Amino acid sequence of the nucleotide binding region of chloroplast coupling factor 1.

abstract：:Proteins performing multiple biochemical functions are called "moonlighting proteins" or extreme multifunctional (EMF) proteins. Mitochondrial cytochrome c is an EMF protein that binds multiple partner proteins to act as a signaling molecule, transfers electrons in the respiratory chain, and acts as a peroxidase in ap...

journal_title：Biochemistry

authors： Deacon OM,Karsisiotis AI,Moreno-Chicano T,Hough MA,Macdonald C,Blumenschein TMA,Wilson MT,Moore GR,Worrall JAR

更新日期：2017-11-21 00:00:00

abstract：:High-affinity mu-conotoxin block of skeletal muscle Na+ channels depends on an arginine at position 13 (Arg-13). To understand both the mechanism of toxin interaction and the general structure of its binding site in the channel mouth, we examined by thermodynamic mutant cycle analysis the interaction between the criti...

journal_title：Biochemistry

authors： Chang NS,French RJ,Lipkind GM,Fozzard HA,Dudley S Jr

更新日期：1998-03-31 00:00:00

abstract：:Protein tyrosine kinases (PTKs) are important regulators of mammalian cell function and their own activities are tightly regulated. Underlying their tight regulation, all PTKs contain multiple regulatory domains in addition to a catalytic domain. C-terminal Src kinase (Csk) contains a catalytic domain and a regulatory...

journal_title：Biochemistry

authors： Lin X,Ayrapetov MK,Lee S,Parang K,Sun G

更新日期：2005-02-08 00:00:00

abstract：:Cytochrome P450 3A4 (CYP3A4) inhibitors ritonavir and cobicistat, currently administered to HIV patients as pharmacoenhancers, were designed on the basis of the chemical structure/activity relationships rather than the CYP3A4 crystal structure. To better understand the structural basis for CYP3A4 inhibition and the li...

journal_title：Biochemistry

authors： Sevrioukova IF,Poulos TL

更新日期：2013-07-02 00:00:00

abstract：:Escherichia coli DNA photolyase contains a stable flavin radical that is readily photoreduced in the presence of added electron donors. Picosecond, nanosecond, and conventional flash photolysis technique have been employed to investigate the events leading to photoreduction from 40 ps to tens of milliseconds following...

journal_title：Biochemistry

authors： Heelis PF,Okamura T,Sancar A

更新日期：1990-06-19 00:00:00

abstract：:Inwardly rectifying potassium channels are important in cellular repolarization of many excitable tissues. Amino acid sequence alignment of different mammalian inward rectifier K(+) channels revealed two absolutely conserved cysteine residues in the putative extracellular face, suggesting a possible disulfide bond. Re...

journal_title：Biochemistry

authors： Cho HC,Tsushima RG,Nguyen TT,Guy HR,Backx PH

更新日期：2000-04-25 00:00:00

abstract：:The laminin alpha3 chain is mainly expressed at the skin, and its C-terminal G domain has a critical role in multiple biological functions. We screened for biologically active sites on the mouse laminin alpha3 chain G domain using 107 synthetic peptides on coated plates and conjugated to Sepharose beads with HT1080 hu...

journal_title：Biochemistry

authors： Urushibata S,Katagiri F,Takaki S,Yamada Y,Fujimori C,Hozumi K,Kikkawa Y,Kadoya Y,Nomizu M

更新日期：2009-11-10 00:00:00

abstract：:Phospholipase A(2) (PLA(2)) enzymes become activated by binding to biological membranes and hydrolyze phospholipids to free fatty acids and lyso-phospholipids, the precursors of inflammatory mediators. To understand the functional significance of amino acid residues at key positions, we have studied the effects of the...

journal_title：Biochemistry

authors： Nemec KN,Pande AH,Qin S,Bieber Urbauer RJ,Tan S,Moe D,Tatulian SA

更新日期：2006-10-17 00:00:00

abstract：:The carbon-13 (13C) nuclear magnetic resonance (NMR) behavior of the hyperactive analogue of luteinizing-hormone-releasing hormone (LH-RH), des-Gly-NH2(10)-[D-Leu6]LH-RH ethylamide, has been studied. The spectra are compared with those of the hypoactive des-Gly-NH2(10)-[L-Leu6]LH-RH ethylamide. Spin-lattice relaxation...

journal_title：Biochemistry

authors： Deslauriers R,Komoroski RA,Levy GC,Seely JH,Smith IC

更新日期：1976-10-19 00:00:00

abstract：:The transfer of cytochrome P-450 from bovine adrenocortical submitochondrial particles (smp) to unilamellar liposome membranes was investigated using a table top ultracentrifuge. Submitochondrial particles were incubated with liposome membranes at 25 degrees C and precipitated by ultracentrifugation at 200000g for a f...

