Active site specific cadmium(II)-substituted horse liver alcohol dehydrogenase: crystal structures of the free enzyme, its binary complex with NADH, and the ternary complex with NADH and bound p-bromobenzyl alcohol.

Abstract:

:Three crystal structures have been determined of active site specific substituted Cd(II) horse liver alcohol dehydrogenase and its complexes. Intensities were collected for the free, orthorhombic enzyme to 2.4-A resolution and for a triclinic binary complex with NADH to 2.7-A resolution. A ternary complex was crystallized from an equilibrium mixture of NAD+ and p-bromobenzyl alcohol. The microspectrophotometric analysis of these single crystals showed the protein-bound coenzyme to be largely NADH, which proves the complex to consist of CdII-LADH, NADH, and p-bromobenzyl alcohol. Intensity data for this abortive ternary complex were collected to 2.9-A resolution. The coordination geometry in the free Cd(II)-substituted enzyme is highly similar to that of the native enzyme. Cd(II) is bound to Cys-46, Cys-174, His-67, and a water molecule in a distorted tetrahedral geometry. Binding of coenzymes induces a conformational change similar to that in the native enzyme. The interactions between the coenzyme and the protein in the binary and ternary complexes are highly similar to those in the native ternary complexes. The substrate binds directly to the cadmium ion in a distorted tetrahedral geometry. No large, significant structural changes compared to the native ternary complex with coenzyme and p-bromobenzyl alcohol were found. The implications of these results for the use of active site specific Cd(II)-substituted horse liver alcohol dehydrogenase as a model system for the native enzyme are discussed.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Schneider G,Cedergren-Zeppezauer E,Knight S,Eklund H,Zeppezauer M

doi

10.1021/bi00346a070

subject

Has Abstract

pub_date

1985-12-03 00:00:00

pages

7503-10

issue

25

eissn

0006-2960

issn

1520-4995

journal_volume

24

pub_type

杂志文章
  • The gene for human protein Z is localized to chromosome 13 at band q34 and is coded by eight regular exons and one alternative exon.

    abstract::Human protein Z is a vitamin K-dependent plasma glycoprotein, deficiency of which leads to a mild bleeding tendency. Protein Z appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. In this study, to characterize the gene for protein Z, its organization and structure...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi972002a

    authors: Fujimaki K,Yamazaki T,Taniwaki M,Ichinose A

    更新日期:1998-05-12 00:00:00

  • Inhibition of CMP-sialic acid transport into Golgi vesicles by nucleoside monophosphates.

    abstract::We examined the interactions of nucleotides with the CMP-sialic acid transporter in order to better understand which features play a role in binding and to investigate the relationship between binding and subsequent transport. With respect to the sugar, the transporter requires a complete ribose ring for tight binding...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi011262w

    authors: Chiaramonte M,Koviach JL,Moore C,Iyer VV,Wagner CR,Halcomb RL,Miller W,Melançon P,Kuchta RD

    更新日期:2001-11-27 00:00:00

  • Unusual Ca(2+)-calmodulin binding interactions of the microtubule-associated protein F-STOP.

    abstract::F-STOP is a microtubule-associated protein that stabilizes microtubules in a calmodulin (CaM)-dependent manner. All members of the stable tubule only polypeptide (STOP) family have a central domain that contains nearly identical multiple repeats, and a CaM binding motif is present in multiple copies within this domain...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi034746w

    authors: Bouvier D,Vanhaverbeke C,Simorre JP,Arlaud GJ,Bally I,Forge V,Margolis RL,Gans P,Kleman JP

    更新日期:2003-10-07 00:00:00

  • Isotope effect studies of the pyridoxal 5'-phosphate dependent histidine decarboxylase from Morganella morganii.

    abstract::The pyridoxal 5'-phosphate dependent histidine decarboxylase from Morganella morganii shows a nitrogen isotope effect k14/k15 = 0.9770 +/- 0.0021, a carbon isotope effect k12/k13 = 1.0308 +/- 0.0006, and a carbon isotope effect for L-[alpha-2H]histidine of 1.0333 +/- 0.0001 at pH 6.3, 37 degrees C. These results indic...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00416a016

    authors: Abell LM,O'Leary MH

    更新日期:1988-08-09 00:00:00

  • Monoclonal antibody to microtubule-associated STOP protein: affinity purification of neuronal STOP activity and comparison of antigen with activity in neuronal and nonneuronal cell extracts.

    abstract::Microtubules, ordinarily cold-labile structures, are made entirely resistant to cold temperature by the presence of substoichiometric amounts of STOP (stable tubule only polypeptide), a microtubule-associated protein. We have produced a monoclonal antibody which specifically recognizes a 145-kDa protein previously imp...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00428a064

    authors: Pirollet F,Rauch CT,Job D,Margolis RL

    更新日期:1989-01-24 00:00:00

  • Structure and function of PA4872 from Pseudomonas aeruginosa, a novel class of oxaloacetate decarboxylase from the PEP mutase/isocitrate lyase superfamily.

