A conformational change in the human major histocompatibility complex protein HLA-DR1 induced by peptide binding.

abstract：:When alpha,beta-unsaturated substrates bind to the active site of enoyl-CoA hydratase, large spectral changes can be observed [D'Ordine, R. L., et al. (1994) Biochemistry 33, 12635-12643]. The differences in the isotropic magnetic shieldings of the free and active site-bound forms of the carbonyl, alpha-, and beta-car...

journal_title：Biochemistry

authors： D'Ordine RL,Pawlak J,Bahnson BJ,Anderson VE

更新日期：2002-02-26 00:00:00

abstract：:Shaker potassium (K+) channels normally lack intrasubunit and intersubunit disulfide bonds. However, disulfide bonds are formed between Shaker subunits in intact cells exposed to oxidizing conditions. Upon electrophoresis under nonreducing conditions, intersubunit disulfide bond formation was detected by the presence ...

journal_title：Biochemistry

authors： Schulteis CT,Nagaya N,Papazian DM

更新日期：1996-09-17 00:00:00

abstract：:Nramp (natural resistance-associated macrophage protein) family members have been characterized in mammals, yeast, and bacteria as divalent metal ion/H(+) symporters. In previous work, a bioinformatic approach was used for the identification of residues that are conserved within the Nramp family [Haemig, H. A., and Br...

journal_title：Biochemistry

authors： Haemig HA,Moen PJ,Brooker RJ

更新日期：2010-06-08 00:00:00

abstract：:The homologous series of optically active short-chain phosphatidylethanolamines (PE) from dibutyryl-PE to dioctanoyl-PE was synthesized. In addition, two monomeric short-chain phospholipid analogues that are not degraded by phospholipase A2 (1,2-bis[(butylcarbamyl)oxy]-sn-glycero-3-phosphocholine and the corresponding...

journal_title：Biochemistry

authors： Plückthun A,Rohlfs R,Davidson FF,Dennis EA

更新日期：1985-07-16 00:00:00

abstract：:Tyramine β-monooxygenase (TβM), the insect homologue of dopamine β-monooxygenase, is a neuroregulatory enzyme that catalyzes the β-hydroxylation of tyramine to yield octopamine. Mutation of the methionine (Met) ligand to Cu(M) of TβM, Met471Cys, yielded a form of TβM that is catalytically active but susceptible to ina...

journal_title：Biochemistry

authors： Osborne RL,Zhu H,Iavarone AT,Hess CR,Klinman JP

更新日期：2012-09-25 00:00:00

abstract：:High-risk human papillomavirus E6 participates in tumorigenic progression, mainly by its ability to promote p53 degradation. HPV transcripts show a complex splicing pattern, where E6* is the most abundant transcript in high-risk HPV types, comprising the first 50 amino acids of E6. No structural or biochemical informa...

journal_title：Biochemistry

authors： Heer A,Alonso LG,de Prat-Gay G

更新日期：2011-03-01 00:00:00

abstract：:A high molecular weight inert molecule, poly(ethylene glycol) (PEG), or a soluble protein, ovalbumin, causes parallel bundles of actin filaments in a crystalline-like structure under physiological conditions of ionic compositions and pH. The bundle formation depends on the molecular weight of PEG, and a larger molecul...

journal_title：Biochemistry

authors： Suzuki A,Yamazaki M,Ito T

更新日期：1989-07-25 00:00:00

abstract：:Heparin was isolated from an unusually large human hemangioma that contained an elevated level of mast cells. Purification of multimilligram quantities of heparin from this tissue sample permitted a thorough examination of its structure and activity. Characterization of this human heparin included the following: one-d...

journal_title：Biochemistry

authors： Linhardt RJ,Ampofo SA,Fareed J,Hoppensteadt D,Mulliken JB,Folkman J

更新日期：1992-12-15 00:00:00

abstract：:Evidence for the NADH-modulated formation of a complex between alpha-glycerol-3-phosphate dehydrogenase and l-lactate dehydrogenase was reported [Yong, H., Thomas, G. A., and Peticolas, W. L. (1993) Biochemistry 32, 11124-11131]. This NADH-modulated association suggested a mechanism of potentially great importance to ...

journal_title：Biochemistry

authors： Lehoux EA,Baker SM,Kovina MV,Hays FA,Spivey HO

更新日期：2003-05-27 00:00:00

abstract：:β-2-Microglobulin (β2m) forms amyloid fibrils in the joints of patients undergoing dialysis treatment as a result of kidney failure. One of the ways in which β2m can be induced to form amyloid fibrils in vitro is via incubation with stoichiometric amounts of Cu(II). To better understand the structural changes caused b...

