Abstract:
:The bile acid cholate disrupts platelet plasma membrane vesicles and inactivates 5-hydroxytryptamine transport activity. The transporter appears not to be solubilized by this procedure, but remains associated with a high molecular weight aggregate. Merely removing cholate does not restore the vesicular structure or transport activity, but in the presence of added soybean phospholipids, cholate removal generates proteoliposomes which accumulate 5-hydroxytryptamine. This reconstituted transport activity is similar in its ionic requirements and inhibitor sensitivity to transport in native membrane vesicles and intact platelets.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Rudnick G,Nelson PJdoi
10.1021/bi00617a033subject
Has Abstractpub_date
1978-11-28 00:00:00pages
5300-3issue
24eissn
0006-2960issn
1520-4995journal_volume
17pub_type
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