当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Identification of a new P450 expressed in human lung: complete cDNA sequence, cDNA-directed expression, and chromosome mapping.

Identification of a new P450 expressed in human lung: complete cDNA sequence, cDNA-directed expression, and chromosome mapping.

Abstract：

:A cDNA coding for a P450 expressed in human lung was isolated from a lambda gt11 library constructed from human lung mRNA using a cDNA probe to rat P450 IVA1. The cDNA-deduced amino acid sequence of this P450, designated IVB1, consisted of 511 amino acids and had a calculated molecular weight of 59,558. The IVB1 amino acid sequence bore 51%, 53%, and 52% similarities to rat IVA1, IVA2, and rabbit P450p-2, respectively. Comparison of the primary amino acid sequence of human IVB1 with rat IVA and rabbit p-2 P450 sequences revealed a region of absolute sequence identity of 17 amino acids between residues 304 and 320. However, the functional significance of this conserved sequence is unknown. Human IVB1 also appears to be related to P450 isozyme 5 that has been extensively characterized in rabbits. The IVB1 cDNA was inserted into a vaccinia virus expression vector and the enzyme expressed in human cell lines. The expressed enzyme had an absorption spectrum with a lambda max at 450 nm when reduced and complexed with carbon monoxide, typical of other cytochrome P450s. Unlike rabbit P450 isozyme 5, however, human IVB1 was unable to activate the promutagen 2-aminofluorene. Human lung microsomal P450s were also unable to metabolize this compound despite the presence of IVB1 mRNA in three out of four human lungs analyzed. In contrast to its expression in lung, IVB1 mRNA was undetectable in livers from 14 individuals, including those from which the lungs were derived. IVB1-related mRNA was also expressed in rat lung and was undetectable in untreated rat liver.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Nhamburo PT,Gonzalez FJ,McBride OW,Gelboin HV,Kimura S

doi

10.1021/bi00446a014

subject

Has Abstract

pub_date

1989-10-03 00:00:00

pages

8060-6

issue

20

eissn

0006-2960

issn

1520-4995

journal_volume

28

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Kinetic and modeling studies of S3-S3' subsites of HIV proteinases.

    abstract：:Kinetic analysis and modeling studies of HIV-1 and HIV-2 proteinases were carried out using the oligopeptide substrate [formula: see text] and its analogs containing single amino acid substitutions in P3-P3' positions. The two proteinases acted similarly on the substrates except those having certain hydrophobic amino ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00135a008

    authors： Tözsér J,Weber IT,Gustchina A,Bláha I,Copeland TD,Louis JM,Oroszlan S

    更新日期：1992-05-26 00:00:00

  • Glyoxylate carboligase: a unique thiamin diphosphate-dependent enzyme that can cycle between the 4'-aminopyrimidinium and 1',4'-iminopyrimidine tautomeric forms in the absence of the conserved glutamate.

    abstract：:Glyoxylate carboligase (GCL) is a thiamin diphosphate (ThDP)-dependent enzyme, which catalyzes the decarboxylation of glyoxylate and ligation to a second molecule of glyoxylate to form tartronate semialdehyde (TSA). This enzyme is unique among ThDP enzymes in that it lacks a conserved glutamate near the N1' atom of Th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300893v

    authors： Nemeria N,Binshtein E,Patel H,Balakrishnan A,Vered I,Shaanan B,Barak Z,Chipman D,Jordan F

    更新日期：2012-10-09 00:00:00

  • Xylose isomerase in substrate and inhibitor michaelis states: atomic resolution studies of a metal-mediated hydride shift.

    abstract：:Xylose isomerase (E.C. 5.3.1.5) catalyzes the interconversion of aldose and ketose sugars and has an absolute requirement for two divalent cations at its active site to drive the hydride transfer rates of sugar isomerization. Evidence suggests some degree of metal movement at the second metal site, although how this m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049812o

    authors： Fenn TD,Ringe D,Petsko GA

    更新日期：2004-06-01 00:00:00

  • Thermodynamic consequences of bipartite immunity protein binding to the ribosomal ribonuclease colicin E3.

