The structure of a soluble chemoreceptor suggests a mechanism for propagating conformational signals.

abstract：:The peptidyl trifluoromethyl ketones Ac-Phe-CF3 (1) and Ac-Leu-Phe-CF3 (2) are inhibitors of chymotrypsin. They differ in Ki (20 and 2 microM, respectively) as well as in their kinetics of association with chymotrypsin in that 1 is rapidly equilibrating, with an association rate too fast to be observed by steady-state...

journal_title：Biochemistry

authors： Brady K,Wei AZ,Ringe D,Abeles RH

更新日期：1990-08-21 00:00:00

abstract：:The nitrophorins are a family of proteins that use ferric heme to transport nitric oxide (NO) from the salivary glands of blood-sucking insects to their victims, resulting in vasodilation and reduced blood coagulation. We have refined atomic resolution structures of nitrophorin 4 (NP4) from Rhodnius prolixus complexed...

journal_title：Biochemistry

authors： Roberts SA,Weichsel A,Qiu Y,Shelnutt JA,Walker FA,Montfort WR

更新日期：2001-09-25 00:00:00

abstract：:Flash-induced Fourier transform infrared (FTIR) difference spectra for the four-step S-state cycle and the effects of global (15)N- and (13)C-isotope labeling on the difference spectra were examined for the first time in the mid- to low-frequency (1200-800 cm(-1)) as well as the mid-frequency (1700-1200 cm(-1)) region...

journal_title：Biochemistry

authors： Yamanari T,Kimura Y,Mizusawa N,Ishii A,Ono TA

更新日期：2004-06-15 00:00:00

abstract：:Two sites of ligand interaction in acetylcholinesterase (AChE) were first demonstrated in ligand binding studies and later confirmed by crystallography, site-specific mutagenesis, and molecular modeling: an acylation site at the base of the active site gorge and a peripheral site at its mouth. We recently introduced a...

journal_title：Biochemistry

authors： Szegletes T,Mallender WD,Thomas PJ,Rosenberry TL

更新日期：1999-01-05 00:00:00

abstract：:The phytocyanins are a family of plant cupredoxins that have been subdivided into the stellacyanins, plantacyanins, and uclacyanins. All of these proteins possess the typical type 1 His(2)Cys equatorial ligand set at their mononuclear copper sites, but the stellacyanins have an axial Gln ligand in place of the weakly ...

journal_title：Biochemistry

authors： Harrison MD,Yanagisawa S,Dennison C

更新日期：2005-03-01 00:00:00

abstract：:The recognition sequence of the Myb protein has been recently described to be pyAACKGHH (where py = T/C, K = G/T, and H = A/C/T), modifying the earlier identification as pyAACKG [Ording, E., et al. (1994) Eur. J. Biochem. 222, 113-120]. We had earlier determined the solution structure of the minimal cognate sequence T...

journal_title：Biochemistry

authors： Radha PK,Patel PK,Hosur RV

更新日期：1998-07-14 00:00:00

abstract：:Oxygenic photosynthesis in plants involves highly reactive intermediates and byproducts that can damage the photosynthetic apparatus and other chloroplast constituents. The potential for damage is exacerbated when the amount of absorbed light exceeds the capacity for light energy utilization in photosynthesis, a condi...

journal_title：Biochemistry

authors： Holt NE,Fleming GR,Niyogi KK

更新日期：2004-07-06 00:00:00

abstract：:Enzyme-substrate contacts in the hydrolysis of ester substrates by the cysteine protease papain were investigated by systematically altering backbone hydrogen-bonding and side-chain hydrophobic contacts in the substrate and determining each substrate's kinetic constants. The observed specificity energies [defined as d...

journal_title：Biochemistry

authors： Berti PJ,Faerman CH,Storer AC

更新日期：1991-02-05 00:00:00

abstract：:Intrastrand cross-links (IaCL) connecting two purine nucleobases in DNA pose a challenge to high-fidelity replication in the cell. Various repair pathways or polymerase bypass can cope with these lesions. The influence of the phosphodiester linkage between two neighboring 2'-deoxyguanosine (dG) residues attached throu...

journal_title：Biochemistry

authors： O'Flaherty DK,Guengerich FP,Egli M,Wilds CJ

更新日期：2015-12-29 00:00:00

abstract：:It was previously observed that two steps of the reaction of eukaryotic DNA topoisomerase I (topoisomerization and cleavage) depend upon the conformation of the DNA substrate: in both instances the supercoiled form is a more efficient substrate than the relaxed one. This paper reports the analysis of two other steps o...

