Site-directed mutagenesis of a predicted cation binding site of Na, K-ATPase.

abstract：:Ornithine decarboxylase (ODC) catalyzes the first committed step in the biosynthesis of polyamines, and it has been identified as a drug target for the treatment of African sleeping sickness, caused by Trypanosoma brucei. ODC is a pyridoxal 5'-phosphate (PLP) dependent enzyme and an obligate homodimer. X-ray structura...

journal_title：Biochemistry

authors： Jackson LK,Brooks HB,Osterman AL,Goldsmith EJ,Phillips MA

更新日期：2000-09-19 00:00:00

abstract：:Phenylcarbene and adamantylidene have been generated photochemically from the corresponding diazirines within lipid bilayers. Reasonable yields of labeled fatty acid side chains have been observed. The products have been characterized by gas chromatography-mass spectrometry and derive both from the insertion of the c...

journal_title：Biochemistry

authors： Bayley H,Knowles JR

更新日期：1978-06-13 00:00:00

abstract：:Escherichia coli cAMP receptor protein (CRP) controls more than 20 genes. There are significant differences in the promoter regions in these genes. Thus, an elucidation of the mechanism of CRP action requires knowledge about the mode of selectivity in these promoters. An earlier study [Heyduk, T., & Lee, J. C. (1990) ...

journal_title：Biochemistry

authors： Pyles EA,Lee JC

更新日期：1996-01-30 00:00:00

abstract：:The ability of several DNA polymerases to catalyze the template-directed synthesis of duplex oligonucleotides containing a base pair between a nucleotide with anhydrohexitol ring and its natural complement has been investigated. All DNA polymerases were able to accept the chemically synthesized anhydrohexitol triphosp...

journal_title：Biochemistry

authors： Vastmans K,Pochet S,Peys A,Kerremans L,Van Aerschot A,Hendrix C,Marlière P,Herdewijn P

更新日期：2000-10-24 00:00:00

abstract：:We report a straightforward enzymatic synthesis of the 4-methylumbelliferyl glycoside of a complex-type oligosaccharide substrate for core fucosylation. We demonstrate the use of this synthetic glycoconjugate in a newly developed enzyme assay to probe the activity and inhibition of fucosyltransferase VIII, which catal...

journal_title：Biochemistry

authors： Soroko M,Kwan DH

更新日期：2020-06-09 00:00:00

abstract：:Aquifex aeolicus 3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS) is active with a variety of different divalent metal ions bound in the active site. The Cd(2+), Zn(2+), and Cu(2+) substituted enzymes display similar values of k(cat) and similar dependence of K(m)(PEP) and K(m)(A5P) on both substrate and pro...

journal_title：Biochemistry

authors： Kona F,Tao P,Martin P,Xu X,Gatti DL

更新日期：2009-04-28 00:00:00

abstract：:The effect of calcium ion on the thermal stability of thermolysin has been investigated. The native protein undergoes an irreversible structural change and autolysis at high temperature. Analysis of the calcium ion dependence of the apparent melting temperature observed spectroscopically gives an apparent deltaH of -x...

journal_title：Biochemistry

authors： Dahlquist FW,Long JW,Bigbee WL

更新日期：1976-03-09 00:00:00

abstract：:The functional role of the Ca (2+) ion in the oxygen-evolving complex of photosystem II is not yet clear. Current models explain why the redox cycle of the complex would be interrupted after the S 3 state without Ca (2+), but the literature shows that it is interrupted after the S 2 state. Reinterpretation of the lite...

journal_title：Biochemistry

authors： Miqyass M,Marosvölgyi MA,Nagel Z,Yocum CF,van Gorkom HJ

更新日期：2008-07-29 00:00:00

abstract：:Myc and Max belong to the b-HLH-LZ family of transcription factors. Heterodimerization between Myc and Max or homodimerization of Max allows these proteins to bind their cognate DNA sequence known as the E-box (CACGTG). Recent evidence has suggested that the c-Myc/Max heterodimeric b-HLH-LZ could interact to form a he...

journal_title：Biochemistry

authors： Lebel R,McDuff FO,Lavigne P,Grandbois M

更新日期：2007-09-11 00:00:00

abstract：:Integrin conformational changes mediate integrin activation and signaling triggered by intracellular molecules or extracellular ligands. Even though it is known that αβ transmembrane domain separation is required for integrin signaling, it is still not clear how this signal is transmitted from the transmembrane domain...

journal_title：Biochemistry

authors： Wang W,Fu G,Luo BH

更新日期：2010-11-30 00:00:00

abstract：:Aromatic amine dehydrogenase (AADH) is the second enzyme known to possess the tryptophan tryptophylquinone (TTQ) prosthetic group. Its ability to catalyze the oxidative deamination of a wide range of aromatic and aliphatic amines has been investigated. Steady-state and transient kinetic studies of the reaction of AADH...