    abstract：:Colicin E3 is a 60 kDa, multidomain protein antibiotic that targets its ribonuclease activity to an essential region of the 16S ribosomal RNA of Escherichia coli. To prevent suicide of the producing cell, synthesis of the toxin is accompanied by the production of a 10 kDa immunity protein (Im3) that binds strongly to ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0273720

    authors： Walker D,Moore GR,James R,Kleanthous C

    更新日期：2003-04-15 00:00:00

  • Translesional synthesis on a DNA template containing a single stereoisomer of dG-(+)- or dG-(-)-anti-BPDE (7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene).

    abstract：:Oligodeoxynucleotides modified site-specifically with dG-(+)-trans- and dG-(+)-cis-anti-BPDE (7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene) or dG-(-)-trans- and dG-(-)-cis-anti-BPDE were used as templates in primer extension reactions catalyzed by the Klenow fragment of Escherichia coli DNA polymerase I....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00080a027

    authors： Shibutani S,Margulis LA,Geacintov NE,Grollman AP

    更新日期：1993-07-27 00:00:00

  • Catalytic function and local proton structure at the type 2 copper of nitrite reductase: the correlation of enzymatic pH dependence, conserved residues, and proton hyperfine structure.

    abstract：:Electron nuclear double resonance (ENDOR) of protons at Type 2 and Type 1 cupric active sites correlates with the enzymatic pH dependence, the mutation of nearby conserved, nonligating residues, and electron transfer in heterologously expressed Rhodobacter sphaeroides nitrite reductase. Wild-type enzyme showed a pH 6 ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0256274

    authors： Zhao Y,Lukoyanov DA,Toropov YV,Wu K,Shapleigh JP,Scholes CP

    更新日期：2002-06-11 00:00:00

  • Rat kidney L-alpha-hydroxy acid oxidase: isolation of enzyme with one flavine coenzyme per two subunits.

    abstract：:L-alpha-Hydroxy acid oxidase (listed as EC 1.4.3.2, L-amino acid: O2 oxidoreductase) has been purified 100-fold from rat kidney to apparent homogeneity by gel electrophoresis. A subunit molecular weight of 47,500 was found by sodium dodecyl sulfate gel electrophoresis, but in contrast to previous reports, the enzyme h...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00683a005

    authors： Cromartie TH,Walsh CT

    更新日期：1975-06-17 00:00:00

  • Bidirectional Transformation of a Metamorphic Protein between the Water-Soluble and Transmembrane Native States.

    abstract：:The bidirectional transformation of a protein between its native water-soluble and integral transmembrane conformations is demonstrated for FraC, a hemolytic protein of the family of pore-forming toxins. In the presence of biological membranes, the water-soluble conformation of FraC undergoes a remarkable structural r...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b01112

    authors： Tanaka K,Caaveiro JM,Tsumoto K

    更新日期：2015-11-24 00:00:00

  • Structural Studies of AAV2 Rep68 Reveal a Partially Structured Linker and Compact Domain Conformation.

    abstract：:Adeno-associated virus (AAV) nonstructural proteins Rep78 and Rep68 carry out all DNA transactions that regulate the AAV life cycle. They share two multifunctional domains: an N-terminal origin binding/nicking domain (OBD) from the HUH superfamily and a SF3 helicase domain. A short linker of ∼20 amino acids that is cr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00610

    authors： Musayev FN,Zarate-Perez F,Bardelli M,Bishop C,Saniev EF,Linden RM,Henckaerts E,Escalante CR

    更新日期：2015-09-29 00:00:00

  • Interaction of divalent cations with beta-galactosidase (Escherichia coli).

