Abstract:
:The complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) (EC 2.6.1.2) is presented. Two primary sets of overlapping fragments were obtained by cleavage of the pyridylethylated protein at methionyl and lysyl bonds with cyanogen bromide and Achromobacter protease I, respectively. Isolated peptides were analyzed with a protein sequencer or with a plasma desorption time of flight mass spectrometer and placed in the sequence on the basis of their molecular mass and homology to the sequence of rat GPT. The protein was found to be acetylated at the amino terminus and contained 495 amino acid residues. The Mr of the subunit was calculated to be 54,479, which was in good agreement with a Mr of 55,000 estimated by SDS-PAGE, and also indicated that the active enzyme with a Mr of 114,000 was a homodimer composed of two identical subunits. The amino acid sequence is highly homologous to that of rat GPT (87.9% identity) recently determined [Ishiguro, M., Suzuki, M., Takio, K., Matsuzawa, T., & Titani, K. (1991) Biochemistry 30, 6048-6053]. All of the crucial amino acid residues are conserved in human GPT, which seem to be hydrogen bonding to pyridoxal 5'-phosphate in rat GPT by the sequence homology to other alpha-aminotransferases with known tertiary structures.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Ishiguro M,Takio K,Suzuki M,Oyama R,Matsuzawa T,Titani Kdoi
10.1021/bi00107a013subject
Has Abstractpub_date
1991-10-29 00:00:00pages
10451-7issue
43eissn
0006-2960issn
1520-4995journal_volume
30pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::S100A6 is a member of the S100 subfamily of EF-hand Ca (2+) binding proteins that has been shown to interact with calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP or SIP), a subunit of an SCF-like E3 ubiquitin ligase complex (SCF-TBL1) formed under genotoxic stress. SIP serves as a scaffold in this com...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801233z
更新日期:2008-10-14 00:00:00
abstract::The binding of ethidium bromide, as monitored by fluorescence enhancement, to chromatin prepared by nuclease digestion has been compared with the binding of the dye to sheared chromatin. The nuclease preparation (native chromatin) is characterized by a high affinity region of the Scatchard plot (r = 0-0.025, K1 = 1 X ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00622a002
更新日期:1977-02-08 00:00:00
abstract::Currently, the relatively high cost of enzymes such as glycoside hydrolases that catalyze cellulose hydrolysis represents a barrier to commercialization of a biorefinery capable of producing renewable transportable fuels such as ethanol from abundant lignocellulosic biomass. Among the many families of glycoside hydrol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100009p
更新日期:2010-04-20 00:00:00
abstract::The agouti-related protein gene (Agrp) plays an important role in body weight regulation. The mature human protein is a single polypeptide chain of 112 amino acid residues, consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. The disulfide structure of recombinant human AGRP was dete...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981082v
更新日期:1998-09-01 00:00:00
abstract::The virally encoded integrase protein carries out retroviral integration, which requires specific interactions with the two ends of the viral DNA, and also with host DNA that is the target of integration. We attached a photo-cross-linking agent to specific viral and target DNA sites to identify regions of the integras...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972949c
更新日期:1998-05-12 00:00:00
abstract::The rotational motions of immunoglobulin M (IgM) were investigated by the nanosecond fluorescence depolarization technique. The fluorophore epsilon-1-dimethylamino-5-naphthalenesulfonyl-L-lysine (DNS-lysine) was specifically bound in the combining sites of anti-DNS IgM antibodies from the horse, pig, and nurse shark. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00660a033
更新日期:1976-07-27 00:00:00
abstract::The bacterial chemoreceptor complex governs signal detection and the upstream elements of chemotactic behavior, but the detailed molecular mechanism is still unclear. We have assembled nativelike functional arrays of an aspartate receptor cytoplasmic fragment (CF) with its two cytoplasmic protein partners (CheA and Ch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00234
更新日期:2016-07-05 00:00:00
abstract::The transcriptional activity of the beta-globin genes is regulated by a complex genetic element, the locus control region (LCR), at the 5'-end of the beta-globin locus. Tandem binding sites for the erythroid-specific transcription factor NF-E2 are important for the transcriptional activation function of the LCR. We di...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00050a015
