Abstract:
:The interaction of the zinc finger protein WT1 with RNA aptamers has been investigated using a quantitative binding assay, and the results have been compared to those from a previous study of the DNA binding properties of this protein. A recombinant peptide containing the four zinc fingers of WT1 (WT1-ZFP) binds to representatives of three specific families of RNA aptamers with apparent dissociation constants ranging from 13.8 +/- 1.1 to 87.4 +/- 10.4 nM, somewhat higher than the dissociation constant of 4.12 +/- 0.4 nM for binding to DNA. An isoform that contains an insertion of three amino acids between the third and fourth zinc fingers (WT1[+KTS]-ZFP) also binds to these RNAs with slightly reduced affinity (the apparent dissociation constants ranging from 22.8 to 69.8 nM) but does not bind to DNA. The equilibrium binding of WT1-ZFP to the highest-affinity RNA molecule was compared to the equilibrium binding to a consensus DNA molecule as a function of temperature, pH, monovalent salt concentration, and divalent salt concentration. The interaction of WT1-ZFP with both nucleic acids is an entropy-driven process. Binding of WT1-ZFP to RNA has a pH optimum that is narrower than that observed for binding to DNA. Binding of WT1-ZFP to DNA is optimal at 5 mM MgCl(2), while the highest affinity for RNA was observed in the absence of MgCl(2). Binding of WT1 to both nucleic acid ligands is sensitive to increasing monovalent salt concentration, with a greater effect observed for DNA than for RNA. Point mutations in the zinc fingers associated with Denys-Drash syndrome have dramatically different effects on the interaction of WT1-ZFP with DNA, but a consistent and modest effect on the interaction with RNA. The role of RNA sequence and secondary structure in the binding of WT1-ZFP was probed by site-directed mutagenesis. Results indicate that a hairpin loop is a critical structural feature required for protein binding, and that some consensus nucleotides can be substituted provided proper base pairing of the stem of the hairpin loop is maintained.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Zhai G,Iskandar M,Barilla K,Romaniuk PJdoi
10.1021/bi001941rsubject
Has Abstractpub_date
2001-02-20 00:00:00pages
2032-40issue
7eissn
0006-2960issn
1520-4995pii
bi001941rjournal_volume
40pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::A series of plastocyanin mutants have been constructed by site-directed mutagenesis and expressed in Escherichia coli to elucidate the interaction between plastocyanin and photosystem 1 in the photosynthetic electron-transfer chain. Leu-12 has been replaced with alanine, asparagine, glutamate, and lysine, while Tyr-83...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9520141
更新日期:1996-01-30 00:00:00
abstract::The dimerization specificity of the recombinantly expressed and purified rod domain of adult and neonatal chicken myosin heavy chain was analyzed using metal chelation chromatography. Our results indicate that full-length adult and neonatal rods preferentially formed homodimers when renatured from an equimolar mixture...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060204d
更新日期:2006-04-18 00:00:00
abstract::Porcine pancreatic phospholipase A2 interacts with micelles of the substrate analogue n-octadecylphosphocholine to form a specific complex over considerably wide concentration ranges of both lipid and protein. UV absorption difference spectroscopy measurements indicate that the ratio of lipid to protein molecules in t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00517a019
更新日期:1981-07-07 00:00:00
abstract::AppA, a transcriptional antirepressor, regulates the steady expression of photosynthesis genes in Rhodobacter sphaeroides in response to high-intensity blue light and to redox signals. Its blue-light sensing is mediated by an N-terminal BLUF domain, a member of a novel flavin fold. The photocycle of this domain (AppA(...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051367p
更新日期:2006-01-10 00:00:00
abstract::Blastochloris tepida is a newly described thermophilic purple bacterium containing bacteriochlorophyll b. Using purified light-harvesting 1 reaction center (LH1-RC) core complexes from Blc. tepida, we compared the biochemical, spectroscopic, and thermal denaturation properties of these complexes with those of its meso...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00192
更新日期:2020-06-30 00:00:00
abstract::The interaction of poly[(G-C)] and poly[d(G-m5C)] with the antitumor antibiotic elsamicin A, which binds to alternating guanine + cytosine tracts in DNA, has been studied under the B and Z conformations. Both the rate and the extent of the B-to-Z transition are diminished by the antibiotic, as inferred by spectroscopi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00161a051
更新日期:1992-11-24 00:00:00
