Abstract：
:7,8-diaminopelargonic acid (DAPA) synthase (EC 2.6.1.62) is a pyridoxal phosphate (PLP)-dependent transaminase that catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form DAPA in the antepenultimate step in the biosynthesis of biotin. The wild-type enzyme has a steady-state kcat value of 0.013 s(-1), and the K(m) values for SAM and KAPA are 150 and <2 microM, respectively. The k(max) and apparent K(m) values for the half-reaction of the PLP form of the enzyme with SAM are 0.016 s(-1) and 300 microM, respectively, while those for the reaction with DAPA are 0.79 s(-1) and 1 microM. The R391A mutant enzyme exhibits near wild-type kinetic parameters in the reaction with SAM, while the apparent K(m) for DAPA is increased 180-fold. The 2.1 A crystal structure of the R391A mutant enzyme shows that the mutation does not significantly alter the structure. These results indicate that the conserved arginine residue is not required for binding the alpha-amino acid SAM, but it is important for recognition of DAPA.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Eliot AC,Sandmark J,Schneider G,Kirsch JFdoi
10.1021/bi026339asubject
Has Abstractpub_date
2002-10-22 00:00:00pages
12582-9issue
42eissn
0006-2960issn
1520-4995pii
bi026339ajournal_volume
41pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Lactone-hydrolyzing enzymes derived from some Bacillus species are capable of disrupting quorum sensing in bacteria that use N-acyl-l-homoserine lactones (AHLs) as intercellular signaling molecules. Despite the promise of these quorum-quenching enzymes as therapeutic and anti-biofouling agents, the ring opening mechan...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061238o
更新日期：2006-11-07 00:00:00
abstract：:Escherichia coli class Ib ribonucleotide reductase (RNR) converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates in iron-limited and oxidative stress conditions. We have recently demonstrated in vitro that this RNR is active with both diferric-tyrosyl radical (Fe(III)(2)-Y(•)) and dimanganese(III)-Y(•) ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101881d
更新日期：2011-03-15 00:00:00
abstract：:cGMP is the natural activator of the cyclic nucleotide-gated channel originally isolated from rod photoreceptors but now known to be expressed in a wide variety of neural and non-neural cells. To identify antagonists of cGMP action and to better understand the interaction between cGMP and the channel protein, experime...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961763v
更新日期：1996-12-24 00:00:00
abstract：:DNA polymerases slide on DNA during replication, and the interface must be mobile for various conformational changes. The role of lubricant interfacial water is not understood. In this report, we systematically characterize the water dynamics at the interface and in the active site of a tight binding polymerase (pol β...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500810a
更新日期：2014-08-26 00:00:00
abstract：:The effect of mutating Gly 478 to Ala in rat cytochrome P450 2B1 on the metabolism of N-benzyl-1-aminobenzotriazole was investigated. The 7-ethoxy-4-(trifluoromethyl)coumarin O-deethylation activity of the wild-type enzyme was completely inactivated by incubating with 1 microM BBT. The G478A mutant, however, was not i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971064y
更新日期：1997-09-30 00:00:00
abstract：:X-ray absorption spectra are reported for the multi-Cu oxidase Rhus vernicifera laccase in oxidized and fully reduced forms and for laccase from which the type 2 Cu has been depleted (T2D). The structure of the Cu K edge for both preparations shows the presence of CuII and CuI in the oxidized and reduced states, respe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00310a008
更新日期：1984-07-17 00:00:00
abstract：:Protein serine/threonine phosphatase 2A (PP2A) is a critical regulator of numerous cellular signaling processes and a potential target for reactive electrophiles that dysregulate phosphorylation-dependent signal transduction cascades. The predominant cellular form of PP2A is a heterotrimeric holoenzyme consisting of a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060551n
更新日期：2006-08-22 00:00:00
abstract：:Electron transfer between the Rieske iron-sulfur protein (Fe(2)S(2)) and cytochrome c(1) was studied using the ruthenium dimer, Ru(2)D, to either photoreduce or photooxidize cytochrome c(1) within 1 micros. Ru(2)D has a charge of +4, which allows it to bind with high affinity to the cytochrome bc(1) complex. Flash pho...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000003o
