Abstract:
:Gp is a major GTP-binding protein of human placenta and platelets [Evans, T., Brown, M. L., Fraser, E. D., & Northup, J. K. (1986) J. Biol. Chem. 261, 7052-7059]. High-affinity guanine nucleotide binding is associated with a polypeptide migrating identically with H-ras on SDS-PAGE. We have characterized the interactions of preparations of purified human placental Gp with guanine nucleotides in detergent solution. Equilibrium binding studies with [35S]GTP gamma S, [3H]Gpp(NH)p, and [3H]GTP identified a single class of sites with a dissociation constant of 10 +/- 1, 153 +/- 61, and 125 +/- 77 nM for the ligands, respectively. These three ligands were mutually competitive with Ki values consistent with the Kd values from direct binding experiments. Competition for the binding of [3H]Gpp(NH)p was used to determine the specificity of the site. Ki values determined from this assay were 14 nM for GTP gamma S, 143 nM for Gpp(NH)p, 3.3 microM for GDP beta S, 69 nM for GTP, and 64 nM for GDP. ATP, ADP, cAMP, cGMP, and NAD+ had no detectable affinity for this site. While the equilibrium binding data fit well to a single class of sites, association kinetics of these ligands were better fit to two rate constants. Dissociation kinetics, however, were not clearly resolved into two rates.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Tamir A,Fawzi AB,Northup JKdoi
10.1021/bi00482a002subject
Has Abstractpub_date
1990-07-31 00:00:00pages
6947-54issue
30eissn
0006-2960issn
1520-4995journal_volume
29pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Tafazzin is the mitochondrial enzyme that catalyzes transacylation between a phospholipid and a lysophospholipid in remodeling. Mutations in tafazzin cause Barth syndrome, a potentially life-threatening disease with the major symptom being cardiomyopathy. In the tafazzin-deficient heart, cardiolipin (CL) acyl chains b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00042
更新日期:2018-04-10 00:00:00
abstract::Nogo-66 receptor (NgR1) is a leucine-rich repeat (LRR) protein that forms part of a signaling complex modulating axon regeneration. Previous studies have shown that the entire LRR region of NgR1, including the C-terminal cap of the LRR, LRRCT, is needed for ligand binding, and that the adjacent C-terminal region (CT s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0517483
更新日期:2005-12-20 00:00:00
abstract::Tyramine β-monooxygenase (TβM), the insect homologue of dopamine β-monooxygenase, is a neuroregulatory enzyme that catalyzes the β-hydroxylation of tyramine to yield octopamine. Mutation of the methionine (Met) ligand to Cu(M) of TβM, Met471Cys, yielded a form of TβM that is catalytically active but susceptible to ina...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300456f
更新日期:2012-09-25 00:00:00
abstract::The non-typeable Haemophilus influenzae strain DH1 was isolated from a 25 year old male patient with Fisher syndrome, a postinfectious autoimmune condition characterized by the presence of anti-GQ1b IgG antibodies that target and initiate damage to peripheral nerves. DH1 was found to display an alphaNeuAc(2-8)alphaNeu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700685s
更新日期:2007-07-10 00:00:00
abstract::Annexin V (AxV) is a member of a family of proteins that exhibit functionally relevant Ca2+-dependent binding to anionic phospholipid membranes. Protein structure and stability as a function of Ca2+ and phospholipids was studied by bulk phase infrared (IR) spectroscopy and by IR reflection-absorption spectroscopy (IRR...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9819677
更新日期:1999-01-12 00:00:00
abstract::Escherichia coli non-heme-binding ferritin A (EcFtnA) is a spherical cagelike protein that is composed of 24 identical subunits. EcFtnA dissociates into 2-mers under acidic conditions and can reassemble into the native structure when the pH is increased. To understand how electrostatic interactions influence the assem...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b01197
更新日期:2016-01-26 00:00:00
abstract::Ammonia is an inhibitor of water oxidation and a structural analogue for substrate water, making it a valuable probe for the structural properties of the possible substrate-binding site on the oxygen-evolving complex (OEC) in photosystem II (PSII). By using the NH(3)-induced upshift of the 1365 cm(-)(1) IR mode in the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050030k
更新日期:2005-07-19 00:00:00
abstract::The binding of ethidium bromide, as monitored by fluorescence enhancement, to chromatin prepared by nuclease digestion has been compared with the binding of the dye to sheared chromatin. The nuclease preparation (native chromatin) is characterized by a high affinity region of the Scatchard plot (r = 0-0.025, K1 = 1 X ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00622a002
