Conformational changes of an exchangeable apolipoprotein, apolipophorin III from Locusta migratoria, at low pH: correlation with lipid binding.

abstract：:Fluorescent nucleobase analogues are used extensively to probe the structure and dynamics of nucleic acids. The fluorescence of the adenine analogue 2-aminopurine and the cytosine analogue pyrrolocytosine is significantly quenched when the bases are located in regions of double-stranded nucleic acids. To allow more de...

journal_title：Biochemistry

authors： Hardman SJ,Thompson KC

更新日期：2006-08-01 00:00:00

abstract：:Under suitable experimental conditions the aerobic oxidation of NADH catalyzed by horseradish peroxidase occurred in four characteristic phases: initial burst, induction phase, steady state, and termination. A trace amount of H2O2 present in the NADH solution brought about initial burst in the formation of oxyperoxida...

journal_title：Biochemistry

authors： Yokota K,Yamazaki I

更新日期：1977-05-03 00:00:00

abstract：:Mammalian type B (mitochondrial) b(5) cytochromes exhibit greater amino acid sequence diversity than their type A (microsomal) counterparts, as exemplified by the type B proteins from human (hCYB5B) and rat (rCYB5B). The comparison of X-ray crystal structures of hCYB5B and rCYB5B reported herein reveals a striking dif...

journal_title：Biochemistry

authors： Parthasarathy S,Altuve A,Terzyan S,Zhang X,Kuczera K,Rivera M,Benson DR

更新日期：2011-06-21 00:00:00

abstract：:Aqueous suspensions of dark-adapted bacteriorhodopsin (bR560) in the purple membrane of Halobacterium halobium are exposed to rapid jumps to high pH. Optical and resonance Raman measurements are carried out by using flow and stationary methods. Above pH congruent to 11.5 bR560 starts to be reversibly converted to a sp...

journal_title：Biochemistry

authors： Druckmann S,Ottolenghi M,Pande A,Pande J,Callender RH

更新日期：1982-09-28 00:00:00

abstract：:A comparison of the oxidations of diclofenac with microsomes of yeasts expressing various human liver cytochromes P450 showed that P450 2C9 regioselectively led to 4'-hydroxy diclofenac (4'-OHD) whereas P450 3A4 only led to 5-hydroxy diclofenac (5-OHD). P450 2C19, 2C18, and 2C8 led to the simultaneous formation of 4'-...

journal_title：Biochemistry

authors： Mancy A,Antignac M,Minoletti C,Dijols S,Mouries V,Duong NT,Battioni P,Dansette PM,Mansuy D

更新日期：1999-10-26 00:00:00

abstract：:Ethylene and its analogues acetylene, carbon monoxide, and propylene inhibited the rate of oxidation of indole-3-acetic acid by peroxidase. Annulment of this effect by addition of superoxide dismutase showed that inhibition occurred only in the presence of the superoxide anion radical (O2-.). Kinetic and spectral data...

journal_title：Biochemistry

authors： Smith AM,Morrison WL,Milham PJ

更新日期：1983-03-29 00:00:00

abstract：:The field of chemical site-selective modification of proteins has progressed extensively in recent decades to enable protein functionalization for imaging, drug delivery, and functional studies. In this Perspective, we provide detailed insight into an alternative use of site-selective protein chemistry to probe the ro...

journal_title：Biochemistry

authors： Bertoldo JB,Terenzi H,Hüttelmaier S,Bernardes GJL

更新日期：2018-10-30 00:00:00

abstract：:The structure of [14-38]Abu, a variant of bovine pancreatic trypsin inhibitor (BPTI) with only the 14-38 disulfide bridge intact, has been analyzed by two-dimensional 1H and 1H-15N NMR. Except for the 18-24, 29-35 antiparallel beta-sheet, residues in all regions of the molecule give two exchange cross peaks for each 1...

journal_title：Biochemistry

authors： Barbar E,Barany G,Woodward C

更新日期：1995-09-12 00:00:00

abstract：:Glycine oxidase gene from a strain of Bacillus subtilis was cloned and expressed in Escherichia coli. The purified enzyme was found, by mass spectrometry, to have a protein M(r) of 40763 (value of 40761.6 predicted from DNA sequence) and a FAD prosthetic group M(r) of 785.1 (theoretical value of 785.5). Glycine oxidas...

journal_title：Biochemistry

authors： Jamil F,Gardner QA,Bashir Q,Rashid N,Akhtar M

更新日期：2010-08-31 00:00:00

abstract：:Gram-negative bacteria, such as Escherichia coli, utilize efflux resistance systems in order to expel toxins from their cells. Heavy-metal resistance is mediated by resistance nodulation cell division (RND)-based efflux pumps composed of a tripartite complex that includes an RND-transporter, an outer-membrane factor (...

journal_title：Biochemistry

authors： Mealman TD,Zhou M,Affandi T,Chacón KN,Aranguren ME,Blackburn NJ,Wysocki VH,McEvoy MM

