Identification of a 33-kilodalton cytoskeletal protein with high affinity for the sodium channel.

abstract：:Protein-protein interactions such as those between small G proteins and their effector proteins control most cell signaling pathways and thereby govern many cellular processes in both normal and disease states. Each small G protein interacts with several effectors, some shared between similar G proteins and others uni...

journal_title：Biochemistry

authors： Elliot-Smith AE,Owen D,Mott HR,Lowe PN

更新日期：2007-12-11 00:00:00

abstract：:A series of plastocyanin mutants have been constructed by site-directed mutagenesis and expressed in Escherichia coli to elucidate the interaction between plastocyanin and photosystem 1 in the photosynthetic electron-transfer chain. Leu-12 has been replaced with alanine, asparagine, glutamate, and lysine, while Tyr-83...

journal_title：Biochemistry

authors： Sigfridsson K,Young S,Hansson O

更新日期：1996-01-30 00:00:00

abstract：:Raman spectra of phycocyanobilin, phycocyanin, and allophycocyanin were obtained at resonance with their visible and near-UV transitions. These spectra were empirically assigned with the help of 14N- and 15N-isotopic substitutions and comparisons with resonance Raman spectra of phycoerythrin. These results confirm the...

journal_title：Biochemistry

authors： Szalontai B,Gombos Z,Csizmadia V,Bagyinka C,Lutz M

更新日期：1994-10-04 00:00:00

abstract：:The trimeric main light-harvesting complex (LHC-II) is the only antenna complex of higher plants of which a high-resolution 3D structure has been obtained (Kühlbrandt, W., Wang, D., and Fujiyoshi, Y. (1994) Nature 367, 614-621) and which can be refolded in vitro from its components. Four different recombinant forms of...

journal_title：Biochemistry

authors： Rogl H,Schödel R,Lokstein H,Kühlbrandt W,Schubert A

更新日期：2002-02-19 00:00:00

abstract：:The prion protein (PrP) is a cell-surface Cu2+ binding glycoprotein which can bind six copper ions. The role of Cu2+ in PrP function, misfolding, and prion disease has generated much interest; however, the field has been hampered by a lack of consensus with regard to the affinity of Cu2+ for PrPC. Here we build on our...

journal_title：Biochemistry

authors： Nadal RC,Davies P,Brown DR,Viles JH

更新日期：2009-09-29 00:00:00

abstract：:The interaction of alamethicin with artificial lecithin multilamellar dispersions was investigated by nuclear magnetic resonance (NMR) and Raman spectroscopies. 31P NMR studies revealed perturbation of the lipid head groups in the presence of the icosapeptide. Simulation of the 31P NMR spectra indicated that the obser...

journal_title：Biochemistry

authors： Banerjee U,Zidovetzki R,Birge RR,Chan SI

更新日期：1985-12-17 00:00:00

abstract：:BamA is an essential component of the β-barrel assembly machine (BAM) that is responsible for insertion and folding of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. BamA is an OMP itself, and its β-barrel transmembrane domain is thought to catalyze OMP insertion and folding, although the molecular...

journal_title：Biochemistry

authors： Doerner PA,Sousa MC

更新日期：2017-06-20 00:00:00

abstract：:Cryptophycin-52 (LY355703) is currently undergoing clinical evaluation for cancer chemotherapy. It is a potent suppressor of microtubule dynamics in vitro, and low picomolar concentrations appear to inhibit cancer cell proliferation at mitosis by stabilizing spindle microtubules. In the present study, using [(3)H]cryp...

journal_title：Biochemistry

authors： Panda D,Ananthnarayan V,Larson G,Shih C,Jordan MA,Wilson L

更新日期：2000-11-21 00:00:00

abstract：:The complete amino acid sequences of two basic proline-rich proteins, IB-1 and IB-6, from human parotid saliva have been determined. Fragments for sequence analysis were obtained by enzymatic digestions. The proteins have molecular weights of 9571 (IB-1) and 11,530 (IB-6) and contain 34 and 39 mol % proline, respectiv...

journal_title：Biochemistry

authors： Kauffman D,Hofmann T,Bennick A,Keller P

更新日期：1986-05-06 00:00:00

abstract：:Helices IV and V in the lactose permease of Escherichia coli contain the major determinants for substrate binding [Glu126 (helix IV), Arg144 (helix V), and Cys148 (helix V)]. Structural and dynamic features of this region were studied by using site-directed sulfhydryl modification of 48 single-Cys replacement mutants ...

journal_title：Biochemistry

authors： Kwaw I,Zen KC,Hu Y,Kaback HR

更新日期：2001-09-04 00:00:00

abstract：:The human transferrin receptor (TfR) has three N-linked oligosaccharides. A combination of site-directed mutagenesis and carbohydrate and protein chemistry was used to characterize the structures of the N-linked oligosaccharides and to map their locations. We find that the type of oligosaccharide at each position was ...

