Methylselenocysteine treatment leads to diselenide formation in human cancer cells: evidence from X-ray absorption spectroscopy studies.

abstract：:The dehaloperoxidase-hemoglobin (DHP) from the terebellid polychaete Amphitrite ornata is a multifunctional hemoprotein that catalyzes the oxidation of a wide variety of substrates, including halo/nitrophenols, haloindoles, and pyrroles, via peroxidase and/or peroxygenase mechanisms. To probe whether substrate substit...

journal_title：Biochemistry

authors： McGuire AH,Carey LM,de Serrano V,Dali S,Ghiladi RA

更新日期：2018-07-31 00:00:00

abstract：:The complex of tropomyosin and troponin binds to actin and inhibits activation of myosin ATPase activity and force production of striated muscles at low free Ca(2+) concentrations. Ca(2+) stimulates ATP activity, and at subsaturating actin concentrations, the binding of NEM-modified S1 to actin-tropomyosin-troponin in...

journal_title：Biochemistry

authors： Gafurov B,Fredricksen S,Cai A,Brenner B,Chase PB,Chalovich JM

更新日期：2004-12-07 00:00:00

abstract：:The fluorescent nucleotide analogue of ADP, 1,N6-ethenoadenosine diphosphate (epsilon ADP), has been used to probe the active site of myosin subfragment 1 (SF1). The Mg complex of ADP was shown to be trapped stoichiometrically at the active site by a variety of thiol cross-linking agents having sulfur to sulfur spanni...

journal_title：Biochemistry

authors： Perkins WJ,Wells JA,Yount RG

更新日期：1984-08-14 00:00:00

abstract：:We have studied the transient kinetics of electron transfer from a positively charged viologen analogue (propylene diquat), reduced by pulsed laser excitation of the deazariboflavin/EDTA system, to the net negatively charged ferredoxins from spinach and Clostridium pasteurianum. Spinach ferredoxin showed monophasic ki...

journal_title：Biochemistry

authors： Navarro JA,Cheddar G,Tollin G

更新日期：1989-07-11 00:00:00

abstract：:By use of two-dimensional NMR techniques most of the proton resonances (greater than 90%) are assigned for the tryptic digest fragment 96-133 of bovine somatotropin in 30% 2,2,2-trifluoroethanol-d3/70% H2O. Qualitative analysis of the nuclear Overhauser enhancement (NOE) data indicates that a region of alpha-helix spa...

journal_title：Biochemistry

authors： Gooley PR,MacKenzie NE

更新日期：1988-05-31 00:00:00

abstract：:Signal transduction in B cells is mediated, in part, by the interaction of the cytoplasmic components of the antigen receptor complex and various members of the src family tyrosine kinases. Key to this process appears to be the interaction of the tyrosine kinase SH2 domains with the tyrosine-phosphorylated cytoplasmic...

journal_title：Biochemistry

authors： Metzler WJ,Leiting B,Pryor K,Mueller L,Farmer BT 2nd

更新日期：1996-05-21 00:00:00

abstract：:H1 can rearrange in chicken erythrocyte polynucleosomes in 80 mM NaCl buffers. These rearrangements have been studied by sedimentation analysis. H1 redistributes between polynucleosomes as well as between polynucleosomes and monosomes. In these rearrangements H1 molecules move to free DNA sites. In contrast to H1, the...

journal_title：Biochemistry

authors： Lasters I,Muyldermans S,Wyns L,Hamers R

更新日期：1981-03-03 00:00:00

abstract：:A new orally active oxidosqualene:lanosterol cyclase (OSLC) inhibitor (Ro48-8071; Morand, O. H. et al. (1997) J. Lipid Res. 38, 373-390) showed potent noncompetitive inhibition of bacterial squalene:hopene cyclase (SHC) from Alicyclobacillus acidocaldarius (IC50 = 9.0 nM, KI = 6.6 nM) and OSLC (IC50 = 40 nM, KI = 22 n...

journal_title：Biochemistry

authors： Abe I,Zheng YF,Prestwich GD

更新日期：1998-04-28 00:00:00

abstract：:The effect of hydrostatic pressure upon solutions of chymotrypsinogen and lysozyme at room temperature has been followed by employing a new technique [Chryssomallis, G. S., Drickamer, H. G., & Weber, G. (1978) J. Appl. Phys. 49, 3084] that permits the measurement of fluorescence polarization at pressures of up to 10 k...

journal_title：Biochemistry

authors： Chryssomallis GS,Torgerson PM,Drickamer HG,Weber G

更新日期：1981-07-07 00:00:00

abstract：:The requirement of cytochrome c-550 (PSII-V) in photosystem II (PSII) has been assessed in Synechocystis sp. PCC 6803 containing mutations between Gly-351 and Thr-436 of the loop E domain of the chlorophyll a-binding protein CP47. Six photoautotrophic strains were utilized to compare the effect of removal of either th...

