An inhibitor of ribosomal peptidyl transferase using transition-state analogy.

abstract：:Listeriolysin O (LLO) is the most important virulence factor of the intracellular pathogen Listeria monocytogenes. Its main task is to enable escape of bacteria from the phagosomal vacuole into the cytoplasm. LLO belongs to the cholesterol-dependent cytolysin (CDC) family but differs from other members, as it exhibits...

journal_title：Biochemistry

authors： Bavdek A,Gekara NO,Priselac D,Gutiérrez Aguirre I,Darji A,Chakraborty T,Macek P,Lakey JH,Weiss S,Anderluh G

更新日期：2007-04-10 00:00:00

abstract：:The microtubule-associated protein tau, in a hyperphosphorylated form, aggregates into insoluble paired-helical filaments (PHFs) in Alzheimer's disease (AD) and other tauopathies. In AD, there is approximately 8 mol of phosphate per mole of tau distributed among approximately 30 PHF phosphorylation sites as compared t...

journal_title：Biochemistry

authors： Sun Q,Gamblin TC

更新日期：2009-06-30 00:00:00

abstract：:The interaction of insulin with its receptor is complex. Kinetic and equilibrium binding studies suggest coexistence of high- and low-affinity binding sites or negative cooperativity. These phenomena and high-affinity interactions are dependent on the dimeric structure of the receptor. Structure-function studies of in...

journal_title：Biochemistry

authors： Whittaker L,Hao C,Fu W,Whittaker J

更新日期：2008-12-02 00:00:00

abstract：:Orthophosphate and phosphate derivatives including pyrophosphate, hexametaphosphate, ATP, ADP, and inositol hexaphosphate enhance the extent of DNA degradation by iron(II) bleomycin. These phosphate-containing compounds increase both the release of free nucleic base and that of base propenals which are DNA cleavage pr...

journal_title：Biochemistry

authors： Burger RM,Horwitz SB,Peisach J

更新日期：1985-07-02 00:00:00

abstract：:Cysteine dioxygenase (CDO) is a non-heme monoiron enzyme with an unusual posttranslational modification in the proximity of the ferrous iron active site. This modification, a cysteine to tyrosine thioether bond, cross-links two β-strands of the β-barrel. We have investigated its role in catalysis through a combined cr...

journal_title：Biochemistry

authors： Davies CG,Fellner M,Tchesnokov EP,Wilbanks SM,Jameson GN

更新日期：2014-12-23 00:00:00

abstract：:The temperature dependence of the packing (order) and fluidity (microviscosity) of rabbit small, intestinal brush border vesicle membranes and of liposomes made from their extracted lipids has been investigated by using a variety of lipid spin probes. The lipids in the brush border membrane are present essentially as ...

journal_title：Biochemistry

authors： Hauser H,Gains N,Semenza G,Spiess M

更新日期：1982-10-26 00:00:00

abstract：:Peroxynitrite-mediated oxidative chemistry is currently the subject of intense investigation owing to the toxic side effects associated with nitric oxide overproduction. Using direct electron spin resonance spectroscopy (ESR) at 37 degrees C, we observed that in human erythrocytes peroxynitrite induced a long-lived si...

journal_title：Biochemistry

authors： Minetti M,Scorza G,Pietraforte D

更新日期：1999-02-16 00:00:00

abstract：:Abortive transcription initiation can be rate-limiting for promoter escape and therefore represents a barrier to productive gene expression. The mechanism for abortive initiation is unknown, but the amount of abortive transcript is known to vary with the composition of the initial transcribed sequence (ITS). Here, we ...

journal_title：Biochemistry

authors： Skancke J,Bar N,Kuiper M,Hsu LM

更新日期：2015-07-21 00:00:00

abstract：:The interaction of the so-called fusion peptide of the human immunodeficiency virus gp41 envelope glycoprotein with the target cell membrane is believed to trigger the fusion process which allows the entry of the virus into the cell. Many studies on the interaction of the fusion peptide with biological membranes have ...

journal_title：Biochemistry

authors： Buzón V,Padrós E,Cladera J

更新日期：2005-10-11 00:00:00

abstract：:The N-terminus of the major polypeptide component of the light-harvesting complex (LHC) from the brown alga Laminaria saccharina is blocked. Two partial sequences, one near the N-terminus and the other near the C-terminus, have been obtained by chemical cleavage with acetic acid and N-chlorosuccinimide. Four peptides ...

journal_title：Biochemistry

authors： Douady D,Rousseau B,Caron L

更新日期：1994-03-22 00:00:00

abstract：:The amino acid histidine can play a significant role in the structure and function of proteins. Its various functions include enzyme catalysis, metal binding activity, and involvement in cation-π, π-π, salt-bridge, and other types of noncovalent interactions. Although histidine's imidazole nitrogens (Nδ and Nε) are kn...

