Activation of soluble guanylyl cyclase by four-coordinate metalloporphyrins: evidence for a role for porphyrin conformation.

abstract：:Peptide NH resonances in the 250 MHZ 1H nuclear magnetic resonance (NMR) spectrum of oxytocin in H2O were assigned to specific amino acid residues by the "underwater decoupling" technique (i.e., decoupling from corresponding CalphaH resonances, which are buried beneath the intense water peak). These experiments confir...

journal_title：Biochemistry

authors： Glickson JD,Rowan R,Pitner TP,Dadok J,Bothner-By AA,Walter R

更新日期：1976-03-09 00:00:00

abstract：:Standard preparations of Escherichia coli RNA polymerase harbor a 70 kDa protein with NTPase (beta-gamma cleavage) activity that is not a recognized polymerase subunit. The NTPase activity of this component, before and after separation from the polymerase, is strongly dependent on the presence of DNA; single-stranded ...

journal_title：Biochemistry

authors： Butzow JJ,Garland C,Van Lee L,Eichhorn GL

更新日期：1997-12-02 00:00:00

abstract：:DNA polymerase β (Pol β) is a key enzyme in mammalian base excision repair (BER), contributing stepwise 5'-deoxyribose phosphate (dRP) lyase and "gap-filling" DNA polymerase activities. The lyase reaction is believed to occur via a β-elimination reaction following the formation of a Schiff base between the dRP group a...

journal_title：Biochemistry

authors： Daskalova SM,Bhattacharya C,Dedkova LM,Hecht SM

更新日期：2017-01-24 00:00:00

abstract：:HIV-1 Vif is an accessory protein that induces the proteasomal degradation of the host restriction factor, apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G). The N-terminal half of Vif binds to APOBEC3G, and the C-terminal half binds to subunits of a cullin 5-based ubiquitin ligase. This Vi...

journal_title：Biochemistry

authors： Techtmann SM,Ghirlando R,Kao S,Strebel K,Maynard EL

更新日期：2012-03-13 00:00:00

abstract：:The gene 5 protein (g5p) encoded by filamentous Ff phages is an ssDNA-binding protein, which binds to and sequesters the nascent ssDNA phage genome in the process of phage morphogenesis. The g5p also binds with high affinity to DNA and RNA sequences that form G-quadruplex structures. However, sequences that would form...

journal_title：Biochemistry

authors： Wen JD,Gray DM

更新日期：2004-03-09 00:00:00

abstract：:It is well-known that the rapid flow of water into and out of cells is controlled by membrane proteins called aquaporins (AQPs). However, the mechanisms that allow cells to quickly respond to a changing osmotic environment are less well established. Using GFP-AQP fusion proteins expressed in HEK293 cells, we demonstra...

journal_title：Biochemistry

authors： Conner MT,Conner AC,Brown JE,Bill RM

更新日期：2010-02-09 00:00:00

abstract：:Many bacteria produce isonitrile-containing natural products that are derived from aromatic amino acids. The synthetic clusters that control biosynthesis most commonly encode two enzymes, designated PvcA and PvcB, as well as additional enzymes that direct synthesis of the natural product. The PvcA enzyme installs the ...

journal_title：Biochemistry

authors： Zhu J,Lippa GM,Gulick AM,Tipton PA

更新日期：2015-04-28 00:00:00

abstract：:Insulin-stimulated glucose disposal in skeletal muscle proceeds predominantly through a nonoxidative pathway with glycogen synthase as a rate-limiting enzyme, yet the mechanisms for insulin activation of glycogen synthase are not understood despite years of investigation. Isolation of putative insulin second messenger...

journal_title：Biochemistry

authors： Brautigan DL,Brown M,Grindrod S,Chinigo G,Kruszewski A,Lukasik SM,Bushweller JH,Horal M,Keller S,Tamura S,Heimark DB,Price J,Larner AN,Larner J

更新日期：2005-08-23 00:00:00

abstract：:Class I major histocompatibility complex (MHC) proteins present peptide antigens to T cells during the immune response against viruses. Peptides are loaded into newly synthesized class I heterodimers in the endoplasmic reticulum such that most or all cell surface class I molecules contain peptides derived from endogen...

journal_title：Biochemistry

authors： Fahnestock ML,Johnson JL,Feldman RM,Tsomides TJ,Mayer J,Narhi LO,Bjorkman PJ

更新日期：1994-07-05 00:00:00

abstract：:Alignment of more than 20 deduced sequences for mevalonate diphosphate decarboxylase (MDD) indicates that serines 34, 36, 120,121, 153, and 155 are invariant residues that map within a proposed interdomain active site cleft. To test possible active site roles for these invariant serines, each has been mutated to alani...

