Biosynthesis of a thiamin antivitamin in Clostridium botulinum.

abstract：:Ca(2+)/calmodulin-dependent protein kinase kinases (CaMKKs) phosphorylate and activate specific downstream protein kinases, including CaMKI, CaMKIV, and 5'-AMP-activated protein kinase, which mediates a variety of Ca(2+) signaling cascades. CaMKKs have been shown to undergo autophosphorylation, although their role in ...

journal_title：Biochemistry

authors： Tokumitsu H,Hatano N,Fujimoto T,Yurimoto S,Kobayashi R

更新日期：2011-09-27 00:00:00

abstract：:PI-SceI is a member of an unusual class of rare cutting homing endonucleases produced by an autocatalytic protein splicing from a precursor. To analyze the structural and functional domain organization of the endonuclease PI-SceI and to examine whether the DNA binding activity can be structurally separated from the ca...

journal_title：Biochemistry

authors： Pingoud V,Grindl W,Wende W,Thole H,Pingoud A

更新日期：1998-06-02 00:00:00

abstract：:Double-stranded complementary deoxyribonucleic acid (cDNA) transcribed in vitro from a partially pure myosin light-chain messenger ribonucleic acid (mRNA) of the chick embryonic heart was cloned in Escherichia coli strain chi 1776 by using the HindIII cleavage site in the plasmid pBR322. The insertion of essentially f...

journal_title：Biochemistry

authors： Arnold HH,Siddiqui MA

更新日期：1979-12-11 00:00:00

abstract：:Actin interaction with L-plastin, a plastin/fimbrins isoform of the alpha-actinin family of molecules, is poorly characterized, from the biochemical point of view. Besides, molecular modeling of the T-isoform has recently provided a complete model of interaction with filamentous actin [Volkmann, N., DeRosier, D., Mats...

journal_title：Biochemistry

authors： Lebart MC,Hubert F,Boiteau C,Ventéo S,Roustan C,Benyamin Y

更新日期：2004-03-09 00:00:00

abstract：:Two high molecular weight DNA polymerases, which we have designated delta I and delta II, have been purified from calf thymus tissue. Using Bio Rex-70, DEAE-Sephadex A-25, and DNA affinity resin chromatography followed by sucrose gradient sedimentation, we purified DNA polymerase delta I 1400-fold to a specific activi...

journal_title：Biochemistry

authors： Crute JJ,Wahl AF,Bambara RA

更新日期：1986-01-14 00:00:00

abstract：:Comparisons of membrane glycopeptides from baby hamster kidney fibroblasts (BHK21/C13) and a clone transformed by Rous sarcoma virus (C13/B4) were made by using cells metabolically labeled with radioactive D-glucose and L-fucose. Most of the glycopeptides were metabolically labeled with both the general and the specif...

journal_title：Biochemistry

authors： Glick MC

更新日期：1979-06-12 00:00:00

abstract：:The structure/function properties of functional groups in the leadzyme have been studied by assaying the activity of analog ribozymes generated by the systematic substitution of modified nucleotides in the internal loop region of the ribozyme. Guanosine analogs introduced at positions 4 and 7 occupied by guanosine in ...

journal_title：Biochemistry

authors： Chartrand P,Usman N,Cedergren R

更新日期：1997-03-18 00:00:00

abstract：:To characterize the roles played by Glu44 and Glu56 of cytochrome b5 in the formation of the electrostatic complex between cytochrome c and cytochrome b5, the Glu44, Glu56, or both sites were changed to alanine by site-directed mutagenesis. The influence of these two residues on the protein stability was probed by inv...

journal_title：Biochemistry

authors： Qian W,Sun YL,Wang YH,Zhuang JH,Xie Y,Huang ZX

更新日期：1998-10-06 00:00:00

abstract：:We have studied the properties and structures of a series of Escherichia coli RNA polymerase ternary complexes formed during the initial steps of RNA chain initiation and elongation. Five different templates were used that contained the bacteriophage T7 A1 promoter or the E. coli Tac or the lac UV5 promoter, as well a...

journal_title：Biochemistry

authors： Krummel B,Chamberlin MJ

更新日期：1989-09-19 00:00:00

abstract：:Human alkyladenine DNA glycosylase (AAG) locates and excises a wide variety of structurally diverse alkylated and oxidized purine lesions from DNA to initiate the base excision repair pathway. Recognition of a base lesion requires flipping of the damaged nucleotide into a relatively open active site pocket between two...