更新日期:1977-02-08 00:00:00
abstract::The protein tyrosine kinase, pp60(c-)(src), is involved in cellular signaling and is activated during mitosis and in various tumors. We have been employing cyclic decapeptides to identify the determinants for substrate binding and phosphorylation to develop inhibitors competitive with protein substrates of Src. A stru...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992994f
更新日期:2000-05-02 00:00:00
abstract::Familial British dementia (FBD) is a rare neurodegenerative disorder and shares features with Alzheimer's disease, including amyloid plaque deposits, neurofibrillary tangles, neuronal loss, and progressive dementia. Immunohistochemical and biochemical analysis of plaques and vascular amyloid of FBD brains revealed tha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002287i
更新日期:2001-03-27 00:00:00
abstract::In refolding of iso-2 cytochrome c from Saccharomyces cerevisiae, there are two slow folding reactions, tau 1a and tau 1b. The slower of the slow reactions, tau 1a = 100-200 s, is observed only by absorbance changes, while tau 1b (10-20-fold faster) is detected by fluorescence changes. The temperature dependence of th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00348a024
更新日期:1985-12-31 00:00:00
abstract::Detailed information on room-temperature structure and oxidation state of the Photosystem II (PS II) manganese complex is needed to put mechanistic considerations on solid grounds. Because previously this information had not been available, the tetranuclear manganese complex was investigated by X-ray absorption spectr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9924258
更新日期:2000-06-20 00:00:00
abstract::N-Acyl ethanolamines (NAEs) constitute a large and diverse class of signaling lipids that includes the endogenous cannabinoid anandamide. Like other lipid transmitters, NAEs are thought to be biosynthesized and degraded on-demand rather than being stored in vesicles prior to signaling. The identification of enzymes in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060163l
更新日期:2006-04-18 00:00:00
abstract::Melanoma growth stimulatory activity (MGSA) is a mitogenic protein secreted by Hs294T melanoma cells that corresponds to the polypeptide encoded by the human gro gene. The MGSA/gro cDNA has been expressed in mammalian cells and the secreted recombinant factor has been purified. Biochemical and biological characterizat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00496a011
更新日期:1990-11-06 00:00:00
abstract::We studied the binding characteristics of a synthetic 20-residue peptide to supported single planar bilayers of phosphatidylcholine, and the orientation of the peptide by Fourier-transform infrared spectroscopy with an attenuated total reflection method. This peptide, designed to resemble a putative fusion peptide of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952547+
更新日期:1996-04-16 00:00:00
abstract::The Escherichia coli siderophore enterobactin is assembled from 2,3-dihydroxybenzoate (2,3-DHB) and l-serine by the nonribosomal peptide synthetases EntB and EntF. The processive thiol-template strategy used can be sabotaged by EntB misacylation. Through in vitro kinetic analysis, we demonstrate two potential routes t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi802207t
更新日期:2009-01-27 00:00:00
abstract::Hemophores from Serratia marcescens (HasA(sm)) and Pseudomonas aeruginosa (HasA(p)) bind hemin between two loops, which harbor the axial ligands H32 and Y75. Hemin binding to the Y75 loop triggers closing of the H32 loop and enables binding of H32. Because Yersinia pestis HasA (HasA(yp)) presents a Gln at position 32,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400280z
更新日期:2013-04-23 00:00:00
abstract::Sulfation is a Golgi-specific modification of secretory proteins. We have characterized the proteins that are labeled with 35SO4 in cultures of rat hepatocytes and studied their transport to the medium. Analysis by polyacrylamide gel electrophoresis showed that of the five most heavily labeled proteins, four had well-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00435a069
更新日期:1989-05-02 00:00:00
abstract::The Escherichia coli L-arabinose-binding protein (ABP) participates as a specific receptor in the transport of L-arabinose, D-fucose, and D-galactose through the periplasmic space. The wild-type protein binds L-arabinose about 40 times more strongly than D-fucose. A mutation of the protein at position 108 (Met-->Leu) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00080a013