journal_title：Biochemistry

authors： Morgan TR,Shand JA,Clarke SM,Eaton-Rye JJ

更新日期：1998-10-13 00:00:00

abstract：:Several furanocoumarins were tested for their ability to inhibit human P450 2A6 activity. The metabolites and conjugates formed from these furanocoumarins after incubation with reconstituted purified P450 2A6 in the absence and presence of exogenous nucleophiles were characterized by UV and LC/ESI-MS/MS analysis. The ...

journal_title：Biochemistry

authors： Koenigs LL,Trager WF

更新日期：1998-07-14 00:00:00

abstract：:We have measured the hydration of CO2 catalyzed by bovine red cell carbonic anhydrase in mixtures of H2O and D2O at pH(D) which is in the plateau region of the pH--activity profile. The steady-state parameters for hydration Vmax and Km both show an exponential dependence on the atom fraction of deuterium in solvent wa...

journal_title：Biochemistry

authors： Venkatasubban KS,Silverman DN

更新日期：1980-10-28 00:00:00

abstract：:One cause of multidrug resistance is the overexpression of P-glycoprotein, a 170 kDa plasma membrane ABC transporter, which functions as an ATP-driven efflux pump with broad specificity for hydrophobic drugs, peptides, and natural products. The protein appears to interact with its substrates within the membrane enviro...

journal_title：Biochemistry

authors： Lugo MR,Sharom FJ

更新日期：2005-01-18 00:00:00

abstract：:Recycling of yeast fumarase to permit repetition of its reaction chemistry requires two proton transfers and two conformational changes, in pathways that are different in detail but thematically similar in the two directions. In the malate --> fumarate direction, simple anions such as acetate accelerate the fumarate-o...

journal_title：Biochemistry

authors： Rose IA

更新日期：1998-12-22 00:00:00

abstract：:The bile acid cholate disrupts platelet plasma membrane vesicles and inactivates 5-hydroxytryptamine transport activity. The transporter appears not to be solubilized by this procedure, but remains associated with a high molecular weight aggregate. Merely removing cholate does not restore the vesicular structure or tr...

journal_title：Biochemistry

authors： Rudnick G,Nelson PJ

更新日期：1978-11-28 00:00:00

abstract：:The stimulatory GTP-binding protein of adenylate cyclase, Gs, and beta-adrenergic receptors were reconstituted into unilamellar phospholipid vesicles. The kinetics of the quasiirreversible binding of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) to Gs, equivalent to Gs activation by nucleotide, was studied with re...

journal_title：Biochemistry

authors： Asano T,Ross EM

更新日期：1984-11-06 00:00:00

abstract：:Gene targeting by triplex-forming oligonucleotides (TFOs) has proven useful for gene modulation in vivo. Photoreactive molecules have been conjugated to TFOs to direct sequence-specific damage in double-stranded DNA. However, the photoproducts are often repaired efficiently in cells. This limitation has led to the sea...

journal_title：Biochemistry

authors： Benfield AP,Macleod MC,Liu Y,Wu Q,Wensel TG,Vasquez KM

更新日期：2008-06-10 00:00:00

abstract：:Chloride intracellular channel protein 1 (CLIC1) functions as an anion channel in plasma and nuclear membranes when its soluble monomeric form converts to an integral-membrane form. The transmembrane region of CLIC1 is located in its thioredoxin-like domain 1, but the mechanism whereby the protein converts to its memb...

journal_title：Biochemistry

authors： Stoychev SH,Nathaniel C,Fanucchi S,Brock M,Li S,Asmus K,Woods VL Jr,Dirr HW

更新日期：2009-09-08 00:00:00

abstract：:Mutations in the hydrophobic interior of proteins are generally thought to weaken the interactions only in their immediate neighborhood. This forms the basis of protein engineering-based studies of folding mechanism and function. However, mutational work on diverse proteins has shown that distant residues are thermody...

journal_title：Biochemistry

authors： Rajasekaran N,Suresh S,Gopi S,Raman K,Naganathan AN

更新日期：2017-01-10 00:00:00

abstract：:Murine fibroblasts cultured in the presence of fibroblast growth factor-1 express relatively high levels of FR-1, a approximately 36 kDa protein related to the aldo-keto reductase superfamily [Donohue, P. J., Alberts, G. F., Hampton, B. S., Winkles, J. A. (1994) J. Biol. Chem. 269, 8604-8609]. While the crystal struct...

