Three-state denaturation of DnaK induced by guanidine hydrochloride. Evidence for an expandable intermediate.

abstract：:alpha5beta1 integrin can occupy several distinct conformational states which support different strengths of binding to fibronectin [García, A. J., et al. (1998) J. Biol. Chem. 273, 34710-34715]. Using a model system in which specific activating monoclonal antibodies were used to achieve uniform activated states, the b...

journal_title：Biochemistry

authors： García AJ,Schwarzbauer JE,Boettiger D

更新日期：2002-07-23 00:00:00

abstract：:The electron spin resonance probes 5-doxylstearate and 4-(dodecyldimethylammonio)-1-oxy-2,2,6,6-tetramethylpiperidine bromide were used to characterize the fluidity of the acyl chain and head-group regions, respectively, of defined salts of lipopolysaccharide (LPS) from Escherichia coli K12. The removal of the weakly ...

journal_title：Biochemistry

authors： Coughlin RT,Haug A,McGroarty EJ

更新日期：1983-04-12 00:00:00

abstract：:Heparin decreases the rate of inhibition of neutrophil elastase by alpha 1-proteinase inhibitor as a result of its strong binding to the enzyme. Here, we used the slow-binding kinetic approach to decide whether the enzyme-inhibitor interaction proceeds via a two-step mechanism and to identify the step that is affected...

journal_title：Biochemistry

authors： Faller B,Cadène M,Bieth JG

更新日期：1993-09-07 00:00:00

abstract：:Aromatic amino acids often flank the transmembrane alpha helices of integral membrane proteins. By favoring locations within the membrane-water interface of the lipid bilayer, aromatic residues Trp, Tyr, and sometimes Phe may serve as anchors to help stabilize a transmembrane orientation. In this work, we compare the ...

journal_title：Biochemistry

authors： Sparks KA,Gleason NJ,Gist R,Langston R,Greathouse DV,Koeppe RE 2nd

更新日期：2014-06-10 00:00:00

abstract：:To determine the energetic and structural consequences of placing a charged group within the core of a protein, two "buried charge" mutants, Met 102----Lys (M102K) and Leu 133----Asp (L133D) were constructed in phage T4 lysozyme. Both proteins fold at neutral pH, although they are substantially less stable than wild t...

journal_title：Biochemistry

authors： Dao-pin S,Anderson DE,Baase WA,Dahlquist FW,Matthews BW

更新日期：1991-12-10 00:00:00

abstract：:Structurally diverse small molecules, including 5-(2-benzofuryl)-4-phenyl-1,2,4-triazole-3-thiol (BETT), have been identified via high-throughput screening as activators of caspase-3 in HeLa cell extracts. However, little is known about their mechanism of action. In this study, we investigate how BETT regulates prothy...

journal_title：Biochemistry

authors： Qi X,Wang L,Du F

更新日期：2010-03-09 00:00:00

abstract：:Melittin is a 26-residue peptide which undergoes high-affinity calcium-dependent binding by calmodulin [Barnette, M.S., Daly, R., & Weiss, B. (1983) Biochem. Pharmacol. 32, 2929; Comte, M., Maulet, Y., & Cox, J.A. (1983) Biochem. J. 209, 269; Anderson, S.R., & Malencik, D.A. (1986) Calcium Cell Funct. 6, 1]. The resul...

journal_title：Biochemistry

authors： Malencik DA,Anderson SR

更新日期：1988-03-22 00:00:00

abstract：:One of the distinctive features of eubacterial retinal-based proton pumps, proteorhodopsins, xanthorhodopsin, and others, is hydrogen bonding of the key aspartate residue, the counterion to the retinal Schiff base, to a histidine. We describe properties of the recently found eubacterium proton pump from Exiguobacteriu...

journal_title：Biochemistry

authors： Balashov SP,Petrovskaya LE,Lukashev EP,Imasheva ES,Dioumaev AK,Wang JM,Sychev SV,Dolgikh DA,Rubin AB,Kirpichnikov MP,Lanyi JK

更新日期：2012-07-24 00:00:00

abstract：:Locusta migratoria apolipophorin III (apoLp-III) is a helix bundle exchangeable apolipoprotein that reversibly binds to lipoprotein surfaces. Structural reorganization of its five amphipathic alpha-helices enables the transition from the lipid-free to lipid-bound state. ApoLp-III-induced transformation of dimyristoylp...

journal_title：Biochemistry

authors： Weers PM,Kay CM,Ryan RO

更新日期：2001-06-26 00:00:00

abstract：:The organometallic anticancer agent titanocene dichloride, Cp(2)TiCl(2), is now in phase II clinical trials as an anticancer drug, but its mechanism of action is poorly understood. We show here that the interactions of Cp(2)TiCl(2) with human serum transferrin (hTF) and that of Ti(2)-hTF with adenosine triphosphate (A...