更新日期:1995-12-19 00:00:00
abstract::The essential active site Fe3+ of protocatechuate 3,4-dioxygenase [3, 4-PCD, subunit structure (alphabetaFe3+)12] is bound by axial ligands, Tyr447 (147beta) and His462 (162beta), and equatorial ligands, Tyr408 (108beta), His460 (160beta), and a solvent OH- (Wat827). Recent X-ray crystallographic studies have shown th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972047b
更新日期:1998-02-24 00:00:00
abstract::Reactivation of organophosphate (OP)-inhibited acetylcholinesterase (AChE) is a key objective in the treatment of OP poisoning. This study with native, wild-type, and mutant recombinant DNA-expressed AChEs, each inhibited by representative OP compounds, establishes a relationship between edrophonium acceleration of ox...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9905720
更新日期:1999-08-03 00:00:00
abstract::Optical detection of triplet-state magnetic resonance (ODMR) is employed to study the complexes formed between gene 32 protein (GP32), a single-stranded DNA-binding protein from bacteriophage T4, and the heavy-atom-derivatized polynucleotides poly(5-HgU) and poly(5-BrU). The triplet-state properties of some of the try...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00368a005
更新日期:1986-10-07 00:00:00
abstract::Molecular interactions between the tumor suppressor p53 and the transcriptional coactivators CBP/p300 are critical for the regulation of p53 transactivation and stability. The transactivation domain (TAD) of p53 binds directly to several CBP/p300 domains (TAZ1, TAZ2, NCBD, and KIX). Here we map the interaction between...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi802055v
更新日期:2009-03-17 00:00:00
abstract::Crystallographic studies have demonstrated two flavin conformations for p-hydroxybenzoate hydroxylase (PHBH) [Gatti, D. L., Palfey, B. A. , Lah, M. S., Entsch, B., Massey, V., Ballou, D. P., & Ludwig, M. L. (1994) Science 266, 110-114. Schreuder, H. A., Mattevi, A., Obmolova, G., Kalk, K. H., Hol, W. G. J., van der Bo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971427u
更新日期:1997-12-16 00:00:00
abstract::Myelin basic protein (MBP) binds to negatively charged lipids on the cytosolic surface of oligodendrocyte membranes and is most likely responsible for adhesion of these surfaces in the multilayered myelin sheath. It can also polymerize actin, bundle F-actin filaments, and bind actin filaments to lipid bilayers through...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0519194
更新日期:2006-01-17 00:00:00
abstract::We have characterized the importance of size, sulfation, and anticoagulant activity of heparin in release of basic fibroblast growth factor (bFGF) from the subendothelial extracellular matrix (ECM) and the luminal surface of the vascular endothelium. For this purpose, 125I-bFGF was first incubated with ECM and conflue...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00122a027
更新日期:1992-02-25 00:00:00
abstract::Minor leucine tRNA species, tRNA(Leu)4 and tRNA(Leu)5, from Escherichia coli B have been reported to recognize leucine codons UUA and UUG [Goldman, E., Holmes, W. M., and Hatfield, G. W. (1979) J. Mol. Biol. 129, 567-585]. In the present study, these two tRNA(Leu) species were purified from E. coli A19, and the nucleo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981865g
更新日期:1999-01-05 00:00:00
abstract::Recognition of and binding to cholesterol on the host cell membrane is an initial step in the mechanism of numerous pathogens, including viruses, bacteria, and bacterial toxins; however, a viable method of inhibiting this interaction has not yet been uncovered. Here, we describe the mechanism by which a cholesterol re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00430
更新日期:2016-08-30 00:00:00
abstract::In the accompanying paper [Suzuki, A., Yamazaki, M., & Ito, T. (1996) Biochemistry 35, 5238-5244], we presented a quantitative phase diagram of actin filament ( (F-actin) described with the F-actin concentration and delta chi value which characterizes the affinity of F-actin with solvent. The phase diagram shows that ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9526948
更新日期:1996-04-23 00:00:00
abstract::Common structural motifs, such as the cupin domains, are found in enzymes performing different biochemical functions while retaining a similar active site configuration and structural scaffold. The soil bacterium Bacillus subtilis has 20 cupin genes (0.5% of the total genome) with up to 14% of its genes in the form of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0484421