journal_title：Biochemistry

authors： Borotto NB,Zhang Z,Dong J,Burant B,Vachet RW

更新日期：2017-02-28 00:00:00

abstract：:Recombination kinetics of CO to protoheme after laser photolysis have been measured vs. temperature and viscosity. A 25-ns laser pulse was focused on the sample to produce an excitation rate of 10(9)/s per heme. This temporarily populates the heme-CO state of dissociated pairs which either separate or recombine on a p...

journal_title：Biochemistry

authors： Marden MC,Hazard ES 3rd,Gibson QH

更新日期：1986-05-20 00:00:00

abstract：:The folding and unfolding rates of the small protein, barstar, have been monitored using stopped-flow measurements of intrinsic tryptophan fluorescence at 25 degrees C, pH 8.5, and have been compared over a wide range of urea and guanidine hydrochloride (GdnHCl) concentrations. When the logarithms of the rates of fold...

journal_title：Biochemistry

authors： Sridevi K,Udgaonkar JB

更新日期：2002-02-05 00:00:00

abstract：:A human genomic DNA library was screened for the gene coding for the gamma chain of fibrinogen by using a human cDNA for the gamma chain as a hybridization probe. The gene was identified in three overlapping recombinant lambda bacteriophage, and its sequence, including the immediate 5' and 3' flanking regions, was det...

journal_title：Biochemistry

authors： Rixon MW,Chung DW,Davie EW

更新日期：1985-04-09 00:00:00

abstract：:Rabbit muscle lactate dehydrogenase (LDH) was coupled to Sepharose in such a way that each molecule is expected to be attached via only one subunit. Dissociation of the bound active enzyme by several methods all yielded immobilized subunit derivatives which were inactive. These derivatives were capable of regenerating...

journal_title：Biochemistry

authors： Chan WW,Mosbach K

更新日期：1976-09-21 00:00:00

abstract：:The influence of structural alterations to the estradiol-17 beta (E2) molecule on the induction of pS2 and Cathepsin D (Cath D) mRNAs has been examined by Northern analysis of RNA extracted from MCF-7 cells. Exposure of cultures to estratriene did not affect the level of expression of these estrogen-responsive genes. ...

journal_title：Biochemistry

authors： Pilat MJ,Hafner MS,Kral LG,Brooks SC

更新日期：1993-07-13 00:00:00

abstract：:The membrane-bound closed channel structure of helices 4 and 5 (Lys-406-Asp-446) of the colicin E1 channel domain was investigated by using a site-directed fluorescence labeling technique. A bimane probe was covalently attached to a cysteine residue in a series of single-cysteine mutant proteins to scan each helix in ...

journal_title：Biochemistry

authors： Ho D,Merrill AR

更新日期：2009-02-17 00:00:00

abstract：:The mechanism of binding of reduced 1-(2-carboxyphenylamino)-1-deoxyribulose 5-phosphate (rCdRP) to two different binding sites on the bifunctional enzyme is determined by kinetic studies, using temperature-jump and stopped-flow equipment with fluorescence detection. Two rapid binding processes and a comparatively slo...

journal_title：Biochemistry

authors： Cohn W,Kirschner K,Paul C

更新日期：1979-12-25 00:00:00

abstract：:The P22 tailspike endorhamnosidase confers the high specificity of bacteriophage P22 for some serogroups of Salmonella differing only slightly in their O-antigen polysaccharide. We used several biophysical methods to study the binding and hydrolysis of O-antigen fragments of different lengths by P22 tailspike protein....

journal_title：Biochemistry

authors： Baxa U,Weintraub A,Seckler R

更新日期：2020-12-29 00:00:00

abstract：:The functional role of distinct phospholipid subclasses and molecular species in modulating gramicidin-mediated K+ flux was characterized through quantification of changes in the fluorescence intensity of ion specific fluorescent probes encapsulated inside vesicles comprised of individual molecular species of plasmeny...

journal_title：Biochemistry

authors： Chen X,Gross RW

更新日期：1995-06-06 00:00:00

abstract：:Attenuated total reflection Fourier transform infrared (ATR-FTIR) difference spectroscopy has been performed on samples of bovine cytochrome c oxidase that have been deposited as a thin film on the surface of a silicon microprism. The technique has several advantages over transmission methods in terms of amount of mat...

journal_title：Biochemistry

authors： Rich PR,Breton J

更新日期：2002-01-22 00:00:00

abstract：:The temperature dependence of the reduction potential of the CuA site in carbon monoxide inhibited cytochrome c oxidase has been measured with a spectroelectrochemical method adapted to the relatively weak near-infrared absorption of this copper ion. These measurements, together with parallel measurements on the 604-n...

