Abstract：
:The membrane-bound state of the gene 9 minor coat protein of bacteriophage M13 was studied in various membrane-mimicking systems, including organic solvents, detergent micelles, and phospholipid bilayers. For this purpose we determined the conformational and aggregational properties of the chemically synthesized protein by CD, FTIR, and HPSEC. The protein appears to be in a monomeric or small oligomeric alpha-helical state in TFE but adopts a mixture of alpha-helical and random structure after subsequent incorporation into SDS or DOPG. When solubilized by sodium cholate, however, the protein undergoes a transition in time into large aggregates, which contain mainly beta-sheet conformation. The rate of this beta-polymerization process was decreased at lower temperature and higher concentrations of sodium cholate. This aggregation was reversed only upon addition of high concentrations of the strong detergent SDS. By reconstitution of the cholate-solubilized protein into DOPG, it was found that the state of the protein, whether initially alpha-helical monomeric/oligomeric or beta-sheet aggregate, did not change. On the basis of our results, we propose that the principal conformational state of membrane-bound gene 9 protein in vivo is alpha-helical.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Houbiers MC,Spruijt RB,Wolfs CJ,Hemminga MAdoi
10.1021/bi981149esubject
Has Abstractpub_date
1999-01-19 00:00:00pages
1128-35issue
3eissn
0006-2960issn
1520-4995pii
bi981149ejournal_volume
38pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:FKBP12, a small human enzyme, aids protein folding by catalyzing cis-trans isomerization of peptidyl-prolyl bonds, and is involved in cell signaling pathways, calcium regulation, and the immune response. The underlying molecular mechanisms are not fully understood, but it is well-known that aromatic residues in the ac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b01157
更新日期：2017-01-10 00:00:00
abstract：:A core Y61F mutant of the gene 5 single-stranded DNA-binding protein (g5p) of f1 bacterial virus aggregated when expressed from a plasmid, but, after refolding in vitro, it behaved much like wild-type and may be a stability or folding mutant. Circular dichroism (CD) titrations showed the same cooperative polynucleotid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972545k
更新日期：1998-05-19 00:00:00
abstract：:We have examined the effects on redox kinetics of changing the reduction potential of the mu-oxo-bridged binuclear iron center in octameric hemerythrin (Hr) from Phascolopsis gouldii. The opportunity to examine such effects is provided by the availability of mu-sulfidomethemerythrin (mu-S2-metHr), whose [Fe(III),Fe(II...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00400a033
更新日期：1987-12-29 00:00:00
abstract：:The ATPase activity of the (Ca2+-Mg2+)-ATPase reconstituted into bilayers of phosphatidylcholines depends on the fatty acyl chain length of the phospholipids. It is shown that the fluorescence response to Ca2+ of the ATPase modified with fluorescein isothiocyanate is also dependent on phospholipid structure and is int...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00371a043
更新日期：1986-11-18 00:00:00
abstract：:Tissue plasminogen activator-inhibitor complexes were purified from the conditioned medium of human umbilical vein endothelial cells by affinity chromatography followed by gel filtration. It was found that a single complex was isolated which can exist in two distinct interconvertible conformations. These may be separa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00397a037
更新日期：1987-11-17 00:00:00
abstract：:Prodan [6-propionyl-2-(dimethylamine)naphthalene] is a hydrophobic fluorescent probe which is extremely sensitive to both the polarity and the hydrogen-bond donating capacity of the solvent. In binary mixtures of solvents, the hydrogen-bond donating effect on Prodan fluorescence saturates at relatively low concentrati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00154a021
更新日期：1992-10-06 00:00:00
abstract：:Substrates homoprotocatechuate (HPCA) and O(2) bind to the Fe(II) of homoprotocatechuate 2,3-dioxygenase (FeHPCD) in adjacent coordination sites. Transfer of an electron(s) from HPCA to O(2) via the iron is proposed to activate the substrates for reaction with each other to initiate aromatic ring cleavage. Here, rapid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201436n
更新日期：2011-11-29 00:00:00
abstract：:The structural and functional properties of G protein-coupled receptors (GPCRs) are often studied in a detergent micellar environment, but many GPCRs tend to denature or aggregate in short alkyl chain detergents. In our previous work [Lee, S., et al. (2016) J. Am. Chem. Soc.138, 15425-15433], we showed that GPCRs in a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00183