更新日期:1993-07-27 00:00:00
abstract::Pepstatin is a slow and tight-binding inhibitor of pepsin. Preincubating enzyme and inhibitor in H2O and in D2O in the absence of substrate generates an inverse solvent isotope effect of Dk = 0.69 +/- 0.06 on the apparent first-order rate constant for the decay in enzymatic activity. Proton inventory analysis of the i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00198a032
更新日期:1994-08-16 00:00:00
abstract::Aminoglycoside 2''-phosphotransferase IVa [APH(2'')-IVa] is a member of a family of bacterial enzymes responsible for medically relevant resistance to antibiotics. APH(2'')-IVa confers high-level resistance against several clinically used aminoglycoside antibiotics in various pathogenic Enterococcus species by phospho...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200747f
更新日期:2011-07-19 00:00:00
abstract::The modified Tanford-Kirkwood electrostatic theory (Shire et al., 1974a) was applied to ferrimyoglobins from the following animal species: sperm whale (Physeter catodon), horse, California grey whale (Eschrichtius gibbosus), harbor seal (Phoca vitulina), and California sea lion (Zalophus californianus). Computations w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00678a002
更新日期:1975-04-08 00:00:00
abstract::In halophilic archaea the photophobic response is mediated by the membrane-embedded 2:2 photoreceptor/-transducer complex SRII/HtrII, the latter being homologous to the bacterial chemoreceptors. Both systems bias the rotation direction of the flagellar motor via a two-component system coupled to an extended cytoplasmi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi501160q
更新日期:2015-01-20 00:00:00
abstract::Misaligned structures can result from strand slippage during DNA replication and, if not repaired, would lead to mutations. Previously, we showed that strand slippage can occur upon misincorporation of a dNTP opposite thymine and cytosine templates, resulting in a misaligned structure with a T- or C-bulge. The formati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9014049
更新日期:2009-12-08 00:00:00
abstract::The bacterium Caulobacter crescentus is known to attach irreversibly to underwater surfaces by utilizing an adhesive structure called the holdfast, which exhibits the greatest known adhesive strength of any organism. The very small size of the holdfast (∼400 nm wide and ∼40 nm high) has made direct chemical analysis d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00625
更新日期:2020-09-22 00:00:00
abstract::The folding of many globular proteins from the unfolded (U) to the native (N) state appears to be describable by a two-state N ↔ U model, which has led to the general belief that protein folding occurs in a highly cooperative manner. One reason for the widespread belief in "two-state folding" is that protein folding r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00698
更新日期:2018-10-09 00:00:00
abstract::To investigate a conformational change accompanying peptide binding to class II MHC proteins, we probed the structure of a soluble version of the human class II MHC protein HLA-DR1 in empty and peptide-loaded forms. Peptide binding induced a large decrease in the effective radius of the protein as determined by gel fi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi983048m
更新日期:1999-05-04 00:00:00
abstract::Factor XIII is the terminal enzyme of the clotting cascade. A cDNA sequence encoding human placental factor XIII was expressed in Saccharomyces cerevisiae with the yeast ADH2-4c promoter. Expression levels were a strong function of the noncoding flanking DNA content of the construction. When the terminal 3'-flanking n...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00459a028
更新日期:1990-02-20 00:00:00
abstract::AhpF, the flavoprotein reductase component of the Salmonella typhimurium alkyl hydroperoxide reductase system, catalyzes the reduction of an intersubunit disulfide bond in the peroxidatic active site of the system's other component, AhpC, a member of the peroxiredoxin family. Previous studies have shown that AhpF can ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002766h
更新日期:2001-04-03 00:00:00
abstract::Phospholipase A(2) (PLA(2)) (E. C. 3.1.1.4) is a common enzyme in the two-way cascade mechanism leading to the production of proinflammatory compounds known as eicosanoids. The binding of phospholipase A(2) to the membrane surface and hydrolysis of phospholipids are thought to involve the formation of a hydrophobic ch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035076x
更新日期:2003-10-14 00:00:00