当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Human alpha- to zeta-thrombin cleavage occurs with neutrophil cathepsin G or chymotrypsin while fibrinogen clotting activity is retained.

Human alpha- to zeta-thrombin cleavage occurs with neutrophil cathepsin G or chymotrypsin while fibrinogen clotting activity is retained.

Abstract：

:Human neutrophil cathepsin G or bovine chymotrypsin proteolytically cleaved human alpha-thrombin at the B-chain Trp148-Thr149 bond generating a new form, zeta-thrombin. While incubation of alpha-thrombin with cathepsin G at pH 7.4 and 37 degrees C resulted in a partial loss of fibrinogen clotting activity, 86 +/- 13% of the clotting activity and 99 +/- 16% of the active sites titratable with p-nitrophenyl p-guanidinobenzoate were retained upon controlled passage of alpha-thrombin through chymotrypsin-Sepharose 4B at pH 6.2 or 7.4 and 24 degrees C (n = 15). Kinetic parameters for H-D-hexahydrotyrosyl-Ala-Arg p-nitroanilide were Km = 1.52 +/- 0.60 vs 1.32 +/- 0.18 microM and kcat = 51.9 +/- 2.9 vs 35.8 +/- 6.4 s-1 with alpha-thrombin vs chymotrypsin-prepared zeta-thrombin (n = 4 vs 3), respectively (I = 0.15 M, pH 7.4, and 24 degrees C). Some 95% of the clotting activity was lost when zeta-thrombin was passed through trypsin-Sepharose 4B under conditions for converting alpha- to nonclotting beta- and subsequently gamma-thrombin. The resulting gamma-like thrombins eluted bimodally with 260 and 310 mM NaCl when applied to Amberlite CG-50 resin [cross-linked poly(methylacrylic acid)] developed with a linear salt gradient in 50 mM Tris at pH 7.4 and 24 degrees C. These elution peaks correspond to 240, 330, and 350 mM NaCl for gamma-, alpha-, and zeta-thrombin, respectfully, implying that the anion-binding exosite is partially destroyed in gamma-like thrombins but is intact in zeta-thrombin.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Brezniak DV,Brower MS,Witting JI,Walz DA,Fenton JW 2nd

doi

10.1021/bi00466a017

subject

Has Abstract

pub_date

1990-04-10 00:00:00

pages

3536-42

issue

14

eissn

0006-2960

issn

1520-4995

journal_volume

29

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Interfacial properties of the polyheme cytochrome c3 superfamily from Desulfovibrio.

    abstract：:In order to compare the interfacial behavior of the polyheme cytochromes c which belong to the cytochrome c3 superfamily, the monomolecular film technique was used to determine whether and how these metalloproteins interact with (phospho)lipids). Measurements of the variations of surface pressure and surface potential...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00036a005

    authors： Florens L,Ivanova M,Dolla A,Czjzek M,Haser R,Verger R,Bruschi M

    更新日期：1995-09-12 00:00:00

  • Conformational Closure of the Catalytic Site of Human CD38 Induced by Calcium.

    abstract：:First identified on the surface of lymphoids as a type II transmembrane protein, CD38 has now been established to have dual functions not only as a receptor but also as a multifunctional enzyme,catalyzing the synthesis of and hydrolysis of a general calcium messenger molecule, cyclic ADP-ribose(cADPR). The receptorial...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：

    authors： Liu Q,Graeff R,Kriksunov IA,Lam CM,Lee HC,Hao Q

    更新日期：2008-12-30 00:00:00

  • Peptidomics of prolyl endopeptidase in the central nervous system.

    abstract：:Prolyl endopeptidase (Prep) is a member of the prolyl peptidase family and is of interest because of its unique biochemistry and connections to cognitive function. Using an unbiased mass spectrometry (MS)-based peptidomics platform, we identified Prep-regulated peptides in the central nervous system (CNS) of mice by m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901637c

    authors： Nolte WM,Tagore DM,Lane WS,Saghatelian A

    更新日期：2009-12-22 00:00:00

  • An FHA phosphoprotein recognition domain mediates protein EmbR phosphorylation by PknH, a Ser/Thr protein kinase from Mycobacterium tuberculosis.

