Abstract:
:Electrogenic events associated with the activity of the melibiose permease (MelB), a transporter from Escherichia coli, were investigated. Proteoliposomes containing purified MelB were adsorbed to a solid supported lipid membrane, activated by a substrate concentration jump, and transient currents were measured. When the transporter was preincubated with Na(+) at saturating concentrations, a charge translocation in the protein upon melibiose binding could still be observed. This result demonstrates that binding of the uncharged substrate melibiose triggers a charge displacement in the protein. Further analysis showed that the charge displacement is neither related to extra Na(+) binding to the transporter, nor to the displacement of already bound Na(+) within the transporter. The electrogenic melibiose binding process is explained by a conformational change with concomitant displacement of charged amino acid side chains and/or a reorientation of helix dipoles. A kinetic model is suggested, in which Na(+) and melibiose binding are distinct electrogenic processes associated with approximately the same charge displacement. These binding reactions are fast in the presence of the respective cosubstrate (k > 50 s(-1)).
journal_name
Biochemistryjournal_title
Biochemistryauthors
Meyer-Lipp K,Ganea C,Pourcher T,Leblanc G,Fendler Kdoi
10.1021/bi0489053subject
Has Abstractpub_date
2004-10-05 00:00:00pages
12606-13issue
39eissn
0006-2960issn
1520-4995journal_volume
43pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Purified beta subunit of human chorionic gonadotropin (hCG) was partially reduced with beta-mercaptoethanol, carboxymethylated, and digested with chymotrypsin. The peptides were isolated by high-voltage electrophoresis and paper chromatography. Five major disulfide-containing peptides were isolated, and their location...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00619a034
更新日期:1978-12-26 00:00:00
abstract::Halorhodopsin from Natronomonas pharaonis (NpHR) acts an inward-directed, light-driven chloride pump and forms a homotrimer. To evaluate effect of trimeric assembly, that is, intermolecular interaction, on the control or modulation of light-driven chloride pumping activity of individual HRs, it is important to underst...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901380c
更新日期:2009-12-29 00:00:00
abstract::The mechanism of activation of cGMP phosphodiesterase by the GTP-binding protein in the disc membrane of retinal rods has been investigated by measuring the light-induced phosphodiesterase activity in reconstituted systems where the concentration of either the GTP-binding protein or the phosphodiesterase is varied. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00444a040
更新日期:1989-09-05 00:00:00
abstract::The bile acid cholate disrupts platelet plasma membrane vesicles and inactivates 5-hydroxytryptamine transport activity. The transporter appears not to be solubilized by this procedure, but remains associated with a high molecular weight aggregate. Merely removing cholate does not restore the vesicular structure or tr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00617a033
更新日期:1978-11-28 00:00:00
abstract::Synaptotagmin 1 (syt1) is a synaptic vesicle-anchored membrane protein that acts as the calcium sensor for the synchronous component of neuronal exocytosis. Using site-directed spin labeling, the position and membrane interactions of a fragment of syt1 containing its two C2 domains (syt1C2AB) were assessed in bilayers...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200049c
更新日期:2011-04-05 00:00:00
abstract::Doublet signal and singlet-like signal induced in Ca(2+)-depleted PS II were investigated by pulsed EPR in one-dimensionally oriented photosystem (PS) II membranes. The doublet signal showed marked angular dependent change in its spectrum in term of the applied magnetic field, indicating that the magnetic dipole-dipol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9929406
更新日期:2000-09-12 00:00:00
abstract::Using our hemoglobin expression system in Escherichia coli, we have constructed three recombinant hemoglobins (rHbs) with amino acid substitutions located in the alpha(1)beta(1) and alpha(1)beta(2) subunit interfaces and in the distal heme pocket of the alpha-chain: rHb(alphaV96W, betaN108K), rHb(alphaL29F, alphaV96W,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991271t
更新日期:1999-10-05 00:00:00
abstract::Calcium is required for the substrate binding and for the chemical step of the interfacial catalytic turnover cycle of pancreatic phospholipase A2 (PLA2), but not for the binding of the enzyme to the interface. The role of calcium and other divalent cations (C) is analyzed for the effect on the substrate binding and k...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9728607