    abstract::Pseudomonas aeruginosa PA4872 was identified by sequence analysis as a structurally and functionally novel member of the PEP mutase/isocitrate lyase superfamily and therefore targeted for investigation. Substrate screens ruled out overlap with known catalytic functions of superfamily members. The crystal structure of ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi701954p

    authors: Narayanan BC,Niu W,Han Y,Zou J,Mariano PS,Dunaway-Mariano D,Herzberg O

    更新日期:2008-01-08 00:00:00

  • A phosphotyrosyl mimetic peptide reverses impairment of insulin-stimulated translocation of GLUT4 caused by overexpression of PTP1B in rat adipose cells.

    abstract::Biological actions of insulin are initiated by activation of the insulin receptor tyrosine kinase. Protein tyrosine phosphatases (PTPases) PTP1B and PTPalpha are known to dephosphorylate the insulin receptor and may contribute to insulin resistance in diseases such as diabetes. We previously reported that overexpressi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9816103

    authors: Chen H,Cong LN,Li Y,Yao ZJ,Wu L,Zhang ZY,Burke TR Jr,Quon MJ

    更新日期:1999-01-05 00:00:00

  • Low permeability of liposomal membranes composed of bipolar tetraether lipids from thermoacidophilic archaebacterium Sulfolobus acidocaldarius.

    abstract::The physical origin of the extremely high thermal stability of tetraether liposomes composed of the polar lipid fraction E (PLFE) from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius has been investigated. The leakage rate of trapped 5, 6-carboxyfluorescein (5(6)CF) and the proton permeability in PLFE ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi972163e

    authors: Komatsu H,Chong PL

    更新日期:1998-01-06 00:00:00

  • The binding of complementary oligoribonucleotides to yeast initiator Transfer RNA.

    abstract::Oligoribonucleotide binding to baker's yeast initiator tRNA was measured by equilibrium dialysis in order to determine which regions of the tRNA were free to bind complementary oligomers and which were involved in secondary and tertiary structure. Association constants of trinucleoside diphosphates and tetranucleoside...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00686a004

    authors: Freier SM,Tinoco I Jr

    更新日期:1975-07-29 00:00:00

  • Effect of thioredoxin deletion and p53 cysteine replacement on human p53 activity in wild-type and thioredoxin reductase null yeast.

    abstract::Reporter gene transactivation by human p53 is inhibited in budding yeast lacking the TRR1 gene encoding thioredoxin reductase. To investigate the role of thioredoxin in controlling p53 activity, the level of reporter gene transactivation by p53 was determined in yeast lacking the TRX1 and TRX2 genes encoding cytosolic...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi900757q

    authors: Stoner CS,Pearson GD,Koç A,Merwin JR,Lopez NI,Merrill GF

    更新日期:2009-09-29 00:00:00

  • Investigating the in Vitro Thermal Stability and Conformational Flexibility of Estrogen Receptors as Potential Key Factors of Their in Vivo Activity.

    abstract::Among hormone-inducible transcription factors, estrogen receptors (ERs) play important roles in tissue growth and differentiation, via either direct or indirect binding, in the nucleus, to specific DNA targets called estrogen responsive elements (EREs), or through nongenomic pathways. In humans, two estrogen receptor ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.5b00026

    authors: Le Grand A,André-Leroux G,Marteil G,Duval H,Sire O,Le Tilly V

    更新日期:2015-06-30 00:00:00

  • Identification of tyrosine residues on ELMO1 that are phosphorylated by the Src-family kinase Hck.

    abstract::The SH3 and SH2 domains of hematopoietic cell kinase (Hck) play important roles in substrate targeting. To identify new components of Hck signaling pathways, we identified proteins that bind to the SH3 domain of Hck (Scott et al. (2002) J. Biol. Chem. 277, 28238). One such protein was ELMO1, the mammalian orthologue o...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0500832

    authors: Yokoyama N,deBakker CD,Zappacosta F,Huddleston MJ,Annan RS,Ravichandran KS,Miller WT

    更新日期:2005-06-21 00:00:00

  • Photooxidation pathway of chlorophyll Z in photosystem II as Studied by Fourier transform infrared spectroscopy.

    abstract::The oxidation pathway of chlorophyll Z (ChlZ) in photosystem II (PSII) at cryogenic temperatures was studied by means of light-induced Fourier transform infrared (FTIR) difference spectroscopy. To examine the involvement of redox-active beta-carotene (Car) in the pathway, two Car molecules in Mn-depleted PSII membrane...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi052346y

    authors: Kitajima Y,Noguchi T

    更新日期:2006-02-14 00:00:00

  • Kinetic and structural analysis of enzyme sliding on a substrate: multiple attack in beta-amylase.

