当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Glycolipid Biosurfactants Activate, Dimerize, and Stabilize Thermomyces lanuginosus Lipase in a pH-Dependent Fashion.

Glycolipid Biosurfactants Activate, Dimerize, and Stabilize Thermomyces lanuginosus Lipase in a pH-Dependent Fashion.

Abstract：

:We present a study of the interactions between the lipase from Thermomyces lanuginosus (TlL) and the two microbially produced biosurfactants (BSs), rhamnolipid (RL) and sophorolipid (SL). Both RL and SL are glycolipids; however, RL is anionic, while SL is a mixture of anionic and non-ionic species. We investigate the interactions of RL and SL with TlL at pH 6 and 8 and observe different effects at the two pH values. At pH 8, neither RL nor SL had any major effect on TlL stability or activity. At pH 6, in contrast, both surfactants increase TlL's thermal stability and fluorescence and activity measurements indicate interfacial activation of TlL, resulting in 3- and 6-fold improved activity in SL and RL, respectively. Nevertheless, isothermal titration calorimetry reveals binding of only a few BS molecules per lipase. Size-exclusion chromatography and small-angle X-ray scattering suggest formation of TlL dimers with binding of small amounts of either RL or SL at the dimeric interface, forming an elongated complex. We conclude that RL and SL are compatible with TlL and constitute promising green alternatives to traditional surfactants.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Madsen JK,Kaspersen JD,Andersen CB,Nedergaard Pedersen J,Andersen KK,Pedersen JS,Otzen DE

doi

10.1021/acs.biochem.7b00420

subject

Has Abstract

pub_date

2017-08-15 00:00:00

pages

4256-4268

issue

32

eissn

0006-2960

issn

1520-4995

journal_volume

56

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全

Functional reconstitution of the gamma-aminobutyric acid transporter from synaptic vesicles using artificial ion gradients.
abstract：:The gamma-aminobutyric acid transporter of rat brain synaptic vesicles was reconstituted in proteoliposomes, and its activity was studied in response to artificially created membrane potentials or proton gradients. Changes of the membrane potential were monitored using the dyes oxonol VI and 3,3'-diisopropylthiodicarb...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi00115a009

authors： Hell JW,Edelmann L,Hartinger J,Jahn R

更新日期：1991-12-24 00:00:00
A fluorescent pentameric thiophene derivative detects in vitro-formed prefibrillar protein aggregates.
abstract：:Protein aggregation is associated with a wide range of diseases, and molecular probes that are able to detect a diversity of misfolded protein assemblies are of great importance. The identification of prefibrillar states preceding the formation of well-defined amyloid fibrils is of particular interest both because of ...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi100922r

authors： Hammarström P,Simon R,Nyström S,Konradsson P,Aslund A,Nilsson KP

更新日期：2010-08-17 00:00:00
An essential role for the extracellular domain of the Na,K-ATPase beta-subunit in cation occlusion.
abstract：:The role of the Na,K-ATPase beta-subunit in stabilization of ion-binding sites has been investigated. Treatment of the purified renal Na,K-ATPase with 0.25 M DTT at 40 degrees C for 1 h resulted in 50% loss of Rb occlusion, which correlates with partial reduction of S-S bridges in the extracellular portion of the beta...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi00077a029

authors： Lutsenko S,Kaplan JH

更新日期：1993-07-06 00:00:00
A novel pentasaccharide sequence GlcA(3-sulfate)(beta1-3)GalNAc(4-sulfate)(beta1-4)(Fuc alpha1-3)GlcA(beta1-3)GalNAc(4-sulfate) in the oligosaccharides isolated from king crab cartilage chondroitin sulfate K and its differential susceptibility to chondroi
abstract：:Novel sulfated tetrasaccharide structures containing 3-O-sulfated GlcA were isolated recently from king crab cartilage chondroitin sulfate K [Sugahara, K., Tanaka, Y., Yamada, S., Seno, N., Kitagawa, H., Haslam, S. M., Morris, H. R., & Dell, A. (1996) J. Biol. Chem. 271, 26745-26754]. In this study, we prepared a seri...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi962740j

authors： Kitagawa H,Tanaka Y,Yamada S,Seno N,Haslam SM,Morris HR,Dell A,Sugahara K

更新日期：1997-04-01 00:00:00
Two-step internalization of Ca2+ from a single E approximately P.Ca2 species by the Ca2+-ATPase.
abstract：:Phosphorylation by ATP of E.*Ca2 (sarcoplasmic reticulum vesicles (SRV) with bound 45Ca2+) during 5-10 ms leads to the occlusion of 2 *Ca2+/EPtot [quench by ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) alone] in both "empty" (10 microM free Ca2+in) or "loaded" SRV (20-40 mM free Ca2+in)...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi00408a041

authors： Khananshvili D,Jencks WP

更新日期：1988-04-19 00:00:00
Nepsilon-acetyllysine transfer ribonucleic acid: a biologically active analogue of aminoacyl transfer ribonucleic acids.
abstract：:Unfractionated Escherichia coli tRNA has been aminoacylated with lysine and preferentially acetylated at the epsilon-amino nitrogen of lysine by reaction with N-acetoxysuccinimide. After treatment with peptidyl-tRNA hydrolase, 90% of the aminoacylated tRNA molecules were Nepsilon-acetyl-Lys-tRNA. Post-ribosomal supern...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi00648a018