更新日期:2005-01-11 00:00:00
abstract::13C NMR has been used to demonstrate the metabolism of dilute solutions of labeled formaldehyde by Escherichia coli to methanol, formate, carbon dioxide, and several other unidentified metabolites which contain labeled CH2 groups. Aeration of bacterial suspensions within the spectrometer dramatically increased the rat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00298a017
更新日期:1984-01-31 00:00:00
abstract::Second-harmonic light scattering (SHS) permits characterization of membrane-specific molecular transport in living cells. Herein, we demonstrate the use of time-resolved SHS for quantifying chemically induced enhancements in membrane permeability. As proof of concept, we examine the enhanced permeability of the cytopl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00600
更新日期:2015-07-28 00:00:00
abstract::The mechanism of binding of reduced 1-(2-carboxyphenylamino)-1-deoxyribulose 5-phosphate (rCdRP) to two different binding sites on the bifunctional enzyme is determined by kinetic studies, using temperature-jump and stopped-flow equipment with fluorescence detection. Two rapid binding processes and a comparatively slo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00593a030
更新日期:1979-12-25 00:00:00
abstract::Human telomeric DNA consists of tandem repeats of the DNA sequence d(GGGTTA). Oligodeoxynucleotide telomere models such as d[A(GGGTTA)(3)GGG] (Tel22) fold in a cation-dependent manner into quadruplex structures consisting of stacked G-quartets linked by d(TTA) loops. NMR has shown that in Na(+) solutions Tel22 forms a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901357r
更新日期:2010-01-12 00:00:00
abstract::The enzymes in the catechol meta-fission pathway have been studied for more than 50 years in several species of bacteria capable of degrading a number of aromatic compounds. In a related pathway, naphthalene, a toxic polycyclic aromatic hydrocarbon, is fully degraded to intermediates of the tricarboxylic acid cycle by...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00050
更新日期:2016-05-10 00:00:00
abstract::Enzyme-substrate contacts in the hydrolysis of ester substrates by the cysteine protease papain were investigated by systematically altering backbone hydrogen-bonding and side-chain hydrophobic contacts in the substrate and determining each substrate's kinetic constants. The observed specificity energies [defined as d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00219a033
更新日期:1991-02-05 00:00:00
abstract::Crystallographic analysis revealed that the nicotinamide ring of NAD can bind with multiconformations to aldehyde dehydrogenase (ALDH) (Ni, L., Zhou, J., Hurley, T. D., and Weiner, H. (1999) Protein Sci. 8, 2784-2790). Electron densities can be defined for two conformations, neither of which appears to be compatible w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi012197t
更新日期:2002-06-04 00:00:00
abstract::A simple model system is described that allows measurement of equilibrium Ca2+ binding to multilamellar vesicle mixtures of palmitoyloleoylphosphatidylserine (P,O-PS) and dimyristoleoylphosphatidylcholine (MO,MO-PC). The constraint of the chemical equilibrium among aqueous Ca2+, hydrated P,O-PS/MO,MO-PC, and Ca(PS)2, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00488a013
更新日期:1990-09-11 00:00:00
abstract::The active site topography of rabbit CYP4B1 has been studied relative to CYP2B1 and CYP102 using a variety of aromatic probe substrates. Oxidation of the prochiral substrate cumene by CYP4B1, but not CYP2B1 or CYP102, resulted in the formation of the thermodynamically disfavored omega-hydroxy metabolite, 2-phenyl-1-pr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010395e
更新日期:2001-07-24 00:00:00
abstract::FixL is a sensor histidine kinase having a heme-containing domain as an O(2) sensing site. In the study presented here, Ile209 and Ile210 located near the heme iron of the heme domain of Rhizobium meliloti FixL (RmFixL) were mutated, and the mutational effects on the regulation of the kinase activity and the heme pock...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001184x
更新日期:2000-11-14 00:00:00
abstract::SH2 domain-containing inositol 5-phosphatases 1 (SHIP1) and 2 (SHIP2) are structurally similar proteins that catalyze the degradation of lipid secondary messenger phosphatidylinositol 3,4,5-triphosphate to produce phosphatidylinositol 3,4-diphosphate. Despite their high sequence identity (51%), SHIP1 and SHIP2 share l...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9012462
更新日期:2009-11-24 00:00:00