    abstract::Beta-amylase (EC 3.2.1.2) is starch-hydrolyzing exo-type enzyme that can catalyze the successive liberation of beta-maltose from the nonreducing ends of alpha-1,4-linked glucopyranosyl polymers. There is a well-known phenomenon called multiple or repetitive attack where the enzyme releases several maltose molecules in...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi061605w

    authors: Ishikawa K,Nakatani H,Katsuya Y,Fukazawa C

    更新日期:2007-01-23 00:00:00

  • Affinity cleavage at the metal-binding site of phosphoenolpyruvate carboxykinase.

    abstract::Chicken liver phosphoenolpyruvate carboxykinase (PEPCK) was rapidly inactivated by micromolar concentrations of ferrous sulfate in the presence of ascorbate at pH 7.4. Omitting ascorbate or replacing the Fe2+ with Mn2+ or Mg2+ gives no inactivation. Mn2+, Mg2+, or Co2+ at 100-fold molar excess over Fe2+ offered comple...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi970574p

    authors: Hlavaty JJ,Nowak T

    更新日期:1997-12-09 00:00:00

  • Sulfonium Ion Condensation: The Burden Borne by SAM Synthetase.

    abstract::S-Adenosylmethionine (SAM+) serves as the principal methylating agent in biological systems, but the thermodynamic basis of its reactivity does not seem to have been clearly established. Here, we show that methionine, methanol, and H+ combine to form S-methylmethionine (SMM+) with a temperature-independent equilibrium...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.8b00477

    authors: Lewis CA Jr,Wolfenden R

    更新日期:2018-07-03 00:00:00

  • Fluorescence investigation of the sex steroid binding protein of rabbit serum: steroid binding and subunit dissociation.

    abstract::The relationship between steroid binding and protein subunit interactions of rabbit sex steroid binding protein (rSBP) has been studied by steady-state and time-resolved fluorescence spectroscopy. The high-affinity (Ka approximately 10(8) M-1 at 4 degrees C), fluorescent estrogen d-1,3,5(10),6,8-estrapentaene-3,17 bet...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00492a005

    authors: Casali E,Petra PH,Ross JB

    更新日期:1990-10-09 00:00:00

  • S-(4-Bromo-2,3-dioxobutyl)glutathione: a new affinity label for the 4-4 isoenzyme of rat liver glutathione S-transferase.

    abstract::S-(4-Bromo-2,3-dioxobutyl)glutathione (S-BDB-G), a reactive analogue of glutathione, has been synthesized and characterized by UV spectroscopy and thin-layer chromatography, as well as by bromide and primary amine analysis. Incubation of S-BDB-G (200 microM) with the 4-4 isoenzyme of rat liver glutathione S-transferas...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00111a006

    authors: Katusz RM,Colman RF

    更新日期:1991-11-26 00:00:00

  • Fluorescence energy transfer measurements of spatial relationships between sulfhydryl groups of thiolase I from porcine heart.

    abstract::Mitochondrial thiolase I from pig heart has been found to have at least two and possibly three reactive sulfhydryl residues at or near the active site [Izbicka-Dimitrijević, E., & Gilbert, H. F. (1982) Biochemistry 21, 6112-6118; Izbicka-Dimitrijević, E., & Gilbert, H. F. (1984) Biochemistry 23, 4318-4324]. In the nat...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00321a015

    authors: Izbicka E,Gilbert HF

    更新日期:1984-12-18 00:00:00

  • Methylated pyrimidines stabilize an alternating conformation of poly(dA-dU).poly(dA-dU).

    abstract::We have investigated the effect of increasing percentages of methylated pyrimidines on the structure of poly(dA-dU).poly(dA-dU). This was done by synthesizing analogous polynucleotides that contained deoxythymidine residues as well as deoxyuridine residues and observing their 31P NMR spectra in increasing amounts of C...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00341a033

    authors: Wu HY,Behe MJ

    更新日期:1985-09-24 00:00:00

  • A putative Fe2+-bound persulfenate intermediate in cysteine dioxygenase.

    abstract::The common reactions of dioxygen, superoxide, and hydroperoxides with thiolates are thought to proceed via persulfenate intermediates, yet these have never been visualized. Here we report a 1.4 A resolution crystal structure of the Fe(2+)-dependent enzyme cysteine dioxygenase (CDO) containing this putative intermediat...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi801546n

    authors: Simmons CR,Krishnamoorthy K,Granett SL,Schuller DJ,Dominy JE Jr,Begley TP,Stipanuk MH,Karplus PA

    更新日期:2008-11-04 00:00:00

  • Interaction of Cibacron Blue F3GA with glutamine synthetase: use of the dye as a conformational probe. 1. Studies using unfractionated dye samples.

