Interspersion of mouse satellite deoxyribonucleic acid sequences.

abstract：:We have characterized a membrane protein containing residues P688-T762 of the integrin beta3 subunit, encompassing its transmembrane and cytoplasmic domains, by nuclear magnetic resonance spectroscopy. Under conditions in which it is monomeric in dodecylphosphocholine micelles, the protein consists mainly of alpha-hel...

journal_title：Biochemistry

authors： Li R,Babu CR,Valentine K,Lear JD,Wand AJ,Bennett JS,DeGrado WF

更新日期：2002-12-31 00:00:00

abstract：:A likely entry/exit path for nitrogenase substrates, products, and/or protons involves residues α277(Arg), α192(Ser), and α356(Gly), all of which are highly conserved among MoFe proteins from different organisms. The α192(Ser) and α277(Arg) residues form part of a hydrogen-bonded network that also involves α195(His), ...

journal_title：Biochemistry

authors： Fisher K,Hare ND,Newton WE

更新日期：2014-10-07 00:00:00

abstract：:Using 125I-labeled phenol-alanine sparsomycin, an analogue of sparsomycin having higher biological activity than the unmodified antibiotic, we studied the requirements and the characteristics of its interaction with the ribosome. The drug does not bind to either isolated ribosomal subunits or reconstituted whole ribos...

journal_title：Biochemistry

authors： Lazaro E,van den Broek LA,San Felix A,Ottenheijm HC,Ballesta JP

更新日期：1991-10-08 00:00:00

abstract：:X-ray structural information provides the opportunity to explore quantitatively the relation between the microenvironments of heme proteins and their redox potentials. This can be done by considering the protein as a "solvent" for its redox center and calculating the difference between the electrostatic energy of the ...

journal_title：Biochemistry

authors： Churg AK,Warshel A

更新日期：1986-04-08 00:00:00

abstract：:The bacterial second messenger signaling molecule bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) controls important biological processes such as biofilm formation, virulence response, and motility. This second messenger is sensed by macromolecular targets inside the cell, both protein and RNA, which ind...

journal_title：Biochemistry

authors： Shanahan CA,Gaffney BL,Jones RA,Strobel SA

更新日期：2013-01-15 00:00:00

abstract：:The mutagenic and genotoxic properties of 1,N6-ethenoadenine (epsilon Ade), 3,N4-ethenocytosine (epsilon Cyt), and 4-amino-5-(imidazol-2-yl)imidazole (beta) were investigated in vivo. The former two modified bases are known DNA adducts formed by the human carcinogen vinyl chloride; beta is formed by pyrimidine ring-op...

journal_title：Biochemistry

authors： Basu AK,Wood ML,Niedernhofer LJ,Ramos LA,Essigmann JM

更新日期：1993-11-30 00:00:00

abstract：:A 19-amino acid residue peptide, Gly-Trp-Leu-Lys-Ile-Lys-Ala-Ala-Met-Arg-Trp-Gly-Phe-Phe-Val-Arg-Lys-Lys- Ala, corresponding to the basic amphiphilic alpha-helix (BAA) motif at the C-terminus of a recombinant tobacco calmodulin-binding protein, TCB60, was synthesized. The interaction of the synthetic binding domain wi...

journal_title：Biochemistry

authors： Dash S,Niemaczura W,Harrington HM

更新日期：1997-02-25 00:00:00

abstract：:The non-typeable Haemophilus influenzae strain DH1 was isolated from a 25 year old male patient with Fisher syndrome, a postinfectious autoimmune condition characterized by the presence of anti-GQ1b IgG antibodies that target and initiate damage to peripheral nerves. DH1 was found to display an alphaNeuAc(2-8)alphaNeu...

journal_title：Biochemistry

authors： Houliston RS,Koga M,Li J,Jarrell HC,Richards JC,Vitiazeva V,Schweda EK,Yuki N,Gilbert M

更新日期：2007-07-10 00:00:00

abstract：:A rapid, three-step purification of DNA alpha-polymerase from calf thymus is described. The key feature is immunoaffinity chromatography using a column of immobilized monoclonal immunoglobulin G (IgG) developed against human KB cell alpha-polymerase. This step is followed by preparative sucrose gradient sedimentation....

journal_title：Biochemistry

authors： Wahl AF,Kowalski SP,Harwell LW,Lord EM,Bambara RA

更新日期：1984-04-24 00:00:00

abstract：:The general view on Rhizobium chitolipooligosaccharides (CLOS) is that they are made in very low levels as diffusible molecules and are primarily secreted by the bacteria into the extracellular milieu where they interact with the host. However, the structural and predicted physicochemical properties of these amphiphil...