    abstract：:Although the addition of various divalent metals to beta-galactosidase resulted in apparent activation, only Mg2+ and Mn2+ actually did activate. The apparent activation by the other divalent metals was shown to be due to Mg2+ impurities. Calcium did not activate, but experiments suggested that it did bind. Other diva...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00586a005

    authors： Huber RE,Parfett C,Woulfe-Flanagan H,Thompson DJ

    更新日期：1979-09-18 00:00:00

  • Determination of Fe-ligand bond lengths and the Fe-N-O bond angles in soybean ferrous and ferric nitrosylleghemoglobin a using multiple-scattering XAFS analyses.

    abstract：:The NO adducts of leghemoglobin (Lb) are implicated in biological processes, but only the adduct with ferrous Lb (Lb(II)NO) has been characterized previously. We report the first characterization of ferric nitrosylleghemoglobin (Lb(III)NO) and XAS experiments performed on frozen aqueous solutions of Lb(II)NO and Lb(II...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990730n

    authors： Rich AM,Ellis PJ,Tennant L,Wright PE,Armstrong RS,Lay PA

    更新日期：1999-12-14 00:00:00

  • Penicillanic acid sulfone: an unexpected isotope effect in the interaction of 6 alpha- and 6 beta-monodeuterio and of 6,6-dideuterio derivatives with RTEM beta-lactamase from Escherichia coli.

    abstract：:Penicillanic acid sulfone (1) is both a substrate and an inactivator of the RTEM beta-lactamase. About 7000 hydrolytic events occur before enzyme inactivation. The 6,6-dideuterio sulfone shows a 3-fold acceleration of both the hydrolysis reaction and the enzyme inactivation. The kinetic and spectroscopic results are n...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00516a003

    authors： Brenner DG,Knowles JR

    更新日期：1981-06-23 00:00:00

  • Structure of Alcaligenes faecalis nitrite reductase and a copper site mutant, M150E, that contains zinc.

    abstract：:The structures at 2.0 and 2.25 A resolution of native and recombinant nitrite reductase from Alcaligenes faecalis show that they are identical to each other and very similar to nitrite reductase from Achromobacter cycloclastes. The crystallographic structure of a mutant, M150E, which unlike the wild-type protein canno...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00038a003

    authors： Murphy ME,Turley S,Kukimoto M,Nishiyama M,Horinouchi S,Sasaki H,Tanokura M,Adman ET

    更新日期：1995-09-26 00:00:00

  • Electron/proton coupling in bacterial nitric oxide reductase during reduction of oxygen.

    abstract：:The respiratory nitric oxide reductase (NOR) from Paracoccus denitrificans catalyzes the two-electron reduction of NO to N(2)O (2NO + 2H(+) + 2e(-) --> N(2)O + H(2)O), which is an obligatory step in the sequential reduction of nitrate to dinitrogen known as denitrification. NOR has four redox-active cofactors, namely,...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050524h

    authors： Flock U,Watmough NJ,Adelroth P

    更新日期：2005-08-09 00:00:00

  • Aminoacyl thioester chemistry of class II aminoacyl-tRNA synthetases.

    abstract：:Lysyl-tRNA synthetase, a class II enzyme, edits homocysteine by converting it into homocysteine thiolactone. In a similar reaction, the enzyme converts homoserine into homoserine lactone. Other class II enzymes, aspartyl-tRNA synthetase and seryl-tRNA synthetase, do not edit any of the amino acids tested. However, all...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970589n

    authors： Jakubowski H

    更新日期：1997-09-16 00:00:00

  • Cooperative stabilization of transthyretin by clusterin and diflunisal.

    abstract：:The circulating protein transthyretin (TTR) can unfold, oligomerize, and form highly structured amyloid fibrils that are deposited in tissues, causing organ damage and disease. This pathogenic process is caused by a heritable TTR point mutation in cases of familial TTR-related amyloidosis or wild-type TTR in cases of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi5011249

    authors： Greene MJ,Klimtchuk ES,Seldin DC,Berk JL,Connors LH

    更新日期：2015-01-20 00:00:00

  • Sulphydryl group modification of aspartate aminotransferase with 3-bromo-1,1,1-trifluoropropanone during catalysis.