    abstract：:In bacteria, regulatory phosphorylation of proteins at serine and/or threonine residues by Ser/Thr protein kinase (STPK) is an emerging theme in prokaryotic signaling, particularly since the prediction of the occurrence of several STPKs from genome sequencing and sequence surveys. Here we show that protein PknH posses...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035150b

    authors： Molle V,Kremer L,Girard-Blanc C,Besra GS,Cozzone AJ,Prost JF

    更新日期：2003-12-30 00:00:00

  • The role of methionine-192 of the chymotrypsin active site in the binding and catalysis of mono(amino acid) and peptide substrates.

    abstract：:We find that specific oxidation for the Met-192 residue in delta-chymotrypsin to methionine sulfoxide results in a twofold increase in Km(app) and unchanged kcat in the hydrolysis of N-acetyl mono(amino acid) amide substrates. However, the catalyzed hydrolyses of N-acetyl dipeptide amide substrates by (methionine sulf...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00664a006

    authors： Treadway WJ Jr,Schultz RM

    更新日期：1976-09-21 00:00:00

  • Structural investigation of the binding of a herpesviral protein to the SH3 domain of tyrosine kinase Lck.

    abstract：:Herpesvirus saimiri codes for a tyrosine kinase interacting protein (Tip) that interacts with both the SH3 domain and the kinase domain of the T-cell-specific tyrosine kinase Lck via two separate motifs. The activation of Lck by Tip is considered as a key event in the transformation of human T-lymphocytes during herpe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015986j

    authors： Schweimer K,Hoffmann S,Bauer F,Friedrich U,Kardinal C,Feller SM,Biesinger B,Sticht H

    更新日期：2002-04-23 00:00:00

  • Molecular cloning of testicular 20 alpha-hydroxysteroid dehydrogenase: identity with aldose reductase.

    abstract：:Complementary DNA (cDNA) clones encoding bovine testicular 20 alpha-hydroxysteroid dehydrogenase (20 alpha-HSD) have been isolated from a bovine testicular lambda gt11 library using polyclonal antibodies against 20 alpha-HSD and DNA probe hybridization. Nucleotide sequencing of three independently isolated clones was ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00057a003

    authors： Warren JC,Murdock GL,Ma Y,Goodman SR,Zimmer WE

    更新日期：1993-02-16 00:00:00

  • Insights into the Mechanism of the Cyanobactin Heterocyclase Enzyme.

    abstract：:Cyanobactin heterocyclases share the same catalytic domain (YcaO) as heterocyclases/cyclodehydratases from other ribosomal peptide (RiPPs) biosynthetic pathways. These enzymes process multiple residues (Cys/Thr/Ser) within the same substrate. The processing of cysteine residues proceeds with a known order. We show the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00084

    authors： Ge Y,Czekster CM,Miller OK,Botting CH,Schwarz-Linek U,Naismith JH

    更新日期：2019-04-23 00:00:00

  • Phosphinothricin-tripeptide synthetases from Streptomyces viridochromogenes.

    abstract：:Phosphinothricyl-alanyl-alanine (Pt tripeptide (Ptt), bialaphos) is a metabolite produced by Streptomyces viridochromogenes and Streptomyces hygroscopicus. It contains the unique phosphinoamino acid phosphinothricin (Pt), which after cleavage from Ptt is active as an inhibitor of glutamine synthetase. We have isolated...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9719410

    authors： Grammel N,Schwartz D,Wohlleben W,Keller U

    更新日期：1998-02-10 00:00:00

  • Structural determination of oligosaccharides derived from lipooligosaccharide of Neisseria gonorrhoeae F62 by chemical, enzymatic, and two-dimensional NMR methods.