journal_title：Biochemistry

authors： Hyun YL,Davidson VL

更新日期：1995-01-24 00:00:00

abstract：:The existence of two conformational states of concanavalin A (Con A) with different metal ion binding properties has been recently demonstrated (Brown, R. D., Brewer, C. F., & Koenig, S. H. (1977) Biochemistry 16, 3883). Introduction of Mn2+ to the S1 site and Ca2+ to the S2 site of apo-Con A was shown to induce a con...

journal_title：Biochemistry

authors： Koenig SH,Brewer CF,Brown RD 3rd

更新日期：1978-10-03 00:00:00

abstract：:Scaffold/matrix-attached regions (S/MARs) are cis-acting elements with a function outside transcribed regions and in introns. Although they usually augment transcriptional rates, their action is highly context-dependent. We cloned an 800 bp S/MAR element from the upstream border of the human interferon-beta domain at ...

journal_title：Biochemistry

authors： Schübeler D,Mielke C,Maass K,Bode J

更新日期：1996-08-27 00:00:00

abstract：:A major regulatory step in the heat-induced transcription of heat shock protein (hsp) genes in eukaryotes is the activation of heat shock factor (HSF). In metazoans and Schizosaccharomyces pombe, HSF is present in unstressed cells but is unable to bind to its target DNA sequence element, the heat shock element (HSE). ...

journal_title：Biochemistry

authors： Larson JS,Schuetz TJ,Kingston RE

更新日期：1995-02-14 00:00:00

abstract：:The major covalently linked multimolecular D fragments found in plasmic digests of factor XIIIa cross-linked fibrin formed under physiological pH and ionic strength conditions consist of D dimers, D trimers, and D tetramers. These fragments are linked by epsilon-amino-gamma-glutamyllysine bonds in the carboxy-terminal...

journal_title：Biochemistry

authors： Siebenlist KR,Mosesson MW

更新日期：1992-01-28 00:00:00

abstract：:Genomic clones encompassing all the translated sequences, the 3' untranslated sequence, and 1 kb flanking the ATG translation initiation codon of bovine tropoelastin have been obtained and characterized by restriction enzyme analysis and extensive DNA sequencing. These analyses demonstrated that functionally distinct ...

journal_title：Biochemistry

authors： Yeh H,Anderson N,Ornstein-Goldstein N,Bashir MM,Rosenbloom JC,Abrams W,Indik Z,Yoon K,Parks W,Mecham R

更新日期：1989-03-21 00:00:00

abstract：:Efforts to characterize the receptor recognition domain of alpha-macroglobulins have primarily focused on human alpha 2-macroglobulin (alpha 2M). In the present work, the structure and function of the alpha-macroglobulin receptor recognition site were investigated by amino acid sequence analysis, plasma clearance, and...

journal_title：Biochemistry

authors： Enghild JJ,Thøgersen IB,Roche PA,Pizzo SV

更新日期：1989-02-07 00:00:00

abstract：:Human S100B(beta beta) is a small intracellular EF-hand calcium-binding protein that consists of two noncovalently associated 91-residue beta monomers. The three-dimensional structures of S100B reveal the dimer interface consists of four alpha-helices (I, I' and IV, IV') packed in an X-type bundle. In this study, guan...

journal_title：Biochemistry

authors： Ferguson PL,Shaw GS

更新日期：2002-03-19 00:00:00

abstract：:Homodinuclear (Pt,Pt) and heterodinuclear (Ru,Pt) metal compounds having the generalized formula M(a)NH2(CH)4NH2M(b) are shown to form specific DNA lesions which can efficiently cross-link proteins to DNA. In this study, the homodinuclear case is represented by M(a) = M(b) = [cis-Pt(Cl2)-(NH3)] and the heterodinuclear...

journal_title：Biochemistry

authors： Van Houten B,Illenye S,Qu Y,Farrell N

更新日期：1993-11-09 00:00:00

abstract：:Parathyroid hormone (PTH) regulates calcium and phosphate metabolism through a G-protein-coupled receptor which is shared with PTH-related protein (PTHrP). Therefore, structure-activity studies of PTH and PTHrP with their common receptor provide an unusual opportunity to examine the structural elements in the two horm...