journal_title：Biochemistry

authors： Hayes GR,Williams AM,Lucas JJ,Enns CA

更新日期：1997-04-29 00:00:00

abstract：:The fungal neurotoxin alpha-cyclopiazonic acid (CPA), a nanomolar inhibitor of Ca(2+)-ATPase with a unique pentacyclic indole tetramic acid scaffold, is assembled by a three enzyme pathway CpaS, CpaD, and CpaO in Aspergillus sp. We recently characterized the first pathway-specific enzyme CpaS, a hybrid two module poly...

journal_title：Biochemistry

authors： Liu X,Walsh CT

更新日期：2009-11-24 00:00:00

abstract：:We have previously shown that GTP can replace ATP as an energy source to support vinblastine transport by the multidrug transporter P-glycoprotein (Pgp) in plasma membrane vesicles isolated from the multidrug resistant cell line KB-V1 [Lelong et al. (1992) FEBS Lett. 304, 256-260]. Like [gamma-32P]ATP, [gamma-32P]GTP ...

journal_title：Biochemistry

authors： Lelong IH,Cardarelli CO,Gottesman MM,Pastan I

更新日期：1994-08-02 00:00:00

abstract：:A solubilized preparation of steroid 5 alpha-reductase (EC 1.3.1.30) from rat liver has been used in studies focused toward an understanding of the kinetic mechanism associated with enzyme catalysis. From the results of analyses with product and dead-end inhibitors, a preferentially ordered binding of substrates and r...

journal_title：Biochemistry

authors： Levy MA,Brandt M,Greway AT

更新日期：1990-03-20 00:00:00

abstract：:Periodate-oxidized tRNA(Phe) (tRNA(oxPhe)) behaves as a specific affinity label of tetrameric Escherichia coli phenylalanyl-tRNA synthetase (PheRS). Reaction of the alpha 2 beta 2 enzyme with tRNA(oxPhe) results in the loss of tRNAPhe aminoacylation activity with covalent attachment of 2 mol of tRNA dialdehyde/mol of ...

journal_title：Biochemistry

authors： Hountondji C,Schmitter JM,Beauvallet C,Blanquet S

更新日期：1987-08-25 00:00:00

abstract：:A series of diastereomeric dipeptide amides, containing an N-terminal L-lysyl residue and a C-terminal L- or D-amino acid with a derivatized aromatic ring on the side chain, was synthesized to determine the dependence of (1) the chirality of the N-terminal amino acid alpha-carbon and (2) the length of the N-terminal a...

journal_title：Biochemistry

authors： Sheardy RD,Gabbay EJ

更新日期：1983-04-26 00:00:00

abstract：:The effect of concentrated glycerol on the thermal transitions of chymotrypsinogen and ribonuclease has been examined by differential spectrophotometry at 293 and 287 mm, respectively. It was found that for both proteins addition of glycerol raises the transition temperature, the increase in Tm being greater for ribon...

journal_title：Biochemistry

authors： Gekko K,Timasheff SN

更新日期：1981-08-04 00:00:00

abstract：:Effects of paramagnetic shift reagents on the 13C NMR spectra obtained from single-walled vesicle dispersions of egg phosphatidylcholine enriched with 13C in the N-methyl carbons are investigated. Spectra obtained at 25.1 MHz show that, at Yb3+ to phospholipid molar ratios as low as 0.06, complete resolution of the N-...

journal_title：Biochemistry

authors： Sears B,Hutton WC,Thompson TE

更新日期：1976-04-20 00:00:00

abstract：:The first committed step of guanine nucleotide biosynthesis is the oxidation of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) catalyzed by IMP dehydrogenase. The reaction involves the reduction of NAD(+) with the formation of a covalent enzyme intermediate (E-XMP). Hydrolysis of E-XMP requires th...

journal_title：Biochemistry

authors： Guillén Schlippe YV,Hedstrom L

更新日期：2005-09-06 00:00:00

abstract：:In the HLH-LZ protein family, the helix-loop-helix DNA-binding dimerization domain is followed in the sequence by a leucine zipper motif. The precise function of this second dimerization domain is still unclear, since the HLH motif of a subset of this family has been shown to be necessary and sufficient for dimerizati...

journal_title：Biochemistry

authors： Muhle-Goll C,Gibson T,Schuck P,Schubert D,Nalis D,Nilges M,Pastore A

更新日期：1994-09-20 00:00:00

abstract：:The respiratory nitric oxide reductase (NOR) from Paracoccus denitrificans catalyzes the two-electron reduction of NO to N(2)O (2NO + 2H(+) + 2e(-) --> N(2)O + H(2)O), which is an obligatory step in the sequential reduction of nitrate to dinitrogen known as denitrification. NOR has four redox-active cofactors, namely,...