journal_title：Biochemistry

authors： Hendershot JM,Wolfe AE,O'Brien PJ

更新日期：2011-03-22 00:00:00

abstract：:A conformational transition is described for the polypeptide, gramicidin A, in which a dimer that forms a left-handed intertwined antiparallel helix is converted to a single-stranded amino terminus to amino terminus right-handed helix. The starting structure is determined here by solution NMR methods while reference i...

journal_title：Biochemistry

authors： Zhang Z,Pascal SM,Cross TA

更新日期：1992-09-22 00:00:00

abstract：:The mechanism of bovine intestinal 5'-nucleotide phosphodiesterase was investigated by determining kinetic constants of systematically varied substrates, with emphasis on esters of phosphonic acids (which have much higer Vmax values than conventional phosphodiester substrates), and by pre-steady-state kinetics using b...

journal_title：Biochemistry

authors： Kelly SJ,Butler LG

更新日期：1977-03-22 00:00:00

abstract：:Excitation energy transfer and trapping processes in an iron stress-induced supercomplex of photosystem I from the cyanobacterium Synechocystis sp. PCC6803 were studied by time-resolved absorption and fluorescence spectroscopy on femtosecond and picosecond time scales. The data provide evidence that the energy transfe...

journal_title：Biochemistry

authors： Melkozernov AN,Bibby TS,Lin S,Barber J,Blankenship RE

更新日期：2003-04-08 00:00:00

abstract：:The motion of the ethanolamine head group in unsonicated lipid bilayers above and below the phase transition is studied by means of deuterium and phosphorus magnetic resonance. For this purpose, dipalmitoyl-3-sn-phosphatidylethanolamine is selectively deuterated at the two ethanolamine carbon atoms. The deuterium quad...

journal_title：Biochemistry

authors： Seelig J,Gally H

更新日期：1976-11-30 00:00:00

abstract：:Solid-state NMR spectroscopy was employed to study the molecular dynamics of the colicin Ia channel domain in the soluble and membrane-bound states. In the soluble state, the protein executes small-amplitude librations (with root-mean-square angular fluctuations of 0-10 degrees ) in the backbone and larger-amplitude m...

journal_title：Biochemistry

authors： Huster D,Xiao L,Hong M

更新日期：2001-06-26 00:00:00

abstract：:Although the importance of interactions involving both the cytosolic and transmembrane regions of SecY and SecE has been documented, no information has been available for the physical contact sites of these translocase subunits in their cytosolic domains. We now carried out site-specific cross-linking experiments to i...

journal_title：Biochemistry

authors： Satoh Y,Mori H,Ito K

更新日期：2003-06-24 00:00:00

abstract：:Cytochrome bc(1), a key enzyme of biological energy conversion, generates or uses a proton motive force through the Q cycle that operates within the two chains of cofactors that embed two catalytic quinone oxidation/reduction sites, the Q(o) site and the Q(i) site. The Q(o) site relies on the joint action of two cofac...

journal_title：Biochemistry

authors： Borek A,Sarewicz M,Osyczka A

更新日期：2008-11-25 00:00:00

abstract：:To identify the specific component(s) in the target membrane involved in fusion of vesicular stomatitis virus (VSV), we examined the interaction of the virus with human erythrocyte membranes with asymmetric and symmetric bilayer distributions of phospholipids. Fusion was monitored spectrofluorometrically by the octade...

journal_title：Biochemistry

authors： Herrmann A,Clague MJ,Puri A,Morris SJ,Blumenthal R,Grimaldi S

更新日期：1990-05-01 00:00:00

abstract：:Saccharomyces cerevisiae myristoylCoA:protein N-myristoyltransferase (Nmt1p) is an essential enzyme that catalyzes the transfer of myristic acid (C14:0) from myristoylCoA to the N-terminus of cellular proteins with a variety of functions. Nmts from an assortment of species display remarkable in vivo specificity for th...

journal_title：Biochemistry

authors： Bhatnagar RS,Schall OF,Jackson-Machelski E,Sikorski JA,Devadas B,Gokel GW,Gordon JI

更新日期：1997-06-03 00:00:00

abstract：:Thermodynamic and structural evidence indicates that the DNA binding domains of lac repressor (lacI) exhibit significant conformational adaptability in operator binding, and that the marginally stable helix-turn-helix (HTH) recognition element is greatly stabilized by operator binding. Here we use circular dichroism a...

