Cloning of synthetic deoxyribonucleic acid that codes for embryonic cardiac myosin light-chain polypeptide.

abstract：:Monoamine oxidase B (MAO B) is an integral protein of the outer mitochondrial membrane that is involved in the deamination of vasoactive and neuroactive amines. The oxidation of these amine substrates requires the cofactor FAD, which is covalently bound to Cys-397 of human MAO B. Previously, Glu-34 and Tyr-44 of MAO B...

journal_title：Biochemistry

authors： Kirksey TJ,Kwan SW,Abell CW

更新日期：1998-09-01 00:00:00

abstract：:Treatment of human lymphoid cells with methyl methanesulfonate (MMS) results in discontinuities in cellular DNA. Discontinuities can be detected by sedimentation through neutral sucrose gradients after digestion with S1 nuclease on top of the gradient and by benzoylated naphthoylated diethylaminoethylcellulose (BND-ce...

journal_title：Biochemistry

authors： Karran P,Higgins NP,Strauss B

更新日期：1977-10-04 00:00:00

abstract：:Guanidine hydrochloride (GdnHCl) and thermally induced unfolding measurements on the oxidized form of Escherichia coli thioredoxin at pH 7 were combined for the purpose of assessing the functional dependence of unfolding free energy changes on denaturant concentration over an extended GdnHCl concentration range. Conve...

journal_title：Biochemistry

authors： Santoro MM,Bolen DW

更新日期：1992-05-26 00:00:00

abstract：:We have developed a homogeneous time-resolved fluorescence resonance energy transfer (FRET)-based assay that detects the formation of HIV-1 integrase (IN) dimers. The assay utilizes IN monomers that express two different epitope tags that are recognized by their respective antibodies, coupled to distinct fluorophores....

journal_title：Biochemistry

authors： Tsiang M,Jones GS,Hung M,Samuel D,Novikov N,Mukund S,Brendza KM,Niedziela-Majka A,Jin D,Liu X,Mitchell M,Sakowicz R,Geleziunas R

更新日期：2011-03-15 00:00:00

abstract：:Two chimeric receptors were constructed by transposing the coding regions for the putative N-terminal domains of the human calcitonin (hCTR) and glucagon (hGGR) receptors. These receptors were stably expressed as glycosylated proteins with molecular masses of 80 kDa for the calcitonin receptor N-terminus chimera (NtCT...

journal_title：Biochemistry

authors： Stroop SD,Kuestner RE,Serwold TF,Chen L,Moore EE

更新日期：1995-01-24 00:00:00

abstract：:The processes of liposome solubilization and reconstitution were studied by using n-dodecyl octaethylene glycol monoether (C12E8). The solubilization of large unilamellar liposomes prepared by reverse-phase evaporation was systematically investigated by turbidity, 31P nuclear magnetic resonance, and centrifugation exp...

journal_title：Biochemistry

authors： Levy D,Gulik A,Seigneuret M,Rigaud JL

更新日期：1990-10-09 00:00:00

abstract：:DNA photolyase from Escherichia coli contains FAD plus a partially characterized, second chromophore. In vivo, the flavin is fully reduced (FADH2), but oxidation to a stable, blue radical (FADH.) occurs during enzyme isolation. The second chromophore is irreversibly reduced by reaction of the enzyme with sodium borohy...

journal_title：Biochemistry

authors： Jorns MS,Wang B,Jordan SP

更新日期：1987-10-20 00:00:00

abstract：:Elucidation of the molecular mechanism leading to biomembrane fusion is a challenging issue in current biomedical research in view of its involvement in controlling cellular functions and in mediating various important diseases. According to the generally admitted stalk mechanism described for membrane fusion, negativ...

journal_title：Biochemistry

authors： El Kirat K,Dufrêne YF,Lins L,Brasseur R

更新日期：2006-08-01 00:00:00

abstract：:A Rhodobacter sphaeroides bchD (magnesium chelatase) mutant was studied to determine the properties of its photosystem in the absence of bacteriochlorophyll (BChl). Western blots of reaction center H, M, and L (RC H/M/L) proteins from mutant membranes showed levels of 12% RC H, 32% RC L, and 46% RC M relative to those...

journal_title：Biochemistry

authors： Jaschke PR,Beatty JT

更新日期：2007-10-30 00:00:00

abstract：:Cytochrome c binds to cardiolipin (CL) on the inner mitochondrial membrane during the initial stages of apoptosis where it oxidizes CL, promoting its release into the cytoplasm where it initiates apoptosis. Previous work has identified interaction sites on cytochrome c involved in the cytochrome c-CL interaction. The ...

