Mechanistic studies of the methyltransferase from Clostridium thermoaceticum: origin of the pH dependence of the methyl group transfer from methyltetrahydrofolate to the corrinoid/iron-sulfur protein.

abstract：:Retinal rod and cone cGMP phosphodiesterases (PDE6 family) function as the effector enzyme in the vertebrate visual transduction cascade. The activity of PDE6 catalytic subunits is controlled by the Pgamma-subunits. In addition to the inhibition of cGMP hydrolysis at the catalytic sites, Pgamma is known to stimulate a...

journal_title：Biochemistry

authors： Muradov KG,Granovsky AE,Schey KL,Artemyev NO

更新日期：2002-03-26 00:00:00

abstract：:Isoforms of transforming growth factor beta (TGFbeta) are 25 kDa homodimeric polypeptides that signal by binding and bringing together two related, functionally distinct cell surface receptors designated as TbetaR1 and TbetaR2. Here, we report the solution structure of the 13.8 kDa extracellular domain of human TbetaR...

journal_title：Biochemistry

authors： Deep S,Walker KP 3rd,Shu Z,Hinck AP

更新日期：2003-09-02 00:00:00

abstract：:The rates of inactivation of BF1 adenosinetriphosphatase (BF1-ATPase) from Escherichia coli by 7-chloro-4-nitro-2,1,3-benzoxadiazole, 1-fluoro-2,4-dinitrobenzene, 2,4,6-trinitrobenzenesulfonate, phenylglyoxal, and N,N'-dicyclohexylcarbodiimide have been measured in the presence and absence of various concentrations of...

journal_title：Biochemistry

authors： Ting LP,Wang JH

更新日期：1982-01-19 00:00:00

abstract：:We have studied carbon monoxide (CO) migration and binding in the nonsymbiotic hemoglobins AHb1 and AHb2 of Arabidopsis thaliana using Fourier transform infrared (FTIR) spectroscopy combined with temperature derivative spectroscopy (TDS) at cryogenic temperatures. Both proteins have similar amino acid sequences but di...

journal_title：Biochemistry

authors： Nienhaus K,Dominici P,Astegno A,Abbruzzetti S,Viappiani C,Nienhaus GU

更新日期：2010-09-07 00:00:00

abstract：:To gain insight into the mechanism by which Arg-163 influences oligomerization of alphaA-crystallin, we prepared a series of truncated alphaA-crystallins with or without mutation of the Arg-163 residue. Expression of the proteins was achieved in Escherichia coli BL21 (DE3) pLysS cells, and alphaA-crystallin was purifi...

journal_title：Biochemistry

authors： Rajan S,Chandrashekar R,Aziz A,Abraham EC

更新日期：2006-12-26 00:00:00

abstract：:The prothrombinase complex is comprised of an enzyme, factor Xa, and a cofactor, factor Va, that each bind peripherally to membranes containing phosphatidylserine (PS) and activate the substrate, prothrombin. The mechanism by which the membrane contributes to enhanced catalytic efficacy of prothrombinase is not precis...

journal_title：Biochemistry

authors： Stone MD,Nelsestuen GL

更新日期：2005-03-15 00:00:00

abstract：:NMR spectroscopic experiments were performed to study the structure of synthetic peptides identical to two extracellular regions of the human thrombin receptor. The smaller molecule, comprising 14 amino acids, was biologically active and was equivalent to the "tethered ligand" exposed after cleavage of the receptor by...

journal_title：Biochemistry

authors： Smith KJ,Trayer IP,Grand RJ

更新日期：1994-05-24 00:00:00

abstract：:In order to investigate the changes of protonation or environment of carboxylic residues occurring upon photoreduction of the secondary quinone acceptor (QB) in the reaction center (RC) of the photosynthetic bacteria Rhodobacter sphaeroides 2.4.1., we have performed light-induced Fourier transform infrared (FTIR) spec...

journal_title：Biochemistry

authors： Nabedryk E,Breton J,Hienerwadel R,Fogel C,Mäntele W,Paddock ML,Okamura MY

更新日期：1995-11-14 00:00:00

abstract：:It is known that aconitase from mammalian mitochondria is only partially active as isolated but may be activated by incubation with iron, ascorbate, and a thiol, or with dithionite. It has been suggested that the added Fe in the activation mixture is essential for activation and that it is incorporated in the enzyme [...

journal_title：Biochemistry

authors： Ramsay RR,Dreyer JL,Schloss JV,Jackson RH,Coles CJ,Beinert H,Cleland WW,Singer TP

更新日期：1981-12-22 00:00:00

abstract：:The voltage-gated potassium channel, K(v)1.3, is a novel target for development of immunosuppressants. Using a functional (86)Rb(+) efflux assay, a new class of high-affinity K(v)1.3 inhibitors has been identified. The initial active in this series, 4-phenyl-4-[3-(2-methoxyphenyl)-3-oxo-2-azaprop-1-yl]cyclohexanone (P...