    abstract：:F62 LOS of Neisseria gonorrhoeae consists of two major LOS components; the higher and smaller molecular weight (MW) components were recognized by MAbs 1-1-M and 3F11 respectively. Base-line separation of the two major oligosaccharide (OS) components from F62 LOS was achieved by Bio-Gel P-4 chromatography after dephosp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00107a028

    authors： Yamasaki R,Bacon BE,Nasholds W,Schneider H,Griffiss JM

    更新日期：1991-10-29 00:00:00

  • Neutron structure of subtilisin BPN': effects of chemical environment on hydrogen-bonding geometries and the pattern of hydrogen-deuterium exchange in secondary structure elements.

    abstract：:The neutron structure of subtilisin BPN' has been refined and analyzed at 2.0-A resolution. The structure studied was a mutant variant of subtilisin, Met222----Gln, and was used because large, uninhibited crystals could be grown, which was not the case for the native molecule. Comparison of the structure with that of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00219a008

    authors： Kossiakoff AA,Ultsch M,White S,Eigenbrot C

    更新日期：1991-02-05 00:00:00

  • Inhibition of phosphomannose isomerase by mercury ions.

    abstract：:Mercury ions can inhibit Candida albicans phosphomannose isomerase (PMI) by two different processes at sub-micromolar concentrations. Kinetic studies show that mercury ions are in rapid equilibrium with the enzyme and cause a clear partial noncompetitive inhibition when mannose 6-phosphate is used as the substrate. Th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00190a018

    authors： Wells TN,Payton MA,Proudfoot AE

    更新日期：1994-06-21 00:00:00

  • Binding of metal cyanide complexes to bovine liver rhodanese in the crystalline state.

    abstract：:Bovine liver rhodanese, which catalyzes the transfer of sulfur atoms between a variety of sulfur donor and sulfur acceptor substrates, is inhibited by metal cyanide complexes [Volini, M., Van Sweringen, B., & Chen, F.-Sh. (1978) Arch. Biochem. Biophys. 191, 205-215]. Crystallographic studies are described which reveal...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00281a026

    authors： Lijk LJ,Kalk KH,Brandenburg NP,Hol WG

    更新日期：1983-06-07 00:00:00

  • A single-stranded DNA binding protein that specifically recognizes cis-acting sequences in the replication origin and transcriptional promoter region of Tetrahymena rDNA.

    abstract：:Type I repeat sequences are evolutionarily conserved sequence elements found in the replication origin and transcriptional promoter region of the rRNA genes (rDNA) in Tetrahymena thermophila. An abundant single-stranded DNA binding protein, ssA-TIBF, specifically interacts with the A-rich strand of the Type I repeat s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00014a011

    authors： Hou Z,Umthun AR,Dobbs DL

    更新日期：1995-04-11 00:00:00

  • A common catalytic mechanism for proteins of the HutI family.

    abstract：:Imidazolonepropionase (HutI) (imidazolone-5-propanote hydrolase, EC 3.5.2.7) is a member of the amidohydrolase superfamily and catalyzes the conversion of imidazolone-5-propanoate to N-formimino-L-glutamate in the histidine degradation pathway. We have determined the three-dimensional crystal structures of HutI from A...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800180g

    authors： Tyagi R,Eswaramoorthy S,Burley SK,Raushel FM,Swaminathan S

    更新日期：2008-05-20 00:00:00

  • Direct identification of the agonist binding site in the human brain cholecystokininB receptor.

    abstract：:In investigating the agonist binding site of the human brain cholecystokininB receptor (CCKBR), we employed the direct protein chemical approach using a photoreactive tritiated analogue of sulfated cholecystokinin octapeptide, which contains the p-benzoylbenzoyl moiety at the N-terminus, followed by purification of th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990269z

    authors： Anders J,Blüggel M,Meyer HE,Kühne R,ter Laak AM,Kojro E,Fahrenholz F

    更新日期：1999-05-11 00:00:00

  • Triplex formation of chemically modified homopyrimidine oligonucleotides: thermodynamic and kinetic studies.

