Toward an understanding of the mechanism of nonphotochemical quenching in green plants.

abstract：:The gene encoding enzyme IIA(glc) (EIIA) of the phosphoenolpyruvate:sugar phosphotransferase system of Mycoplasma capricolum was cloned into a regulated expression vector. The purified protein product of the overexpressed gene was characterized as an active phosphoacceptor from HPr with a higher pI than previously des...

journal_title：Biochemistry

authors： Zhu PP,Nosworthy N,Ginsburg A,Miyata M,Seok YJ,Peterkofsky A

更新日期：1997-06-10 00:00:00

abstract：:Treatment of ADP-glucose pyrophosphorylase (EC 2.7.7.27) from the cyanobacterium Anabaena PCC 7120 with phenylglyoxal in 50 mM Hepes, pH 8.0, at 25 degrees C resulted in a time- and concentration-dependent loss of enzyme activity. Phosphate, the inhibitor, protected the enzyme from inactivation most effectively, while...

journal_title：Biochemistry

authors： Sheng J,Preiss J

更新日期：1997-10-21 00:00:00

abstract：:This paper reports on NMR-molecular mechanics structural studies of the (-)-trans-anti-[MC]dG adduct positioned opposite dC in the sequence context of the d(C1-C2-A3-T4-C5-[MC]G6-C7-T8-A9-C10-C11).d(G12-G13-T14++ +-A15-G16-C17-G18- A19-T20-G21-G22) duplex [designated (-)-trans-anti-[MC]dG.dC 11-mer duplex]. This adduc...

journal_title：Biochemistry

authors： Cosman M,Xu R,Hingerty BE,Amin S,Harvey RG,Geacintov NE,Broyde S,Patel DJ

更新日期：1995-05-09 00:00:00

abstract：:Aminopeptidase B (APB, EC 3.4.11.6) preferentially hydrolyzes the N-terminal basic amino acids of synthetic and peptide substrates and requires a physiological concentration of NaCl for optimal activity. In this study, we used site-directed mutagenesis and molecular modeling to search for an amino acid residue that is...

journal_title：Biochemistry

authors： Ohnishi A,Watanabe J,Ogawa Y,Goto Y,Hattori A,Tsujimoto M

更新日期：2015-10-06 00:00:00

abstract：:Crystallographic studies of ligand-protein complexes reveal most preferable ligand binding modes, but do not show less populated modes that may contribute to measurable biochemical and biophysical characteristics of the complexes. In some cases, a ligand may bind a protein in essentially different modes. An example is...

journal_title：Biochemistry

authors： Blanchet J,Lin SX,Zhorov BS

更新日期：2005-05-17 00:00:00

abstract：:Surface-enhanced resonance Raman scattering (SERRS) spectra were obtained from carotenoids, in the all-trans configuration, located on the antenna complexes of Rhodobacter sphaeroides 2.4.1 membranes. Since resonance Raman (RR) spectra are barely detectable at the concentration that SERRS signals saturate, SERRS repre...

journal_title：Biochemistry

authors： Picorel R,Lu T,Holt RE,Cotton TM,Seibert M

更新日期：1990-01-23 00:00:00

abstract：:Hydrophobic interaction of 8-anilino-1-naphthalene sulfonic acid (ANS) with proteins is one of the widely used methods for characterizing/detecting partially folded states of proteins. We have carried out a systematic investigation on the effect of ANS, a charged hydrophobic fluorescent dye, on structural properties o...

journal_title：Biochemistry

authors： Ali V,Prakash K,Kulkarni S,Ahmad A,Madhusudan KP,Bhakuni V

更新日期：1999-10-12 00:00:00

abstract：:Extracellular cAMP induces the rapid activation of guanylate cyclase, which adapts within 10 s to constant cAMP concentrations. A new response can be induced either by a higher cAMP concentration or by the same cAMP concentration at some time (t1/2 = 90 s) after removal of the previous stimulus. Stimulation of guanyla...

journal_title：Biochemistry

authors： Van Haastert PJ

更新日期：1987-11-17 00:00:00

abstract：:A study of the regular secondary structure elements of recombinant human interleukin-1 beta has been carried out using NMR spectroscopy. Using a randomly 15N labeled sample, a number of heteronuclear three- and two-dimensional NMR experiments have been performed, which have enabled a complete analysis of short-, mediu...

journal_title：Biochemistry

authors： Driscoll PC,Gronenborn AM,Wingfield PT,Clore GM

更新日期：1990-05-15 00:00:00

abstract：:Cofilin is an actin regulatory protein that binds to both monomeric and filamentous actin, and has filament severing activity. Although crystal structures for the monomeric forms of both G-actin and cofilin have been described, the structure of the binary cofilin-G-actin complex is not available. Synchrotron protein f...

