Abstract：
:From a series of 15N-resolved 3D ROESY-HMQC and 13C-resolved 3D NOESY-HMQC spectra of the FK506 binding protein (FKBP)/ascomycin complex in H2O, the locations of three tightly bound water molecules were identified. These waters are all buried within the interior of the complex and form an integral part of its structure via a network of hydrogen bonds. Water molecules in identical locations exhibiting a similar hydrogen bonding pattern were also observed in the X-ray crystal structures of FKBP/FK506 [Van Duyne, G. D., Standaert, R. F., Karplus, P. A., Schreiber, S. L., & Clardy, J. (1991) Science 252, 839-842] and FKBP/rapamycin [Van Duyne, G. D., Standaert, R. F., Schreiber, S. L., & Clardy, J. (1991) J. Am. Chem. Soc. 113, 7433-7434]. However, none of the surface waters observed in the X-ray structures were detected in the NMR experiments due to their fast exchange with bulk water. In order to examine the effects of the three internal water molecules on NMR structure determinations of the FKBP/ascomycin complex, two sets of NMR structures were calculated either with or without the waters. By including the three internal waters in the structure calculations, a decrease in the root mean square deviation and improved angular order parameters was observed for FKBP residues in the vicinity of the water molecules. In addition, subtle conformational differences were observed between NMR structures generated either with or without the waters.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Xu RX,Meadows RP,Fesik SWdoi
10.1021/bi00061a004subject
Has Abstractpub_date
1993-03-16 00:00:00pages
2473-80issue
10eissn
0006-2960issn
1520-4995journal_volume
32pub_type
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