Abstract：
:The diiron active sites of the purple acid phosphatases from porcine uterus (also called uteroferrin, Uf) and bovine spleen (BSPAP) and their complexes with tungstate are compared by 1H NMR and NOE techniques. The paramagnetically shifted features of the 1H NMR spectrum of reduced BSPAP are similar to those of reduced Uf, while the spectra of the tungstate complexes are almost identical. These observations suggest that the two active sites are quite similar, in agreement with the greater than 90% sequence homology found in the two enzymes. Nuclear Overhauser effect (NOE) experiments on the His N-H resonances show that the Fe(III)-His residue is N epsilon-coordinated, while the Fe(II)-His is H delta-coordinated in both enzymes. On the basis of the above NMR and NOE results, our previously proposed model for the dinuclear iron active site of Uf [Scarrow, R. C., Pyrz, J. W., & Que, L., Jr. (1990) J. Am. Chem. Soc. 112, 657-665] is corroborated, refined, and found to represent the diiron center of BSPAP as well.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Wang Z,Ming LJ,Que L Jr,Vincent JB,Crowder MW,Averill BAdoi
10.1021/bi00138a004subject
Has Abstractpub_date
1992-06-16 00:00:00pages
5263-8issue
23eissn
0006-2960issn
1520-4995journal_volume
31pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The PB1 domain of NBR1 folds via a single pathway mechanism involving two sequential energy barriers separated by a high-energy intermediate. The structural ensemble representing each of the two transition states (TS1 and TS2) has been calculated using experimental Φ values and biased molecular dynamics simulations. B...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1016793
更新日期：2011-01-11 00:00:00
abstract：:alpha-Glucosidase (EC 3.2.1.20) was purified to homogeneity from logarithmically growing cells of Saccharomyces carlsbergensis. The purification involved the following steps: (a) ammonium sulfate fractionation; (b) Sephadex G-100 chromatography; (c) DEAE-cellulose chromatography; and (d) hydroxylapatite chromatography...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00615a011
更新日期：1978-10-31 00:00:00
abstract：:The metalloregulatory protein MntR from Bacillus subtilis acts as a transcriptional regulator of manganese homeostasis. MntR is a member of a subfamily of DtxR-related proteins that perform analogous regulatory functions in a variety of pathogenic organisms. Metal ions activate MntR to bind DNA and repress the transcr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0350248
更新日期：2003-11-04 00:00:00
abstract：:Casein kinase II is a widely distributed protein serine/threonine kinase. The holoenzyme appears to be a tetramer, containing two alpha or alpha' subunits (or one of each) and two beta subunits. Complementary DNA clones encoding the subunits of casein kinase II were isolated from a human T-cell lambda gt10 library usi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00488a034
更新日期：1990-09-11 00:00:00
abstract：:Many serine hydrolases catalyze perhydrolysis, the reversible formation of peracids from carboxylic acids and hydrogen peroxide. Recently, we showed that a single amino acid substitution in the alcohol binding pocket, L29P, in Pseudomonas fluorescens (SIK WI) aryl esterase (PFE) increased the specificity constant of P...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9021268
更新日期：2010-03-09 00:00:00
abstract：:The left-handed polyproline II (P(II)) helix is a structure that has been given a great deal of attention lately because of its role in a wide variety of physiologically important processes and potential significance in protein unfolded states. Recent work by several authors has shown that residues besides proline can...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049922v
更新日期：2004-05-18 00:00:00
abstract：:The synthesis of activated sulfate (adenosine 5'-phosphosulfate, APS) and inorganic pyrophosphate from ATP and SO4 is remarkably unfavorable: K(eq) approximately 10(-8) under presumed, near-physiological conditions. Consequently, ATP sulfurylases, which catalyze APS synthesis, suffer approximately 10(8)-fold losses in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060421e
更新日期：2006-09-26 00:00:00
abstract：:An X-ray structure analysis of a crystal of mutant Asp229----Ala of cyclodextrin glycosyltransferase from Bacillus circulans (Ec 2.4.1.19) that had been shortly exposed to beta-cyclodextrin showed density corresponding to a maltose bound at the catalytic center. The crystal structure was refined to an R-factor of 18.7...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00152a009
