Abstract：
:To better understand the factors controlling the binding of apolipoprotein molecules at the surfaces of serum lipoprotein particles, the adsorption of human apolipoprotein A-I to phospholipid monolayers has been studied. The influence of lipid packing was investigated by spreading the monolayers at various initial surface pressures (pi i) and by using various types of lipid. The adsorption of 14C-methylated apolipoprotein A-I was monitored by simultaneously following the surface radioactivity (which could be converted to the surface concentration of protein, gamma) and the change in surface pressure (delta pi). In general, increasing the pi i of lipid monolayers reduces the adsorption of apolipoprotein A-I; for expanded egg phosphatidylcholine (PC) monolayers at pi i greater than or equal to 32 dyn/cm, gamma and delta pi are zero. The degree of adsorption of the apolipoprotein is also influenced by the physical state of the lipid monolayers. Thus, at a given pi i, apolipoprotein A-I adsorbs more to expanded monolayers than to condensed monolayers so that, at a given subphase concentration of protein, gamma of apolipoprotein A-I with various phospholipid monolayers decreases in the order egg PC greater than egg sphingomyelin greater than distearoyl-PC. The plot of gamma against pi i for adsorption of apolipoprotein A-I to dipalmitoylphosphatidylcholine (DPPC) monolayers shows an inflection at pi i = 8 dyn/cm; at this pi, the DPPC monolayer undergoes a phase transition from liquid (expanded) to solid (condensed) state. Addition of cholesterol generally decreases the adsorption of apolipoprotein A-I to egg PC monolayers.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Ibdah JA,Phillips MCdoi
10.1021/bi00418a073subject
Has Abstractpub_date
1988-09-06 00:00:00pages
7155-62issue
18eissn
0006-2960issn
1520-4995journal_volume
27pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The fate of plasma and nuclear membrane polypeptides in preparations of acidic chromosomal protein from chicken erythrocytes has been investigated. It is shown that detergent extraction procedures (Nonidet P-40, Triton X-100, and saponin), commonly employed in the preparation of acidic chromosomal protein, cannot be r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00670a034
更新日期：1976-12-14 00:00:00
abstract：:The inhibitory effects of urea on the normally rapid fibrillation of human calcitonin (hCT) were investigated by 1H NMR. From subtle differences in the chemical shift of hCT in the presence and absence of urea, the occurrence of weak interactions between urea and hCT was confirmed. The chemical shifts on the NH and C ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961013l
更新日期：1996-10-01 00:00:00
abstract：:The catalysis of the chemical exchange between acetaldehyde and its hydrate by bovine carbonic anhydrase B was investigated by the NMR line broadening and saturation transfer techniques. While both chemical exchange and binding to the enzyme had an effect on the line broadening of acetaldehyde and its hydrate, the sat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00550a021
更新日期：1980-04-29 00:00:00
abstract：:The partition of cis-parinaric acid (9,11,13,15-cis, trans, trans,cis-octadecatetraenoic acid, cis-PnA) and trans-parinaric acid (9,11,13,15-all-trans-octadecatetraenoic acid, trans-PnA) among aqueous, solid lipid, and fluid lipid phases has been measured by three spectroscopic parameters: absorption spectral shifts, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00576a012
更新日期：1979-05-01 00:00:00
abstract：:Distinct structural features of DNA, such as the curvature of dA tracts, are important in the recognition, packaging, and regulation of DNA. Physiologically relevant concentrations of magnesium have been found to enhance the curvature of dA tract DNAs, as monitored by solution-state NMR, indicating that the structure ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010853j
更新日期：2001-08-07 00:00:00
abstract：:New homo- and heterobifunctional cross-linking reagents have been synthesized. These reagents are based on ortho ester, acetal, and ketal functionalities that undergo acid-catalyzed dissociation but are base stable. The protein-reactive group in all the homobifunctional reagents is a maleimide group; the heterobifunct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00432a023
更新日期：1989-03-21 00:00:00
abstract：:ABCG2 is a half-ATP binding cassette (ABC) drug transporter that consists of a nucleotide binding domain (NBD) followed by a transmembrane domain. This half-ABC transporter is thought to form a homodimer in the plasma membrane where it transports anticancer drugs across the biological membranes in an ATP-dependent man...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900675v