journal_title：Biochemistry

authors： Guo M,Sun H,McArdle HJ,Gambling L,Sadler PJ

更新日期：2000-08-22 00:00:00

abstract：:Bacteriophage lambda protein phosphatase (lambdaPP) is a member of a large family of metal-containing phosphoesterases, including purple acid phosphatase, protein serine/threonine phosphatases, 5'-nucleotidase, and DNA repair enzymes such as Mre11. lambdaPP can be activated several-fold by various divalent metal ions,...

journal_title：Biochemistry

authors： Reiter TA,Reiter NJ,Rusnak F

更新日期：2002-12-24 00:00:00

abstract：:In all structurally characterized bacterial multicomponent monooxygenase (BMM) hydroxylase proteins, a series of hydrophobic cavities in the α-subunit trace a conserved path from the protein exterior to the carboxylate-bridged diiron active site. This study examines these cavities as a potential route for transport of...

journal_title：Biochemistry

authors： McCormick MS,Lippard SJ

更新日期：2011-12-27 00:00:00

abstract：:The complete amino acid sequences of two basic proline-rich proteins, IB-1 and IB-6, from human parotid saliva have been determined. Fragments for sequence analysis were obtained by enzymatic digestions. The proteins have molecular weights of 9571 (IB-1) and 11,530 (IB-6) and contain 34 and 39 mol % proline, respectiv...

journal_title：Biochemistry

authors： Kauffman D,Hofmann T,Bennick A,Keller P

更新日期：1986-05-06 00:00:00

abstract：:Because of the lack of protein turnover in fiber cells of the ocular lens, Aquaporin 0 (AQP0), the most abundant membrane protein in the lens, undergoes extensive post-translational modification with fiber cell age. To map the distribution of modified forms of AQP0 within the lens, normal human lenses ranging in age f...

journal_title：Biochemistry

authors： Ball LE,Garland DL,Crouch RK,Schey KL

更新日期：2004-08-03 00:00:00

abstract：:To study the role of the N-terminal region of the estrogen receptor (ER) in transcription activation and in DNA binding, we constructed a mutant of the Xenopus laevis ER which lacks amino acids 1-159 (XER160/586). In transient transfections, XER160/586 exhibited < 10% of the activity of wild-type XER on a synthetic pr...

journal_title：Biochemistry

authors： Xing H,Mattick S,Lew D,Shapiro DJ

更新日期：1995-03-28 00:00:00

abstract：:The mechanisms for substrate recognition by two cytoplasmic protein tyrosine phosphatases, PTP-5 and rrbPTP-1, were investigated. Phosphorylation sites on tyrosine-phosphorylated casein, a model PTP substrate, were characterized. Two peptides based on casein phosphorylation sites and one peptide based on the tyrosine ...

journal_title：Biochemistry

authors： Hippen KL,Jakes S,Richards J,Jena BP,Beck BL,Tabatabai LB,Ingebritsen TS

更新日期：1993-11-23 00:00:00

abstract：:The time-resolved fluorescence decay of plant PSI-LHCI has been analyzed and compared with its component parts, the PSI core and the peripheral antenna LHCI, in an attempt to (i) define the physical domains associated with the multicomponent decay-associated spectra (DAS) and determine the origin of the kinetically sl...

journal_title：Biochemistry

authors： Engelmann E,Zucchelli G,Casazza AP,Brogioli D,Garlaschi FM,Jennings RC

更新日期：2006-06-06 00:00:00

abstract：:A human genomic DNA library was screened for the gene coding for the gamma chain of fibrinogen by using a human cDNA for the gamma chain as a hybridization probe. The gene was identified in three overlapping recombinant lambda bacteriophage, and its sequence, including the immediate 5' and 3' flanking regions, was det...

journal_title：Biochemistry

authors： Rixon MW,Chung DW,Davie EW

更新日期：1985-04-09 00:00:00

abstract：:To study whether protein kinase C (PKC) isoforms can interact with protein-tyrosine-phosphatases (PTPs) which are connected to the insulin signaling pathway, we co-overexpressed PKC isoforms together with insulin receptor, docking proteins, and the PTPs SHP1 and SHP2 in human embryonic kidney (HEK) 293 cells. After ph...

journal_title：Biochemistry

authors： Strack V,Krützfeldt J,Kellerer M,Ullrich A,Lammers R,Häring HU

更新日期：2002-01-15 00:00:00

abstract：:We have investigated the effect of increasing percentages of methylated pyrimidines on the structure of poly(dA-dU).poly(dA-dU). This was done by synthesizing analogous polynucleotides that contained deoxythymidine residues as well as deoxyuridine residues and observing their 31P NMR spectra in increasing amounts of C...