abstract::We have combined three mutations previously shown to stabilize lambda repressor against thermal denaturation. Two of these mutations are in helix 3, where Gly-46 and Gly-48 have been replaced by alanines [Hecht, M. H., et al. (1986) Proteins: Struct., Funct., Genet. 1, 43-46]. The other mutation, which replaces Tyr-88...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00419a059
更新日期:1988-09-20 00:00:00
abstract::MenB, the 1,4-dihydroxy-2-naphthoyl-CoA synthase from the bacterial menaquinone biosynthesis pathway, catalyzes an intramolecular Claisen condensation (Dieckmann reaction) in which the electrophile is an unactivated carboxylic acid. Mechanistic studies on this crotonase family member have been hindered by partial acti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200877x
更新日期:2011-11-08 00:00:00
abstract::Recent evidence adds support to a traditional concept according to which the eukaryotic nucleus is organized into functional domains by scaffold or matrix attachment regions (S/MARs). These regions have previously been predicted to have a high potential for stress-induced duplex destabilization (SIDD). Here we report ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026496+
更新日期:2003-01-14 00:00:00
abstract::Lactose repressor protein, regulator of lac enzyme expression in Escherichia coli, maintains its structure and function at extremely low protein concentrations (<10(-)12 M). To examine the unfolding and dissociation of this tetrameric protein, structural transitions in the presence of varying concentrations of urea we...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9900727
更新日期:1999-05-18 00:00:00
abstract::Human and mouse metallothionein-3 (MT-3) molecules exhibit the same metal binding stoichiometry with Zn(II), Cd(II), or Cu(I) as MT-1 or MT-2 molecules, suggesting that MT-3 consists of two domains enfolding separate polymetallic clusters. The kinetic reactivities of Zn(II) complexes of MT-3 with the chelator ethylene...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00014a031
更新日期:1995-04-11 00:00:00
abstract::Sulfation is a Golgi-specific modification of secretory proteins. We have characterized the proteins that are labeled with 35SO4 in cultures of rat hepatocytes and studied their transport to the medium. Analysis by polyacrylamide gel electrophoresis showed that of the five most heavily labeled proteins, four had well-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00435a069
更新日期:1989-05-02 00:00:00
abstract::The clade B/intracellular serpins protect cells from peptidase-mediated injury by forming covalent complexes with their targets. SERPINB12 is expressed in most tissues, especially at cellular interfaces with the external environment. This wide tissue distribution pattern is similar to that of granzyme A (GZMA). Becaus...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b01042
更新日期:2015-11-17 00:00:00
abstract::Protein carboxyl methyltransferases (EC 2.1.1.77) that catalyze the transfer of a methyl group from S-adenosylmethionine to L-isoaspartyl and D-aspartyl residues in a variety of peptides and proteins are widely, but not universally, distributed in nature. These enzymes can participate in the repair of damaged proteins...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00092a020
更新日期:1993-10-19 00:00:00
abstract::An inhibitor of the insulin receptor tyrosine kinase (IRTK), (hydroxy-2-naphthalenyl-methyl) phosphonic acid, was designed and synthesized and was shown to be an inhibitor of the biological effects of insulin in vitro. With a wheat germ purified human placental insulin receptor preparation, this compound inhibited the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00439a053
更新日期:1989-06-27 00:00:00
abstract::Staphylococcal nuclease H124L is a recombinant protein produced in Escherichia coli whose sequence is identical with that of the nuclease produced by the V8 variant of Staphylococcus aureus. The enzyme-metal ion activator-nucleotide inhibitor ternary complex, nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+, was inves...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00453a011
更新日期:1990-01-09 00:00:00
abstract::Photolyases (PHRs) repair the UV-induced photoproducts, cyclobutane pyrimidine dimer (CPD) or pyrimidine-pyrimidone (6-4) photoproduct [(6-4) PP], restoring normal bases to maintain genetic integrity. CPD and (6-4) PP are repaired by substrate-specific PHRs, CPD PHR and (6-4) PHR, respectively. Flavin adenine dinucleo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b01111
更新日期:2016-02-02 00:00:00
abstract::Sedimentation equilibrium results, obtained with bovine zinc-free insulin (with and without a component of proinsulin) at pH 7.0, I o.2, 25 degrees C, and up to a total concentration of 0.8 g/l., are shown to be consistent with three different polymerization patterns, all involving an isodesmic indefinite self-associa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00666a018