journal_title：Biochemistry

authors： Elmer-Dixon MM,Bowler BE

更新日期：2018-10-02 00:00:00

abstract：:Prion diseases are associated with a conformational switch in the prion protein (PrP) from its normal cellular form (denoted PrP(C)) to a disease-associated "scrapie" form (PrP(Sc)). A number of PrP(Sc)-like conformations can be generated by incubating recombinant PrP(C) at low pH, indicating that protonation of key r...

journal_title：Biochemistry

authors： Hosszu LL,Tattum MH,Jones S,Trevitt CR,Wells MA,Waltho JP,Collinge J,Jackson GS,Clarke AR

更新日期：2010-10-12 00:00:00

abstract：:The transferrins (TFs) are a family of proteins that are widely distributed in vertebrates, where they serve a major role in iron binding and transport. Most TFs are composed of two homologous lobes, the N- and C-lobes, each able to bind a single iron atom. Human serum transferrin (hTF) binds iron in the blood and del...

journal_title：Biochemistry

authors： Halbrooks PJ,He QY,Briggs SK,Everse SJ,Smith VC,MacGillivray RT,Mason AB

更新日期：2003-04-08 00:00:00

abstract：:His216 is a well-conserved residue in pyruvate carboxylases and, on the basis of structures of the enzyme, appears to have a role in the binding of MgATP, forming an interaction with the 3'-hydroxyl group of the ribose ring. Mutation of this residue to asparagine results in a 9-fold increase in the Km for MgATP in its...

journal_title：Biochemistry

authors： Adina-Zada A,Jitrapakdee S,Wallace JC,Attwood PV

更新日期：2014-02-18 00:00:00

abstract：:Extracellular matrix (ECM) proteins, such as collagen and fibronectin, play vital roles in development and maintenance of hard tissue (bone or tooth) and are, consequently, thoroughly investigated for construction of biomimetic scaffolds in combination with calcium phosphate (CaP) material (the major component of hard...

journal_title：Biochemistry

authors： Chowdhury EH,Nagaoka M,Ogiwara K,Zohra FT,Kutsuzawa K,Tada S,Kitamura C,Akaike T

更新日期：2005-09-20 00:00:00

abstract：:For three prokaryotic enzymes of the xanthine oxidase family, namely quinoline 2-oxidoreductase, quinaldine 4-oxidase, and isoquinoline 1-oxidoreductase, the electron transfer centers were investigated by electron paramagnetic resonance. The enzymes are containing a molybdenum-molybdopterin cytosine dinucleotide cofac...

journal_title：Biochemistry

authors： Canne C,Stephan I,Finsterbusch J,Lingens F,Kappl R,Fetzner S,Hüttermann J

更新日期：1997-08-12 00:00:00

abstract：:Primase DnaG is an essential bacterial enzyme that synthesizes short ribonucleotide primers required for chromosomal DNA replication. Inhibitors of DnaG can serve as leads for development of new antibacterials and biochemical probes. We recently developed a nonradioactive in vitro primase-pyrophosphatase assay to iden...

journal_title：Biochemistry

authors： Biswas T,Green KD,Garneau-Tsodikova S,Tsodikov OV

更新日期：2013-10-01 00:00:00

abstract：:In investigating the agonist binding site of the human brain cholecystokininB receptor (CCKBR), we employed the direct protein chemical approach using a photoreactive tritiated analogue of sulfated cholecystokinin octapeptide, which contains the p-benzoylbenzoyl moiety at the N-terminus, followed by purification of th...

journal_title：Biochemistry

authors： Anders J,Blüggel M,Meyer HE,Kühne R,ter Laak AM,Kojro E,Fahrenholz F

更新日期：1999-05-11 00:00:00

abstract：:In animals, the post-translational hydroxylation of hypoxia inducible factor (HIF) is a central mechanism for regulating gene expression in an oxygen-dependent manner. The oxygenase-catalyzed trans-4-prolyl hydroxylation of HIF-alpha increases its affinity for the von Hippel Lindau protein elongin B/C (VCB) ubiquitin ...

journal_title：Biochemistry

authors： Illingworth CJ,Loenarz C,Schofield CJ,Domene C

更新日期：2010-08-17 00:00:00

abstract：:Peptidyl derivatives of diphenyl (alpha-aminoalkyl)phosphonates have been synthesized and are effective and specific inhibitors of serine proteases at low concentrations. Z-PheP(OPh)2 irreversibly reacts with chymotrypsin (kobsd/[I] = 1200 M-1 s-1) and does not react with two elastases. The best inhibitor for most chy...

journal_title：Biochemistry

authors： Oleksyszyn J,Powers JC

更新日期：1991-01-15 00:00:00

abstract：:Homology modeling was used to identify two particular residues, Glu175 and Ala176, in Pseudomonas stutzeri phosphite dehydrogenase (PTDH) as the principal determinants of nicotinamide cofactor (NAD(+) and NADP(+)) specificity. Replacement of these two residues by site-directed mutagenesis with Ala175 and Arg176 both s...