更新日期：2000-04-18 00:00:00
abstract：:Histone H10 differs substantially from H1 in a 37-residue region of the primary structure that is conserved between the usual H1 histones of fish and mammals. Instead, H10 is homologous to H5 in this region. Histones H10 and H5 might be classified together in a histone category distinct from the usual H1. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00511a035
更新日期：1981-04-14 00:00:00
abstract：:Atrazine chlorohydrolase (AtzA) from Pseudomonas sp. ADP initiates the metabolism of the herbicide atrazine by catalyzing a hydrolytic dechlorination reaction to produce hydroxyatrazine. Sequence analysis revealed AtzA to be homologous to metalloenzymes within the amidohydrolase protein superfamily. AtzA activity was ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi020415s
更新日期：2002-12-03 00:00:00
abstract：:A novel cationic lipid was separated from bovine brain white matter by a series of chromatographies on carboxymethyl-Sephadex and silica gel in chloroform and methanol. Its structure was identified unambiguously as de-N-acetyllactotriaosylceramide (deNAcLc(3)Cer) by mass spectrometry and (1)H NMR. The natural occurren...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0504411
更新日期：2005-07-12 00:00:00
abstract：:The orientation of the myosin neck was monitored using electron paramagnetic resonance (EPR) spectroscopy. Gizzard regulatory light chain was labeled with a nitroxide spin probe and exchanged for the native subunit, located in the myosin neck, in rabbit psoas muscle fibers. The EPR spectra of rigor fibers indicated a ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960505v
更新日期：1996-08-06 00:00:00
abstract：:The nonconvertible substrate analogues di-, tri-, and tetramethylammonium are bound with fairly high affinity to oxidized methylamine dehydrogenase (MADHox) from Thiobacillus versutus and induce the same red-shift in the optical absorbance spectrum of MADHox as do the monovalent cations Cs+, Rb+, and NH4+. Like the mo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00040a002
更新日期：1995-10-10 00:00:00
abstract：:The last gene in the nuo operon of Escherichia coli, nuoN, encodes a membrane-bound subunit of Complex I (NADH:ubiquinone oxidoreductase). In this report, the gene for subunit N was disrupted by a 163 bp deletion in the chromosome, resulting in the loss of Complex I function, as measured by deamino-NADH oxidase activi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0340346
更新日期：2003-05-06 00:00:00
abstract：:A high degree of selectivity toward the target site of the pest organism is a desirable attribute for new safer agrochemicals. To assist in the design of novel herbicides, we determined the crystal structures of the herbicidal target enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD; EC 1.13.11.27) from the plant Arabi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049323o
更新日期：2004-08-17 00:00:00
abstract：:The preferential in vitro methylation of histone H3 by coactivator-associated arginine methyltransferase 1 (CARM1) has been proposed as a basis for its ability to enhance gene transcription [Chen, D., et al. (1999) Science 284, 2174-2177]. To further evaluate the significance of H3 methylation, we studied the kinetics...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002631b
更新日期：2001-05-15 00:00:00
abstract：:The role of acetylation on the conformation and association state of the inner core histone octamer isolated from HeLa cells was examined. Preparation of suitable quantities of pure acetylated and control inner core histones from HeLa cells required the development of a new preparative procedure. The results from size...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00384a041
更新日期：1987-05-19 00:00:00
abstract：:We have employed a combination of gel retardation, protein-DNA cross-linking, and protein-protein cross-linking techniques to further examine the 2,3,7,8-tetrachlorodibenzo-p- dioxin-(TCDD-) dependent changes in the Ah receptor that result in a DNA-binding conformation. Gel retardation analysis of DNA-Sepharose chroma...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00225a026
更新日期：1991-03-19 00:00:00
abstract：:The septins are a conserved family of guanosine-5'-triphosphate (GTP)-binding proteins. In mammals they are involved in a variety of cellular processes, such as cytokinesis, exocytosis, and vesicle trafficking. Specifically, SEPT4 has also been shown to be expressed in both human colorectal cancer and malignant melano...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061549z