journal_title：Biochemistry

authors： Krishna Deepak RN,Sankararamakrishnan R

更新日期：2016-07-12 00:00:00

abstract：:Applications of sensitive new technologies, in particular, two-dimensional NMR spectroscopy, have allowed detection of folded structures in short peptide fragments of proteins in aqueous solution under conditions where native proteins fold. These structures are in rapid dynamic exchange with unfolded states. These obs...

journal_title：Biochemistry

authors： Wright PE,Dyson HJ,Lerner RA

更新日期：1988-09-20 00:00:00

abstract：:The steady-state tryptophan fluorescence and time-resolved tryptophan fluorescence of Escherichia coli thioredoxin, calf thymus thioredoxin, and yeast thioredoxin have been studied. In all proteins, the tryptophan residues undergo strong static and dynamic quenching, probably due to charge-transfer interactions with t...

journal_title：Biochemistry

authors： Mérola F,Rigler R,Holmgren A,Brochon JC

更新日期：1989-04-18 00:00:00

abstract：:A high molecular weight inert molecule, poly(ethylene glycol) (PEG), or a soluble protein, ovalbumin, causes parallel bundles of actin filaments in a crystalline-like structure under physiological conditions of ionic compositions and pH. The bundle formation depends on the molecular weight of PEG, and a larger molecul...

journal_title：Biochemistry

authors： Suzuki A,Yamazaki M,Ito T

更新日期：1989-07-25 00:00:00

abstract：:The technique of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) has been applied to a mesophilic (E. coli) dihydrofolate reductase under conditions that allow direct comparison to a thermophilic (B. stearothermophilus) ortholog, Ec-DHFR and Bs-DHFR, respectively. The analysis of hydrogen-deuterium e...

journal_title：Biochemistry

authors： Oyeyemi OA,Sours KM,Lee T,Kohen A,Resing KA,Ahn NG,Klinman JP

更新日期：2011-09-27 00:00:00

abstract：:The tertiary structure of alpha1-acid glycoprotein (AGP) remains unresolved despite its novel function because AGP is a hard target in X-ray and NMR analyses. To elucidate the membrane-induced conformational change of AGP, the vacuum-ultraviolet circular dichroism (VUVCD) spectra of AGP and its constituent sugars were...

journal_title：Biochemistry

authors： Matsuo K,Namatame H,Taniguchi M,Gekko K

更新日期：2009-09-29 00:00:00

abstract：:SecA-lipid interactions are believed to be important for the translocation of precursor proteins across the inner membrane of Escherichia coli [Lill, R., Dowhan, W., & Wickner, W. (1990) Cell 60, 271-280]. SecA insertion into the phospholipid bilayer could a role in this process. We investigated this possibility by st...

journal_title：Biochemistry

authors： Breukink E,Demel RA,de Korte-Kool G,de Kruijff B

更新日期：1992-02-04 00:00:00

abstract：:Resonance energy transfer measurements provide a way to estimate distances between chromophores attached to different sites of macromolecules. There are two unknowns involved in resonance energy transfer measurements, the distance between two chromophores and their relative orientation. When static orientational disor...

journal_title：Biochemistry

authors： Wu P,Brand L

更新日期：1992-09-01 00:00:00

abstract：:PQQ is an exogenous, tricyclic, quino-cofactor for a number of bacterial dehydrogenases. The final step of PQQ formation is catalyzed by PqqC, a cofactorless oxidase. This study focuses on the activation of molecular oxygen in an enzyme active site without metal or cofactor and has identified a specific oxygen binding...

journal_title：Biochemistry

authors： RoseFigura JM,Puehringer S,Schwarzenbacher R,Toyama H,Klinman JP

更新日期：2011-03-08 00:00:00

abstract：:Ca2+-free crystals of sarcoplasmic reticulum Ca2+-ATPase have, up until now, been obtained in the presence of inhibitors such as thapsigargin (TG), bound to the transmembrane region of this protein. Here, we examined the consequences of such binding for the protein. We found that, after TG binding, an active site liga...

journal_title：Biochemistry

authors： Montigny C,Picard M,Lenoir G,Gauron C,Toyoshima C,Champeil P

更新日期：2007-12-25 00:00:00

abstract：:Calcium is required for the substrate binding and for the chemical step of the interfacial catalytic turnover cycle of pancreatic phospholipase A2 (PLA2), but not for the binding of the enzyme to the interface. The role of calcium and other divalent cations (C) is analyzed for the effect on the substrate binding and k...