更新日期：2020-06-16 00:00:00
abstract：:Membrane vesicles isolated from Escherichia coli ML 308--225 have been analyzed by crossed immunoelectrophoresis, and immunoprecipitates corresponding to the following cellular components have been identified: ATPase (EC 3.6.1,3), two or three NADH dehydrogenases (EC 1.6.99.3), D-lactate dehydrogenase (EC 1.1.1.27), g...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00575a004
更新日期：1979-04-17 00:00:00
abstract：:3-Deoxy-d-manno-octulosonate 8-phosphate (KDO8P) synthase catalyzes the condensation of arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) to form KDO8P, a key precursor in the biosynthesis of the endotoxin of Gram-negative bacteria. Earlier studies have established that the condensation occurs with a syn addit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901341h
更新日期：2009-12-15 00:00:00
abstract：:In prokaryotes, the first three enzymes in pyrimidine biosynthesis, carbamoyl phosphate synthetase (CPS), aspartate transcarbamoylase (ATC), and dihydroorotase (DHO), are commonly expressed separately and either function independently (Escherichia coli) or associate into multifunctional complexes (Aquifex aeolicus). I...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801831r
更新日期：2009-02-03 00:00:00
abstract：:Flash-induced Fourier transform infrared (FTIR) difference spectra for the four-step S-state cycle and the effects of global (15)N- and (13)C-isotope labeling on the difference spectra were examined for the first time in the mid- to low-frequency (1200-800 cm(-1)) as well as the mid-frequency (1700-1200 cm(-1)) region...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0362323
更新日期：2004-06-15 00:00:00
abstract：:Site-directed mutations R177A and R177K in the gene encoding manganese peroxidase isozyme 1 (mnp1) from Phanerochaete chrysosporium were generated. The mutant enzymes were expressed in P. chrysosporium during primary metabolic growth under the control of the glyceraldehyde-3-phosphate dehydrogenase gene promoter, puri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990943c
更新日期：1999-08-31 00:00:00
abstract：:T4 RNA ligase catalyzes the ATP-dependent addition of a single 2'-deoxyribonucleoside 3',5'-bisphosphate to the 3'-hydroxyl of an oligodeoxyribonucleotide. The bisphosphate is joined to the deoxyoligomer by a 3' leads to 5' phosphodiester bond and the product, which is terminated by a 3'-phosphate, is one nucleotide l...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00617a004
更新日期：1978-11-28 00:00:00
abstract：:cAMP binding of the androgen receptor (AR) from murine skeletal muscle was studied. Testosterone affinity chromatography yielded androgen receptor with about 4000-fold purification. Determination of the cAMP binding in the affinity eluate, by adsorption of protein-cAMP complexes to cellulose ester filters or removal o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00423a013
更新日期：1988-11-15 00:00:00
abstract：:The flavin-dependent quiescin-sulfhydryl oxidase (QSOX) inserts disulfide bridges into unfolded reduced proteins with the reduction of molecular oxygen to form hydrogen peroxide. This work investigates how QSOX and protein disulfide isomerase (PDI) cooperate in vitro to generate native pairings in two unfolded reduced...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801604x
更新日期：2008-11-18 00:00:00
abstract：:The Bordetella adenylate cyclase toxin-hemolysin (CyaA, ACT, or AC-Hly) forms cation-selective membrane channels and delivers into the cytosol of target cells an adenylate cyclase domain (AC) that catalyzes uncontrolled conversion of cellular ATP to cAMP. Both toxin activities were previously shown to depend on post-t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050459b
更新日期：2005-09-27 00:00:00
abstract：:The residue C221 on pyruvate decarboxylase (EC. 4.1.1.1) from Saccharomyces cerevisiae has been shown to be the site where the substrate activation cascade is triggered [Baburina et al. (1994) Biochemistry 33, 5630-5635] and is located on the beta domain [Arjunan et al. (1996) J. Mol. Biol. 256, 590], while the active...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9709912
更新日期：1998-02-03 00:00:00
abstract：:Amyloid fibrils have been classically defined as linear, nonbranched polymeric proteins with a cross beta-sheet structure and the ability to alter the optical properties of the amyloid-specific dye Congo Red. Mounting evidence suggests that soluble oligomeric peptide assemblies approximately 2-20 nm in diameter are cr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700247y
