当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Interaction between the antiapoptotic protein Nr-13 and cytochrome c. Antagonistic effect of the BH3 domain of Bax.

Interaction between the antiapoptotic protein Nr-13 and cytochrome c. Antagonistic effect of the BH3 domain of Bax.

Abstract：

:Mitochondria act as a focal point for upstream apoptosis signals by releasing cytochrome c into the cytosol, leading to the activation of caspases and subsequent cell death. Members of the Bcl-2 protein family regulate this phenomenon by heterodimerization via the BH3 domain of proapoptotic members opposing their pro- and antiapoptotic functions. The mechanism of cytochrome c release from mitochondria and of its regulation remains controversial. In vitro binding studies of purified and biologically active proteins should help in understanding the molecular mechanism of interactions and protein functions. In this work, the Bcl-2-related antiapoptotic chicken protein Nr-13 was overexpressed as a highly soluble recombinant protein which showed correct folding as judged by circular dichroism and fluorescence spectroscopy. Purified Nr-13 inhibits caspase-3 activation in a Xenopus egg-derived cell-free system, and neutralizes the proapoptotic activity of a synthetic peptide containing the BH3 domain of Bax. The latter effect correlates with the high-affinity binding of the BH3 peptide to Nr-13 as monitored by the intrinsic tryptophan fluorescence. On the basis of the structural similarity with Bcl-x(L), putative residues involved in this interaction were identified. Nr-13 exhibits a high-affinity interaction with cytochrome c which is prevented by preincubation with the BH3-Bax peptide. These findings are discussed with respect to a model for the regulation of apoptosis in which a direct interaction between the antiapoptotic protein and cytochrome c may prevent the apoptosis.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Moradi-Améli M,Lorca T,Ficheux D,di Pietro A,Gillet G

doi

10.1021/bi0110286

subject

Has Abstract

pub_date

2002-07-09 00:00:00

pages

8540-50

issue

27

eissn

0006-2960

issn

1520-4995

pii

bi0110286

journal_volume

41

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Altering the Enantioselectivity of Tyrosyl-tRNA Synthetase by Insertion of a Stereospecific Editing Domain.

    abstract：:Translation of mRNAs by the ribosome is stereospecific, with only l-amino acids being incorporated into the nascent polypeptide chain. This stereospecificity results from the exclusion of d-amino acids at three steps during protein synthesis: (1) the aminoacylation of tRNA by aminoacyl-tRNA synthetases, (2) binding of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b01167

    authors： Richardson CJ,First EA

    更新日期：2016-03-15 00:00:00

  • Isolation and characterization of titin T1 from bovine cardiac muscle.

    abstract：:Methods were developed for isolation of bovine cardiac titin in the apparently full-sized T1 form. The pure protein could be obtained either in the denatured state after chromatography of myofibril extracts on Sephacryl S-1000 or in the native state using hydroxylapatite chromatography. Titin-specific chicken polyclon...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00193a012

    authors： Pan KM,Damodaran S,Greaser ML

    更新日期：1994-07-12 00:00:00

  • Composition and sequence-dependent binding of RNA to the nucleocapsid protein of Moloney murine leukemia virus.

    abstract：:All retroviruses package two copies of their genomes during virus assembly, both of which are required for strand transfer-mediated recombination during reverse transcription. Genome packaging is mediated by interactions between the nucleocapsid (NC) domains of assembling Gag polyproteins and an RNA packaging signal, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047639q

    authors： Dey A,York D,Smalls-Mantey A,Summers MF

    更新日期：2005-03-15 00:00:00

  • Functional characterization of the precursor and spliced forms of RecA protein of Mycobacterium tuberculosis.

    abstract：:The recA locus of pathogenic mycobacteria differs from that of nonpathogenic species because it contains large intervening sequences nested in the RecA homology region that are excised by an unusual protein-splicing reaction. In vivo assays indicated that Mycobacterium tuberculosis recA partially complemented Escheric...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9517751

    authors： Kumar RA,Vaze MB,Chandra NR,Vijayan M,Muniyappa K

    更新日期：1996-02-13 00:00:00

  • Casein kinase II phosphorylation of caldesmon downregulates myosin-caldesmon interactions.

    abstract：:It is well-known that caldesmon (CaD) is a substrate for casein kinase II (CKII), and the phosphorylation of CaD by CKII regulates the interaction of CaD with myosin. However, the functionally relevant CKII phosphorylation site(s) on CaD and the precise role of CaD phosphorylation by CKII in mediating CaD's function h...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0006767

    authors： Wang Z,Yang ZQ

    更新日期：2000-09-12 00:00:00

  • Tritium isotope effects in adenosylcobalamin-dependent methylmalonyl-CoA mutase.

