Abstract:
:We have obtained a quantitative description of villin-nucleated actin polymerization in physiological salt by determining the concentrations of free villin (V), villin-actin monomer (VA), villin-actin dimer (VA2), and villin-actin oligomer (VAn). Over a range of actin-villin ratios from 0.1 to 20 we determined the concentration of actin-bound villin by measuring the low-intensity pyrenylactin fluorescence of the two terminal actins in each villin-actin polymer. (To this end we first showed that each villin-actin oligomer and polymer contains two low-intensity pyrenylactin molecules.) We determined the concentration of free villin using a calibrated cutting activity assay. The pattern of increase in bound villin together with the pattern of increase in high-intensity pyrenylactin fluorescence with increasing G-actin concentration indicated, first, that villin-actin monomers were not formed at detectable levels even at a 12-fold villin excess over actin. Second, there was no stoichiometric villin-actin dimer formation at actin-villin ratios of 2. Instead there was an equilibrium between free villin, VA2, and VAn. Defining K1 = [VA]/[V][A] and K2 = [VA2]/[VA][A], a good fit of the data was obtained with K1 much less than K2 and a value of K1K2 = Kv = 10(12)-10(13) M-2 = [VA2]/[V][A]2, i.e., 1/Kv1/2 = (0.3-1) X 10(-6) M. We have assumed here that the monomer binding constant of VA2 to form VA3 was equal to the monomer binding constant of pointed filament ends, K infinity = 1/c infinity, obtained as described below.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Weber A,Northrop J,Bishop MF,Ferrone FA,Mooseker MSdoi
10.1021/bi00383a019subject
Has Abstractpub_date
1987-05-05 00:00:00pages
2528-36issue
9eissn
0006-2960issn
1520-4995journal_volume
26pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Activator of G protein signaling 3 (AGS3) activates the Gbetagamma mating pathway in yeast in a manner that is independent of heptahelical receptors. It competes with Gbetagamma subunits to bind GDP-bound Gi/o(alpha) subunits via four repeated G protein regulatory (GPR) domains in the carboxyl-terminal half of the mol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034561p
更新日期:2003-07-08 00:00:00
abstract::The crystal structure of the ternary complex citrate synthase-oxaloacetate-carboxymethyl coenzyme A has been solved to a resolution of 1.9 A and refined to a conventional crystallographic R factor of 0.185. The structure resembles a proposed transition state of the condensation reaction and suggests that the condensat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:
更新日期:1990-03-06 00:00:00
abstract::Phospholipids containing a saturated fatty acid in the primary position and an unsaturated fatty acid in the secondary position are a major structural part of biological membranes. The mixed-chain hydrophobic core of the membranes is the diacylglycerol part. To better understand the core properties of membranes we hav...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00051a015
更新日期:1995-12-26 00:00:00
abstract::The solution conformation of the type I collagen alpha-1 chain N-telopeptide has been studied by CD and 1H NMR spectroscopy at 600 MHz in CD3OH/H2O (60/40 v/v) and H2O solutions. The 19 amino acids form the N-terminal end of the alpha-1 polypeptide chain. By the combined application of several two-dimensional, phase-s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00446a006
更新日期:1989-10-03 00:00:00
abstract::The origin of the "S3" EPR signal from calcium-depleted photosystem 2 samples has been investigated. This signal is observed after freezing samples under illumination and has been assigned to an interaction between the manganese cluster and an oxidized histidine radical [Boussac et al. (1990) Nature 347; 303-306]. In ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00134a005
更新日期:1992-05-19 00:00:00
abstract::Over 155 mutations within the V2 vasopressin receptor (AVPR2) gene are responsible for nephrogenic diabetes insipidus (NDI). The expression and subcellular distribution of four of these was investigated in transfected cells. These include a point mutation in the seventh transmembrane domain (S315R), a frameshift mutat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002699r
更新日期:2001-06-12 00:00:00
abstract::TP40 is a chimeric protein containing transforming growth factor alpha (TGF-alpha) at the N-terminus and a derivative of a 40,000-Da segment (PE40 delta cys) of Pseudomonas exotoxin (PE). PE40 delta cys contains domains Ib, II, and III of PE in which the cysteines are mutated to alanines. The rationale for inclusion o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00064a037
更新日期:1993-04-06 00:00:00