journal_title：Biochemistry

authors： Schmalhofer WA,Bao J,McManus OB,Green B,Matyskiela M,Wunderler D,Bugianesi RM,Felix JP,Hanner M,Linde-Arias AR,Ponte CG,Velasco L,Koo G,Staruch MJ,Miao S,Parsons WH,Rupprecht K,Slaughter RS,Kaczorowski GJ,Garcia ML

更新日期：2002-06-18 00:00:00

abstract：:Tularemia is a severe infectious disease in humans caused by the Gram-negative bacterium Francisella tularensis (Ft). Because of its low infectious dose, high mortality rate, and the threat of its large-scale dissemination in weaponized form, development of vaccines and immunotherapeutics against Ft is essential. Ft l...

journal_title：Biochemistry

authors： Wang Q,Shi X,Leymarie N,Madico G,Sharon J,Costello CE,Zaia J

更新日期：2011-12-20 00:00:00

abstract：:Recent studies implicate hydrogen sulfide (H2S) oxidation as an important aspect of bacterial antibiotic resistance and sulfide homeostasis. The cst operon of the major human pathogen Staphylococcus aureus is induced by exogenous H2S stress and encodes enzymes involved in sulfide oxidation, including a group I flavopr...

journal_title：Biochemistry

authors： Shen J,Peng H,Zhang Y,Trinidad JC,Giedroc DP

更新日期：2016-11-29 00:00:00

abstract：:The structure of the 56-residue B1 immunoglobulin-binding domain from streptococcal protein G has been determined in two different crystal forms. The crystal structures were deduced by molecular replacement, based on the structure of the B2 domain (Brookhaven accession code 1PGX). Final R values are 0.174 and 0.198 fo...

journal_title：Biochemistry

authors： Gallagher T,Alexander P,Bryan P,Gilliland GL

更新日期：1994-04-19 00:00:00

abstract：:Electron-transfer reactions following the formation of P700(+)A1- have been studied in isolated Photosystem I complexes from Synechococcus elongatus between 300 and 5 K by flash absorption spectroscopy. (1) In the range from 300 to 200 K, A1- is reoxidized by electron transfer to the iron-sulfur cluster FX. The rate s...

journal_title：Biochemistry

authors： Schlodder E,Falkenberg K,Gergeleit M,Brettel K

更新日期：1998-06-30 00:00:00

abstract：:The thermal denaturation of the cupredoxin plastocyanin (PC) from spinach has been studied with the aim of improving the understanding of factors involved in the conformational stability of antiparallel beta-sheet proteins. Studies using differential scanning calorimetry have been complemented with nuclear magnetic re...

journal_title：Biochemistry

authors： Sandberg A,Harrison DJ,Karlsson BG

更新日期：2003-09-02 00:00:00

abstract：:The initiation of Escherichia coli chromosomal replication by DnaA protein is strongly influenced by the tight binding of the nucleotides ATP and ADP. Anionic phospholipids in a fluid bilayer promote the conversion of inactive ADP-DnaA protein to replicatively active ATP-DnaA protein in vitro, and thus likely play a k...

journal_title：Biochemistry

authors： Kitchen JL,Li Z,Crooke E

更新日期：1999-05-11 00:00:00

abstract：:Phosphatidylserine (PS) in the plasma membrane of nonactivated human platelets is almost entirely located on the cytoplasmic side. Stimulation of platelets with the Ca2+ ionophore A23187 or combined action of collagen plus thrombin results in a rapid loss of the asymmetric distribution of PS. Also, treatment with the ...

journal_title：Biochemistry

authors： Bevers EM,Tilly RH,Senden JM,Comfurius P,Zwaal RF

更新日期：1989-03-21 00:00:00

abstract：:Comparisons of catalytic mechanisms have not previously been performed for homologous enzymes from hyperthermophilic and mesophilic sources. Here, the beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus was recombinantly produced in Escherichia coli and shown to have biophyscial and biochemical pr...

journal_title：Biochemistry

authors： Bauer MW,Kelly RM

更新日期：1998-12-08 00:00:00

abstract：:Two oxygenated iron-cobalt hybrid hemoglobins (Hbs), (alpha Co-O2 beta Fe-O2)2 and (alpha Fe-O2 beta Co-O2)2, were studied by electron spin echo envelope modulation (ESEEM) spectroscopy in order to measure (i) electron-nuclear hyperfine and nuclear quadrupole coupling to the N epsilon of the proximal histidyl imidazol...

journal_title：Biochemistry

authors： Lee HC,Peisach J,Tsuneshige A,Yonetani T

更新日期：1995-05-23 00:00:00

abstract：:5,6-Dihydrothymidine 5'-triphosphate (DHdTTP) was synthesized by catalytic hydrogenation of thymidine 5'-triphosphate (dTTP). Thymidine glycol 5'-triphosphate (dTTP-GLY) was prepared by bromination of dTTP followed by treatment with Ag2O. The modified nucleotides were extensively purified by anion-exchange high-perfor...

