Abstract:
:When partially purified Ehrlich ascites tumor RNA polymerase II was further purified on a column of phosphocellulose, stimulation of its catalysis of RNA synthesis by stimulatory factor S-II was greatly decreased. This decrease in sensitivity to the stimulatory factor was reversible: the enzyme eluted from phosphocellulose became sensitive to the factor when mixed with a protein fraction eluted from the phosphocellulose at high salt concentration. Evidence was obtained that this protein, named helper protein, binds, to the enzyme eluted from phosphocellulose, causing it to recover sensitivity to stimulatory factor S-II.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kuroiwa A,Mizuno D,Natori Sdoi
10.1021/bi00645a006subject
Has Abstractpub_date
1977-12-27 00:00:00pages
5687-91issue
26eissn
0006-2960issn
1520-4995journal_volume
16pub_type
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