更新日期：2012-08-28 00:00:00

abstract：:alpha5beta1 integrin can occupy several distinct conformational states which support different strengths of binding to fibronectin [García, A. J., et al. (1998) J. Biol. Chem. 273, 34710-34715]. Using a model system in which specific activating monoclonal antibodies were used to achieve uniform activated states, the b...

journal_title：Biochemistry

authors： García AJ,Schwarzbauer JE,Boettiger D

更新日期：2002-07-23 00:00:00

abstract：:Type II dihydrofolate reductases (DHFRs) encoded by the R67 and R388 plasmids are different both in sequence and in structure from known chromosomal DHFRs. These plasmid-derived DHFRs are responsible for conferring trimethoprim resistance to the host strain. A derivative of R388 DHFR, RBG200, has been cloned and overp...

journal_title：Biochemistry

authors： Brito RM,Reddick R,Bennett GN,Rudolph FB,Rosevear PR

更新日期：1990-10-23 00:00:00

abstract：:The bovine protein tyrosine phosphatase (BPTP) is a member of the class of low-molecular weight protein tyrosine phosphatases (PTPases) found to be ubiquitous in mammalian cells. The catalytic site of BPTP contains a CX(5)R(S/T) phosphate-binding motif or P-loop (residues 12-19) which is the signature sequence for all...

journal_title：Biochemistry

authors： Tabernero L,Evans BN,Tishmack PA,Van Etten RL,Stauffacher CV

更新日期：1999-09-07 00:00:00

abstract：:Amphotericin B (AmB) is a polyene macrolide antibiotic isolated from Streptomyces nodosus. The antifungal activity of AmB can be attributed to the formation of an ion-channel assembly in the presence of ergosterol (Erg), in which there are two different AmB-Erg orientations, parallel and antiparallel, as reported prev...

journal_title：Biochemistry

authors： Nakagawa Y,Umegawa Y,Matsushita N,Yamamoto T,Tsuchikawa H,Hanashima S,Oishi T,Matsumori N,Murata M

更新日期：2016-06-21 00:00:00

abstract：:The proton nuclear magnetic resonance spectrum of human adult deoxyhemoglobin in D2O in the region from 6 to 20 ppm downfield from the proton resonance of residual water shows a number of hyperfine shifted proton resonances that are due to groups on or near the alpha and beta hemes. The sensitivity of these resonances...

journal_title：Biochemistry

authors： Viggiano G,Ho NT,Ho C

更新日期：1979-11-13 00:00:00

abstract：:Opossum (Didelphis virginiana) serum was fractionated with (NH4)2SO4 and then chromatographed on DEAE-Sepharose and phenyl-Sepharose. Affinity chromatography on a protein A-Sepharose-antibody column removed traces of opossum serum metalloproteinase inhibitors, and resulted in a homogeneous preparation of opossum alpha...

journal_title：Biochemistry

authors： Catanese JJ,Kress LF

更新日期：1993-01-19 00:00:00

abstract：:Kinetic studies of reactions between fluorescein and antifluorescein antibody produced during early, intermediate, and late stages of the immune response have been carried out utilizing both fluorescence intensity and polarization measurements in the static (time constant similar to 5 sec) and in the stopped-flow mode...

journal_title：Biochemistry

authors： Levison SA,Hicks AN,Portmann AJ,Dandliker WB

更新日期：1975-08-26 00:00:00

abstract：:We have previously shown that both recombinant apo(a) and native Lp(a) inhibit the binding of Glu-plasminogen to fibrin surfaces [Fleury & Anglés-Cano (1991) Biochemistry 30, 7630-7638; Rouy et al. (1992) Biochemistry 31, 6332-6339]. The aim of the present study was to characterize the mechanism of this inhibition and...

journal_title：Biochemistry

authors： Hervio L,Durlach V,Girard-Globa A,Anglés-Cano E

更新日期：1995-10-17 00:00:00

abstract：:Pancreatic phospholipase A(2) (PLA2) shows a strong preference for the binding to the anionic interface and a consequent allosteric activation. In this paper, we show that virtually all the preference is mediated through 3 (Lys-53, -56, and -120) of the 12 cationic residues of bovine pancreatic PLA2. The lysine-to-met...

journal_title：Biochemistry

authors： Yu BZ,Poi MJ,Ramagopal UA,Jain R,Ramakumar S,Berg OG,Tsai MD,Sekar K,Jain MK

更新日期：2000-10-10 00:00:00

abstract：:The effect of myosin on the structure of two sequences on G-actin, a loop between residues 39 and 52 and a segment between residues 61 and 69 from the NH2-terminus, was probed by limited proteolytic digestions of G-actin in the presence of the myosin subfragment 1 isozyme S-1(A2). Under the experimental conditions of ...