journal_title：Biochemistry

authors： Krepkiy DV,Miziorko HM

更新日期：2005-02-22 00:00:00

abstract：:Drug efflux and enzymatic drug degradation are two cellular mechanisms that contribute to drug resistance in many cancers. Herein, we report the synthesis and in vitro activity of a pro-immunostimulant that exploits both processes in tandem to selectively confer cancer-mediated immunogenicity. We demonstrate that an i...

journal_title：Biochemistry

authors： Burt AJ,Hantho JD,Nielsen AE,Mancini RJ

更新日期：2018-04-17 00:00:00

abstract：:The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzy...

journal_title：Biochemistry

authors： Halling SM,Sanchez-Anzaldo FJ,Fukuda R,Doi RH,Meares CF

更新日期：1977-06-28 00:00:00

abstract：:Methylmalonyl-CoA mutase from Propionibacterium shermanii is an adenosylcobalamin-dependent enzyme which catalyzes the reversible isomerization of methylmalonyl-CoA and succinyl-CoA. The rate of tritium loss from 5'-[3H]adenosylcobalamin during the enzymic reaction and the relative rates of tritium appearance in subst...

journal_title：Biochemistry

authors： Meier TW,Thomä NH,Leadlay PF

更新日期：1996-09-10 00:00:00

abstract：:Acetylcholinesterase (AChE) contains a narrow and deep active site gorge with two sites of ligand binding, an acylation site (or A-site) at the base of the gorge and a peripheral site (or P-site) near the gorge entrance. The P-site contributes to catalytic efficiency by transiently binding substrates on their way to t...

journal_title：Biochemistry

authors： Rosenberry TL,Sonoda LK,Dekat SE,Cusack B,Johnson JL

更新日期：2008-12-09 00:00:00

abstract：:Solutions of porcine lactate dehydrogenase of micromolar concentration kept at 4 degrees C for several days lose the greater part of their enzymic activity but recover it when returned to room temperature. The rate of spoiling decreases and the rate of recovery increases with the concentration of the solutions. The de...

journal_title：Biochemistry

authors： King L,Weber G

更新日期：1986-06-17 00:00:00

abstract：:The primary electron acceptor pheophytin (Pheo(D1)) plays a crucial role in regulation of forward and backward electron transfer in photosystem II (PSII). It is known that some cyanobacteria control the Pheo(D1) potential in high-light acclimation by exchanging the D1 proteins from different copies of the psbA genes. ...

journal_title：Biochemistry

authors： Shibuya Y,Takahashi R,Okubo T,Suzuki H,Sugiura M,Noguchi T

更新日期：2010-01-26 00:00:00

abstract：:Silk fibroin exists in a number of different states, such as silk I and silk II, with different properties largely defined by differences in secondary structure composition. Numerous attempts have been made to control the transitions from silk I to silk II in vitro to produce high-performance materials. Of all the fac...

journal_title：Biochemistry

authors： Zhou P,Xie X,Knight DP,Zong XH,Deng F,Yao WH

更新日期：2004-09-07 00:00:00

abstract：:The dissociation of Semliki Forest virus induced by increasing concentrations of the anionic detergent sodium dodecyl sulfate was studied using density gradient centrifugation. Detectable binding to the virus started well below the critical micellar concentration of the detergent and increased thereafter with increase...

journal_title：Biochemistry

authors： Becker R,Helenius A,Simons K

更新日期：1975-05-06 00:00:00

abstract：:The transmission of regulatory signals between the alpha- and beta-subunits of the tryptophan synthase alpha 2 beta 2 complex from Salmonella typhimurium has been investigated by monitoring the luminescence properties of the enzyme in the presence and in the absence of the alpha-subunit ligand DL-alpha-glycerol 3-phos...

journal_title：Biochemistry

authors： Strambini GB,Cioni P,Peracchi A,Mozzarelli A

更新日期：1992-08-25 00:00:00

abstract：:Perturbations within the transactivation and carboxy-terminal domains of HIV-1 Rev were examined for effects on Rev responsive element (RRE) binding activities in vitro and biological activity in vivo. Binding affinities, specificities, and multimerization of the transactivation mutants M10 and Rev/Rex M10-16 on the R...

journal_title：Biochemistry

authors： Daly TJ,Rennert P,Lynch P,Barry JK,Dundas M,Rusche JR,Doten RC,Auer M,Farrington GK

