Mannitol-specific carrier protein from the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system can be extracted as a dimer from the membrane.

abstract：:The human antioxidant protein, HAH1, is an important participant in a Cu(I) transport chain, delivering one Cu(I) ion to the Wilson's (WND) or Menkes disease protein (MNK). Full geometry optimizations and second-derivative calculations were performed on several binding site models using the B3LYP functional to derive ...

journal_title：Biochemistry

authors： Op't Holt BT,Merz KM Jr

更新日期：2007-07-31 00:00:00

abstract：:The malaria parasite pigment, hemozoin, is a crystal of ferriprotoporphyrin IX (FP-Fe(III)), a product of hemoglobin digestion. Hemozoin formation is essential for FP-Fe(III) detoxification in the parasite; it is the main target of quinoline antimalarials and can modulate immune and inflammation responses. To gain fur...

journal_title：Biochemistry

authors： Klonis N,Dilanian R,Hanssen E,Darmanin C,Streltsov V,Deed S,Quiney H,Tilley L

更新日期：2010-08-10 00:00:00

abstract：:The crystal structure of the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) has been reported recently [Lieberman, R. L., and Rosenzweig, A. C. (2005) Crystal structure of a membrane-bound metalloenzyme that catalyses the biological oxidation of methane, Nature 434, 177-182]. Subsequent ...

journal_title：Biochemistry

authors： Yu SS,Ji CZ,Wu YP,Lee TL,Lai CH,Lin SC,Yang ZL,Wang VC,Chen KH,Chan SI

更新日期：2007-12-04 00:00:00

abstract：:Maduropeptin (MDP) is a recently isolated antitumor antibiotic, consisting of an enediyne-containing chromophore embedded in a highly acidic protein. This holoantibiotic damages duplex DNA in vitro, producing a mixture of single- and double-strand breaks at selected sites. The chromophore, isolated as the methanol add...

journal_title：Biochemistry

authors： Zein N,Solomon W,Colson KL,Schroeder DR

更新日期：1995-09-12 00:00:00

abstract：:Bacteriocins are bacterial peptides with specific activity against competing species. They hold great potential as natural preservatives and for their probiotic effects. We show here nuclear magnetic resonance-based evidence that glycocin F, a 43-amino acid bacteriocin from Lactobacillus plantarum, contains two β-link...

journal_title：Biochemistry

authors： Venugopal H,Edwards PJ,Schwalbe M,Claridge JK,Libich DS,Stepper J,Loo T,Patchett ML,Norris GE,Pascal SM

更新日期：2011-04-12 00:00:00

abstract：:Porcine pancreatic phospholipase A2 interacts with micelles of the substrate analogue n-octadecylphosphocholine to form a specific complex over considerably wide concentration ranges of both lipid and protein. UV absorption difference spectroscopy measurements indicate that the ratio of lipid to protein molecules in t...

journal_title：Biochemistry

authors： Hille JD,Donné-Op den Kelder GM,Sauve P,de Haas GH,Egmond MR

更新日期：1981-07-07 00:00:00

abstract：:We report on the determination of the solution structure of two sequence-related oligonucleotides, d(GTACGTAC)2 and d(CATCGATG)2. Results have been obtained by using a combined approach of (a) two-dimensional NMR, including proton and phosphorus experiments, (b) restrained molecular mechanics performed with sugar phas...

journal_title：Biochemistry

authors： Lefebvre A,Mauffret O,Hartmann B,Lescot E,Fermandjian S

更新日期：1995-09-19 00:00:00

abstract：:A single amino acid substitution from methionine-184 to valine (M184V) of HIV-1 reverse transcriptase (RT) evokes the 1000-fold 3TC (Lamivudine) resistance by the HIV-1 virus observed in the clinic. The M184V mutant HIV-1 RT was studied to assess its catalytic efficiency during single nucleotide incorporation using a ...

journal_title：Biochemistry

authors： Feng JY,Anderson KS

更新日期：1999-07-20 00:00:00

abstract：:LL-23 is a natural peptide corresponding to the 23 N-terminal amino acid residues of human host defense cathelicidin LL-37. LL-23 demonstrated, compared to LL-37, a conserved ability to induce the chemokine MCP-1 in human peripheral blood mononuclear cells, a lack of ability to suppress induction of the pro-inflammato...

journal_title：Biochemistry

authors： Wang G,Elliott M,Cogen AL,Ezell EL,Gallo RL,Hancock RE

更新日期：2012-01-17 00:00:00

abstract：:We developed a general strategy for labeling expressed membrane proteins with a peptide epitope tag and detecting the tagged proteins in native cellular membranes. First, we genetically encoded the unnatural amino acid p-azido-L-phenylalanine (azF) at various specific sites in a G protein-coupled receptor (GPCR), C-C ...

