日期：2007-09-18 00:00:00

  • Effects of DNA binding proteins on DNA methylation in vitro.

    abstract：:The inheritance of DNA methylation patterns may play an important role in the stability of the differentiated state. We have therefore studied the inhibitory effects of DNA binding proteins on DNA methylation in vitro. Mouse L1210 cells grown in the presence of 5-azacytidine acquire hemimethylated sites in their DNA. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00326a021

    authors： Kautiainen TL,Jones PA

    更新日期：1985-02-26 00:00:00

  • Trehalose-containing lipooligosaccharides of Mycobacterium gordonae: presence of a mono-O-methyltetra-O-acyltrehalose "core" and branching in the oligosaccharide backbone.

    abstract：:Past evidence has indicated that Mycobacterium gordonae, as isolated from soil and as an occasional opportunistic pathogen, exists as a serocomplex. We now demonstrate that the basis of seroreactivity and diversity is a novel series of alkali-labile, trehalose-containing lipooligosaccharides (LOS). The structures from...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00210a020

    authors： Besra GS,McNeil MR,Khoo KH,Dell A,Morris HR,Brennan PJ

    更新日期：1993-11-30 00:00:00

  • Activation-induced subcellular redistribution of G alpha(s) is dependent upon its unique N-terminus.

    abstract：:The heterotrimeric G protein subunit, alpha(s), can move reversibly from plasma membranes to cytoplasm in response to activation by GPCRs or activating mutations. We examined the importance of the unique N-terminus of alpha(s) in this translocation in cultured cells. alpha(s) contains a single site for palmitoylation ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi025533u

    authors： Thiyagarajan MM,Bigras E,Van Tol HH,Hébert TE,Evanko DS,Wedegaertner PB

    更新日期：2002-07-30 00:00:00

  • Structure and catalytic mechanism of N(5),N(10)-methenyl-tetrahydromethanopterin cyclohydrolase.

    abstract：:Methenyltetrahydromethanopterin (methenyl-H(4)MPT(+)) cyclohydrolase (Mch) catalyzes the interconversion of methenyl-H(4)MPT(+) and formyl-H(4)MPT in the one-carbon energy metabolism of methanogenic, methanotrophic, and sulfate-reducing archaea and of methylotrophic bacteria. To understand the catalytic mechanism of t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300777k

    authors： Upadhyay V,Demmer U,Warkentin E,Moll J,Shima S,Ermler U

    更新日期：2012-10-23 00:00:00

  • Conservation of promoter melting mechanisms in divergent regions of the single-subunit RNA polymerases.

    abstract：:The single-subunit RNA polymerases make up a widespread family of proteins found in phage, mitochondria, and chloroplasts. Unlike the phage RNAPs, the eukaryotic RNAPs require accessory factors to melt their promoters and diverge from the phage RNAPs in the regions where functions associated with promoter melting in t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300074j

    authors： Velazquez G,Guo Q,Wang L,Brieba LG,Sousa R

    更新日期：2012-05-08 00:00:00

  • Structural consequences of cysteinylation of Cu/Zn-superoxide dismutase.

    abstract：:The metalloenzyme Cu/Zn-superoxide dismutase (SOD1) catalyzes the reduction of superoxide anions into molecular oxygen and hydrogen peroxide. Hydrogen peroxide can oxidize SOD1, resulting in aberrant protein conformational changes, disruption of SOD1 function, and DNA damage. Cells may have evolved mechanisms of regul...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi400613h

    authors： Auclair JR,Brodkin HR,D'Aquino JA,Petsko GA,Ringe D,Agar JN

    更新日期：2013-09-10 00:00:00

  • Role of lys100 in human dihydroorotate dehydrogenase: mutagenesis studies and chemical rescue by external amines.

    abstract：:Chemical modification, mutagenesis, chemical rescue, and isotope effect studies are used to identify and probe the roles of several conserved amino acid groups in catalysis by human dihydroorotate dehydrogenase. Time- and pH-dependent inactivation of human dihydroorotate dehydrogenase by trinitrobenzenesulfonate impli...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000630d

    authors： Jiang W,Locke G,Harpel MR,Copeland RA,Marcinkeviciene J

    更新日期：2000-07-11 00:00:00

  • Circular dichroism studies on the interaction of tryptophan synthase with pyridoxal 5'-phosphate.

    abstract：:The interaction between the beta 2 subunit of tryptophan synthase and the coenzyme pyridoxal 5'-phosphate (PLP) is characterized by induced circular dichroism (CD) in the near-UV (260-285 nm) and in the visible region (320-480 nm, extrinsic Cotton effect). Because of its high mean residue ellipticity ([theta] = 56 deg...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00525a017

    authors： Balk H,Merkl I,Bartholmes P

    更新日期：1981-10-27 00:00:00

  • Thermodynamics of the dimer-decamer transition of reduced human and plant 2-cys peroxiredoxin.

    abstract：:Isothermal titration calorimetry (ITC) is a powerful technique for investigating self-association processes of protein complexes and was expected to reveal quantitative data on peroxiredoxin oligomerization by directly measuring the thermodynamic parameters of dimer-dimer interaction. Recombinant classical 2-cysteine ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8002956

    authors： Barranco-Medina S,Kakorin S,Lázaro JJ,Dietz KJ

    更新日期：2008-07-08 00:00:00

  • In vitro reconstitution of the first steps of anatoxin-a biosynthesis in Oscillatoria PCC 6506: from free L-proline to acyl carrier protein bound dehydroproline.

    abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins in cyanobacteria and proposed a biosynthetic scheme starting from L-proline and involving three polyketide synthases for which the starte...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9018785

    authors： Méjean A,Mann S,Vassiliadis G,Lombard B,Loew D,Ploux O

    更新日期：2010-01-12 00:00:00

  • An inhibitor of ribosomal peptidyl transferase using transition-state analogy.

    abstract：:The phosphoramidate of CCdAp and puromycin (CCdApPuro) is a potent inhibitor of ribosomal peptidyl transferase, as assayed by the fragment reaction. Inhibition is competitive at the ribosomal A-site. CCdApPuro protects P-site-associated bases in the peptidyl transferase loop region of 23S rRNA from carbodiimide modifi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00002a001

    authors： Welch M,Chastang J,Yarus M

    更新日期：1995-01-17 00:00:00

  • Complementary DNA cloning and kinetic characterization of a novel intracellular serine proteinase inhibitor: mechanism of action with trypsin and factor Xa as model proteinases.

    abstract：:The full-length cDNA encoding a novel human intracellular serine proteinase inhibitor has been sequenced and found to encode a 376 amino acid protein (M(r) approximately 42.5K) that we designate as cytoplasmic antiproteinase. Analysis of the primary structure revealed that the cytoplasmic antiproteinase has the majori...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00177a037

    authors： Morgenstern KA,Sprecher C,Holth L,Foster D,Grant FJ,Ching A,Kisiel W

    更新日期：1994-03-22 00:00:00

  • Binding of the proline-rich segment of myelin basic protein to SH3 domains: spectroscopic, microarray, and modeling studies of ligand conformation and effects of posttranslational modifications.

    abstract：:Myelin basic protein (MBP) is a multifunctional protein involved in maintaining the stability and integrity of the myelin sheath by a variety of interactions with membranes and with cytoskeletal and other proteins. A central segment of MBP is highly conserved in mammals and consists of a membrane surface-associated am...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701336n

    authors： Polverini E,Rangaraj G,Libich DS,Boggs JM,Harauz G

    更新日期：2008-01-08 00:00:00

  • Fragment reconstitution of a small protein: folding energetics of the reconstituted immunoglobulin binding domain B1 of streptococcal protein G.

    abstract：:To elucidate early stages in protein folding, we have adopted a fragment reconstitution method for small proteins. This approach is expected to provide nuclei for protein folding and to allow us to investigate folding mechanisms. In previous work [Kobayashi, N., et al. (1995) FEBS Lett. 366, 99-103.] we demonstrated t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982271g

    authors： Honda S,Kobayashi N,Munekata E,Uedaira H

    更新日期：1999-01-26 00:00:00

  • PsbU provides a stable architecture for the oxygen-evolving system in cyanobacterial photosystem II.

    abstract：:PsbU is a lumenal peripheral protein in the photosystem II (PS II) complex of cyanobacteria and red algae. It is thought that PsbU is replaced functionally by PsbP or PsbQ in plant chloroplasts. After the discovery of PsbP and PsbQ homologues in cyanobacterial PS II [Thornton et al. (2004) Plant Cell 16, 2164-2175], w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047539k

    authors： Inoue-Kashino N,Kashino Y,Satoh K,Terashima I,Pakrasi HB

    更新日期：2005-09-13 00:00:00

  • Protonation state of Glu142 differs in the green- and blue-absorbing variants of proteorhodopsin.

    abstract：:Proteorhodopsins are a recently discovered class of microbial rhodopsins, ubiquitous in marine bacteria. Over 4000 variants have thus far been discovered, distributed throughout the oceans of the world. Most variants fall into one of two major groups, green- or blue-absorbing proteorhodopsin (GPR and BPR, respectively...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7018964

    authors： Kralj JM,Bergo VB,Amsden JJ,Spudich EN,Spudich JL,Rothschild KJ

    更新日期：2008-03-18 00:00:00

  • Interaction and localization of the retinitis pigmentosa protein RP2 and NSF in retinal photoreceptor cells.

    abstract：:RP2 is a ubiquitously expressed protein encoded by a gene associated with X-linked retinitis pigmentosa (XLRP), a retinal degenerative disease that causes severe vision loss. Previous in vitro studies have shown that RP2 binds to ADP ribosylation factor-like 3 (Arl3) and activates its intrinsic GTPase activity, but th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1005249

    authors： Holopainen JM,Cheng CL,Molday LL,Johal G,Coleman J,Dyka F,Hii T,Ahn J,Molday RS

    更新日期：2010-09-07 00:00:00

  • Folding of Aplysia limacina apomyoglobin involves an intermediate in common with other evolutionarily distant globins.

    abstract：:In the globin family, similarities in the folding mechanism have been found among different mammalian apomyoglobins (apoMb). The best-characterized intermediate of sperm whale apoMb, called I(AGH), is mainly stabilized by nativelike contacts among the A, G, and H helices involving a cluster of hydrophobic residues tha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035319l

    authors： Musto R,Bigotti MG,Travaglini-Allocatelli C,Brunori M,Cutruzzolà F

    更新日期：2004-01-13 00:00:00

  • Phospholipase A2 engineering. Structural and functional roles of the highly conserved active site residue aspartate-49.

    abstract：:Site-directed mutagenesis and high-resolution two-dimensional (2D) proton nuclear magnetic resonance (NMR) were used to probe the structural and functional roles of a highly conserved residue, Asp-49, in the interfacial catalysis by bovine pancreatic phospholipase A2 (PLA2, overexpressed in Escherichia coli). Accordin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00253a009

    authors： Li Y,Yu BZ,Zhu H,Jain MK,Tsai MD

    更新日期：1994-12-13 00:00:00