Abstract：
:ATP, its nonhydrolyzable analogue, AMP-PNP, and albumin were found to promote the dissociation of rhodopsin kinase from rod outer segments (ROS) containing photoactivated-rhodopsin (Rho*). These features were embodied in a protocol for the recovery of rhodopsin kinase from incubations containing ROS which had been subjected to a wide range of treatments. It was found that the supernatants recovered from mixtures containing ATP, rhodopsin kinase, and photolyzed ROS membranes catalyzed a Rho*-independent peptide phosphorylation as well as dark-phosphorylation of rhodopsin. The activities of this activated kinase in the two aforementioned assays were 7-8% of the maximum intrinsic activity found in appropriate standard assays (i.e., light-stimulated phosphorylation of rhodopsin and Rho*-dependent peptide phosphorylation). The activated kinase reverted to its inactive resting-state in a time dependent fashion, giving a tau 1/2 of decay of approximately 2 min. The intrinsic activity of kinase as measured by the standard assay, however, remained constant during this decay period. No positive evidence was found to suggest that the interconversion activated kinase <--> inactive kinase occurred by a phosphorylation event. Cumulatively, the results show that the interaction of rhodopsin kinase.ATP complex with Rho* leads to the formation, presumably due to the reorganization of the protein structure, of a soluble active kinase species which reverts to the inactive resting state in a time-dependent fashion.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Dean KR,Akhtar Mdoi
10.1021/bi952480qsubject
Has Abstractpub_date
1996-05-14 00:00:00pages
6164-72issue
19eissn
0006-2960issn
1520-4995pii
bi952480qjournal_volume
35pub_type
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