journal_title：Biochemistry

authors： Chen T,Haigentz M Jr,Reisler E

更新日期：1992-03-24 00:00:00

abstract：:The photoaffinity reagent 6-azido-L-tryptophan was synthesized by chemical methods. It binds reversibly in the dark to the alpha 2 beta 2 complex of tryptophan synthase of Escherichia coli and forms a quinonoid intermediate with enzyme-bound pyridoxal phosphate (lambda max = 476 nm). The absorbance of this chromophore...

journal_title：Biochemistry

authors： Miles EW,Phillips RS

更新日期：1985-08-13 00:00:00

abstract：:P-glycoprotein (P-gp) is the most well-known ATP-binding cassette (ABC) transporter involved in unidirectional substrate translocation across the membrane lipid bilayer, thereby causing the typical multidrug resistance (MDR) phenotype expressed in many cancers. We observed that in human CEM acute lymphoblastic leukemi...

journal_title：Biochemistry

authors： Gayet L,Dayan G,Barakat S,Labialle S,Michaud M,Cogne S,Mazane A,Coleman AW,Rigal D,Baggetto LG

更新日期：2005-03-22 00:00:00

abstract：:A fluorescent derivative of lysine-258 isolated from the active site of aspartate aminotransferase modified by treatment of the apoenzyme with pyridoxal 5'-sulfate has been characterized as a substituted 2H-pyrrolo[3,4-c]pyridine. Similar pyrrolopyridines are produced in up to 20% yield by reaction of pyridoxal sulfat...

journal_title：Biochemistry

authors： Scott RD,Ueno H,Metzler DE

更新日期：1982-10-12 00:00:00

abstract：:While calcium binding to troponin C (TnC) triggers the contraction of both skeletal and cardiac muscle, there is clear evidence that different mechanisms may be involved. For example, activation of heart myofilaments occurs with binding to a single regulatory site on TnC, whereas activation of fast skeletal myofilamen...

journal_title：Biochemistry

authors： Spyracopoulos L,Li MX,Sia SK,Gagné SM,Chandra M,Solaro RJ,Sykes BD

更新日期：1997-10-07 00:00:00

abstract：:Two successive steps in de novo purine biosynthesis are catalyzed by the enzymes 5-aminoimidazole ribonucleotide (AIR) carboxylase and 4-[(N-succinylamino)carbonyl]-5-aminoimidazole ribonucleotide (SAICAR) synthetase. Amino acid sequence alignments of the proteins from various sources suggested that several unusual di...

journal_title：Biochemistry

authors： Firestine SM,Davisson VJ

更新日期：1994-10-04 00:00:00

abstract：:Proteins performing multiple biochemical functions are called "moonlighting proteins" or extreme multifunctional (EMF) proteins. Mitochondrial cytochrome c is an EMF protein that binds multiple partner proteins to act as a signaling molecule, transfers electrons in the respiratory chain, and acts as a peroxidase in ap...

journal_title：Biochemistry

authors： Deacon OM,Karsisiotis AI,Moreno-Chicano T,Hough MA,Macdonald C,Blumenschein TMA,Wilson MT,Moore GR,Worrall JAR

更新日期：2017-11-21 00:00:00

abstract：:Aquaporin-4 (AQP4) is the predominant water channel in the brain. Although AQP4 plays an important role in brain water homeostasis, the molecular mechanisms of AQP4 regulation are not fully understood. In this report, we show how Zn(2+) rapidly and reversibly decreases the water permeability of AQP4 when it is reconst...

journal_title：Biochemistry

authors： Yukutake Y,Hirano Y,Suematsu M,Yasui M

更新日期：2009-12-29 00:00:00

abstract：:Phospholipase A2 from Naja naja kaouthia venom was covalently coupled onto agarose beads using two different chemistries. The effect of micellar competitive inhibitors in the coupling media was evaluated. Enzyme bound to N-hydroxysuccinimide-activated agarose, which is reactive primarily toward epsilon-amino groups, h...

journal_title：Biochemistry

authors： Ferreira JP,Sasisekharan R,Louie O,Langer R

更新日期：1993-08-17 00:00:00

abstract：:The mammalian iron-binding proteins lactoferrin (Lf) and transferrin (Tf) bind iron very tightly, but reversibly. Despite homologous structures and essentially identical iron binding sites, Tf begins to release iron at pH 6.0, whereas Lf retains iron to pH approximately 3.5. This difference in iron retention gives the...

journal_title：Biochemistry

authors： Peterson NA,Arcus VL,Anderson BF,Tweedie JW,Jameson GB,Baker EN

更新日期：2002-12-03 00:00:00

abstract：:Histidine-containing protein, HPr, is a phosphocarrier protein that is part of the bacterial phosphoenolpyruvate:sugar phosphotransferase system. HPr is phosphorylated by enzyme I, and P-HPr transfers the phosphoryl group to the IIA domain of a number of sugar-specific enzyme II complexes. Autocatalytic phosphoryl tra...

journal_title：Biochemistry

authors： Anderson JW,Waygood EB

更新日期：1993-06-08 00:00:00