journal_title：Biochemistry

authors： Kominami S,Onizuka M,Tasaka-Marumoto C,Takemori S

更新日期：1995-04-11 00:00:00

abstract：:The trans-aconitate methyltransferase from the bacterium Escherichia coli catalyzes the monomethyl esterification of trans-aconitate and related compounds. Using two-dimensional (1)H/(13)C nuclear magnetic resonance spectroscopy, we show that the methylation is specific to one of the three carboxyl groups and further ...

journal_title：Biochemistry

authors： Cai H,Strouse J,Dumlao D,Jung ME,Clarke S

更新日期：2001-02-20 00:00:00

abstract：:The involvement of calmodulin in the proliferation of Chinese hamster embryo fibroblast cells has been studied with a specific monoclonal antibody to calmodulin. We observed that calmodulin levels increase 2-fold in the late G1 period in these cells, and this coincides with the increase in DNA polymerase alpha activit...

journal_title：Biochemistry

authors： Reddy GP,Reed WC,Sheehan E,Sacks DB

更新日期：1992-11-03 00:00:00

abstract：:P450cam from Pseudomonas putida is the best characterized member of the vast family of cytochrome P450s, and it has long been believed to have a more rigid and closed active site relative to other P450s. Here we report X-ray structures of P450cam crystallized in the absence of substrate and at high and low [K(+)]. The...

journal_title：Biochemistry

authors： Lee YT,Wilson RF,Rupniewski I,Goodin DB

更新日期：2010-04-27 00:00:00

abstract：:βγ-Crystallins are important constituents of the vertebrate eye lens, whereas in microbes, they are prevalent as Ca2+-binding proteins. In archaea, βγ-crystallins are conspicuously confined to two methanogens, viz., Methanosaeta and Methanosarcina. One of these, i.e., M-crystallin from Methanosarcina acetivorans, has ...

journal_title：Biochemistry

authors： Srivastava SS,Jamkhindikar AA,Raman R,Jobby MK,Chadalawada S,Sankaranarayanan R,Sharma Y

更新日期：2017-03-07 00:00:00

abstract：:Molecular chaperones facilitate the folding and assembly of proteins and inhibit their aberrant aggregation. They thus offer several opportunities for biomedical and biotechnological applications, as for example they can often prevent protein aggregation more effectively than other therapeutic molecules, including sma...

journal_title：Biochemistry

authors： Aprile FA,Sormanni P,Vendruscolo M

更新日期：2015-08-18 00:00:00

abstract：:The manganese transport regulator (MntR) of Bacillus subtilis is activated by Mn(2+) to repress transcription of genes encoding transporters involved in the uptake of manganese. MntR is also strongly activated by cadmium, both in vivo and in vitro, but it is poorly activated by other metal cations, including calcium a...

journal_title：Biochemistry

authors： Kliegman JI,Griner SL,Helmann JD,Brennan RG,Glasfeld A

更新日期：2006-03-21 00:00:00

abstract：:The role of pyridoxal 5'-phosphate (PLP) in the radical-mediated amino group migration catalyzed by lysine 2,3-aminomutase from Clostridia SB4 has been investigated by electron spin echo envelope modulation (ESEEM) spectroscopy. This pulsed electron paramagnetic resonance (EPR) method was used to estimate the distance...

journal_title：Biochemistry

authors： Ballinger MD,Frey PA,Reed GH,LoBrutto R

更新日期：1995-08-08 00:00:00

abstract：:Type I protein arginine methyltransferases (PRMTs) catalyze asymmetric dimethylation of various proteins, and their dysregulations often correlate with tumorigenesis or developmental deficiency. Recent studies have focused on the in vivo substrate identification and the enzyme mechanism with peptide substrates. Howeve...

journal_title：Biochemistry

authors： Lee WC,Lin WL,Matsui T,Chen ES,Wei TY,Lin WH,Hu H,Zheng YG,Tsai MD,Ho MC

更新日期：2015-12-29 00:00:00

abstract：:The bovine adrenodoxin gene gives rise to two species of mRNA differing only at their 5'-ends. The synthesis of these two types of mRNA in bovine adrenal cortical cells is regulated transcriptionally in part by ACTH via the action of cAMP. Examination of the 5'-end of the adrenodoxin gene revealed that each mRNA conta...

journal_title：Biochemistry

authors： Chen JY,Waterman MR

更新日期：1992-03-03 00:00:00

abstract：:The thermodynamics of L-tryptophan and operator DNA binding to the tryptophan repressor of Escherichia coli were analyzed by titration microcalorimetry and van't Hoff analysis of footprinting titrations, respectively. At 25 degrees C in 10 mM sodium phosphate, pH 7.6, and 0.1 M NaCl, the binding of L-tryptophan to the...