journal_title：Biochemistry

authors： Naganathan S,Ye S,Sakmar TP,Huber T

更新日期：2013-02-12 00:00:00

abstract：:Single-stranded microRNAs (miRNAs) regulate gene expression by triggering mRNA degradation and/or inhibiting mRNA translation. miRNAs play important roles in various critical cellular processes and are associated with numerous human diseases, including cancer and neurodegenerative diseases. miRNA sequences are embedde...

journal_title：Biochemistry

authors： Nguyen HM,Nguyen TD,Nguyen TL,Nguyen TA

更新日期：2019-01-29 00:00:00

abstract：:Hydrogen bonds which form between a hydrogen bond donor and an aromatic ring as acceptor are thought to contribute to the stability and function of proteins. We have tested the function of such an interaction in a highly homologous pair of proteins, cellular retinol-binding protein (CRBP) and cellular retinol-binding ...

journal_title：Biochemistry

authors： Jamison RS,Kakkad B,Ebert DH,Newcomer ME,Ong DE

更新日期：1995-09-05 00:00:00

abstract：:The three catalytic sites of the F(O)F(1) ATP synthase interact through a cooperative mechanism that is required for the promotion of catalysis. Replacement of the conserved alpha subunit Arg-376 in the Escherichia coli F(1) catalytic site with Ala or Lys resulted in turnover rates of ATP hydrolysis that were 2 x 10(3...

journal_title：Biochemistry

authors： Le NP,Omote H,Wada Y,Al-Shawi MK,Nakamoto RK,Futai M

更新日期：2000-03-14 00:00:00

abstract：:We have studied the effects of site-directed mutations in Escherichia coli nitrate reductase A (NarGHI) on heme reduction by a menaquinol analogue (menadiol) using the stopped-flow method. For NarGHI(H66Y) and NarGHI(H187Y), both lacking heme b(L) but having heme b(H), the heme reduction by menadiol is abolished. For ...

journal_title：Biochemistry

authors： Zhao Z,Rothery RA,Weiner JH

更新日期：2003-12-09 00:00:00

abstract：:The contact between EcoRI endonuclease and the "primary clamp" phosphate of its recognition site pGAATTC is absolutely required for recognition of the canonical and all variant DNA sites. We have probed this contact using oligonucleotides containing single stereospecific (Rp)- or (Sp)- phosphorothioates (Ps). At the G...

journal_title：Biochemistry

authors： Kurpiewski MR,Koziolkiewicz M,Wilk A,Stec WJ,Jen-Jacobson L

更新日期：1996-07-09 00:00:00

abstract：:Although Mg(2+) is the cation that functions as the cofactor for the nucleotidyl transfer reaction for almost all DNA polymerases, Mn(2+) can also serve, but when it does, the degree of base discrimination exhibited by most DNA polymerases (pols) is diminished. Metal ions other than Mg(2+) or Mn(2+) can also act as co...

journal_title：Biochemistry

authors： Vashishtha AK,Konigsberg WH

更新日期：2016-05-10 00:00:00

abstract：:The conformational constraints for apoA-I bound to recombinant phospholipid complexes (rHDL) were attained from a combination of chemical cross-linking and mass spectrometry. Molecular distances were then used to refine models of lipid-bound apoA-I on both 80 and 96 A diameter rHDL particles. To obtain molecular const...

journal_title：Biochemistry

authors： Bhat S,Sorci-Thomas MG,Tuladhar R,Samuel MP,Thomas MJ

更新日期：2007-07-03 00:00:00

abstract：:The membrane-bound state of the gene 9 minor coat protein of bacteriophage M13 was studied in various membrane-mimicking systems, including organic solvents, detergent micelles, and phospholipid bilayers. For this purpose we determined the conformational and aggregational properties of the chemically synthesized prote...

journal_title：Biochemistry

authors： Houbiers MC,Spruijt RB,Wolfs CJ,Hemminga MA

更新日期：1999-01-19 00:00:00

abstract：:The Salmonella typhimurium PhoN protein is a nonspecific acid phosphatase and belongs to the phosphatidic acid phosphatase type 2 (PAP2) superfamily. We report here the crystal structures of phosphate-bound PhoN, the PhoN-tungstate complex, and the T159D mutant of PhoN along with functional characterization of three m...

journal_title：Biochemistry

authors： Makde RD,Mahajan SK,Kumar V

更新日期：2007-02-27 00:00:00

abstract：:Cathepsin L, a lysosomal cysteine protease, is overexpressed and secreted by malignantly transformed cells. However, the reason for secretion of this man 6-phosphate-containing lysosomal protease into the extracellular medium is not clear. We wished to determine whether there is a region within the primary sequence of...