    abstract：:We have investigated effects of chemical modifications of a third strand on the thermodynamic and kinetic properties of the triplex formation between a 23-bp duplex and each of four kinds of 15-mer chemically modified third strands using isothermal titration calorimetry and interaction analysis system. The chemical mo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990832d

    authors： Torigoe H,Shimizume R,Sarai A,Shindo H

    更新日期：1999-11-02 00:00:00

  • Inhibition of in vitro transcription by a triplex-forming oligonucleotide targeted to human c-myc P2 promoter.

    abstract：:Triplex-forming oligonucleotides (TFOs) have been shown to bind in a sequence-specific manner to polypurine/polypyrimidine sequences in several human gene promoters, including the c-myc P1 promoter. TFOs have been shown to inhibit transcription in vitro and the expression of target genes in cell culture. The human c-m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00025a023

    authors： Kim HG,Miller DM

    更新日期：1995-06-27 00:00:00

  • Smaller discoidal high-density lipoprotein particles form saddle surfaces, but not planar bilayers.

    abstract：:Discoidal high-density lipoprotein (HDL) particles are known to be fractionalized into several discrete populations in plasma and to differ in behavior according to size; however, their structural differences and the factors regulating their size are less understood. In this study, we prepared several reconstituted HD...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900785x

    authors： Miyazaki M,Nakano M,Fukuda M,Handa T

    更新日期：2009-08-18 00:00:00

  • Localization of a trifluoperazine binding site on troponin C.

    abstract：:Trifluoperazine (TFP) was shown to interact with the cyanogen bromide fragment 9 (CB9) (residues 84-135) of rabbit skeletal troponin C and with a synthetic peptide representing the N-terminal region of CB9. The phenothiazine did not affect the calcium binding property of CB9 as observed by proton magnetic resonance an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00276a010

    authors： Gariépy J,Hodges RS

    更新日期：1983-03-29 00:00:00

  • Benzothiazepine binding domain of purified L-type calcium channels: direct labeling using a novel fluorescent diltiazem analogue.

    abstract：:We have synthesized a series of N-propylamino-substituted benzazepinones (NPSBs) as specific probes for the benzothiazepinone (BTZ) binding domain of muscle L-type calcium channels (LTCCs). NPSBs were identified which possess high affinity for the channel after purification. We synthesized a fluorescent NPSB, DMBODIPY...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00010a039

    authors： Brauns T,Cai ZW,Kimball SD,Kang KC,Haugland RP,Berger W,Berjukov S,Hering S,Glossmann H,Striessnig J

    更新日期：1995-03-14 00:00:00

  • Purification, properties, and partial structure elucidation of a high-molecular-weight glycoprotein from cervical mucus of the bonnet monkey (Macaca radiata).

    abstract：:A high-molecular-weight glycoprotein has been purified from the cervical mucus of the bonnet monkey (Macaca radiata). The glycoprotein was shown to be homogeneous by electrophoresis, sedimentation equilibrium, and N-terminal group determination, and to contain 19% protein, 19% D-galactose, 18% N-acetyl-D-galactosamine...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00626a042

    authors： Hatcher VB,Schwarzmann GO,Jeanloz RW,McArthur JW

    更新日期：1977-04-05 00:00:00

  • Characterization of the basic amphiphilic alpha-helix calmodulin-binding domain of a 61.5 kDa tobacco calmodulin-binding protein.

    abstract：:A 19-amino acid residue peptide, Gly-Trp-Leu-Lys-Ile-Lys-Ala-Ala-Met-Arg-Trp-Gly-Phe-Phe-Val-Arg-Lys-Lys- Ala, corresponding to the basic amphiphilic alpha-helix (BAA) motif at the C-terminus of a recombinant tobacco calmodulin-binding protein, TCB60, was synthesized. The interaction of the synthetic binding domain wi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9621674

    authors： Dash S,Niemaczura W,Harrington HM

    更新日期：1997-02-25 00:00:00

  • Identification of the six ligands to manganese(II) in transition-state-analogue complexes of creatine kinase: oxygen-17 superhyperfine coupling from selectively labeled ligands.