journal_title：Biochemistry

authors： Ide H,Melamede RJ,Wallace SS

更新日期：1987-02-10 00:00:00

abstract：:The cAMP-dependent protein kinase (PKA) controls a large number of cellular functions. One critical PKA substrate in the brain and heart is the L-type Ca(2+) channel Ca(v)1.2, the activity of which is upregulated by PKA. The main PKA phosphorylation site is serine 1928 in the central pore forming alpha(1)1.2 subunit o...

journal_title：Biochemistry

authors： Hall DD,Feekes JA,Arachchige Don AS,Shi M,Hamid J,Chen L,Strack S,Zamponi GW,Horne MC,Hell JW

更新日期：2006-03-14 00:00:00

abstract：:Influenza virus gains access to the cytoplasm of its host cell by means of a fusion event between viral and host cell membrane. Fusion is mediated by the envelope glycoprotein hemagglutinin (HA) and is triggered by low pH. To learn how many hemagglutinin trimers are necessary to cause membrane fusion, we have used two...

journal_title：Biochemistry

authors： Ellens H,Bentz J,Mason D,Zhang F,White JM

更新日期：1990-10-16 00:00:00

abstract：:Many naturally occurring RNA structures contain single mismatches. However, the algorithms currently used to predict RNA structure from sequence rely on a minimal set of data for single mismatches, most of which occur rather infrequently in nature. As a result, several approximations and assumptions are used to predic...

journal_title：Biochemistry

authors： Davis AR,Znosko BM

更新日期：2007-11-20 00:00:00

abstract：:β-Lactamase inhibitors (BLIs) restore the efficacy of otherwise obsolete β-lactams. However, commercially available BLIs are not effective against metallo-β-lactamases (MBLs), which continue to be disseminated globally. One group of the most clinically important MBLs is the VIM family. The discovery of VIM-24, a natur...

journal_title：Biochemistry

authors： Mojica MF,Mahler SG,Bethel CR,Taracila MA,Kosmopoulou M,Papp-Wallace KM,Llarrull LI,Wilson BM,Marshall SH,Wallace CJ,Villegas MV,Harris ME,Vila AJ,Spencer J,Bonomo RA

更新日期：2015-05-26 00:00:00

abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins as well as the in-vitro reconstitution of the first steps of this biosynthesis. We now report experimental evidence supporting the propos...

journal_title：Biochemistry

authors： Mann S,Lombard B,Loew D,Méjean A,Ploux O

更新日期：2011-08-23 00:00:00

abstract：:Base excision repair is a major pathway for the removal of simple lesions in DNA including base damage and base loss (abasic site). Base excision repair requires the coordinated action of several repair and ancillary proteins, the impairment of which can lead to genetic instability. Using a protein-DNA cross-linking a...

journal_title：Biochemistry

authors： Parsons JL,Dianova II,Allinson SL,Dianov GL

更新日期：2005-08-09 00:00:00

abstract：:Human fibroblast interferon binds to a concanavalin A-agarose (Con A-Sepharose) equilibrated with methyl alpha-D-mannopyranoside, or levan; in contrast, it is only partially retarded on a similar column equilibrated with ethylene glycol. Interferon does not bind, however, to a lectin column equilibrated with both meth...

journal_title：Biochemistry

authors： Davey MW,Sulkowski E,Carter WA

更新日期：1976-02-10 00:00:00

abstract：:The human double-stranded RNA- (dsRNA) activated protein kinase (PKR) has a dsRNA-binding domain (dsRBD) that contains two tandem copies of the dsRNA-binding motif (dsRBM). The minimal-length polypeptide required to bind dsRNA contains both dsRBMs, as determined by mobility-shift and filter-binding assays. Mobility-sh...

journal_title：Biochemistry

authors： Bevilacqua PC,Cech TR

更新日期：1996-08-06 00:00:00

abstract：:We have examined the DNA damage produced by reaction of peroxyl radicals with human fibroblast DNA. DNA damage consisted of both strand breaks and base modifications. The extent of strand breaks and base modifications induced as a function of peroxyl radical concentration was determined by quantitation of fragment siz...

journal_title：Biochemistry

authors： Rodriguez H,Valentine MR,Holmquist GP,Akman SA,Termini J

更新日期：1999-12-14 00:00:00

abstract：:The gene encoding for the methionyl aminopeptidase from the hyperthermophilic archaeon Pyrococcus furiosus (PfMetAP-II; EC 3.4.11.18) has been inserted into a pET 27b(+) vector and overexpressed in Escherichia coli. The new expression system resulted in a 5-fold increase in purified enzyme obtained from a 5 L fermento...

journal_title：Biochemistry

authors： Meng L,Ruebush S,D'souza VM,Copik AJ,Tsunasawa S,Holz RC

更新日期：2002-06-11 00:00:00