    abstract：:Methylmalonyl-CoA mutase from Propionibacterium shermanii is an adenosylcobalamin-dependent enzyme which catalyzes the reversible isomerization of methylmalonyl-CoA and succinyl-CoA. The rate of tritium loss from 5'-[3H]adenosylcobalamin during the enzymic reaction and the relative rates of tritium appearance in subst...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961250o

    authors： Meier TW,Thomä NH,Leadlay PF

    更新日期：1996-09-10 00:00:00

  • Self base pairing in a complementary deoxydinucleoside monophosphate duplex: crystal and molecular structure of deoxycytidylyl-(3'-5')-deoxyguanosine.

    abstract：:The molecular structure of ammonium deoxycytidylyl-(3'-5')-deoxyguanosine, crystallized from aqueous acetone near pH 4, was determined for X-ray diffraction data. The crystals were tetragonal, space group P43212 with a = b = 11.078 (1) A and c = 45.826 (4) A. The structure was solved by tangent expansion of phases bas...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00277a014

    authors： Cruse WB,Egert E,Kennard O,Sala GB,Salisbury SA,Viswamitra MA

    更新日期：1983-04-12 00:00:00

  • Ultraviolet resonance Raman spectra of cytochrome c conformational states.

    abstract：:Ultraviolet resonance Raman (UV RR) spectra are reported for ferricytochrome c from tuna and horse heart at pH 1.6, 7, 10, and 13, representing distinct conformational states of the protein (states II, III, IV, and V, respectively). The spectra were obtained with pulsed laser excitation at 200 and 218 nm, via H2 Raman...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00339a035

    authors： Copeland RA,Spiro TG

    更新日期：1985-08-27 00:00:00

  • Allosteric interactions of DNA and nucleotides with S. cerevisiae RSC.

    abstract：:RSC (remodel the structure of chromatin) is an essential chromatin remodeler of Saccharomyces cerevisiae that has been shown to have DNA translocase properties. We studied the DNA binding properties of a "trimeric minimal RSC" (RSCt) of the RSC chromatin remodeling complex and the effect of nucleotides on this interac...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200837b

    authors： Malik SS,Rich E,Viswanathan R,Cairns BR,Fischer CJ

    更新日期：2011-09-20 00:00:00

  • The study of pH-dependent stability shows that the TPLH-mediated hydrogen-bonding network is important for the conformation and stability of human gankyrin.

    abstract：:Ankyrin repeat (AR) proteins possess a distinctive modular and repetitive architecture, and their global folds are maintained by short-range interactions in terms of the primary sequence. In this work, we extended our previous study on the highly conserved TPLH tetrapeptide and investigated the impact of a solvent-exp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi4005717

    authors： Yuan C,Guo Y,Zhu L,Guo W,Mahajan A,Weghorst CM,Li J

    更新日期：2013-07-16 00:00:00

  • Proton NMR investigation of substrate-bound heme oxygenase: evidence for electronic and steric contributions to stereoselective heme cleavage.

    abstract：:The substrate-bound form of the enzyme heme oxygenase (HO), which catalyzed the stereospecific alpha-meso bridge cleavage of hemin to yield biliverdin IX alpha, has been investigated by 1H NMR in both its primarily high-spin and its cyanide-inhibited low-spin forms. Both derivatives yield 1H NMR spectra indicative of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00187a033

    authors： Hernández G,Wilks A,Paolesse R,Smith KM,Ortiz de Montellano PR,La Mar GN

    更新日期：1994-05-31 00:00:00

  • Purification and characterization of a transformation-dependent protein secreted by cultured murine fibroblasts.

    abstract：:The major excreted protein (MEP) of transformed mouse fibroblasts has been purified, and monospecific antisera against it have been prepared. Synthesis and secretion of this protein have previously been shown to be stimulated by transformation or treatment with tumor-promoting phorbol esters, but its function is still...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00509a039

    authors： Gottesman MM,Cabral F

    更新日期：1981-03-17 00:00:00

  • RNA phosphorylation: a polynucleotide kinase function in mouse L cell nuclei.

    abstract：:Analysis of [gamma-32P]ATP in vitro labeled nuclear RNA shows transfer of gamma-32P from ATP to form the 5'-terminal monophosphate for large RNA molecules. This finding is an actively transcribing nuclear system capable of guanylation and methylation reactions indicates that polynucleotide kinase activity in the eukar...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00638a016

    authors： Winicov I

    更新日期：1977-09-20 00:00:00

  • Structural composition of canine secretory component and immunoglobulin A.

    abstract：:Dog serum and colostral immunoglobulin A (IgA) and free secretory component from colostrum were isolated using affinity chromatography. Both serum and colostral IgA showed similar susceptibility to reduction with dithiothreitol, but only colostral IgA released the additional subunit, bound secretory component. This re...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00684a010

    authors： Thompson RE,Reynolds HY,Waxdal MJ

    更新日期：1975-07-01 00:00:00

  • Mechanism of flavin reduction in class 2 dihydroorotate dehydrogenases.