更新日期:1998-09-08 00:00:00
abstract::Creatininase catalyzes the conversion of creatinine (a biosensor for kidney function) to creatine via a two-step mechanism: water addition followed by ring opening. Water addition is common to other known cyclic amidohydrolases, but the precise mechanism for ring opening is still under debate. The proton donor in this...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01032
更新日期:2017-12-05 00:00:00
abstract::The mechanism of bovine intestinal 5'-nucleotide phosphodiesterase was investigated by determining kinetic constants of systematically varied substrates, with emphasis on esters of phosphonic acids (which have much higer Vmax values than conventional phosphodiester substrates), and by pre-steady-state kinetics using b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00625a011
更新日期:1977-03-22 00:00:00
abstract::Phosphorus-31 magic angle sample spinning NMR spectra have been employed to investigate the structure and composition of the mineral deposits in chicken bone. Three different pulse sequences, Bloch decay, cross-polarization, and dipolar suppression, were employed to obtain spectra from bone specimens of varying age. T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00320a033
更新日期:1984-12-04 00:00:00
abstract::We have investigated, both experimentally and theoretically, the efflux of carboxyfluorescein (a self-quenching fluorescent dye) from vesicles of different sizes and lipid species (POPC, DOPC) after having added the bee venom peptide melittin. This comprises quantitative analyses regarding the extent of lipid-associat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971009p
更新日期:1998-02-24 00:00:00
abstract::RNA helicase DHX33 has been shown to participate in a variety of cellular activities, including ribosome biogenesis, protein translation, and gene transcription. We and others further discovered that DHX33 is strongly expressed in several types of human cancers and plays important roles in promoting cancer cell prolif...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00166
更新日期:2019-01-29 00:00:00
abstract::Photochemical and subsequent thermal reactions of rhodopsin containing 9-cis-retinal [Rh(9)] or one of four analogues with 9-cis geometries formed from ring-modified retinals, alpha-retinal [alpha Rh(9)], acyclic retinal [AcRh(9)], acyclic alpha-retinal [Ac alpha Rh(9)], and 5-isopropyl-alpha-retinal [P alpha Rh(9)] w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00233a022
更新日期:1991-05-14 00:00:00
abstract::Understanding the evolution of allostery in multidomain enzymes remains an important step in improving our ability to identify and exploit structure-function relationships in allosteric mechanisms. A recent protein similarity network for the DRE-TIM metallolyase superfamily indicated there are two evolutionarily disti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500702u
更新日期:2014-07-29 00:00:00
abstract::A simple and rapid procedure is described for purification of P-glycoprotein (Pgp) from a multidrug-resistant Chinese hamster ovary cell line (CR1R12) in which the plasma membranes are highly enriched in Pgp (Al-Shawi, M.K., Senior A.E. (1993) J. Biol. Chem, 268, 4197-4206). The procedure consisted of octylglucoside s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00189a008
更新日期:1994-06-14 00:00:00
abstract::The effects of mutation of the conserved serine92 residue to alanine, valine, and leucine in pig myoglobin have been determined. In myoglobin crystal structures, the hydroxyl group of serine92 is within hydrogen-bonding distance of the N delta-H of histidine93, whose N epsilon coordinates the iron atom of the heme pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00070a023
更新日期:1993-05-18 00:00:00
abstract::The flavoprotein nitroalkane oxidase catalyzes the oxidation of primary and secondary nitroalkanes to aldehydes and ketones, respectively, transferring electrons to oxygen to form hydrogen peroxide. The steady-state kinetic mechanism of the active flavin adenine dinucleotide-(FAD-) containing form of the enzyme has be...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9922547
更新日期:2000-02-15 00:00:00
abstract::The leech carboxypeptidase inhibitor (LCI) is a 66-amino acid protein, containing four disulfides that stabilize its structure. This polypeptide represents an excellent model for the study and understanding of the diversity of folding pathways in small, cysteine-rich proteins. The pathway of oxidative folding of LCI h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034308p