abstract::The Caenorhabditis elegans Werner syndrome protein, WRN-1, a member of the RecQ helicase family, has a 3'-5' DNA helicase activity. Worms with defective wrn-1 exhibit premature aging phenotypes and an increased level of genome instability. In response to DNA damage, WRN-1 participates in the initial stages of checkpoi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200791p
更新日期:2012-02-21 00:00:00
abstract::The covalent modification of chicken liver fatty acid synthase by 4-(1-pyrenyl)butyryl-CoA (PBA-CoA), a fluorescent analogue of acetyl- and malonyl-CoA, has been studied. The binding isotherms and the kinetics of inactivation suggest 2 mol of PBA-CoA/mol of enzyme is specifically incorporated into the enzyme. Two clas...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00281a032
更新日期:1983-06-07 00:00:00
abstract::Voltage-dependent anion channel 1 (VDAC1) situated in the outer mitochondrial membrane regulates the transfer of various metabolites and is a key player in mitochondria-mediated apoptosis. Although many small chemicals that modulate the functions of VDAC1 have been reported to date, most, if not all, of them cannot be...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01209
更新日期:2019-02-26 00:00:00
abstract::The structure and folding of a novel human insulin mutant, [Thr(B27) --> Pro, Pro(B28) --> Thr]insulin (PT insulin), in aqueous solution and in mixtures of water and 2,2,2-trifluoroethanol (TFE) have been studied by NMR spectroscopy. It was found that PT insulin has a highly flexible structure in pure water and is pre...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0108150
更新日期:2001-09-04 00:00:00
abstract::The human translin protein binds a variety of sequences (chromosomal breakpoint consensus sequences, their sequence variants, as well as nonbreakpoint sequences such as simple AT and GC repeats) at nanomolar protein concentration when short single strands ( approximately 20-30mers) are used as DNA targets. The protein...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026378m
更新日期:2002-12-24 00:00:00
abstract::The level of albumin mRNA in the normal Buffalo rat liver and Morris hepatoma 7777 was compared by molecular hybridization with albumin complementary DNA (cDNA) and translational assays. Albumin mRNA was found to be 10% of the total rat liver poly(A)-containing RNA population but reduced approximately fourfold in the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00608a028
更新日期:1978-07-25 00:00:00
abstract::Apo horse spleen ferritin (apo HoSF) was reconstituted to various core sizes (100-3500 Fe3+/HoSF) by depositing Fe(OH)3 within the hollow HoSF interior by air oxidation of Fe2+. Fe2+ and phosphate (Pi) were then added anaerobically at a 1:4 ratio, and both Fe2+ and Pi were incorporated into the HoSF cores. The resulti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982727u
更新日期:1999-05-18 00:00:00
abstract::Base excision repair (BER) is the major corrective pathway for most spontaneous, oxidative, and alkylation DNA base and sugar damage. X-ray cross-complementing 1 (XRCC1) has been suggested to function at nearly every step of this repair process, primarily through direct protein-protein interactions. Using whole cell e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051161o
更新日期:2005-11-01 00:00:00
abstract::In an effort to gain greater insight into the molecular mechanism of the electron-transfer reactions of cytochrome b(5), the bovine cytochrome b(5)-horse cytochrome c complex has been investigated by high-resolution multidimensional NMR spectroscopy using (13)C, (15)N-labeled cytochrome b(5) expressed from a synthetic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001129o
更新日期:2000-11-21 00:00:00
abstract::We have used 2D 1H NMR to determine the structure of glucagon-like peptide-1-(7-36) amide bound to a dodecylphosphocholine micelle. In this membranelike environment, the peptide hormone is shown to have a structure similar to that observed for glucagon. It consists of an N-terminal random coil segment (residues 1-7), ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00178a009
更新日期:1994-03-29 00:00:00
abstract::In type 2 diabetics, the hormone amylin misfolds into amyloid plaques implicated in the destruction of the pancreatic β-cells that make insulin and amylin. The aggregative misfolding of amylin is pH-dependent, and exposure of the hormone to acidic and basic environments could be physiologically important. Amylin has t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401164k
更新日期:2014-01-21 00:00:00
abstract::Solution scattering studies were performed on a ligand-binding domain (S1S2) of a glutamate receptor ion channel (GluR) in order to study GluR-binding and signal-transduction mechanisms. The core of the ligand-binding domain is homologous to prokaryotic periplasmic binding proteins (PBP), whose binding mechanism invol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982928y