更新日期：1993-08-31 00:00:00

abstract：:The reaction center protein D1 in photosystem II shows a high turnover during illumination. The degradation of the D1 protein is preceded by photoinhibition of the electron transport in photosystem II. There are two distinct mechanisms for this: acceptor-side- and donor-side-induced photoinhibition. Here, donor-side-i...

journal_title：Biochemistry

authors： Jegerschöld C,Styring S

更新日期：1996-06-18 00:00:00

abstract：:Polynucleosomes with poly(dG-m5dC).poly(dG-m5dC) have been reconstituted, and well-defined nucleosome core particles from these have been prepared. Upon addition of MgCl2 to the levels used to induce the B to Z transition in this highly methylated DNA, significant changes in the circular dichroism spectrum are observe...

journal_title：Biochemistry

authors： Ausio J,Zhou G,van Holde K

更新日期：1987-09-08 00:00:00

abstract：:Inducible nitric oxide synthase (iNOS) catalyzes the NADPH-dependent formation of nitric oxide (NO) and citrulline from L-arginine and O2. In addition to serving as substrate, L-arginine alters the enzyme's heme iron spin equilibrium, increases its NADPH oxidation, and promotes assembly of active dimeric iNOS from ina...

journal_title：Biochemistry

authors： Sennequier N,Stuehr DJ

更新日期：1996-05-07 00:00:00

abstract：:Histone H3 arginine 2 (H3R2) is post-translationally modified in three different states by "writers" of the protein arginine methyltransferase (PRMT) family. H3R2 methylarginine isoforms include PRMT5-catalyzed monomethylation (me1) and symmetric dimethylation (me2s) and PRMT6-catalyzed me1 and asymmetric dimethylatio...

journal_title：Biochemistry

authors： Lorton BM,Harijan RK,Burgos ES,Bonanno JB,Almo SC,Shechter D

更新日期：2020-10-06 00:00:00

abstract：:The photoaffinity reagent 6-azido-L-tryptophan was synthesized by chemical methods. It binds reversibly in the dark to the alpha 2 beta 2 complex of tryptophan synthase of Escherichia coli and forms a quinonoid intermediate with enzyme-bound pyridoxal phosphate (lambda max = 476 nm). The absorbance of this chromophore...

journal_title：Biochemistry

authors： Miles EW,Phillips RS

更新日期：1985-08-13 00:00:00

abstract：:C-type natriuretic peptide (CNP) binds the guanylyl cyclase-linked natriuretic peptide receptor B (NPR-B) and stimulates marked elevations of the intracellular signaling molecule, cGMP. Here, the essential role of phosphorylation in the hormonal activation and deactivation of this receptor is described. Exposure of NI...

journal_title：Biochemistry

authors： Potter LR

更新日期：1998-02-24 00:00:00

abstract：:During T cell signaling, Itk selectively phosphorylates a tyrosine within its own SH3 domain and a tyrosine within PLCgamma1. We find that the remote SH2 domain in each of these substrates is required to achieve efficient tyrosine phosphorylation by Itk and extend this observation to two other Tec family kinases, Btk ...

journal_title：Biochemistry

authors： Joseph RE,Min L,Xu R,Musselman ED,Andreotti AH

更新日期：2007-05-08 00:00:00

abstract：:The interaction of heparan sulfate with different ligand proteins depends on the precise location of O-sulfate groups in the polysaccharide chain. We have previously shown that overexpression in human kidney 293 cells of a mouse mastocytoma 2-O-sulfotransferase (2-OST), previously thought to catalyze the transfer of s...

journal_title：Biochemistry

authors： Rong J,Habuchi H,Kimata K,Lindahl U,Kusche-Gullberg M

更新日期：2001-05-08 00:00:00

abstract：:The ability of DNA topoisomerase I to mediate the formation of structurally modified DNA duplexes was studied utilizing suicide substrates containing high-efficiency cleavage sites and acceptor oligonucleotides in which the 5'-terminal nucleotides were varied. When the substrates were nicked duplexes, the divalent cat...

journal_title：Biochemistry

authors： Wang X,Henningfeld KA,Hecht SM

更新日期：1998-02-24 00:00:00

abstract：:We have studied the effects of site-directed mutations in Escherichia coli nitrate reductase A (NarGHI) on heme reduction by a menaquinol analogue (menadiol) using the stopped-flow method. For NarGHI(H66Y) and NarGHI(H187Y), both lacking heme b(L) but having heme b(H), the heme reduction by menadiol is abolished. For ...

journal_title：Biochemistry

authors： Zhao Z,Rothery RA,Weiner JH

更新日期：2003-12-09 00:00:00