    abstract：:After protection of cysteine-45 and -82 with iodoacetamide or N-ethylmaleimide, and in the presence of saturating concentrations of substrates, the supernatant isozyme of pig heart aspartate transaminase has been covalently modified at cysteine-390 with 3-bromo-1,1,1-trifluoropropanone. The modified enzyme retains 60-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00627a004

    authors： Critz WJ,Martinez-Carrion M

    更新日期：1977-04-19 00:00:00

  • Effects of DNA binding proteins on DNA methylation in vitro.

    abstract：:The inheritance of DNA methylation patterns may play an important role in the stability of the differentiated state. We have therefore studied the inhibitory effects of DNA binding proteins on DNA methylation in vitro. Mouse L1210 cells grown in the presence of 5-azacytidine acquire hemimethylated sites in their DNA. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00326a021

    authors： Kautiainen TL,Jones PA

    更新日期：1985-02-26 00:00:00

  • Altering the reaction specificity of eukaryotic ornithine decarboxylase.

    abstract：:Ornithine decarboxylase (ODC) catalyzes the first committed step in the biosynthesis of polyamines, and it has been identified as a drug target for the treatment of African sleeping sickness, caused by Trypanosoma brucei. ODC is a pyridoxal 5'-phosphate (PLP) dependent enzyme and an obligate homodimer. X-ray structura...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001209s

    authors： Jackson LK,Brooks HB,Osterman AL,Goldsmith EJ,Phillips MA

    更新日期：2000-09-19 00:00:00

  • A new bifunctional spin-label suitable for saturation-transfer EPR studies of protein rotational motion.

    abstract：:A new bifunctional spin-label (BSL) has been synthesized that can be immobilized on the surface of proteins, allowing measurement of rotational motion of proteins by saturation-transfer electron paramagnetic resonance (STEPR). The spin-label contains a photoactivatable azido moiety, a cleavable disulfide, and a nitrox...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00476a014

    authors： Wilcox MD,Parce JW,Thomas MJ,Lyles DS

    更新日期：1990-06-19 00:00:00

  • Conserved phosphoprotein interaction motif is functionally interchangeable between ataxin-7 and arrestins.

    abstract：:Olivopontocerebellar atrophy with retinal degeneration is a hereditary neurodegenerative disorder that belongs to the subtype II of the autosomal dominant cerebellar ataxias and is characterized by early-onset cerebellar and macular degeneration preceded by diagnostically useful tritan colorblindness. The gene mutated...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992694y

    authors： Mushegian AR,Vishnivetskiy SA,Gurevich VV

    更新日期：2000-06-13 00:00:00

  • Crystal structures of the inactive D30N mutant of feline immunodeficiency virus protease complexed with a substrate and an inhibitor.

    abstract：:Crystal structures of complexes of a D30N mutant of feline immunodeficiency virus protease (FIV PR) complexed with a statine-based inhibitor (LP-149), as well as with a substrate based on a modification of this inhibitor (LP-149S), have been solved and refined at resolutions of 2.0 and 1.85 A, respectively. Both the i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9707436

    authors： Laco GS,Schalk-Hihi C,Lubkowski J,Morris G,Zdanov A,Olson A,Elder JH,Wlodawer A,Gustchina A

    更新日期：1997-09-02 00:00:00

  • Redesign of the proton-pumping machinery of cytochrome c oxidase: proton pumping does not require Glu(I-286).

    abstract：:One of the putative proton-transfer pathways leading from solution toward the binuclear center in many cytochrome c oxidases is the D-pathway, so-called because it starts with a highly conserved aspartate [D(I-132)] residue. Another highly conserved amino acid residue in this pathway, glutamate(I-286), has been indica...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0012641

    authors： Aagaard A,Gilderson G,Mills DA,Ferguson-Miller S,Brzezinski P

    更新日期：2000-12-26 00:00:00

  • Biochemical characterization of the tetrodotoxin binding protein from Electrophorus electricus.