更新日期：1992-09-22 00:00:00
abstract：:The structural and functional properties of G protein-coupled receptors (GPCRs) are often studied in a detergent micellar environment, but many GPCRs tend to denature or aggregate in short alkyl chain detergents. In our previous work [Lee, S., et al. (2016) J. Am. Chem. Soc.138, 15425-15433], we showed that GPCRs in a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00183
更新日期：2020-06-16 00:00:00
abstract：:We have investigated the formation of the aa-tRNA X EF-Tu X GTP ternary complex spectroscopically by monitoring a fluorescence change that accompanies the association of EF-Tu X GTP with Phe-tRNAPhe-F8, a functionally active analogue of Phe-tRNAPhe with a fluorescein moiety covalently attached to the s4U-8 base. With ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00324a023
更新日期：1985-01-29 00:00:00
abstract：:The conformation of the GM3 ganglioside, Neu5Ac alpha 2-3Gal beta 1-4Glc beta 1-1 Cer, and its analogs containing the Neu5Gc or Neu4Ac5Gc residues (Gc = glycolyl, CH2OHCO) was investigated in Me2SO-d6 solution with the aid of a distance-mapping procedure based on rotating-frame NOE contacts, with hydroxyl protons bein...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00145a014
更新日期：1992-08-04 00:00:00
abstract：:Measurements of the magnetic field dependence of the longitudinal magnetic relaxation rates (NMRD profiles) of solvent protons and deuterons led to the discovery of two classes of solvent binding sites in Ca2+-Mn2+-concanavalin A (CMPL) [Koenig, S. H., Brown, R. D., III, & Brewer, C. F. (1985) Biochemistry (second of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00340a004
更新日期：1985-09-10 00:00:00
abstract：:Apolipoprotein E is immunochemically localized to amyloid plaque in Alzheimer's brains, and the allelic distribution of ApoE in individuals is associated with a disposition toward Alzheimer's disease. We show here that all three ApoE isotypes exhibit a strong and specific ability to inhibit both nucleation and seeding...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961074j
更新日期：1996-09-24 00:00:00
abstract：:Wideline 2H NMR spectroscopy was used to assess the conformational and orientational effects of N-acetylneuraminic acid (NeuAc) (sialic acid) as a component of a particular oligosaccharide chain at a bilayer membrane surface. For this purpose, three glycosphingolipids, sharing a neutral core tetrasaccharide and differ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952964m
更新日期：1996-04-16 00:00:00
abstract：:The structure and folding of a novel human insulin mutant, [Thr(B27) --> Pro, Pro(B28) --> Thr]insulin (PT insulin), in aqueous solution and in mixtures of water and 2,2,2-trifluoroethanol (TFE) have been studied by NMR spectroscopy. It was found that PT insulin has a highly flexible structure in pure water and is pre...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0108150
更新日期：2001-09-04 00:00:00
abstract：:The dissociation of the trypsin-sensitive basic tails of the core histones in core chromatin has been followed as a function of [NaCl] using proton NMR spectroscopy. The tails dissociate in a highly cooperative all or none manner over the salt concentration range 0.2-0.6 M, that is, below the salt concentration requir...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00318a037
更新日期：1984-11-06 00:00:00
abstract：:We have synthesized Deltalac-acetogenins that are new acetogenin mimics possessing two n-alkyl tails without an alpha,beta-unsaturated gamma-lactone ring and suggested that their inhibition mechanism may be different from that of common acetogenins [Hamada et al. (2004) Biochemistry 43, 3651-3658]. To elucidate the in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0479003
更新日期：2005-01-18 00:00:00
abstract：:A common motif in protein structures is the assembly of alpha-helices. Natural alpha-helical assemblies, such as helical bundles and coiled coils, consist of multiple right-handed alpha-helices. Here we design a protein complex containing both left-handed and right-handed helices, with peptides of D- and L-amino acids...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010725v
更新日期：2001-07-31 00:00:00
abstract：:A manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from Arthrobacter globiformis strain CM-2 (MndD) cloned in Escherichia coli has been purified to homogeneity. Sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 Da). Steady-state kinetic analysis of Mnd...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi951979h
更新日期：1996-01-09 00:00:00