更新日期:1999-08-24 00:00:00
abstract::The complex kinetic behavior commonly observed in protein folding studies suggests that a heterogeneous population of molecules exists in solution and that a number of discrete steps are involved in the conversion of unfolded molecules to the fully native form. A central issue in protein folding is whether any of thes...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9700476
更新日期:1997-05-13 00:00:00
abstract::The vanadate cofactor in vanadium chloroperoxidase has been studied using UV-VIS absorption spectroscopy. A band is present in the near-UV that is red-shifted as compared to free vanadate and shifts in both position and intensity upon change in pH. Mutation of vanadate binding residues has a clear effect on the spectr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9921790
更新日期:2000-02-08 00:00:00
abstract::Purified subunits of intermediate filaments obtained from a variety of tissues and cell types contain O-phosphoserine and, in some cases, smaller amounts of O-phosphothreonine. The O-phosphoserine content was estimated by reaction of performic acid oxidized subunits with methylamine in NaOH. Decamin of BHK-21 and CHO ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00530a030
更新日期:1982-01-05 00:00:00
abstract::Nephrocalcin (NC), a calcium-binding glycoprotein of 14,000 molecular weight as a monomer, is known to inhibit the growth of calcium oxalate monohydrate (COM) crystals in renal tubules. We have isolated NC from bovine kidney tissue and purified into four isoforms, fractions A-D. NC-A and NC-B strongly inhibit the grow...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961200j
更新日期:1996-11-26 00:00:00
abstract::To establish the state of protonation of quinonoid species formed nonenzymically from pyridoxal phosphate (PLP) and diethyl aminomalonate, we have studied absorption spectra of the rapidly established steady-state mixture of species. We have evaluated the formation constant and the spectrum of the mixture of Schiff ba...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00413a050
更新日期:1988-06-28 00:00:00
abstract::The Radical SAM (RS) enzyme PqqE catalyzes the first step in the biosynthesis of the bacterial cofactor pyrroloquinoline quinone, forming a new carbon-carbon bond between two side chains within the ribosomally synthesized peptide substrate PqqA. In addition to the active site RS 4Fe-4S cluster, PqqE is predicted to ha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01097
更新日期:2018-02-27 00:00:00
abstract::X-ray absorption spectra are reported for the multi-Cu oxidase Rhus vernicifera laccase in oxidized and fully reduced forms and for laccase from which the type 2 Cu has been depleted (T2D). The structure of the Cu K edge for both preparations shows the presence of CuII and CuI in the oxidized and reduced states, respe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00310a008
更新日期:1984-07-17 00:00:00
abstract::The C-terminal domain (CT) of apolipoprotein E (apoE), a critical protein involved in cholesterol transport in the plasma and brain, plays an important role in high-affinity lipoprotein binding. Although high-resolution structural information is available for the N-terminal domain of apoE, the structural organization ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901902e
更新日期:2010-03-02 00:00:00
abstract::kappa-Bungarotoxin (kappa-bgt) is a 66-residue peptide originally purified from snake venom that acts as an antagonist at certain acetylcholine receptors. It is one of four homologous kappa-neurotoxins that are distinguished from the structurally related alpha-neurotoxins by their ability to block the alpha 3-subunit-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00248a004
更新日期:1994-11-08 00:00:00
abstract::NLRP4 is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family of cytosolic receptors and a member of an inflammation signaling cascade. Here, we present the crystal structure of the NLRP4 pyrin domain (PYD) at 2.3 Å resolution. The NLRP4 PYD is a member of the death domain (DD) superfamily ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3007059
更新日期:2012-09-18 00:00:00
abstract::The binding of the inhibitor 8-nitroxanthine to urate oxidase has been investigated by Raman and UV-visible absorption spectroscopy. The absorption maximum of 8-nitroxanthine shifts from 380 to 400 nm upon binding to the enzyme, demonstrating that the electronic structure of the ligand is perturbed. It has been propos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0507837
更新日期:2005-08-30 00:00:00