journal_title：Biochemistry

authors： Guan JQ,Vorobiev S,Almo SC,Chance MR

更新日期：2002-05-07 00:00:00

abstract：:The active site topography of rabbit CYP4B1 has been studied relative to CYP2B1 and CYP102 using a variety of aromatic probe substrates. Oxidation of the prochiral substrate cumene by CYP4B1, but not CYP2B1 or CYP102, resulted in the formation of the thermodynamically disfavored omega-hydroxy metabolite, 2-phenyl-1-pr...

journal_title：Biochemistry

authors： Henne KR,Fisher MB,Iyer KR,Lang DH,Trager WF,Rettie AE

更新日期：2001-07-24 00:00:00

abstract：:The Escherichia coli ribosomal protein S4 is known to repress translation of its own gene and several other ribosomal protein (r-protein) genes in the alpha operon as part of a general mechanism coordinating the levels of rRNA and r-protein synthesis. Using a filter binding assay and RNA transcripts prepared in vitro,...

journal_title：Biochemistry

authors： Deckman IC,Draper DE

更新日期：1985-12-31 00:00:00

abstract：:Kinases play a critical role in cellular signaling and are dysregulated in a number of diseases, such as cancer, diabetes, and neurodegeneration. Therapeutics targeting kinases currently account for roughly 50% of cancer drug discovery efforts. The ability to explore human kinase biochemistry and biophysics in the lab...

journal_title：Biochemistry

authors： Albanese SK,Parton DL,Işık M,Rodríguez-Laureano L,Hanson SM,Behr JM,Gradia S,Jeans C,Levinson NM,Seeliger MA,Chodera JD

更新日期：2018-08-07 00:00:00

abstract：:Post-translational phosphorylation and the related conformational changes in signaling proteins are responsible for regulating a wide range of subcellular processes. Human Pin1 is central to many of these cell signaling pathways in normal and aberrant subcellular processes, catalyzing cis-trans isomerization of the pe...

journal_title：Biochemistry

authors： Velazquez HA,Hamelberg D

更新日期：2011-11-08 00:00:00

abstract：:Staphylococcal nuclease H124L is a recombinant protein produced in Escherichia coli whose sequence is identical with that of the nuclease produced by the V8 variant of Staphylococcus aureus. The enzyme-metal ion activator-nucleotide inhibitor ternary complex, nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+, was inves...

journal_title：Biochemistry

authors： Wang JF,LeMaster DM,Markley JL

更新日期：1990-01-09 00:00:00

abstract：:Transcarboxylase is made up of a central hexameric subunit (S20,W similar 12 S), three peripheral dimeric metallo subunits (S20,W similar to 5 S), and six biotinyl carboxyl carrier subunits (S20,W similar to 1.3 S). The results presented here show that the carboxyl carrier subunit is required for assembly of the 12S a...

journal_title：Biochemistry

authors： Ahmad F,Jacobson B,Chuang M,Brattin W,Wood HG

更新日期：1975-04-22 00:00:00

abstract：:All eight cysteine residues, C90, C94, C143, C147, C219, C325, C367, and C431, present in transmembrane domains of the Aspergillus nidulans NrtA nitrate transporter protein were altered individually by site-specific mutagenesis. The results indicate that six residues, C90, C147, C219, C325, C367, and C431, are not req...

journal_title：Biochemistry

authors： Unkles SE,Rouch DA,Wang Y,Siddiqi MY,Okamoto M,Stephenson RM,Kinghorn JR,Glass AD

更新日期：2005-04-12 00:00:00

abstract：:We have studied the photoreaction of 8-methoxypsoralen (8-MOP), 4,5',8-trimethylpsoralen (TMP), and 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) with a pair of 18-base-long oligonucleotides in which a 14-base region is complementary. Only one 5'TpA site, favored for both monoadduct and cross-link formation with p...

journal_title：Biochemistry

authors： Yeung AT,Jones BK,Chu CT

更新日期：1988-05-03 00:00:00

abstract：:Phytochromes are widespread red/far-red photosensory proteins well known as critical regulators of photomorphogenesis in plants. It is often assumed that natural selection would have optimized the light sensing efficiency of phytochromes to minimize nonproductive photochemical deexcitation pathways. Surprisingly, the ...

journal_title：Biochemistry

authors： Kim PW,Rockwell NC,Martin SS,Lagarias JC,Larsen DS

更新日期：2014-05-06 00:00:00

abstract：:The flexibility of individual amino acid side chains of pancreatic phospholipase A2 in aqueous and micellar solutions was studied with deuterium nuclear magnetic resonance (2H NMR). Bovine pancreatic phospholipase A2 was selectively deuterated at the aromatic ring systems of Trp-3 and Phe-5 and porcine pancreatic phos...