    abstract：:Dihydroorotate dehydrogenases (DHODs) oxidize dihydroorotate (DHO) to orotate using the FMN prosthetic group to abstract a hydride equivalent from C6 and a protein residue (Ser for Class 2 DHODs) to deprotonate C5. The fundamental question of whether the scission of the two DHO C-H bonds is concerted or stepwise was a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060919g

    authors： Fagan RL,Nelson MN,Pagano PM,Palfey BA

    更新日期：2006-12-19 00:00:00

  • Integrin-supported fast rate intracellular delivery of plasmid DNA by extracellular matrix protein embedded calcium phosphate complexes.

    abstract：:Extracellular matrix (ECM) proteins, such as collagen and fibronectin, play vital roles in development and maintenance of hard tissue (bone or tooth) and are, consequently, thoroughly investigated for construction of biomimetic scaffolds in combination with calcium phosphate (CaP) material (the major component of hard...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050595g

    authors： Chowdhury EH,Nagaoka M,Ogiwara K,Zohra FT,Kutsuzawa K,Tada S,Kitamura C,Akaike T

    更新日期：2005-09-20 00:00:00

  • DNA binding mechanism of O6-alkylguanine-DNA alkyltransferase: stoichiometry and effects of DNA base composition and secondary structure on complex stability.

    abstract：:O6-Alkylguanine-DNA alkyltransferase (AGT) is an important cellular defense against the mutagenic effects of DNA alkylating agents. In humans this defense can contribute to the ability of some tumors to resist the effects of chemotherapeutic agents that act through DNA alkylation. We report here studies that character...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960971k

    authors： Fried MG,Kanugula S,Bromberg JL,Pegg AE

    更新日期：1996-12-03 00:00:00

  • Conservation of electrostatic properties within enzyme families and superfamilies.

    abstract：:Electrostatic interactions play a key role in enzyme catalytic function. At long range, electrostatics steer the incoming ligand/substrate to the active site, and at short distances, electrostatics provide the specific local interactions for catalysis. In cases in which electrostatics determine enzyme function, orthol...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026918f

    authors： Livesay DR,Jambeck P,Rojnuckarin A,Subramaniam S

    更新日期：2003-04-01 00:00:00

  • NMR studies of DNA support the role of pre-existing minor groove variations in nucleosome indirect readout.

    abstract：:We investigated how the intrinsic sequence-dependent properties probed via the phosphate linkages (BI ↔ BII equilibrium) influence the preferred shape of free DNA, and how this affects the nucleosome formation. First, this exploits NMR solution studies of four B-DNA dodecamers that together cover 39 base pairs of the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500504y

    authors： Xu X,Ben Imeddourene A,Zargarian L,Foloppe N,Mauffret O,Hartmann B

    更新日期：2014-09-09 00:00:00

  • Accumulation of a globo-series glycolipid having Gal alpha 1-3Gal in PC12h pheochromocytoma cells.

    abstract：:In a previous paper, we reported the presence of globoside as a major neutral glycolipid in PC12 pheochromocytoma cells [Ariga, T., Macala, L. J., Saito, M., Margolis, R. K., Greene, L. A., Margolis, R. U., & Yu, R. K. (1988) Biochemistry 27, 52-58]. Recently, we found that subcloned PC12h cells accumulated another un...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00414a058

    authors： Ariga T,Yu RK,Scarsdale JN,Suzuki M,Kuroda Y,Kitagawa H,Miyatake T

    更新日期：1988-07-12 00:00:00

  • Molecular mechanism of spectral tuning in sensory rhodopsin II.

    abstract：:Sensory rhodopsin II (SRII) is unique among the archaeal rhodopsins in having an absorption maximum near 500 nm, blue shifted roughly 70 nm from the other pigments. In addition, SRII displays vibronic structure in the lambda(max) absorption band, whereas the other pigments display fully broadened band maxima. The mole...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0116487

    authors： Ren L,Martin CH,Wise KJ,Gillespie NB,Luecke H,Lanyi JK,Spudich JL,Birge RR

    更新日期：2001-11-20 00:00:00

  • Spectra, membrane binding, and potentiometric responses of new charge shift probes.

    abstract：:The properties of a series of new potentiometric membrane probes have been explored. The probes all contain an (aminostyryl)pyridinium chromophore or a more highly conjugated analogue. The spectral properties of the dyes are discussed in terms of the excitation-induced charge shift from the pyridine to the aniline; th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00342a010

    authors： Fluhler E,Burnham VG,Loew LM

    更新日期：1985-10-08 00:00:00

  • Modulation of GLUT4 and GLUT1 recycling by insulin in rat adipocytes: kinetic analysis based on the involvement of multiple intracellular compartments.

