Abstract：
:Cell free protein synthesis (CFPS) has emerged as a promising methodology for protein expression. While polypeptide production is very reliable and efficient using CFPS, the correct cotranslational folding of membrane proteins during CFPS is still a challenge. In this contribution, we describe a two-step protocol in which the integral membrane protein is initially expressed by CFPS as a precipitate followed by an in vitro folding procedure using lipid vesicles for converting the protein precipitate to the correctly folded protein. We demonstrate the feasibility of using this approach for the K(+) channels KcsA and MVP and the amino acid transporter LeuT. We determine the crystal structure of the KcsA channel obtained by CFPS and in vitro folding to show the structural similarity to the cellular expressed KcsA channel and to establish the feasibility of using this two-step approach for membrane protein production for structural studies. Our studies show that the correct folding of these membrane proteins with complex topologies can take place in vitro without the involvement of the cellular machinery for membrane protein biogenesis. This indicates that the folding instructions for these complex membrane proteins are contained entirely within the protein sequence.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Focke PJ,Hein C,Hoffmann B,Matulef K,Bernhard F,Dötsch V,Valiyaveetil FIdoi
10.1021/acs.biochem.6b00488subject
Has Abstractpub_date
2016-08-02 00:00:00pages
4212-9issue
30eissn
0006-2960issn
1520-4995journal_volume
55pub_type
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