journal_title：Biochemistry

authors： Allegrini PR,van Scharrenburg GJ,Slotboom AJ,de Haas GH,Seelig J

更新日期：1985-06-18 00:00:00

abstract：:We have studied carbon monoxide (CO) migration and binding in the nonsymbiotic hemoglobins AHb1 and AHb2 of Arabidopsis thaliana using Fourier transform infrared (FTIR) spectroscopy combined with temperature derivative spectroscopy (TDS) at cryogenic temperatures. Both proteins have similar amino acid sequences but di...

journal_title：Biochemistry

authors： Nienhaus K,Dominici P,Astegno A,Abbruzzetti S,Viappiani C,Nienhaus GU

更新日期：2010-09-07 00:00:00

abstract：:Periodate-oxidized tRNA(Phe) (tRNA(oxPhe)) behaves as a specific affinity label of tetrameric Escherichia coli phenylalanyl-tRNA synthetase (PheRS). Reaction of the alpha 2 beta 2 enzyme with tRNA(oxPhe) results in the loss of tRNAPhe aminoacylation activity with covalent attachment of 2 mol of tRNA dialdehyde/mol of ...

journal_title：Biochemistry

authors： Hountondji C,Schmitter JM,Beauvallet C,Blanquet S

更新日期：1987-08-25 00:00:00

abstract：:A 600 MHz 1H NMR study of toxin OSK1, blocker of small-conductance Ca2+-activated K+ channels, is presented. The unambiguous sequential assignment of all the protons of the toxin was obtained using TOCSY, DQF-COSY, and NOESY experiments at pH 3.0 (10, 30, and 45 degrees C) in aqueous solution. 3J(N alpha), 3J(alphabet...

journal_title：Biochemistry

authors： Jaravine VA,Nolde DE,Reibarkh MJ,Korolkova YV,Kozlov SA,Pluzhnikov KA,Grishin EV,Arseniev AS

更新日期：1997-02-11 00:00:00

abstract：:The Escherichia coli D-galactose and D-glucose receptor possesses a Ca(II)-binding site closely related in structure and metal-binding characteristics to the eukaryotic EF-hand sites. Only the structure of the Ca(II)-occupied site is known. To investigate the structural change triggered by Ca(II) and Sr(II) binding, w...

journal_title：Biochemistry

authors： Luck LA,Falke JJ

更新日期：1991-04-30 00:00:00

abstract：:We have employed molecular dynamics (MD) simulation to investigate, with atomic details, the structural dynamics and energetics of three major ATPase states (ADP, APO, and ATP state) of a human kinesin-1 monomer in complex with a tubulin dimer. Starting from a recently solved crystal structure of ATP-like kinesin-tubu...

journal_title：Biochemistry

authors： Chakraborty S,Zheng W

更新日期：2015-01-27 00:00:00

abstract：:Mismatches in DNA occur naturally during replication and as a result of endogenous DNA damaging agents, but the mismatch repair (MMR) pathway acts to correct mismatches before subsequent rounds of replication. Rhodium metalloinsertors bind to DNA mismatches with high affinity and specificity and represent a promising ...

journal_title：Biochemistry

authors： Ernst RJ,Komor AC,Barton JK

更新日期：2011-12-20 00:00:00

abstract：:The noncovalent interaction of 2-aminodipyrido[1,2-a:3',2'-d]imidazole (Glu-P-2) and its derivatives, which are potent mutagens isolated from L-glutamic acid pyrolysate, with calf thymus DNA was studied by steady-state and nanosecond fluorescence spectroscopies. The fluorescence of these compounds exhibits static quen...

journal_title：Biochemistry

authors： Itoh M,Kurokawa H,Usui M,Ohno M,Shimoda E,Hashimoto Y,Shudo K

更新日期：1985-11-05 00:00:00

abstract：:Previous studies have demonstrated that lung-specific proteins are associated with surfactant lipids, particularly the highly surface active subfraction known as tubular myelin. We have isolated a surfactant-associated protein complex with molecular weight components of 36 000, 32 000, and 28 000 and reassembled it wi...

journal_title：Biochemistry

authors： Hawgood S,Benson BJ,Hamilton RL Jr

更新日期：1985-01-01 00:00:00

abstract：:p21-activated kinases (PAKs) serve as effector proteins for the GTP-binding proteins Cdc42 and Rac. They are serine/threonine kinases containing the Cdc42/Rac interactive binding (CRIB) motif. The main aim of this study was to define the minimal domain of alphaPAK required for Cdc42/Rac binding. Eight stable PAK fragm...

journal_title：Biochemistry

authors： Thompson G,Owen D,Chalk PA,Lowe PN

更新日期：1998-05-26 00:00:00

abstract：:Group I intron RNAs contain a core of highly conserved helices flanked by peripheral domains that stabilize the core structure. In the Tetrahymena group I ribozyme, the P4, P5, and P6 helices of the core pack tightly against a three-helix subdomain called P5abc. Chemical footprinting and the crystal structure of the T...

journal_title：Biochemistry

authors： Doherty EA,Herschlag D,Doudna JA

更新日期：1999-03-09 00:00:00