journal_title：Biochemistry

authors： Caserta M,Amadei A,Camilloni G,Di Mauro E

更新日期：1990-09-04 00:00:00

abstract：:Design of a partially cysteine-depleted C98S/C239S/C377S/C468A cytochrome P450 3A4 mutant designated CYP3A4(C58,C64) allowed site-directed incorporation of thiol-reactive fluorescent probes into alpha-helix A. The site of modification was identified as Cys-64 with the help of CYP3A4(C58) and CYP3A4(C64), each bearing ...

journal_title：Biochemistry

authors： Tsalkova TN,Davydova NY,Halpert JR,Davydov DR

更新日期：2007-01-09 00:00:00

abstract：:One-dimensional 1H NMR was employed to study the effects of Ca2+ and Lu3+ binding on the apo and calcium-saturated forms of dog bone Gla protein (BGP, osteocalcin). Titration of apo dog BGP with Ca2+ in 20 mM NaCl showed spectral perturbations consistent with the binding of 5 mol equiv of calcium in the NMR slow-excha...

journal_title：Biochemistry

authors： Isbell DT,Du S,Schroering AG,Colombo G,Shelling JG

更新日期：1993-10-26 00:00:00

abstract：:We have described earlier a chromatin-bound protease with unique specificity for histone H2A [Eickbush, T. H., Watson, D. K., & Moudrianakis, E. N. (1976) Cell (Cambridge, Mass.) 9, 785--792]. In the present study, we explore the nature of interactions that form and stabilize the enzyme-chromatin system by using the a...

journal_title：Biochemistry

authors： Watson DK,Moudrianakis EN

更新日期：1982-01-19 00:00:00

abstract：:Phosphatidylserine (PS) in the plasma membrane of nonactivated human platelets is almost entirely located on the cytoplasmic side. Stimulation of platelets with the Ca2+ ionophore A23187 or combined action of collagen plus thrombin results in a rapid loss of the asymmetric distribution of PS. Also, treatment with the ...

journal_title：Biochemistry

authors： Bevers EM,Tilly RH,Senden JM,Comfurius P,Zwaal RF

更新日期：1989-03-21 00:00:00

abstract：:The structure of the ca. 250 amino acid hormone binding domain of the human estrogen receptor (hER-LBD), expressed in E. coli and purified as a complex with estradiol, has been probed by selective proteolysis, with analysis of the protein fragments both by classical methods (SDS-PAGE and Edman N-terminal sequencing) a...

journal_title：Biochemistry

authors： Seielstad DA,Carlson KE,Kushner PJ,Greene GL,Katzenellenbogen JA

更新日期：1995-10-03 00:00:00

abstract：:Crude calf thymus DNA-dependent RNA polymerase, RNA polymerase B (ribonucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6), was incubated with the tritium labeled, potent inhibitor [3H]amanin, in order to form the enzymatically inactive [3H]amanin-polymerase complex ([3H]A-P complex). Subsequent purificati...

journal_title：Biochemistry

authors： Brodner OG,Wieland T

更新日期：1976-08-10 00:00:00

abstract：:The di-heme enzyme MauG catalyzes the oxidative biosynthesis of a tryptophan tryptophylquinone cofactor on a precursor of the enzyme methylamine dehydrogenase (preMADH). Reaction of H2O2 with the diferric form of MauG, or reaction of O2 with diferrous MauG, forms the catalytic intermediate known as bis-Fe(IV), which a...

journal_title：Biochemistry

authors： Yukl ET,Williamson HR,Higgins L,Davidson VL,Wilmot CM

更新日期：2013-12-31 00:00:00

abstract：:Cysteine desulfurases (CDs) are pyridoxal-5'-phosphate (PLP)-dependent enzymes that cleave sulfur from cysteine via an enzyme cysteinyl persulfide intermediate. In vitro studies of these enzymes have generally employed dithiothreitol as a cosubstrate to reductively cleave the persulfide intermediate, and it has been s...

journal_title：Biochemistry

authors： Behshad E,Parkin SE,Bollinger JM Jr

更新日期：2004-09-28 00:00:00

abstract：:Chloride intracellular channel protein 1 (CLIC1) functions as an anion channel in plasma and nuclear membranes when its soluble monomeric form converts to an integral-membrane form. The transmembrane region of CLIC1 is located in its thioredoxin-like domain 1, but the mechanism whereby the protein converts to its memb...

journal_title：Biochemistry

authors： Stoychev SH,Nathaniel C,Fanucchi S,Brock M,Li S,Asmus K,Woods VL Jr,Dirr HW

更新日期：2009-09-08 00:00:00

abstract：:A specific tertiary hydrogen bond that is present between the side-chain hydroxyl group of Tyr30 and the side-chain N delta 1 atom of His17 in the small, monomeric, single-domain protein, barstar, has been perturbed by site-directed mutagenesis of the sole histidine residue (His17) to a glutamine residue. The effect o...