journal_title：Biochemistry

authors： Woodyer R,van der Donk WA,Zhao H

更新日期：2003-10-14 00:00:00

abstract：:Factor X (FX) is one of the major players in the blood coagulation cascade. Upon activation to FXa, it converts prothrombin to thrombin, which in turn converts fibrinogen into fibrin (blood clots). FXa deficiency causes hemostasis defects, such as intracranial bleeding, hemathrosis, and gastrointestinal blood loss. He...

journal_title：Biochemistry

authors： Abdel-Azeim S,Oliva R,Chermak E,De Cristofaro R,Cavallo L

更新日期：2014-11-11 00:00:00

abstract：:HK31 (S565-K595) has previously been shown to encompass the binding domain for plasma prekallikrein (PK) within domain 6 of high molecular weight kininogen (HK). The complementary binding domain for HK within PK is mapped to PK56 (F56-G86), in the apple 1 domain, and to PK266 (K266-C295), in the apple 4 domain. Isothe...

journal_title：Biochemistry

authors： Lin Y,Shenoy SS,Harris RB,Colman RW

更新日期：1996-10-01 00:00:00

abstract：:The zinc metalloenzyme protein farnesyltransferase (FTase) catalyzes the alkylation of a cysteine residue of protein substrates with a 15 carbon farnesyl group. We have developed fluorescence assays to directly measure the affinity of the enzyme for peptide and protein (Ras) substrates. A peptide corresponding to the ...

journal_title：Biochemistry

authors： Hightower KE,Huang CC,Casey PJ,Fierke CA

更新日期：1998-11-03 00:00:00

abstract：:Activation of the protein tyrosine kinase receptors requires the coupling of ligand binding to a change in both the proximity and orientation of the single transmembrane (TM) helices of receptor monomers to allow transphosphorylation of the receptor kinase domain. We make use of peptides corresponding to the TM and ju...

journal_title：Biochemistry

authors： Tamagaki H,Furukawa Y,Yamaguchi R,Hojo H,Aimoto S,Smith SO,Sato T

更新日期：2014-08-05 00:00:00

abstract：:Using our Escherichia coli expression system, we have produced five mutant recombinant (r) hemoglobins (Hbs): r Hb (alpha V96 W), r Hb Presbyterian (beta N108K), r Hb Yoshizuka (beta N108D), r Hb (alpha V96W, beta N108K), and r Hb (alpha V96W, beta N108D). These r Hbs allow us to investigate the effect on the structur...

journal_title：Biochemistry

authors： Tsai CH,Shen TJ,Ho NT,Ho C

更新日期：1999-07-06 00:00:00

abstract：:Diethyl pyrocarbonate inhibited diaphorase activity of ferredoxin-NADP+ oxidoreductase with a second-order rate constant of 2 mM-1 X min-1 at pH 7.0 and 20 degrees C, showing a concomitant increase in absorbance at 242 nm due to formation of carbethoxyhistidyl derivatives. Activity could be restored by hydroxylamine, ...

journal_title：Biochemistry

authors： Carrillo N,Vallejos RH

更新日期：1983-12-06 00:00:00

abstract：:We report nuclear magnetic resonance (NMR) data indicating that the Rieske protein from the cytochrome bc complex of Thermus thermophilus (TtRp) undergoes modest redox-state-dependent and ligand-dependent conformational changes. To test models concerning the mechanism by which TtRp transfers between different sites on...

journal_title：Biochemistry

authors： Hsueh KL,Tonelli M,Cai K,Westler WM,Markley JL

更新日期：2013-04-30 00:00:00

abstract：:We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, with a capability comparable to that se...

journal_title：Biochemistry

authors： Yang Y,Zhang Q,Gao M,Yang X,Huang Z,An J

更新日期：2014-08-05 00:00:00

abstract：:The complete primary structure of the major component myoglobin from the Arctic minke whale, Balaenoptera acutorostrata, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Over 80% of the amino acid sequence was established from the three ...

journal_title：Biochemistry

authors： Lehman LD,Dwulet FE,Bogardt RA Jr,Jones BN,Gurd FR

更新日期：1977-02-22 00:00:00

abstract：:Voltage-dependent L-type calcium (Ca) channels are heteromultimeric proteins that are regulated through phosphorylation by cAMP-dependent protein kinase (PKA). We demonstrated that the beta 2 subunit was a substrate for PKA in intact cardiac myocytes through back-phosphorylation experiments. In addition, a heterologou...

journal_title：Biochemistry

authors： Gerhardstein BL,Puri TS,Chien AJ,Hosey MM

更新日期：1999-08-10 00:00:00