journal_title：Biochemistry

authors： Nakamoto C,Behar V,Chin KR,Adams AE,Suva LJ,Rosenblatt M,Chorev M

更新日期：1995-08-22 00:00:00

abstract：:The selenoamino acids methylselenocysteine (MeSeCys) and selenomethionine (SeMet) have disparate efficacies as anticancer agents. Herein, we use X-ray absorption spectroscopy to determine the chemical form of selenium in human neuroblastoma cells. Cells treated with MeSeCys contain a significant diselenide component, ...

journal_title：Biochemistry

authors： Weekley CM,Aitken JB,Musgrave IF,Harris HH

更新日期：2012-01-24 00:00:00

abstract：:The Salmonella typhimurium leu-500 promoter is active only in topA strains. In an earlier study (Chen, D., Bowater, R., Dorman, C., & Lilley, D. M. J. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 8784-8788), we showed that the activity of this promoter on a circular plasmid is a function of the transcription and translati...

journal_title：Biochemistry

authors： Chen D,Bowater RP,Lilley DM

更新日期：1993-12-07 00:00:00

abstract：:Metal homeostasis and resistance in bacteria is maintained by a panel of metal-sensing transcriptional regulators that collectively control transition metal availability and mediate resistance to heavy metal xenobiotics, including As(III), Cd(II), Pb(II), and Hg(II). The ArsR family constitutes a superfamily of metal ...

journal_title：Biochemistry

authors： Wang Y,Kendall J,Cavet JS,Giedroc DP

更新日期：2010-08-10 00:00:00

abstract：:Guanine nucleotide dissociation stimulator (GDS) promotes the release of tightly bound GDP from various Ras superfamily proteins, including RhoA, Rac1, K-Ras, Rap1A, and Rap1B. It displays no significant sequence homology to other known exchange factors for small G-proteins. Studies are reported here of the mechanism ...

journal_title：Biochemistry

authors： Hutchinson JP,Eccleston JF

更新日期：2000-09-19 00:00:00

abstract：:A description of the efficient high-level expression of the monomer hemoglobin (GMG4) from Glycera dibranchiata is presented. The cDNA described by Simons and Satterlee [Simons, P.C., & Satterlee, J.D. (1989) Biochemistry 28, 8525-8530] was subcloned into an expression system, and conditions were found that led to the...

journal_title：Biochemistry

authors： Alam SL,Dutton DP,Satterlee JD

更新日期：1994-08-30 00:00:00

abstract：:12-Iodo-cis-9-octadecenoic acid (12-IODE) is a time-dependent, irreversible inactivator of soybean lipoxygenase 1. The rate of inactivation is independent of 12-IODE concentration above 20 microM and is half-maximal at about 4 microM. Inactivation by 12-IODE requires lipid hydroperoxide, which must be present even aft...

journal_title：Biochemistry

authors： Rotenberg SA,Grandizio AM,Selzer AT,Clapp CH

更新日期：1988-11-29 00:00:00

abstract：:Neutral oligosaccharides, which accounted for 74% of the total N-linked sugar chains released by hydrazinolysis of rat small intestinal aminopeptidase N, were investigated on a structural basis. They are mainly composed of complex-type sugar chains with tri- and tetraantennary structures, and small amounts of high man...

journal_title：Biochemistry

authors： Takasaki S,Erickson RH,Kim YS,Kochibe N,Kobata A

更新日期：1991-09-17 00:00:00

abstract：:The structures of ternary complexes of human inositol monophosphatase with inhibitory Gd3+ and either D- or L-myo-inositol 1-phosphate have been determined to 2.2-2.3 A resolution using X-ray crystallography. Substrate and metal are bound identically in each active site of the phosphatase dimer. The substrate is prese...

journal_title：Biochemistry

authors： Bone R,Frank L,Springer JP,Pollack SJ,Osborne SA,Atack JR,Knowles MR,McAllister G,Ragan CI,Broughton HB

更新日期：1994-08-16 00:00:00

abstract：:High-risk human papillomavirus E6 participates in tumorigenic progression, mainly by its ability to promote p53 degradation. HPV transcripts show a complex splicing pattern, where E6* is the most abundant transcript in high-risk HPV types, comprising the first 50 amino acids of E6. No structural or biochemical informa...

journal_title：Biochemistry

authors： Heer A,Alonso LG,de Prat-Gay G

更新日期：2011-03-01 00:00:00

abstract：:A double mutant of the single-domain protein barstar having a single tryptophan (W53) was made by mutating the remaining two tryptophans (W38 and W44) into phenylalanines. W53 is buried in the core of barstar. Time-resolved fluorescence of the mutant barstar (W38FW44F) showed that W53 has a single fluorescence lifetim...

journal_title：Biochemistry

authors： Swaminathan R,Nath U,Udgaonkar JB,Periasamy N,Krishnamoorthy G

更新日期：1996-07-16 00:00:00