journal_title：Biochemistry

authors： Orgambide GG,Lee J,Hollingsworth RI,Dazzo FB

更新日期：1995-03-21 00:00:00

abstract：:The inhibition of phosphorylase kinase catalytic activity by 0.1 M neutral salts was predicted by the Hofmeister series of anions. The site of action of the salts was determined by the following evidence to be on the phosphorylase kinase molecule directly, rather than on its protein substrate. (1) Nonactivated kinase ...

journal_title：Biochemistry

authors： Carlson GM,Graves DJ

更新日期：1976-10-05 00:00:00

abstract：:The promoter fragment -1550/+129 of the human hepatic triglyceride lipase (HTGL) gene drives the expression of the CAT gene in HepG2 cells, albeit at very low levels. Transient transfections in HepG2 and HeLa cells of 5' deletion constructs indicated that the regulatory elements that control this expression are locate...

journal_title：Biochemistry

authors： Hadzopoulou-Cladaras M,Cardot P

更新日期：1993-09-21 00:00:00

abstract：:Our previous studies revealed that interaction of fibrin with the very low density lipoprotein (VLDL) receptor plays a prominent role in transendothelial migration of leukocytes and thereby inflammation. The major goal of our subsequent studies is to establish the structural basis for this interaction. As the first st...

journal_title：Biochemistry

authors： Banerjee K,Yakovlev S,Gruschus JM,Medved L,Tjandra N

更新日期：2018-07-31 00:00:00

abstract：:If cytochrome c oxidase is subjected to pressure during the aerobic steady state, large spectral changes are apparent. These seem to be associated with the inhibition of electron transport within the oxidase. The volume change for the transition is about 80 mL/mol. When the oxidase in the aerobic steady state, with po...

journal_title：Biochemistry

authors： Kornblatt JA,Hui Bon Hoa G,Heremans K

更新日期：1988-07-12 00:00:00

abstract：:In clostridial-type ferredoxins, each of the two [4Fe-4S]2+/+ clusters receives three of its four ligands from a CysXXCysXXCys motif. Azotobacter vinelandii ferredoxin I (AvFdI) is a seven-iron ferredoxin that contains one [4Fe-4S]2+/+ cluster and one [3Fe-4S]+/0 cluster. During the evolution of the 7Fe azotobacter-ty...

journal_title：Biochemistry

authors： Kemper MA,Gao-Sheridan HS,Shen B,Duff JL,Tilley GJ,Armstrong FA,Burgess BK

更新日期：1998-09-15 00:00:00

abstract：:In many crustaceans, changing concentrations of several low molecular weight compounds modulates hemocyanin oxygen binding, resulting in lower or higher oxygen affinities of the pigment. The nonphysiological effector caffeine and the physiological modulator urate, the latter accumulating in the hemolymph of the lobste...

journal_title：Biochemistry

authors： Menze MA,Hellmann N,Decker H,Grieshaber MK

更新日期：2005-08-02 00:00:00

abstract：:Bacterial L-asparaginases, enzymes that catalyze the hydrolysis of L-asparagine to aspartic acid, have been used for over 30 years as therapeutic agents in the treatment of acute childhood lymphoblastic leukemia. Other substrates of asparaginases include L-glutamine, D-asparagine, and succinic acid monoamide. In this ...

journal_title：Biochemistry

authors： Aghaiypour K,Wlodawer A,Lubkowski J

更新日期：2001-05-15 00:00:00

abstract：:We propose a simple method to find an amino acid sequence that is foldable into a globular protein with a desired structure based on a knowledge-based 3D-1D compatibility function. An asymmetric alpha-helical single-domain structure of sperm whale myoglobin consisting of 153 amino acid residues was chosen for the desi...

journal_title：Biochemistry

authors： Isogai Y,Ota M,Fujisawa T,Izuno H,Mukai M,Nakamura H,Iizuka T,Nishikawa K

更新日期：1999-06-08 00:00:00

abstract：:Acetylcholinesterase (AChE) contains a narrow and deep active site gorge with two sites of ligand binding, an acylation site (or A-site) at the base of the gorge and a peripheral site (or P-site) near the gorge entrance. The P-site contributes to catalytic efficiency by transiently binding substrates on their way to t...

journal_title：Biochemistry

authors： Rosenberry TL,Sonoda LK,Dekat SE,Cusack B,Johnson JL

更新日期：2008-12-09 00:00:00

abstract：:Compartmentation and NMR visibility of mitochondrial adenine nucleotides were quantitated in isolated rat liver mitochondria respiring on succinate and glutamate in vitro at 8 and 25 degrees C. Intra- and extramitochondrial nucleotides were discriminated by adding the chelator trans-1,2-diaminocyclohexane-N,N,N',N'-te...