abstract：:Surface plasmon resonance (SPR) spectroscopy has been used to follow incorporation and light-induced conformational changes in bovine rhodopsin reconstituted into an egg phosphatidylcholine bilayer deposited on a thin silver film. The magnitude of the SPR spectral changes caused by light varies with pH in a manner par...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00250a022
更新日期：1994-11-22 00:00:00
abstract：:Intrinsically disordered proteins (IDPs) are functionally active despite lacking a well-defined three-dimensional structure. Such proteins often undergo a disorder-to-order transition, or induced folding, when binding to their specific physiological partner. Because of cooperativity, the folding and binding steps typi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00350
更新日期：2017-07-25 00:00:00
abstract：:Catalysis in protein tyrosine phosphatases (PTPs) involves movement of a protein loop called the WPD loop that brings a conserved aspartic acid into the active site to function as a general acid. Mutation of the tryptophan in the WPD loop of the PTP YopH to any other residue with a planar, aromatic side chain (phenyla...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00496
更新日期：2015-10-27 00:00:00
abstract：:Resonance Raman spectra of squid rhodopsin have been obtained under a variety of temperature and illumination conditions. The data have been characterized in terms of spectral contributions from squid rhodopsin, isorhodopsin, bathorhodopsin, lumirhodopsin, mesorhodopsin, P-465, and acid metarhodopsin. The results are ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00615a018
更新日期：1978-10-31 00:00:00
abstract：:Large conductance Ca(2+)-activated K(+) channels (BK(Ca)) contain an intracellular binding site for bovine pancreatic trypsin inhibitor (BPTI), a well-known inhibitor of various serine proteinase (SerP) enzymes. To investigate the structural basis of this interaction, we examined the activity of 11 BPTI mutants using ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992140v
更新日期：2000-02-29 00:00:00
abstract：:RXPA380 (Cbz-PhePsi[PO(2)CH]Pro-Trp-OH) was reported recently as the first highly selective inhibitor of the C-domain of somatic angiotensin-converting enzyme (ACE), able to differentiate the two active sites of somatic ACE by a selectivity factor of more than 3 orders of magnitude. The contribution of each RXPA380 re...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049504q
更新日期：2004-06-29 00:00:00
abstract：:RecA-catalyzed DNA recombination is initiated by a mandatory, high-energy form of DNA in RecA-nucleoprotein filaments, where bases are highly unstacked and the backbone is highly unwound. Interestingly, only the energetics consequent to adenosine triphosphate (ATP) binding, rather than its hydrolysis, seems sufficient...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034667k
更新日期：2003-10-21 00:00:00
abstract：:The retaining beta-1,4-glycanase Cex from Cellulomonas fimi, a family 10 glycosyl hydrolase, hydrolyzes xylan 40-fold more efficiently than cellulose. To gain insight into the nature of its preference for xylan, we determined the crystal structure of the Cex catalytic domain (Cex-cd) trapped as its covalent 2-deoxy-2-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9729211
更新日期：1998-04-07 00:00:00
abstract：:Human type-alpha transforming growth factor (hTGF alpha) is a small mitogenic protein containing 50 amino acids and three disulfide bonds. It has both sequence and structural homology with epidermal growth factor (EGF). While the three-dimensional structures of hTGF alpha and other EGF-like proteins have been studied ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00008a003
更新日期：1995-02-28 00:00:00
abstract：:Reconstitution of highly purified aquaporin CHIP (channel-forming integral protein) into proteoliposomes was previously shown to confer high osmotic water permeability (Pf) to the membranes [Zeidel et al. (1992) Biochemistry 31, 7436-7440]. Here we report detailed ultrastructural, pharmacologic, and transport studies ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00172a042
更新日期：1994-02-15 00:00:00
abstract：:The effects of several divalent cations, various polybasic amines, and lysozyme on the rate of polymerization of 8S clathrin to the 300S coat structure have been evaluated by turbidimetric procedures. Ca2+ and Mn2+ strongly enhance the rate of polymerization. Only spermine among the naturally occurring polybasic amine...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00526a028
更新日期：1981-11-10 00:00:00