journal_title：Biochemistry

authors： Jin L,Yang J,Carey J

更新日期：1993-07-20 00:00:00

abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins as well as the in-vitro reconstitution of the first steps of this biosynthesis. We now report experimental evidence supporting the propos...

journal_title：Biochemistry

authors： Mann S,Lombard B,Loew D,Méjean A,Ploux O

更新日期：2011-08-23 00:00:00

abstract：:The protective effect of cyclic stable nitroxide free radicals, having SOD-like activity, against oxidative damage was studied by using Escherichia coli xthA DNA repair-deficient mutant hypersensitive to H2O2. Oxidative damage induced by H2O2 was assayed by monitoring cell survival. The metal chelator 1,10-phenanthrol...

journal_title：Biochemistry

authors： Samuni A,Godinger D,Aronovitch J,Russo A,Mitchell JB

更新日期：1991-01-15 00:00:00

abstract：:In prokaryotes, the first three enzymes in pyrimidine biosynthesis, carbamoyl phosphate synthetase (CPS), aspartate transcarbamoylase (ATC), and dihydroorotase (DHO), are commonly expressed separately and either function independently (Escherichia coli) or associate into multifunctional complexes (Aquifex aeolicus). I...

journal_title：Biochemistry

authors： Zhang P,Martin PD,Purcarea C,Vaishnav A,Brunzelle JS,Fernando R,Guy-Evans HI,Evans DR,Edwards BF

更新日期：2009-02-03 00:00:00

abstract：:Evidence for the NADH-modulated formation of a complex between alpha-glycerol-3-phosphate dehydrogenase and l-lactate dehydrogenase was reported [Yong, H., Thomas, G. A., and Peticolas, W. L. (1993) Biochemistry 32, 11124-11131]. This NADH-modulated association suggested a mechanism of potentially great importance to ...

journal_title：Biochemistry

authors： Lehoux EA,Baker SM,Kovina MV,Hays FA,Spivey HO

更新日期：2003-05-27 00:00:00

abstract：:Hepatic microsomal NADPH-cytochrome P-450 reductase was solubilized from rabbit liver microsomes in the presence of detergents and purified to homogeneity by column chromatography. The purified reductase had a molecular weight of 78 000 and contained 1 mol each of FAD and FMN per mol of enzyme. On reduction with NADPH...

journal_title：Biochemistry

authors： Iyanagi T,Anan FK,Imai Y,Mason HS

更新日期：1978-05-30 00:00:00

abstract：:Calmodulin (CaM), by mediating the stimulation of the activity of two adenylyl cyclases (ACs), plays a key role in integrating the cAMP and Ca(2+) signaling systems. These ACs, AC1 and AC8, by decoding discrete Ca(2+) signals can contribute to fine-tuning intracellular cAMP dynamics, particularly in neurons where they...

journal_title：Biochemistry

authors： Masada N,Schaks S,Jackson SE,Sinz A,Cooper DM

更新日期：2012-10-09 00:00:00

abstract：:Epigallocatechin-3-gallate (EGCG) is a catechin found in green tea that can inhibit the amyloid formation of a wide variety of proteins. EGCG's ability to prevent or redirect the amyloid formation of so many proteins may reflect a common mechanism of action, and thus, greater molecular-level insight into how it exerts...

journal_title：Biochemistry

authors： Marcinko TM,Drews T,Liu T,Vachet RW

更新日期：2020-03-17 00:00:00

abstract：:The binding of horse ferricytochrome c to yeast cytochrome c peroxidase at pH 6.0 in 8.7 mM phosphate buffer (0.0100 M ionic strength) is characterized by a small, unfavorable enthalpy change (+1.91 +/- 0.16 kcal mol-1) and a large, positive entropy change (+37 +/- 1 eu). The free energy of binding depends strongly up...

journal_title：Biochemistry

authors： Kresheck GC,Vitello LB,Erman JE

更新日期：1995-07-04 00:00:00

abstract：:Seipin is a transmembrane protein that resides in the endoplasmic reticulum and concentrates at junctions between the ER and cytosolic lipid droplets. Mutations in the human seipin gene, including the missense mutation A212P, lead to congenital generalized lipodystrophy (CGL), characterized by the lack of normal adipo...

journal_title：Biochemistry

authors： Binns D,Lee S,Hilton CL,Jiang QX,Goodman JM

更新日期：2010-12-21 00:00:00

abstract：:The kinetics of formation of the ADP-sensitive (EP) and ADP-insensitive (E*P) phosphoenzyme intermediates of the CaATPase in sarcoplasmic reticulum (SR) were investigated by means of the quenched-flow technique. At 21 degrees C, addition of saturating ADP to SR vesicles phosphorylated for 116 ms with 10 microM ATP gav...

journal_title：Biochemistry

authors： Froehlich JP,Heller PF

更新日期：1985-01-01 00:00:00