更新日期：2006-11-21 00:00:00
abstract：:Reconstitution of highly purified aquaporin CHIP (channel-forming integral protein) into proteoliposomes was previously shown to confer high osmotic water permeability (Pf) to the membranes [Zeidel et al. (1992) Biochemistry 31, 7436-7440]. Here we report detailed ultrastructural, pharmacologic, and transport studies ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00172a042
更新日期：1994-02-15 00:00:00
abstract：:Specific interactions of human melanocortin-4 receptor (hMC4R) with its nonpeptide and peptide agonists were studied using alanine-scanning mutagenesis. The binding affinities and potencies of two synthetic, small-molecule agonists (THIQ, MB243) were strongly affected by substitutions in transmembrane alpha-helices (T...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0501840
更新日期：2005-08-30 00:00:00
abstract：:We studied the urea-induced unfolding transition of staphylococcal nuclease (SNase) and its five proline mutants (P47A, P47T, P117G, P47T/P117G, and P47A/P117G) [corrected] by peptide and aromatic circular dichroism and aromatic absorption spectroscopy at equilibrium and the refolding-unfolding kinetics of the protein...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963174v
更新日期：1997-05-27 00:00:00
abstract：:Silk fibroin exists in a number of different states, such as silk I and silk II, with different properties largely defined by differences in secondary structure composition. Numerous attempts have been made to control the transitions from silk I to silk II in vitro to produce high-performance materials. Of all the fac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049344i
更新日期：2004-09-07 00:00:00
abstract：:DNA-binding geometry and dynamics of a number of anthracyclines, including adriamycin and 4-demethoxydaunorubicin, interacting with DNA have been studied by means of linear dichroism and fluorescence techniques. The anthracycline chromophore is found to be approximately parallel to the plane of the DNA bases and to ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00421a024
更新日期：1988-10-18 00:00:00
abstract：:The reaction of Pseudomonas fluorescens kynureninase with L-kynurenine and L-alanine has been examined using rapid-scanning stopped-flow spectrophotometry. Mixing kynureninase with 0.5 mM L-kynurenine results in formation of a quinonoid intermediate, with lambdamax = 494 nm, within the dead time (ca. 2 ms) of the stop...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980066v
更新日期：1998-06-16 00:00:00
abstract：:Isothermal titration calorimetry (ITC) measurements of the binding 1-beta carbohydrate-substituted galactopyranoside derivatives to galectin-1 from bovine spleen, a dimer with one binding site per subunit, were performed at 283-285 and 298 K. The disaccharides were lactose, methyl beta-lactoside, lactulose, 4-O-beta-D...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9716478
更新日期：1998-04-28 00:00:00
abstract：:In an irreversible enzyme-catalyzed reaction, strong binding of the products would lead to substantial product inhibition. The X-ray crystal structure of the product complex of thymidylate synthase (1.83-A resolution, R factor = 0.183 for all data between 7.0 and 1.83 A) identifies a bound water molecule that serves t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00172a029
更新日期：1994-02-15 00:00:00
abstract：:Apurinic/apyrimidinic endonuclease 1 (APE1) is an Mg²⁺-dependent enzyme responsible for incising the DNA backbone 5' to an apurinic/apyrimidinic (AP) site. Here, we use rapid quench flow (RQF) techniques to provide a comprehensive kinetic analysis of the strand-incision activity (k(chemistry)) of APE1 acting on an aut...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401218r
更新日期：2013-10-29 00:00:00
abstract：:RSC (remodel the structure of chromatin) is an essential chromatin remodeler of Saccharomyces cerevisiae that has been shown to have DNA translocase properties. We studied the DNA binding properties of a "trimeric minimal RSC" (RSCt) of the RSC chromatin remodeling complex and the effect of nucleotides on this interac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200837b
更新日期：2011-09-20 00:00:00
abstract：:Oligodeoxyribonucleotides 8-12 nucleotides in length whose sequences are complementary to the 5' end, the initiation codon regions, or the coding regions of rabbit globin mRNA were tested for their ability to inhibit translation in a rabbit reticulocyte lysate and in a wheat germ extract. The oligomers interact specif...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00343a015
更新日期：1985-10-22 00:00:00