    abstract::Cibacron Blue F3GA dye has been used to probe subtle conformational changes in protein structure associated with the conversion of Escherichia coli glutamine synthetase (GS) between relaxed, taut, oxidized, and dissociated forms. Binding of the dye to each form of the enzyme elicits a different spectral perturbation o...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00324a017

    authors: Federici MM,Chock PB,Stadtman ER

    更新日期:1985-01-29 00:00:00

  • Light inhibition of bovine retinal rod guanylyl cyclase mediated by beta gamma-transducin.

    abstract::Photoreceptor guanylyl cyclase (ROS-GC), converting GTP into cGMP and pyrophosphate, is a key enzyme in the regulation of the visual transduction cascade. ROS-GC requires GC-activating proteins (GCAPs) and low free [Ca] for full activity. We found that when choline or potassium were the major cations present, light ca...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9825596

    authors: Wolbring G,Baehr W,Palczewski K,Schnetkamp PP

    更新日期:1999-03-02 00:00:00

  • The metastasis-promoting phosphatase PRL-3 shows activity toward phosphoinositides.

    abstract::Phosphatase of regenerating liver 3 (PRL-3) is suggested as a biomarker and therapeutic target in several cancers. It has a well-established causative role in cancer metastasis. However, little is known about its natural substrates, pathways, and biological functions, and only a few protein substrates have been sugges...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi201095z

    authors: McParland V,Varsano G,Li X,Thornton J,Baby J,Aravind A,Meyer C,Pavic K,Rios P,Köhn M

    更新日期:2011-09-06 00:00:00

  • Molecular cloning and developmental expression of the caveolin gene family in the amphibian Xenopus laevis.

    abstract::Caveolae are approximately 50-100 nm invaginations of the plasma membrane thought to form as a result of a local accumulation of cholesterol, sphingolipids, and a unique family of three proteins known as the caveolins: Cav-1, -2, and -3. Here, we report the identification, sequence, and developmental expression of the...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi020043n

    authors: Razani B,Park DS,Miyanaga Y,Ghatpande A,Cohen J,Wang XB,Scherer PE,Evans T,Lisanti MP

    更新日期:2002-06-25 00:00:00

  • Syntheses of biotinylated and dethiobiotinylated insulins.

    abstract::The 600-MHz proton spectrum of dethiobiotin (prepared from d-biotin with Raney nickel) was measured in order to gain information pertaining to its stereochemical homogeneity. The spectrum demonstrated clearly that the material is a 6:1 mixture of two stereoisomers. The cis compound, corresponding to the stereochemistr...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00307a002

    authors: Hofmann K,Zhang WJ,Romovacek H,Finn FM,Bothner-By AA,Mishra PK

    更新日期:1984-06-05 00:00:00

  • Specific stimulation of the T7 gene 6 exonuclease by the phage T7 coded deoxyribonucleic acid binding protein.

    abstract::Bacteriophage T7 codes for a single-stranded DNA binding protein. This protein is the product of gene 2.5 and has been found previously to stimulate specifically the activity of the phage-coded DNA polymerase. We report here that the T7 DNA binding protein also stimulates the activity of the phage-coded exonuclease. T...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00266a042

    authors: Roberts L,Sadowski P,Wong JT

    更新日期:1982-11-09 00:00:00

  • Formation of highly ordered multimers in G-quadruplexes.

    abstract::G-Rich DNA and RNA have a higher propensity to form G-quadruplex structures, but the presence of G-runs alone is not sufficient to prove that such sequences can form stable G-quadruplexes. While G-rich sequences are essential for G-quadruplex formation, not all G-rich sequences have the propensity to form G-quadruplex...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi500773c

    authors: Tóthová P,Krafčíková P,Víglaský V

    更新日期:2014-11-18 00:00:00

  • The HIV-1 gp41 N-terminal heptad repeat plays an essential role in membrane fusion.

    abstract::For many different enveloped viruses the crystal structure of the fusion protein core has been established. A striking conservation in the tertiary and quaternary arrangement of these core structures is repeatedly revealed among members of diverse families. It has been proposed that the primary role of the core involv...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0255322

    authors: Sackett K,Shai Y

    更新日期:2002-04-09 00:00:00

  • Nucleotide sequence of the promoter and fadB gene of the fadBA operon and primary structure of the multifunctional fatty acid oxidation protein from Escherichia coli.

    abstract::The primary structure of a multifunctional protein, the large alpha-subunit of the Escherichia coli fatty acid oxidation complex, was determined by sequencing the fadB region of the fadBA operon. The amino-terminal sequence of this protein had been established by Edman degradation. The transcription start site of the ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00241a023

    authors: Yang XY,Schulz H,Elzinga M,Yang SY

    更新日期:1991-07-09 00:00:00