journal_title：Biochemistry

authors： Chauhan SS,Ray D,Kane SE,Willingham MC,Gottesman MM

更新日期：1998-06-09 00:00:00

abstract：:The argument and experimental evidence are presented for a two-state model that explains the action of both helical and beta-sheet antimicrobial peptides after they bind to the plasma membranes of cells. Each peptide has two distinct physical states of binding to lipid bilayers. At low peptide-to-lipid ratios (P/L), t...

journal_title：Biochemistry

authors： Huang HW

更新日期：2000-07-25 00:00:00

abstract：:The use of derivatives of alpha-thrombin obtained by limited proteolysis, that have only a single peptide bond cleaved, allowed the unequivocal correlation between the change in covalent structure and alteration of the enzymatic properties. beta T-Thrombin contains a single cleavage in the surface loop corresponding t...

journal_title：Biochemistry

authors： Hofsteenge J,Braun PJ,Stone SR

更新日期：1988-03-22 00:00:00

abstract：:PhoR of Bacillus subtilis is a histidine sensor-kinase belonging to the family of two-component signal transduction systems. PhoR is responsible for processing the phosphate-starvation signal and providing phosphate input to regulate the level of phosphorylated response regulator, PhoP, which activates/represses Pho r...

journal_title：Biochemistry

authors： Shi L,Liu W,Hulett FM

更新日期：1999-08-03 00:00:00

abstract：:Nhp6A is an abundant non-histone chromatin-associated protein in Saccharomyces cerevisiae that contains a minor groove DNA binding motif called the HMG box. In this report, we show that Nhp6Ap binds to cisplatin intrastrand cross-links on duplex DNA with a 40-fold greater affinity than to unmodified DNA with the same ...

journal_title：Biochemistry

authors： Wong B,Masse JE,Yen YM,Giannikopoulos P,Feigon J,Johnson RC

更新日期：2002-04-30 00:00:00

abstract：:Understanding the mechanisms of free energy transfer in metabolism is fundamental to understanding how the chemical forces that sustain the molecular organization of the cell are distributed. Recent studies of molecular motors (1-3) and ATP-driven proton transport (4-6) describe how chemical potential is transferred a...

journal_title：Biochemistry

authors： Wei J,Tang QX,Varlamova O,Roche C,Lee R,Leyh TS

更新日期：2002-07-02 00:00:00

abstract：:We detect internal water molecules in a membrane-embedded receptor-transducer complex and demonstrate water structure changes during formation of the signaling state. Time-resolved FTIR spectroscopy reveals stimulus-induced repositioning of one or more structurally active water molecules to a significantly more hydrop...

journal_title：Biochemistry

authors： Bergo VB,Spudich EN,Spudich JL,Rothschild KJ

更新日期：2009-02-10 00:00:00

abstract：:Methenyltetrahydromethanopterin (methenyl-H(4)MPT(+)) cyclohydrolase (Mch) catalyzes the interconversion of methenyl-H(4)MPT(+) and formyl-H(4)MPT in the one-carbon energy metabolism of methanogenic, methanotrophic, and sulfate-reducing archaea and of methylotrophic bacteria. To understand the catalytic mechanism of t...

journal_title：Biochemistry

authors： Upadhyay V,Demmer U,Warkentin E,Moll J,Shima S,Ermler U

更新日期：2012-10-23 00:00:00

abstract：:Detection of specific reaction products is a powerful approach for dissecting out pathways that mediate oxidative damage in vivo. Eosinophil peroxidase (EPO), an abundant protein secreted from activated eosinophils, has been implicated in promoting oxidative tissue injury in conditions such as asthma, allergic inflamm...

journal_title：Biochemistry

authors： Wu W,Chen Y,d'Avignon A,Hazen SL

更新日期：1999-03-23 00:00:00

abstract：:The regulation of the rate-controlling enzyme in cholesterol biosynthesis and of the incorporation of [14C]oleate into cholesterol esters were studied in established lymphoid cell lines from normal subjects and compared with that of eight patients with genetic abnormalities of lipid metabolism. The activity of 3-hydro...

journal_title：Biochemistry

authors： Kayden HJ,Hatam L,Beratis NG

更新日期：1976-02-10 00:00:00

abstract：:Poly(ethylene glycol) (PEG) of average molecular weight 8000 was used to mediate the fusion of large unilamellar vesicles composed of dipalmitoylphosphatidylcholine. Fusion was monitored by fluorescence assays of lipid mixing and aqueous contents mixing. The extent of lipid mixing, as monitored by DPHpPC fluorescence ...

journal_title：Biochemistry

authors： Parente RA,Lentz BR

更新日期：1986-10-21 00:00:00