journal_title：Biochemistry

authors： Felitsky DJ,Record MT Jr

更新日期：2003-02-25 00:00:00

abstract：:From an evolutionary point of view, plant and animal ferritins arose from a common ancestor, but plant ferritin exhibits different features as compared with the animal analogue. One major difference is that the 4-fold channels naturally occurring in plant ferritin are hydrophilic, whereas the 4-fold channels in animal...

journal_title：Biochemistry

authors： Lv C,Zhang S,Zang J,Zhao G,Xu C

更新日期：2014-04-15 00:00:00

abstract：:The in vitro hydrolysis by porcine kidney prolidase of the imidodipeptide L-alanyl-L-proline was monitored by using 1H high-resolution NMR spectroscopy. The dipeptide exists as an equilibrium mixture of isomers with cis or trans conformation about the peptide bond. The 13C and 1H NMR spectra of the dipeptide displayed...

journal_title：Biochemistry

authors： King GF,Middlehurst CR,Kuchel PW

更新日期：1986-03-11 00:00:00

abstract：:We have investigated the effects of mixed Na+: Mg2+ ionic solutions on the stability of the nonspecific lac repressor-DNA complex. The effects of Mg2+ are simply interpreted in terms of its role as a competitor (with repressor) for DNA sites. From these studies, the binding constant of the Mg-DNA complex can be determ...

journal_title：Biochemistry

authors： Record MT Jr,deHaseth PL,Lohman TM

更新日期：1977-11-01 00:00:00

abstract：:The solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of alpha(1-3)-, alpha(1-2)-, beta(1-2)-, and beta(1-4)-linked units is described. The conformational properties of these glycopeptides were determined by high-resolut...

journal_title：Biochemistry

authors： Brisson JR,Carver JP

更新日期：1983-07-19 00:00:00

abstract：:The non-typeable Haemophilus influenzae strain DH1 was isolated from a 25 year old male patient with Fisher syndrome, a postinfectious autoimmune condition characterized by the presence of anti-GQ1b IgG antibodies that target and initiate damage to peripheral nerves. DH1 was found to display an alphaNeuAc(2-8)alphaNeu...

journal_title：Biochemistry

authors： Houliston RS,Koga M,Li J,Jarrell HC,Richards JC,Vitiazeva V,Schweda EK,Yuki N,Gilbert M

更新日期：2007-07-10 00:00:00

abstract：:The 5'-untranslated region of positive-strand RNA viruses harbors many cis-acting RNA structural elements that are important for various viral processes such as replication, translation, and packaging of new virions. Among these is loop B RNA of the stem-loop IV domain within the internal ribosomal entry site (IRES) o...

journal_title：Biochemistry

authors： Du Z,Ulyanov NB,Yu J,Andino R,James TL

更新日期：2004-05-18 00:00:00

abstract：:Glycogen phosphorylase is a muscle enzyme which metabolizes glycogen, producing glucose-1-phosphate, which can be used for the production of ATP. Phosphorylase activity is regulated by phosphorylation/dephosphorylation, and by the allosteric binding of numerous effectors. In this work, we have studied 10 site-directed...

journal_title：Biochemistry

authors： Biorn AC,Graves DJ

更新日期：2001-05-01 00:00:00

abstract：:A simple procedure is introduced for accurately defining the precision with which the Cartesian coordinates of any macromolecular structure are determined by nuclear Overhauser data. The method utilizes an ensemble of structures obtained from an array of independent simulated data sets derived from a final structure. ...

journal_title：Biochemistry

authors： Shriver J,Edmondson S

更新日期：1993-02-16 00:00:00

abstract：:Modified (Arg63-Ile64 reactive-site peptide bond hydrolyzed) soybean trypsin inhibitor (Kunitz) with all reactive amino groups, except that of Ile64, protected was described in the preceding paper (Kowalski, D., and Laskowski, M., Jr. (1976), Biochemistry, preceding paper in this issue). Treatment of this inhibitor wi...

journal_title：Biochemistry

authors： Kowalski D,Laskowski M Jr

更新日期：1976-03-23 00:00:00

abstract：:The quantitative tissue specificity of the high mobility group (HMG) chromosomal proteins was investigated. Perchloric acid (PCA) extracts of four different chicken tissues and erythrocytes contained three proteins which comigrated on NaDodSO4-polyacrylamide gels with the HMG's 1,2, and E from erythrocyte nuclei. Thes...

journal_title：Biochemistry

authors： Gordon JS,Rosenfeld BI,Kaufman R,Williams DL

更新日期：1980-09-16 00:00:00