journal_title：Biochemistry

authors： Flock U,Watmough NJ,Adelroth P

更新日期：2005-08-09 00:00:00

abstract：:Sequence-specific assignments of 1H NMR resonances are obtained for the backbone protons of Escherichia coli acyl carrier protein, acylated with an eight-carbon chain covalently attached to the prosthetic group thiol (octanoyl-ACP). Comparison of 1H-1H sequential connectivities in the NOESY spectra of octanoyl-ACP and...

journal_title：Biochemistry

authors： Jones PJ,Holak TA,Prestegard JH

更新日期：1987-06-16 00:00:00

abstract：:A panel of six naïve 14-residue random peptide libraries displayed polyvalently on M13 phage was pooled and sorted against human leukemia inhibitory factor (LIF). After four rounds of selection, a single large family of peptides with the consensus sequence XCXXXXG(A/S)(D/E)(W/F)WXCF was found to bind specifically to L...

journal_title：Biochemistry

authors： Fairlie WD,Uboldi AD,Hemmings GJ,Smith BJ,Martin HM,Morgan PO,Baca M

更新日期：2003-11-18 00:00:00

abstract：:The mechanism of activation of cGMP phosphodiesterase by the GTP-binding protein in the disc membrane of retinal rods has been investigated by measuring the light-induced phosphodiesterase activity in reconstituted systems where the concentration of either the GTP-binding protein or the phosphodiesterase is varied. Th...

journal_title：Biochemistry

authors： Bennett N,Clerc A

更新日期：1989-09-05 00:00:00

abstract：:The reaction of mushroom (Agaricus bisporus) tyrosinase with dioxygen in the presence of several o-diphenolic substrates has been studied by steady-state and transient-phase kinetics in order to elucidate the rate-limiting step and to provide new insights into the mechanism of oxidation of these substrates. A kinetic ...

journal_title：Biochemistry

authors： Rodríguez-López JN,Fenoll LG,García-Ruiz PA,Varón R,Tudela J,Thorneley RN,García-Cánovas F

更新日期：2000-08-29 00:00:00

abstract：:Understanding the evolution of allostery in multidomain enzymes remains an important step in improving our ability to identify and exploit structure-function relationships in allosteric mechanisms. A recent protein similarity network for the DRE-TIM metallolyase superfamily indicated there are two evolutionarily disti...

journal_title：Biochemistry

authors： Kumar G,Frantom PA

更新日期：2014-07-29 00:00:00

abstract：:The C-terminal src kinase (CSK) is a ubiquitously expressed, cytosolic enzyme capable of phosphorylating and inactivating several plasma membrane-bound src-family protein tyrosine kinases in vitro [Nada, S., Okada, M., MacAuley, A., Cooper, J.A., & Nakagawa, H. (1990) Nature 351, 69-72; Bergman, M., Mustelin, T., Oetk...

journal_title：Biochemistry

authors： Cheng HC,Bjorge JD,Aebersold R,Fujita DJ,Wang JH

更新日期：1996-09-10 00:00:00

abstract：:Phoborhodopsin from Halobacterium salinarum (salinarum phoborhodopsin, spR also called HsSR II) is a photoreceptor for the negative phototaxis of the bacterium. A unique feature of spR is the formation of a shorter wavelength photoproduct, P480, observed at liquid nitrogen temperature beside the K intermediate. Format...

journal_title：Biochemistry

authors： Dai G,Zhang Y,Tamogami J,Demura M,Kamo N,Kandori H,Iwasa T

更新日期：2011-08-23 00:00:00

abstract：:The interaction of nile red (NR) with apomyoglobin (ApoMb) in the native (pH 7) and molten globule (pH 4) states was investigated using experimental and computational methods. NR binds to hydrophobic locations in ApoMb with higher affinity (K(d) = 25 +/- 5 microM) in the native state than in the molten globule state (...

journal_title：Biochemistry

authors： Polverini E,Cugini G,Annoni F,Abbruzzetti S,Viappiani C,Gensch T

更新日期：2006-04-25 00:00:00

abstract：:The late intermediates involved in the activation mechanism of bovine rhodopsin are investigated by time-resolved optical absorption spectroscopy. Measurements from 10 μs to 200 ms after photolysis were carried out on membrane suspensions of bovine rhodopsin at a temperature of 15 °C and at pH of 7.3, 8.0, and 8.7. Th...

journal_title：Biochemistry

authors： Szundi I,Funatogawa C,Kliger DS

更新日期：2016-09-13 00:00:00