更新日期:2003-06-10 00:00:00
abstract::We report here that polyamide nucleic acid (PNA) as well as a polyamide nucleic acid-DNA chimera complementary to the primer binding site of the HIV-1 genome can completely block priming by tRNA3Lys and consequently the in vitro initiation of reverse transcription by HIV-1 RT. Conventional heating and cooling is not r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972197m
更新日期:1998-01-20 00:00:00
abstract::Human uracil DNA glycosylase (hUNG) plays a central role in DNA repair and programmed mutagenesis of Ig genes, requiring it to act on sparsely or densely spaced uracil bases located in a variety of contexts, including U/A and U/G base pairs, and potentially uracils within single-stranded DNA (ssDNA). An interesting qu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301562n
更新日期:2013-04-16 00:00:00
abstract::The covalent affinity probe maltosyl isothiocyanate (MITC) has been used previously to identify the glucose transporter of human erythrocytes as a component of band 3. By use of limited proteolysis, the site on the Mr 100 000 protein to which MITC attaches has been localized to a 17 000-dalton region near the center o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00331a001
更新日期:1985-05-07 00:00:00
abstract::A cutinase-type polyesterase from Saccharomonospora viridis AHK190 (Cut190) has been shown to degrade the inner block of polyethylene terephthalate. A unique feature of Cut190 is that its function and stability are regulated by Ca2+ binding. Our previous crystal structure analysis of Cut190S226P showed that one Ca2+ b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00624
更新日期:2018-09-11 00:00:00
abstract::We have studied the physical and functional linkages of heterologously expressed human 5-HT1A receptors to G protein alpha-subunits in HeLa and CHO-K1 cells. HeLa cells expressed immunoreactivity to G(i) proteins with an apparent rank order of G(i) alpha 3 (approximately 1 pmol/mg of protein) > G(i) alpha 1 (approxima...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00092a016
更新日期:1993-10-19 00:00:00
abstract::Modified (Arg63-Ile64 reactive-site peptide bond hydrolyzed) soybean trypsin inhibitor (Kunitz) with all reactive amino groups, except that of Ile64, protected was described in the preceding paper (Kowalski, D., and Laskowski, M., Jr. (1976), Biochemistry, preceding paper in this issue). Treatment of this inhibitor wi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00651a022
更新日期:1976-03-23 00:00:00
abstract::The formation and role of arachidonic acid (AA) and its metabolites during gonadotropin releasing hormone- (GnRH-) induced gonadotropin secretion were investigated in primary cultures of rat pituitary cells. Prelabeled cells ([3H]AA) responded to GnRH challenge with increased formation (about 2-fold) of the leukotrien...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00139a004
更新日期:1992-06-23 00:00:00
abstract::RS1, also known as retinoschisin, is an extracellular discoidin domain-containing protein that has been implicated in maintaining the cellular organization and synaptic structure of the vertebrate retina. Mutations in the gene encoding RS1 are responsible for X-linked retinoschisis, a retinal degenerative disease char...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800938g
更新日期:2008-09-02 00:00:00
abstract::The mechanism by which newly synthesized sterols are transported from their site of synthesis, the endoplasmic reticulum (ER), to the sterol-enriched plasma membrane (PM) is not fully understood. Studies in mammalian cells suggest that newly synthesized cholesterol is transported to the PM in Golgi-bypassing vesicles ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048296z
更新日期:2005-04-19 00:00:00
abstract::The S100 proteins comprise a group of EF-hand proteins that undergo a calcium-induced conformational change allowing them to interact with other proteins and produce a biological response. A unique feature of these proteins is the fact that they can form both homo- and heterodimers independent of calcium binding. The ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi802086a
更新日期:2009-03-10 00:00:00
abstract::Tau fibrillization is a potential therapeutic target for Alzheimer's and other neurodegenerative diseases. Small molecules capable of both inhibiting aggregation and promoting filament disaggregation have been discovered, but knowledge of their mechanism of action and potential for testing in biological models is frag...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050387o
更新日期:2005-08-02 00:00:00