journal_title：Biochemistry

authors： Srivastava S,Harter TM,Chandra A,Bhatnagar A,Srivastava SK,Petrash JM

更新日期：1998-09-15 00:00:00

abstract：:We previously showed that estrogen-related receptor alpha1 (ERRalpha1) can compete with estrogen receptor alpha (ERalpha) for binding to estrogen response elements (EREs), repressing transcription in the mammary carcinoma cell line MCF-7. Given that ERRalpha1 can function in the absence of ligands and exists as a phos...

journal_title：Biochemistry

authors： Vu EH,Kraus RJ,Mertz JE

更新日期：2007-08-28 00:00:00

abstract：:Cholera toxin, an activator of adenylate cyclase in a wide variety of cells, is a substrate for the phosphotransferase reaction catalyzed by purified cyclic adenosine 5'-monophosphate dependent bovine cardiac muscle protein kinase and the protein associated with human erythrocyte membranes. Phosphorylation occurs when...

journal_title：Biochemistry

authors： Rosen OM

更新日期：1976-06-29 00:00:00

abstract：:Pyruvate phosphate dikinase catalyzes the interconversion of adenosine 5'-triphosphate (ATP), orthophosphate (P(i)), and pyruvate with adenosine 5'-monophosphate (AMP), pyrophosphate (PP(i)), and phosphoenolpyruvate (PEP). The Arg 561 residue of Clostridium symbiosum PPDK is contained within a Gly-rich stretch of sequ...

journal_title：Biochemistry

authors： Yankie L,Xu Y,Dunaway-Mariano D

更新日期：1995-02-21 00:00:00

abstract：:Interaction of affinity chromatographically purified antihistone H3 and antihistone H4 with isolated HeLa core particles, followed by separation of unreacted and reacted particles by sedimentation, demonstrates that every core particle contains these histones. Taken together with our previous data indicating the prese...

journal_title：Biochemistry

authors： Bustin M,Simpson RT,Sperling R,Goldblatt D

更新日期：1977-11-29 00:00:00

abstract：:Two paramagnetic probes, viz., Mn2+ and Cr3+-ATP, were used to map distances to various loci on carbamoyl-phosphate synthetase by using NMR measurements. The paramagnetic influence of Mn2+ on the 1H of L-glutamate and L-ornithine was measured at 200 and 360 MHz. On the basis of these data, a correlation time for the p...

journal_title：Biochemistry

authors： Raushel FM,Anderson PM,Villafranca JJ

更新日期：1983-04-12 00:00:00

abstract：:The EcoRV DNA-(adenine-N6)-methyltransferase (MTase) recognizes GATATC sequences and modifies the first adenine residue within this site. Parts of its DNA interface show high sequence homology to DNA MTases of the dam family which recognize and modify GATC sequences. A phylogenetic analysis of M.EcoRV and dam-MTases s...

journal_title：Biochemistry

authors： Beck C,Cranz S,Solmaz M,Roth M,Jeltsch A

更新日期：2001-09-18 00:00:00

abstract：:In Sphingomonas CHY-1, a single ring-hydroxylating dioxygenase is responsible for the initial attack of a range of polycyclic aromatic hydrocarbons (PAHs) composed of up to five rings. The components of this enzyme were separately purified and characterized. The oxygenase component (ht-PhnI) was shown to contain one R...

journal_title：Biochemistry

authors： Jouanneau Y,Meyer C,Jakoncic J,Stojanoff V,Gaillard J

更新日期：2006-10-10 00:00:00

abstract：:Inactivation of rabbit lung angiotensin converting enzyme (ACE) by 1-fluoro-2,4-dinitrobenzene (Dnp-F) has been shown to be due primarily to the modification of a tyrosine residue [Bünning, P., Kleeman, S.G., & Riordan, J.F. (1990) Biochemistry (preceding paper in this issue)]. Rabbit testicular ACE is also inactivate...

journal_title：Biochemistry

authors： Chen YN,Riordan JF

更新日期：1990-11-20 00:00:00

abstract：:Site-directed mutagenesis was used to construct mutant Trp repressors with each of the 38 possible single amino acid changes of the first 2 amino acid residues (Ile79 and Ala80) in the second "recognition" alpha-helix of the helix-turn-helix DNA-binding motif. Eight of these mutant repressors with Ile79 and Ala80 chan...

journal_title：Biochemistry

authors： Pfau J,Arvidson DN,Youderian P,Pearson LL,Sigman DS

更新日期：1994-09-20 00:00:00

abstract：:The search for a rational method of developing an antimalarial vaccine (malaria caused by Plasmodium falciparum) consists of blocking receptor-ligand interaction. Conserved peptides derived from proteins involved in invasion and having strong red blood cell binding ability have thus been identified; immunization studi...

journal_title：Biochemistry

authors： Patarroyo ME,Alba MP,Vargas LE,Silva Y,Rosas J,Rodríguez R

更新日期：2005-05-10 00:00:00