journal_title：Biochemistry

authors： Yu BZ,Rogers J,Nicol GR,Theopold KH,Seshadri K,Vishweshwara S,Jain MK

更新日期：1998-09-08 00:00:00

abstract：:Steady-state and transient-state analysis of Coprinus cinereus peroxidase, CIP (identical to Arthromyces ramosus peroxidase), was used to characterize the kinetics of the three fundamental steps in heme peroxidase catalysis: compound I (cpd I) formation, cpd I reduction, and compound II (cpd II) reduction. The rate co...

journal_title：Biochemistry

authors： Abelskov AK,Smith AT,Rasmussen CB,Dunford HB,Welinder KG

更新日期：1997-08-05 00:00:00

abstract：:In previous studies [van Wijk, K. J., Bingsmark, S., Aro, E.-M., & Andersson, B. (1995) J. Biol. Chem. 270, 25685-25695; van Wijk, K. J., Andersson, B., & Aro, E.-M. (1996) J. Biol. Chem 271, 9627-9636], we have demonstrated that D1 protein synthesized in isolated chloroplasts and thylakoids is incorporated into the p...

journal_title：Biochemistry

authors： van Wijk KJ,Roobol-Boza M,Kettunen R,Andersson B,Aro EM

更新日期：1997-05-20 00:00:00

abstract：:Novel inhibitors of lupin diadenosine 5',5'''-P(1),P(4)-tetraphosphate (Ap(4)A) hydrolase have been identified by in silico screening of a large virtual chemical library. Compounds were ranked on the basis of a consensus from six scoring functions. From the top 100 ranked compounds six were selected and initially scre...

journal_title：Biochemistry

authors： Branson KM,Mertens HD,Swarbrick JD,Fletcher JI,Kedzierski L,Gayler KR,Gooley PR,Smith BJ

更新日期：2009-08-18 00:00:00

abstract：:We have initiated a broad-based program aimed at understanding the molecular basis of fluorine specificity in enzymatic systems, and in this context, we report crystallographic and biochemical studies on a fluoroacetyl-coenzyme A (CoA) specific thioesterase (FlK) from Streptomyces cattleya. Our data establish that FlK...

journal_title：Biochemistry

authors： Weeks AM,Coyle SM,Jinek M,Doudna JA,Chang MC

更新日期：2010-11-02 00:00:00

abstract：:Active trihydroxynaphthalene reductase (3HNR) is essential for the biosynthesis of fungal melanin by Magnaporthe grisea and is a focus of inhibitor design studies directed toward control of blast disease in rice. Tricyclazole, a preventative fungicide against rice blast, has been previously characterized as inhibiting...

journal_title：Biochemistry

authors： Thompson JE,Basarab GS,Andersson A,Lindqvist Y,Jordan DB

更新日期：1997-02-18 00:00:00

abstract：:Little is known about how a membrane can regulate interactions between transmembrane helices. Here, we show that strong self-interaction of the transmembrane helix of human quiescin sulfhydryl oxidase 2 rests on a motif of conserved amino acids comprising one face of the helix. Atomistic molecular dynamics simulations...

journal_title：Biochemistry

authors： Ried CL,Scharnagl C,Langosch D

更新日期：2016-03-08 00:00:00

abstract：:Synaptotagmin I is a synaptic vesicle protein that contains two C(2) domains and acts as a Ca(2+) sensor in neurotransmitter release. The Ca(2+)-binding properties of the synaptotagmin I C(2)A domain have been well characterized, but those of the C(2)B domain are unclear. The C(2)B domain was previously found to pull ...

journal_title：Biochemistry

authors： Ubach J,Lao Y,Fernandez I,Arac D,Südhof TC,Rizo J

更新日期：2001-05-22 00:00:00

abstract：:The binding of formate ion, a substrate for the peroxidatic reaction of catalase, has been investigated by magnetic resonance techniques. Comparative studies of formate binding to ferric myoglobin have also been performed. The nuclear magnetic relaxation (NMR) rate of formate and water protons is enhanced by the prese...

journal_title：Biochemistry

authors： Hershberg RD,Chance B

更新日期：1975-08-26 00:00:00

abstract：:Apo horse spleen ferritin (apo HoSF) was reconstituted to various core sizes (100-3500 Fe3+/HoSF) by depositing Fe(OH)3 within the hollow HoSF interior by air oxidation of Fe2+. Fe2+ and phosphate (Pi) were then added anaerobically at a 1:4 ratio, and both Fe2+ and Pi were incorporated into the HoSF cores. The resulti...

journal_title：Biochemistry

authors： Johnson JL,Cannon M,Watt RK,Frankel RB,Watt GD

更新日期：1999-05-18 00:00:00