journal_title：Biochemistry

authors： Nath U,Udgaonkar JB

更新日期：1995-02-07 00:00:00

abstract：:The secretory component (SC) isolated from human milk was labeled with 2 mol of the fluorescent thiol reagent N-[7-(dimethylamino)-4-methylcoumarinyl]maleimide (DACM) per mol of SC through the reactive disulfide bond of SC. The binding of the labeled SC to polymeric immunoglobulins was examined by gel filtration by me...

journal_title：Biochemistry

authors： Goto Y,Aki K

更新日期：1984-12-18 00:00:00

abstract：:The alpha-helix formed by the amino acid residues 9-19 of the B-chain of insulin is involved in the stabilization of its three-dimensional structure. We have shown that modification at positions B9, B10, B12, and B16 results in analogues possessing biological activities ranging from ca. 0.2% to ca. 500% relative to th...

journal_title：Biochemistry

authors： Hu SQ,Burke GT,Schwartz GP,Ferderigos N,Ross JB,Katsoyannis PG

更新日期：1993-03-16 00:00:00

abstract：:The heme-regulated inhibitor (HRI) of protein synthesis becomes activated in rabbit reticulocyte lysates in response to a variety of conditions including heme-deficiency, addition of oxidants, and heat shock. Activated HRI inhibits translation by catalyzing the phosphorylation of the alpha-subunit of eukaryotic initia...

journal_title：Biochemistry

authors： Matts RL,Hurst R,Xu Z

更新日期：1993-07-27 00:00:00

abstract：:1,3-15N-Labeled uracil was synthesized chemically and used to prepare labeled Escherichia coli tRNA(Val) biosynthetically. 500-MHz measurements of 15N and proton chemical shift were obtained, for all uridine and uridine-related bases, by heteronuclear multiple-quantum coherence spectroscopy. All the uracil NH group re...

journal_title：Biochemistry

authors： Choi BS,Redfield AG

更新日期：1992-12-29 00:00:00

abstract：:Members of the phospholipase C-beta (PLC-beta) family of proteins are activated either by G alpha or G beta gamma subunits of heterotrimeric G proteins. To define specific regions of PLC-beta 3 that are involved in binding and activation by G beta gamma, a series of fragments of PLC-beta 3 as glutathione-S-transferase...

journal_title：Biochemistry

authors： Barr AJ,Ali H,Haribabu B,Snyderman R,Smrcka AV

更新日期：2000-02-22 00:00:00

abstract：:Distinct structural features of DNA, such as the curvature of dA tracts, are important in the recognition, packaging, and regulation of DNA. Physiologically relevant concentrations of magnesium have been found to enhance the curvature of dA tract DNAs, as monitored by solution-state NMR, indicating that the structure ...

journal_title：Biochemistry

authors： Jerkovic B,Bolton PH

更新日期：2001-08-07 00:00:00

abstract：:DNA-dependent synthesis of beta-galactosidase was optimized in extracts made from cells lysed by a standard French pressure cell. Extracts made at 3200 psi synthesized up to 25-fold more beta-galactosidase than extracts made at 7500 psi. beta-Galactosidase synthesis was cyclic 3', 5' AMP dependent, as expected, and in...

journal_title：Biochemistry

authors： Jacobs KA,Schlessinger D

更新日期：1977-03-08 00:00:00

abstract：:We previously showed that beta-amyloid precursor protein (APP) is cleaved not only in the middle of the membrane (gamma-cleavage) but also at novel cleavage sites close to the membrane/cytoplasmic boundary (epsilon-cleavage), releasing APP intracellular domains (AICDs) 49-99 and 50-99. To learn more about the relation...

journal_title：Biochemistry

authors： Funamoto S,Morishima-Kawashima M,Tanimura Y,Hirotani N,Saido TC,Ihara Y

更新日期：2004-10-26 00:00:00

abstract：:The local and global dynamics of the chemokine receptor CXCR1 are characterized using a combination of solution NMR and solid-state NMR experiments. In isotropic bicelles (q = 0.1), only 13% of the expected number of backbone amide resonances is observed in (1)H/(15)N HSQC solution NMR spectra of uniformly (15)N-label...

journal_title：Biochemistry

authors： Park SH,Casagrande F,Das BB,Albrecht L,Chu M,Opella SJ

更新日期：2011-03-29 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) allows the study of interactions of fluorescently labeled ligand with receptors in living cells at single-molecule detection sensitivity. From the autocorrelation functions of fluorescence intensity fluctuations, the diffusion time of molecules through the confocal volume is...

journal_title：Biochemistry

authors： Pramanik A,Olsson M,Langel U,Bartfai T,Rigler R

更新日期：2001-09-11 00:00:00