    abstract：:Biochemical properties of a detergent-solubilized tetrodotoxin binding component from Electrophorus electricus have been examined and compared with those found for the membrane-bound protein. The toxin binding component was solubilized with high efficiency by a variety of nonionic detergents and with lower efficiency ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00267a029

    authors： Moore AC,Agnew WS,Raftery MA

    更新日期：1982-11-23 00:00:00

  • Stimulation of RecA-mediated D-loop formation by oligonucleotide-directed triple-helix formation: guided homologous recombination (GOREC).

    abstract：:Oligonucleotide-directed triple helix formation provides an elegant rational basis for gene-specific DNA targeting and has been widely used to interfere with gene expression ("antigene" strategies) and as a molecular tool for biological studies. Various strategies have been developed to introduce sequence modification...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001605a

    authors： Biet E,Maurisse R,Dutreix M,Sun Js

    更新日期：2001-02-13 00:00:00

  • Antiapoptotic signaling by the insulin receptor in Chinese hamster ovary cells.

    abstract：:We have sought to determine whether insulin can promote cell survival and protect Chinese hamster ovary (CHO) cells from apoptosis induced by serum starvation. Low concentrations of insulin were antiapoptotic for cells overexpressing wild-type insulin receptors but not in cells transfected with kinase-defective insuli...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9805947

    authors： Lee-Kwon W,Park D,Baskar PV,Kole S,Bernier M

    更新日期：1998-11-10 00:00:00

  • Mutagenesis of the uncoupling protein of brown adipose tissue. Neutralization Of E190 largely abolishes pH control of nucleotide binding.

    abstract：:For expression in Saccharomyces cerevisiae the cDNA of the uncoupling protein (UCP) of brown adipose tissue from hamster has been isolated and used to transform yeast cells. Optimized expression conditions yielded 2% of mitochondrial protein as UCP. UCP was isolated, avoiding copurification of ADP/ATP carrier and pori...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970513r

    authors： Echtay KS,Bienengraeber M,Klingenberg M

    更新日期：1997-07-08 00:00:00

  • Enzymatic activation of DNA cleavage by dynemicin A and synthetic analogs.

    abstract：:Dynemicin A (1), a member of the enediyne family of natural products, binds to double-stranded DNA (K(B) approximately 10(4) M(-1)) and in the presence of millimolar concentrations of a reducing cofactor such as NADPH or GSH reacts to cleave DNA. In this work, we show that the two flavin-based enzymes ferredoxin-NADP+...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962976n

    authors： Myers AG,Kort ME,Cohen SB,Tom NJ

    更新日期：1997-04-01 00:00:00

  • Interaction of lipoprotein lipase with phospholipid vesicles: effect on protein and lipid structure.

    abstract：:The interaction of lipoprotein lipase (LpL) and a nonhydrolyzable phosphatidylcholine, 1,2-ditetradecyl-rac-glycero-3-phosphocholine (C14-ether-PC), has been studied by several physical methods. Analysis of the circular dichroic spectrum of LpL gave the following fractional conformation: 35% alpha-helix, 30% beta-plea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00352a020

    authors： McLean LR,Larsen WJ,Jackson RL

    更新日期：1986-02-25 00:00:00

  • Cytochrome c peroxidase mutant active site structures probed by resonance Raman and infrared signatures of the CO adducts.

    abstract：:Vibrational frequencies associated with FeC and CO stretching and FeCO bending modes have been determined via resonance Raman (RR) and infrared (IR) spectroscopy for cytochrome c peroxidase (CCP) mutants prepared by site-directed mutagenesis. These include the bacterial "wild type", CCP(MI), and mutations involving gr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00415a015

    authors： Smulevich G,Mauro JM,Fishel LA,English AM,Kraut J,Spiro TG

    更新日期：1988-07-26 00:00:00