更新日期:1976-10-19 00:00:00
abstract::Molecular dynamics simulations employing a molecular mechanics (MM) force field and hybrid quantum mechanics (QM) and MM (QM/MM) have been carried out to investigate the product specificity and mechanism of the histone H4 lysine 20 (H4-K20) methylation by human histone lysine methyltransferase SET8. At neutral pH, the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800244s
更新日期:2008-06-24 00:00:00
abstract::Michler's hydrol blue (MHB) is investigated with respect to photophysical properties in varied solvent environment and when bound to insulin and lysozyme fibrils. The MHB chromophore is shown to act like a molecular rotor and bind well to amyloid fibrils, where it exhibits a characteristic red-shift in its excitation ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi102016p
更新日期:2011-05-03 00:00:00
abstract::Escherichia coli cAMP receptor protein (CRP) controls more than 20 genes. There are significant differences in the promoter regions in these genes. Thus, an elucidation of the mechanism of CRP action requires knowledge about the mode of selectivity in these promoters. An earlier study [Heyduk, T., & Lee, J. C. (1990) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952187q
更新日期:1996-01-30 00:00:00
abstract::Two major neutral glyceroglucolipids (A and B) have been isolated from lipid extract of human gastric content by the procedure involving column fractionation on DEAE-Sephadex, silicic acid, and thin-layer chromatography. Both glycolipids contained glucose, glyceryl ethers, and fatty acids. The structures of these glyc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00637a003
更新日期:1977-09-06 00:00:00
abstract::The interaction of Triton X-100 and other nonionic detergents with a delipidated preparation of the Ca2+ ATPase from sarcoplasmic reticulum has been studied. Binding of radiolabeled Triton X-100 was determined by column chromatography at 6 degrees C, and two classes of binding sites were observed. Below the critical m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00510a006
更新日期:1981-03-31 00:00:00
abstract::The potassium form of d(CGCGAATTCGCG) solved by X-ray diffraction to 1.75 A resolution indicates that monovalent cations penetrate the primary and secondary layers of the "spine of hydration". Both the sodium [Shui, X., McFail-Isom, L., Hu, G. G., and Williams, L. D. (1998) Biochemistry 37, 8341-8355] and the potassiu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982063o
更新日期:1998-12-01 00:00:00
abstract::Human NFU (also known as HIRIP5) has been implicated in cellular iron-sulfur cluster biosynthesis. Bacterial and yeast forms are smaller than the human protein and are homologous to the C-terminal domain of the latter. This C-terminal domain contains a pair of redox active cysteines and demonstrates thioredoxin-like a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801645z
更新日期:2009-02-10 00:00:00
abstract::Thiocyanate reacts noncatalytically with myeloperoxidase-derived HOCl to produce hypothiocyanite (OSCN(-)), thereby potentially limiting the propensity of HOCl to inflict host tissue damage that can lead to inflammatory diseases. However, the efficiency with which SCN(-) captures HOCl in vivo depends on the concentrat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi902089w
更新日期:2010-03-09 00:00:00
abstract::Two successive steps in de novo purine biosynthesis are catalyzed by the enzymes 5-aminoimidazole ribonucleotide (AIR) carboxylase and 4-[(N-succinylamino)carbonyl]-5-aminoimidazole ribonucleotide (SAICAR) synthetase. Amino acid sequence alignments of the proteins from various sources suggested that several unusual di...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00205a030
更新日期:1994-10-04 00:00:00
abstract::The complete amino acid sequence of the major component myoglobin from Amazon River dolphin, Inia geoffrensis, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Three easily separable peptides were obtained by cleaving the protein with cy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00695a018
更新日期:1975-12-02 00:00:00
abstract::The subgel phases of a homologous series of saturated straight-chain diacylphosphatidylcholines with hydrocarbon chains consisting of 10-18 carbon atoms were studied by Fourier-transform infrared spectroscopy. All of these lipids initially form a subgel phase which is spectroscopically similar to that obtained when fu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00486a024
更新日期:1990-08-28 00:00:00
abstract::The PLL(PTE-like lactonase)-group of enzymes within the amidohydrolase superfamily hydrolyze N-acyl-homoserine lactones (AHLs) that are involved in bacterial quorum-sensing pathways. These enzymes possess the (beta/alpha)(8)-barrel fold and serve as attractive templates for in vitro evolution and engineering of quorum...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9004045
更新日期:2009-05-26 00:00:00