更新日期：2009-09-29 00:00:00
abstract：:The left-handed polyproline II (P(II)) helix is a structure that has been given a great deal of attention lately because of its role in a wide variety of physiologically important processes and potential significance in protein unfolded states. Recent work by several authors has shown that residues besides proline can...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049922v
更新日期：2004-05-18 00:00:00
abstract：:We investigated how the intrinsic sequence-dependent properties probed via the phosphate linkages (BI ↔ BII equilibrium) influence the preferred shape of free DNA, and how this affects the nucleosome formation. First, this exploits NMR solution studies of four B-DNA dodecamers that together cover 39 base pairs of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500504y
更新日期：2014-09-09 00:00:00
abstract：:Cytochromes c require covalent attachment of heme via two thioether bonds at conserved CXXCH motifs, a process accomplished in prokaryotes by eight integral membrane proteins (CcmABCDEFGH), termed System I. Heme is trafficked from inside the cell to outside (via CcmABCD) and chaperoned (holoCcmE) to the cytochrome c s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00427
更新日期：2016-06-07 00:00:00
abstract：:The effects of cysteine modification and variations in pH on the equilibrium parameters for inducer and operator binding to the lactose repressor protein were examined. Operator binding affinity was minimally affected by increasing the pH from 7.5 to 9.2, whereas inducer binding was decreased for both the unliganded p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00367a020
更新日期：1986-09-23 00:00:00
abstract：:The interaction of the 20-kDa pore-forming domain of colicin A with phospholipid vesicles was investigated by gel permeation chromatography, analytical centrifugation, and electron microscopy. Under the experimental conditions of this study, this peptide was found to interact only with vesicles containing negatively c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00445a029
更新日期：1989-09-19 00:00:00
abstract：:Membrane preparations from two independent ouabain-resistant HeLa cell clones, HI-B1 and HI-C1, each appear to contain two species of (Na,K)ATPase. Two-thirds of the total (Na,K)ATPase in each mutant is indistinguishable from the enzyme in preparations of wild type cells with respect to ouabain binding, ouabain inhibi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00642a600
更新日期：1977-11-15 00:00:00
abstract：:Proteins from the enhanced intracellular survival (Eis) family are versatile acetyltransferases that acetylate amines at multiple positions of several aminoglycosides (AGs). Their upregulation confers drug resistance. Homologues of Eis are present in diverse bacteria, including many pathogens. Eis from Mycobacterium t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00244
更新日期：2015-05-26 00:00:00
abstract：:The unfolded state and flexible linkers in the folded structure play essential roles in protein stability and folding and protein-protein interactions. Intrinsic to these roles is the fact that unfolded proteins and flexible linkers sample many different conformations. Polymer models may capture this and complement ex...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi036269n
更新日期：2004-03-02 00:00:00
abstract：:In this communication, we present data which describe optimum conditions for reverse transcription of large ribonucleic acid (RNA) templates into deoxyribonucleic acid (DNA) transcripts by the avian retrovirus reverse transcriptase in vitro. In contrast to previous studies, we have optimized all of the reaction compon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00544a019
更新日期：1980-02-05 00:00:00
abstract：:The electron paramagnetic resonance (EPR) spectra of spin-labeled DNA duplexes, both bound to DEAE-Sephadex and free in solution, have been analyzed. The nitroxide spin-labels are covalently linked to a deoxyuridine residue using either a monoacetylene or diacetylene tether. This difference in tether length produces a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00013a028
更新日期：1995-04-04 00:00:00
abstract：:Many drugs are oxidized by membrane protein cytochrome P450 (CYP) enzymes during their metabolism process. CYPs are located mainly in endoplasmic reticulum (ER) membranes. Recent studies have suggested that CYP substrate drugs first bind the lipid bilayers of ER membranes and then the drugs reach the active site of CY...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00286
更新日期：2016-07-19 00:00:00