    abstract：:The complete coordination scheme for Mn(II) in transition-state-analogue complexes with creatine kinase has been determined by electron paramagnetic resonance (EPR) spectroscopy. Perturbations in the EPR spectra for Mn(II) due to superhyperfine coupling to 17O of selectively labeled ligands have been used to identify ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00565a002

    authors： Reed GH,Leyh TS

    更新日期：1980-11-25 00:00:00

  • Energetics of peptide recognition by the second PDZ domain of human protein tyrosine phosphatase 1E.

    abstract：:Formation of protein-protein assemblies is essential in maintaining cell structure and function. Conservation of structural motifs and binding sites is the result of evolutionary pressure for solutions compatible with both molecular economy and regulation. PDZ domains are a typical example: A conserved fold governs sp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061869i

    authors： Milev S,Bjelić S,Georgiev O,Jelesarov I

    更新日期：2007-01-30 00:00:00

  • Reduction of ferrylmyoglobin and ferrylhemoglobin by nitric oxide: a protective mechanism against ferryl hemoprotein-induced oxidations.

    abstract：:The reactions of metmyoglobin (metMb) and methemoglobin (metHb), oxidized to their respective oxoferryl free radical species (.Mb-FeIV = O/.Hb-4FeIV = O) by tert-butyl hydroperoxide (t-BuOOH), with nitric oxide (NO.) were studied by a combination of optical, electron spin resonance (ESR), ionspray mass (MS), fluoresce...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00020a014

    authors： Gorbunov NV,Osipov AN,Day BW,Zayas-Rivera B,Kagan VE,Elsayed NM

    更新日期：1995-05-23 00:00:00

  • The 68 kDa calmodulin-binding protein is tightly associated with the multiprotein DNA polymerase alpha-primase complex in HeLa cells.

    abstract：:Calcium and its receptor protein calmodulin function in the regulation of proliferation of mammalian cells. A 68 kDa calmodulin-specific binding protein was shown previously to be associated with growth factor-dependent progression of a variety of mammalian cells from G1 to S phase and to stimulate DNA synthesis in pe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00012a002

    authors： Cao QP,McGrath CA,Baril EF,Quesenberry PJ,Reddy GP

    更新日期：1995-03-28 00:00:00

  • Structural and dynamic differences between normal and transforming N-ras gene products: a 31P and isotope-edited 1H NMR study.

    abstract：:[15N]Glycine was biosynthetically incorporated into normal cellular N-ras p21 and a position 12 transforming mutant, in order to produce p21 proteins containing several site-specific NMR probes at or near activating positions in the guanine nucleotide binding domain. We have previously assigned all five glycine resona...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00450a035

    authors： Campbell-Burk S

    更新日期：1989-11-28 00:00:00

  • Fidelity of HIV-1 reverse transcriptase copying RNA in vitro.

    abstract：:The genomic hypervariation of human immunodeficiency virus 1 (HIV-1) could result from misincorporations by the viral reverse transcriptase. We developed an assay for reverse transcriptase fidelity during RNA-dependent as well as DNA-dependent DNA polymerization in vitro. A lacZ alpha RNA fragment transcribed by T3 RN...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00119a002

    authors： Ji JP,Loeb LA

    更新日期：1992-02-04 00:00:00

  • Stopped-flow analysis on anion binding to blue-form halorhodopsin from Natronobacterium pharaonis: comparison with the anion-uptake process during the photocycle.

    abstract：:Pharaonis halorhodopsin (phR), the light-driven chloride ion pump from Natronobacterium pharaonis with C-terminal histidine tag, was expressed in Escherichia coli cells. The protein was solubilized with 0.1% n-dodecyl beta-D-maltopyranoside and purified with a nickel column. Removal of Cl- from the medium yields blue ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011788g

    authors： Sato M,Kanamori T,Kamo N,Demura M,Nitta K

    更新日期：2002-02-19 00:00:00