journal_title：Biochemistry

authors： Hutson SM,Berkich D,Williams GD,LaNoue KF,Briggs RW

更新日期：1989-05-16 00:00:00

abstract：:We have recently reported that the Na pump alpha-subunit has cytosolic-oriented oligosaccharides which were sensitive to cleavage by an enzyme specific for hydrolysis of N-linked glycans [Pedemonte et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 9789-9793]. We now describe experiments that characterize the saccharides...

journal_title：Biochemistry

authors： Pedemonte CH,Kaplan JH

更新日期：1992-11-03 00:00:00

abstract：:Human DNA primase synthesizes short RNA primers that DNA polymerase alpha then elongates during the initiation of all new DNA strands. Even though primase misincorporates NTPs at a relatively high frequency, this likely does not impact the final DNA product since the RNA primer is replaced with DNA. We used an extensi...

journal_title：Biochemistry

authors： Urban M,Joubert N,Purse BW,Hocek M,Kuchta RD

更新日期：2010-02-02 00:00:00

abstract：:The nonbulky 5',8-cyclopurine DNA lesions (cP) and the bulky, benzo[ a]pyrene diol epoxide-derived stereoisomeric cis- and trans- N2-guanine adducts (BPDE-dG) are good substrates of the human nucleotide excision repair (NER) mechanism. These DNA lesions were embedded at the In or Out rotational settings near the dyad ...

journal_title：Biochemistry

authors： Shafirovich V,Kolbanovskiy M,Kropachev K,Liu Z,Cai Y,Terzidis MA,Masi A,Chatgilialoglu C,Amin S,Dadali A,Broyde S,Geacintov NE

更新日期：2019-02-12 00:00:00

abstract：:Laminins are large multidomain proteins of the extracellular matrix (ECM) with important functions in the development and maintenance of cellular organization and supramolecular structure, in particular in basement membranes. Each molecule is composed of three polypeptide chains, A (300-400 kDa) and B1 and B2 (180-200...

journal_title：Biochemistry

authors： Engel J

更新日期：1992-11-10 00:00:00

abstract：:The AT-rich interaction domain (ARID) containing BAF250a is a subunit of the BAF-A class of SWI/SNF chromatin remodeling complexes. The ARID belongs to a family of conserved DNA binding domains found in several eukaryotic proteins; however, its exact contribution to BAF250a function and the mechanism of its DNA bindin...

journal_title：Biochemistry

authors： Giri M,Maulik A,Singh M

更新日期：2020-01-14 00:00:00

abstract：:A number of isomeric diacylglycerophosphocholines and diacylglycero sulfates containing O-acyl and/or S-acyl ester bonds were investigated as substrates for porcine pancreatic phospholipase A2 and its zymogen. A comparison is made with the kinetic properties of the enzyme toward the corresponding glycol detergents pre...

journal_title：Biochemistry

authors： van Oort MG,Dijkman R,Hille JD,de Haas GH

更新日期：1985-12-31 00:00:00

abstract：:The thermodynamic and activation parameters for the reduction of delta 1-pyrroline-2-carboxylic acid (an alpha-imino acid) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) are compared with those for the reduction of the same imino acid by the glutamate dehydrogenase-NADPH complex. The enthalpies of acti...

journal_title：Biochemistry

authors： Srinivasan R,Fisher HF

更新日期：1985-09-24 00:00:00

abstract：:HpaI and BphI are two pyruvate class II aldolases found in aromatic meta-cleavage degradation pathways that catalyze similar reactions but are not related in sequence. Steady-state kinetic analysis of the aldol addition reactions and product inhibition assays showed that HpaI exhibits a rapid equilibrium random order ...

journal_title：Biochemistry

authors： Wang W,Baker P,Seah SY

更新日期：2010-05-04 00:00:00

abstract：:We present spectroscopic evidence for the presence of low-barrier hydrogen bonds (LBHBs) in molecular complexes composed of carboxylic acids and 1-methylimidazole (1-MeIm) dissolved in aprotic organic solvents. A plot of the values of the low-field proton NMR chemical shifts versus the aqueous pKa of the carboxylic ac...

journal_title：Biochemistry

authors： Tobin JB,Whitt SA,Cassidy CS,Frey PA

更新日期：1995-05-30 00:00:00

abstract：:The role of pyridoxal 5'-phosphate (PLP) in the radical-mediated amino group migration catalyzed by lysine 2,3-aminomutase from Clostridia SB4 has been investigated by electron spin echo envelope modulation (ESEEM) spectroscopy. This pulsed electron paramagnetic resonance (EPR) method was used to estimate the distance...

journal_title：Biochemistry

authors： Ballinger MD,Frey PA,Reed GH,LoBrutto R

更新日期：1995-08-08 00:00:00