abstract：:Rat brain and kidney cDNA libraries were constructed and screened with a cDNA insert corresponding to the mRNA for the sheep kidney Na+,K+-ATPase catalytic subunit. The alpha-subunit cDNAs isolated from the kidney library were derived from a single class of messenger RNA, and the brain cDNAs were derived from three cl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00373a001
更新日期：1986-12-16 00:00:00
abstract：:Nitrogenase catalyzes the reduction of N2 to NH3, supporting all biological nitrogen fixation. Electron donors to this enzyme are ferredoxin or flavodoxin (in vivo) and sodium dithionite (in vitro). Features of these electron donors put a limit on spectrophotometric studies and electrocatalytic applications of nitroge...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00844
更新日期：2019-11-19 00:00:00
abstract：:Synthetic photocontrolled proteins could be powerful tools for probing cellular chemistry. Several previous attempts to produce such systems by incorporating photoisomerizable chromophores into biomolecules have led to photocontrol but with incomplete reversibility, where the chromophore becomes trapped in one photois...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060142r
更新日期：2006-05-16 00:00:00
abstract：:13C NMR spectroscopy has been used to study the amino group environments and metal binding properties of 13C reductively methylated bovine alpha-lactalbumin. Bovine alpha-lactalbumin is a Ca2+ metalloprotein containing 12 lysyl amino groups and a free amino terminus. All 13 amino groups can be 13C-dimethylated without...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00315a026
更新日期：1984-09-25 00:00:00
abstract：:The cyclic decapeptide antibiotic tyrocidine A was studied by two relatively new methods, viz., correlation proton magnetic resonance (pmr) spectroscopy and double-resonance difference pmr spectroscopy. The correlation method of spectral accumulation provided pmr spectra of good resolution, and in addition the signal-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00673a032
更新日期：1975-01-28 00:00:00
abstract：:Tyrosine hydroxylase (TH) catalyzes the first step in the biosynthesis of catecholamines. Regulation of TH enzyme activity is controlled through the posttranslational modification of its regulatory domain. The regulatory domain of TH can be phosphorylated at four serines (8, 19, 31, and 40) by a variety of protein kin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7019468
更新日期：2008-02-12 00:00:00
abstract：:Proteins from thermophiles possess high thermostability. The stabilization mechanisms differ between archaeal and bacterial proteins, whereby archaeal proteins are mainly stabilized via hydrophobic interactions and bacterial proteins by ion pairs. High stability is an important factor in promoting protein evolution, b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400652c
更新日期：2013-07-16 00:00:00
abstract：:G protein-coupled receptor (GPCR) kinases (GRKs) instigate the desensitization of activated GPCRs via phosphorylation that promotes interaction with arrestins, thereby preventing the interaction of GPCRs with heterotrimeric G proteins. A current proposed model of GRK1 activation involves the binding of activated rhodo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300295y
更新日期：2012-04-24 00:00:00
abstract：:β-Barrel membrane proteins are found in the outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria; however, exactly how they are folded and inserted remains unknown. Over the past decade, both functional and structural studies have greatly contributed to addressing this elusive mechanism. It is kno...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.5b00852
更新日期：2015-10-20 00:00:00
abstract：:Natriuretic peptide receptor A (NPRA) is a noncovalent homodimeric receptor, composed of an extracellular domain (ECD) with a ligand-binding site, a single transmembrane domain (TM), and an intracellular domain (ICD) exhibiting guanylyl cyclase activity. NPRA activation by atrial natriuretic peptide (ANP) leads to cGM...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901711w
更新日期：2010-06-08 00:00:00
abstract：:The Stk1/Stp1 and GraSR signal-transduction pathways are two distinct pathways in Staphylococcus aureus that rely on a reversible phosphorylation process in transducing external stimuli intracellularly. Stk1/Stp1 is an eukaryote-like Ser/Thr kinase phosphatase pair involved in purine biosynthesis, cell-wall metabolism...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401177n
更新日期：2013-11-12 00:00:00
abstract：:Introduction of specific structural probes into substrate binding sites of Escherichia coli glutamine synthetase is now possible. Various analogues of ATP substituted with an amino or sulfhydryl moiety at the 6- or 8-position of the purine ring have been found to substitute for ATP in the autoinactivation reaction of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00349a020
更新日期：1986-01-14 00:00:00
