Abstract:
:Ethylene and its analogues acetylene, carbon monoxide, and propylene inhibited the rate of oxidation of indole-3-acetic acid by peroxidase. Annulment of this effect by addition of superoxide dismutase showed that inhibition occurred only in the presence of the superoxide anion radical (O2-.). Kinetic and spectral data established that ethylene and its analogues enhanced markedly the rate of reaction of O2-. with peroxidase. This reaction resulted in the formation of compound III, an oxy-ferrous complex of peroxidase. In the presence of indole-3-acetic acid, the interaction between ethylene, peroxidase, and O2-. activated the reduced peroxidase in equilibrium compound III shuttle. O2-. is a major product of this shuttle, and compound III constitutes the dominant steady-state form of peroxidase. These interactions may help to explain the mechanism of action of ethylene as a plant growth regulator.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Smith AM,Morrison WL,Milham PJdoi
10.1021/bi00276a019subject
Has Abstractpub_date
1983-03-29 00:00:00pages
1645-50issue
7eissn
0006-2960issn
1520-4995journal_volume
22pub_type
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