journal_title：Biochemistry

authors： Wu HY,Behe MJ

更新日期：1985-09-24 00:00:00

abstract：:The sensitivity of the fluorescence energy of 2-p-toluidinylnaphthalene-6-sulfonate (TNS) to apparent solvent polarity is considerably greater in methanol-water mixtures and lower primary alcohols than in higher primary alcohols or solvents containing no hydroxyl groups. It is suggested that the behavior of TNS in low...

journal_title：Biochemistry

authors： Greene FC

更新日期：1975-02-25 00:00:00

abstract：:Serotonergic signaling appears to play a key role in the generation and modulation of various cognitive, behavioral, and developmental processes. The serotonin(1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors and is the most extensively studied among the ...

journal_title：Biochemistry

authors： Pucadyil TJ,Kalipatnapu S,Harikumar KG,Rangaraj N,Karnik SS,Chattopadhyay A

更新日期：2004-12-21 00:00:00

abstract：:We have previously suggested that folding of the SV40 attenuator RNA 1 into two hairpin elements leads to a block of transcription elongation. Using site-directed mutagenesis, we created three templates that either strengthened or weakened the proposed hairpin structures. The mutated and wild-type templates were clone...

journal_title：Biochemistry

authors： Goldring NB,Kessler M,Aloni Y

更新日期：1992-09-08 00:00:00

abstract：:The argument and experimental evidence are presented for a two-state model that explains the action of both helical and beta-sheet antimicrobial peptides after they bind to the plasma membranes of cells. Each peptide has two distinct physical states of binding to lipid bilayers. At low peptide-to-lipid ratios (P/L), t...

journal_title：Biochemistry

authors： Huang HW

更新日期：2000-07-25 00:00:00

abstract：:Four polypeptide chains composing acetylcholine receptors from the electric organ of Torpedo californica were purified by preparative electrophoresis in sodium dodecyl sulfate. Their apparent mole ratio alpha/beta/gamma/delta is 2:1:1:1. These chains are not readily distinguished by amino acid or carbohydrate composit...

journal_title：Biochemistry

authors： Lindstrom J,Merlie J,Yogeeswaran G

更新日期：1979-10-16 00:00:00

abstract：:HlyC is an internal protein acyltransferase that activates hemolysin, a toxic protein produced by pathogenic Escherichia coli. Acyl-acyl carrier protein (ACP) is the essential acyl donor. Separately subcloned, expressed, and purified prohemolysin A (proHlyA), HlyC, and [1-14C]myristoyl-ACP have been used to study the ...

journal_title：Biochemistry

authors： Trent MS,Worsham LM,Ernst-Fonberg ML

更新日期：1999-03-16 00:00:00

abstract：:We constructed and expressed chimeric receptor cDNAs with insulin receptor exon 3 (residues 191-297 of the cysteine-rich region) replaced with either the comparable region of the insulin-like growth factor I receptor (IGF-IR) or the insulin receptor related receptor (IRR). Both chimeric receptors still could bind insu...

journal_title：Biochemistry

authors： Zhang B,Roth RA

更新日期：1991-05-28 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors that exhibit photochromism between two states: a thermally stable dark-adapted state and a metastable light-adapted state with bound linear tetrapyrrole (bilin) chromophores possessing 15Z and 15E configurations, respectively. The photodynamics of canonica...

journal_title：Biochemistry

authors： Kirpich JS,Chang CW,Franse J,Yu Q,Escobar FV,Jenkins AJ,Martin SS,Narikawa R,Ames JB,Lagarias JC,Larsen DS

更新日期：2021-01-13 00:00:00

abstract：:Intrinsic factor (IF(50)) is a cobalamin (Cbl)-transporting protein of 50 kDa, which can be cleaved into two fragments: the 30 kDa N-terminal peptide IF(30) and the 20 kDa C-terminal glycopeptide IF(20). Experiments on binding of Cbl to IF(30), IF(20), and IF(50) revealed comparable association rate constants (k(+)(Cb...

journal_title：Biochemistry

authors： Fedosov SN,Fedosova NU,Berglund L,Moestrup SK,Nexø E,Petersen TE

更新日期：2005-03-08 00:00:00

abstract：:The synthesis of gangliosides GM3, GM2, GM1, and GD1a solely lacking the fatty acid moiety, and thus called lysogangliosides in analogy to lysophospholipids, is described. Since a selective elimination of the fatty acid residue has not been achieved as yet, the gangliosides were first subjected to alkaline hydrolysis....

journal_title：Biochemistry

authors： Neuenhofer S,Schwarzmann G,Egge H,Sandhoff K

更新日期：1985-01-15 00:00:00