journal_title：Biochemistry

authors： Wang H,Blair DF,Ellis WR Jr,Gray HB,Chan SI

更新日期：1986-01-14 00:00:00

abstract：:The aminoacylase-1/metallopeptidase 20 (Acy1/M20) family features several l-aminoacylases useful in biocatalysis. Mammalian Acy1, in particular, has been applied in racemic resolution and reverse hydrolysis. Despite recent advances in our understanding of the active site architecture and functioning, determinants of A...

journal_title：Biochemistry

authors： Lindner HA,Alary A,Wilke M,Sulea T

更新日期：2008-04-08 00:00:00

abstract：:During the past decade, there has been much debate about the enormous catalytic rate enhancement observed in enzymatic reactions involving carbanion intermediates. Our recent theoretical study has demonstrated the importance of the short strong hydrogen bond (SSHB) in the enzymatic reactions. Nevertheless, other recen...

journal_title：Biochemistry

authors： Kim KS,Kim D,Lee JY,Tarakeshwar P,Oh KS

更新日期：2002-04-23 00:00:00

abstract：:Base excision repair (BER) is the major corrective pathway for most spontaneous, oxidative, and alkylation DNA base and sugar damage. X-ray cross-complementing 1 (XRCC1) has been suggested to function at nearly every step of this repair process, primarily through direct protein-protein interactions. Using whole cell e...

journal_title：Biochemistry

authors： Wong HK,Kim D,Hogue BA,McNeill DR,Wilson DM 3rd

更新日期：2005-11-01 00:00:00

abstract：:The ratio of the second-order rate constants for reduction of dihydroxyacetone phosphate (DHAP) and of the neutral truncated substrate glycolaldehyde (GLY) by glycerol 3-phosphate dehydrogenase (NAD (+), GPDH) saturated with NADH is (1.0 x 10 (6) M (-1) s (-1))/(8.7 x 10 (-3) M (-1) s (-1)) = 1.1 x 10 (8), which was u...

journal_title：Biochemistry

authors： Tsang WY,Amyes TL,Richard JP

更新日期：2008-04-22 00:00:00

abstract：:The disulfide bond location of a homodimeric peptide, prothoracicotropic hormone (PTTH) of the silkworm, Bombyx mori, was determined by a combination of partial reduction and sequence analysis of peptide fragments generated through a partial reduction of PTTH followed by alkylation and enzyme digestion. The partial re...

journal_title：Biochemistry

authors： Ishibashi J,Kataoka H,Isogai A,Kawakami A,Saegusa H,Yagi Y,Mizoguchi A,Ishizaki H,Suzuki A

更新日期：1994-05-17 00:00:00

abstract：:The first committed step of guanine nucleotide biosynthesis is the oxidation of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) catalyzed by IMP dehydrogenase. The reaction involves the reduction of NAD(+) with the formation of a covalent enzyme intermediate (E-XMP). Hydrolysis of E-XMP requires th...

journal_title：Biochemistry

authors： Guillén Schlippe YV,Hedstrom L

更新日期：2005-09-06 00:00:00

abstract：:Thermal perturbation of the dicluster ferredoxin from Acidianus ambivalens was investigated employing a toolbox of spectroscopic methods. FTIR and visible CD were used for assessing changes of the secondary structure and coarse alterations of the [3Fe4S] and [4Fe4S] cluster moieties, respectively. Fine details of the ...

journal_title：Biochemistry

authors： Todorovic S,Leal SS,Salgueiro CA,Zebger I,Hildebrandt P,Murgida DH,Gomes CM

更新日期：2007-09-18 00:00:00

abstract：:Primase DnaG is an essential bacterial enzyme that synthesizes short ribonucleotide primers required for chromosomal DNA replication. Inhibitors of DnaG can serve as leads for development of new antibacterials and biochemical probes. We recently developed a nonradioactive in vitro primase-pyrophosphatase assay to iden...

journal_title：Biochemistry

authors： Biswas T,Green KD,Garneau-Tsodikova S,Tsodikov OV

更新日期：2013-10-01 00:00:00

abstract：:The enzyme 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase (EC 4.1. 2.16) (KDO 8-P synthase) that catalyzes the condensation of D-arabinose 5-phosphate (A 5-P) with phosphoenolpyruvate (PEP) to give 3-deoxy-D-manno-octulosonic acid 8-phosphate (KDO 8-P) and inorganic phosphate (Pi) was inactivated by the thiol-m...

journal_title：Biochemistry

authors： Salleh HM,Patel MA,Woodard RW

更新日期：1996-07-09 00:00:00