    abstract：:The trafficking kinetics of GLUT4 and GLUT1 in rat epididymal adipocytes were analyzed by a four-compartment model based upon steady-state pool sizes of three intracellular fractions and one plasma membrane fraction separated and assessed under both basal and insulin-stimulated states. The steady-state compartment siz...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0007021

    authors： Lee W,Ryu J,Spangler RA,Jung CY

    更新日期：2000-08-08 00:00:00

  • Structure-function relationships in human glutathione-dependent formaldehyde dehydrogenase. Role of Glu-67 and Arg-368 in the catalytic mechanism.

    abstract：:The active-site zinc in human glutathione-dependent formaldehyde dehydrogenase (FDH) undergoes coenzyme-induced displacement and transient coordination to a highly conserved glutamate residue (Glu-67) during the catalytic cycle. The role of this transient coordination of the active-site zinc to Glu-67 in the FDH catal...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052554q

    authors： Sanghani PC,Davis WI,Zhai L,Robinson H

    更新日期：2006-04-18 00:00:00

  • Rapid plasmenylethanolamine-selective fusion of membrane bilayers catalyzed by an isoform of glyceraldehyde-3-phosphate dehydrogenase: discrimination between glycolytic and fusogenic roles of individual isoforms.

    abstract：:Recently we demonstrated that the unique stereoelectronic relationships inherent in the structure of plasmenylethanolamine facilitate membrane fusion, and we postulated the existence of a membrane fusion protein which could exploit the propensity of plasmenylethanolamine molecular species to adapt an inverted hexagona...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00038a013

    authors： Glaser PE,Gross RW

    更新日期：1995-09-26 00:00:00

  • The stability of chicken nucleoside triphosphate diphosphohydrolase 8 requires both of its transmembrane domains.

    abstract：:Chicken nucleoside triphosphate diphosphohydrolase 8 (NTPDase8) is a cell surface ectonucleotidase with a large extracellular domain (ECD) containing the active site and is anchored to the membrane by two transmembrane domains (TMDs) at the N- and C-termini. Unlike other cell surface NTPDases that have been characteri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901820c

    authors： Li CS,Lee Y,Knowles AF

    更新日期：2010-01-12 00:00:00

  • Observation of multistate kinetics during the slow folding and unfolding of barstar.

    abstract：:The kinetics of the slow folding and unfolding reactions of barstar, a bacterial ribonuclease inhibitor protein, have been studied at 23(+/-1) degrees C, pH 8, by the use of tryptophan fluorescence, far-UV circular dichroism (CD), near-UV CD, and transient mixing (1)H nuclear magnetic resonance (NMR) spectroscopic mea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990285w

    authors： Bhuyan AK,Udgaonkar JB

    更新日期：1999-07-13 00:00:00

  • Tyrosyl interactions in the folding and unfolding of bovine pancreatic ribonuclease A: a study of tyrosine-to-phenylalanine mutants.

    abstract：:Three tyrosine-to-phenylalanine mutants of ribonuclease A (Y25F, Y92F, and Y97F) are investigated for their enzymatic activities, molecular stabilities, and unfolding/refolding kinetics. These mutants exhibit 80, 90, and 80%, respectively, of the catalytic activity of the wild-type enzyme. Thermal, Gdn.HCl, and pH tra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970711i

    authors： Juminaga D,Wedemeyer WJ,Garduño-Júarez R,McDonald MA,Scheraga HA

    更新日期：1997-08-19 00:00:00

  • Electrochemical measurement of second-order electron transfer rate constants for the reaction between cytochrome b5 and cytochrome c.

    abstract：:The second-order electron transfer reaction between cytochrome b5 and cytochrome c has been studied by cyclic voltammetry utilizing a gold electrode modified with beta-mercaptopropionate. When cyclic voltammetry is performed on a solution containing a mixture of cytochrome b5, cytochrome c and polylysine, cytochrome b...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9529723

    authors： Seetharaman R,White SP,Rivera M

    更新日期：1996-09-24 00:00:00

  • Dynamic conformations compared for IgE and IgG1 in solution and bound to receptors.

    abstract：:Dynamic conformations of two distinct immunoglobulin (Ig) isotypes, murine IgE and human IgG1, were examined with fluorescence resonance energy transfer measurements. The IgE mutant epsilon/C gamma 3* and the IgG1 mutant gamma/C gamma 3* each bind [5-(dimethylamino)naphthalen-1-yl]sulfonyl (DNS) in two identical antig...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00148a004

    authors： Zheng Y,Shopes B,Holowka D,Baird B

    更新日期：1992-08-25 00:00:00