更新日期：2007-06-19 00:00:00
abstract：:The folding and unfolding rates of the small protein, barstar, have been monitored using stopped-flow measurements of intrinsic tryptophan fluorescence at 25 degrees C, pH 8.5, and have been compared over a wide range of urea and guanidine hydrochloride (GdnHCl) concentrations. When the logarithms of the rates of fold...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011494v
更新日期：2002-02-05 00:00:00
abstract：:Human DJ-1, a disease-associated protein that protects cells from oxidative stress, contains an oxidation-sensitive cysteine (C106) that is essential for its cytoprotective activity. The origin of C106 reactivity is obscure, due in part to the absence of an experimentally determined p K a value for this residue. We ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800282d
更新日期：2008-07-15 00:00:00
abstract：:Eubacterial peptidyl-tRNA hydrolase (PTH) recycles all N-blocked aminoacyl-tRNA molecules but initiator formyl-methionyl-tRNAfMet, the acceptor helix of which is characterized by a 1-72 mismatch. Positive selection by PTH of noninitiator tRNA molecules with a full 1-72 base pair is abolished, however, upon the removal...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982657r
更新日期：1999-04-20 00:00:00
abstract：:The heterodimeric actin capping protein (CP) is regulated by a set of proteins that contain CP-interacting (CPI) motifs. Outside of the CPI motif, the sequences of these proteins are unrelated and distinct. The CPI motif and surrounding sequences are conserved within a given protein family, when compared to those of o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00092
更新日期：2020-03-24 00:00:00
abstract：:A critical event in Alzheimer's disease is the transition of Abeta peptides from their soluble forms into disease-associated beta-sheet-rich conformers. Structural analysis of a complete D-amino acid replacement set of Abeta(1-42) enabled us to localize in the full-length 42-mer peptide the region responsible for the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002005e
更新日期：2001-05-08 00:00:00
abstract：:Murine epidermal growth factor (EGF), a 53 amino acid protein, has been modified by enzymic digestion, site-specific chemical reactions, and recombinant DNA technology. After trypsin digestion the EGF derivatives EGF1-48 (called EGF-T) and EGF1-45 (called EGF-T2) were separated from the residual EGF and the C-terminal...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00414a005
更新日期：1988-07-12 00:00:00
abstract：:Thermal transitions of type I collagen fibrils were investigated by differential scanning calorimetry and spectrophotometry of turbidity within a wide range of external conditions. The advanced microcalorimeter allowed us to carry out the measurements at low concentrations of collagen (0.15-0.3 mg/mL). At these concen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980360n
更新日期：1998-06-02 00:00:00
abstract：:AMSH, a deubiquitinating enzyme (DUB) with exquisite specificity for Lys63-linked polyubiquitin chains, is an endosome-associated DUB that regulates sorting of activated cell-surface signaling receptors to the lysosome, a process mediated by the members of the endosomal sorting complexes required for transport (ESCRT)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401106b
更新日期：2013-11-05 00:00:00
abstract：:Nonribosomal peptide synthetases (NRPSs) are megasynthetases that require complex and specific interactions between multiple domains and proteins to functionally produce a metabolite. MbtH-like proteins (MLPs) are integral components of many NRPSs and interact directly with the adenylation domain of the megasynthetase...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00552
更新日期：2018-07-17 00:00:00
abstract：:Vitamin K dependent carboxylation of an exogenous peptide substrate and endogenous protein substrates, vitamin K epoxidation, and reduction of vitamin K epoxide were measured in subcellular fractions from rat liver. The rough microsomal fraction was highly enriched in all four activities; lower levels were found in sm...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00547a019
更新日期：1980-03-18 00:00:00
abstract：:Nitroalkane oxidase (NAO) from Fusarium oxysporum catalyzes the oxidation of neutral nitroalkanes to the corresponding aldehydes or ketones with the production of H(2)O(2) and nitrite. The flavoenzyme is a new member of the acyl-CoA dehydrogenase (ACAD) family, but it does not react with acyl-CoA substrates. We presen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051966w
更新日期：2006-01-31 00:00:00