authors： Johnson AE,Woodward WR,Herbert E,Menninger JR

更新日期：1976-02-10 00:00:00
DNA minor groove adducts formed by a platinum-acridine conjugate inhibit association of tata-binding protein with its cognate sequence.
abstract：:PT-ACRAMTU ([PtCl(en)(ACRAMTU-S)](NO(3))(2), en = ethane-1,2-diamine, ACRAMTU = 1-[2-(acridin-9-ylamino)ethyl]-1,3-dimethylthiourea) is a cytotoxic platinum-acridine conjugate previously shown to form adducts with the N3 endocyclic nitrogen of adenine in the DNA minor groove. This unusual observation and our prior det...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi050745n

authors： Budiman ME,Bierbach U,Alexander RW

更新日期：2005-08-23 00:00:00
Kinetic mechanism of Escherichia coli asparagine synthetase B.
abstract：:Escherichia coli asparagine synthetase B (AS-B) catalyzes the synthesis of asparagine from aspartate, glutamine, and ATP. A combination of kinetic, isotopic-labeling, and stoichiometry studies have been performed to define the nature of nitrogen transfer mediated by AS-B. The results of initial rate studies were consi...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi981058h

authors： Boehlein SK,Stewart JD,Walworth ES,Thirumoorthy R,Richards NG,Schuster SM

更新日期：1998-09-22 00:00:00
Influence of flanking sequence context on the mutagenicity of acetylaminofluorene-derived DNA adducts in mammalian cells.
abstract：:Site-specifically modified oligodeoxynucleotides were used to explore the influence of neighboring base sequence context on the mutagenic potential of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-AF) in mammalian cells. Oligodeoxynucleotides ((5)(')TCCTCCTNXNCTCT...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi0027581

authors： Shibutani S,Suzuki N,Tan X,Johnson F,Grollman AP

更新日期：2001-03-27 00:00:00
Biostructural chemistry of magnesium ion: characterization of the weak binding sites on tRNA(Phe)(yeast). Implications for conformational change and activity.
abstract：:The thermodynamics and kinetics of magnesium binding to tRNA(Phe)(yeast) have been studied directly by 25Mg NMR. In 0.17 M Na+(aq), tRNA(Phe) exists in its native conformation and the number of strong binding sites (Ka greater than or equal to 10(4)) was estimated to be 3-4 by titration experiments, in agreement with ...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi00477a021

authors： Reid SS,Cowan JA

更新日期：1990-06-26 00:00:00
Expression of Vibrio harveyi acyl-ACP synthetase allows efficient entry of exogenous fatty acids into the Escherichia coli fatty acid and lipid A synthetic pathways.
abstract：:Although the Escherichia coli fatty acid synthesis (FAS) pathway is the best studied type II fatty acid synthesis system, a major experimental limitation has been the inability to feed intermediates into the pathway in vivo because exogenously supplied free fatty acids are not efficiently converted to the acyl-acyl ca...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi901890a

authors： Jiang Y,Morgan-Kiss RM,Campbell JW,Chan CH,Cronan JE

更新日期：2010-02-02 00:00:00
Synthetic charybdotoxin-iberiotoxin chimeric peptides define toxin binding sites on calcium-activated and voltage-dependent potassium channels.
abstract：:Charybdotoxin (ChTX) and iberiotoxin (IbTX) are highly charged peptidyl toxins which exhibit 68% sequence identity and share a similar three-dimensional structure. Despite these structural similarities, IbTX and ChTX differ in their selectivity for two types of potassium channels; large conductance calcium-activated p...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi00060a030

authors： Giangiacomo KM,Sugg EE,Garcia-Calvo M,Leonard RJ,McManus OB,Kaczorowski GJ,Garcia ML

更新日期：1993-03-09 00:00:00
Characterization of the interaction between androgen receptor and a new transcriptional inhibitor, SHP.
abstract：:SHP (short heterodimer partner) is an orphan nuclear receptor, first described for its interaction with nuclear receptors. This study explores a new way of inhibiting the androgen-signaling pathway. We demonstrated that SHP inhibited up to 97% of AR-induced activity. Characterization of AR/SHP interaction provided evi...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi011384o

authors： Gobinet J,Auzou G,Nicolas JC,Sultan C,Jalaguier S

更新日期：2001-12-18 00:00:00
The two Caenorhabditis elegans actin-depolymerizing factor/cofilin proteins differently enhance actin filament severing and depolymerization.
abstract：:Actin-depolymerizing factor (ADF)/cofilin enhances the turnover of actin filaments by two separable activities: filament severing and pointed-end depolymerization. Multicellular organisms express multiple ADF/cofilin isoforms in a tissue-specific manner, and the vertebrate proteins are grouped into ADFs and cofilins o...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi050933d

authors： Yamashiro S,Mohri K,Ono S

更新日期：2005-11-01 00:00:00
Inhibitor and NAD+ binding to poly(ADP-ribose) polymerase as derived from crystal structures and homology modeling.
abstract：:Inhibitors of poly(ADP-ribose) polymerase (PARP, EC 2.4.2.30) are of clinical interest because they have potential for improving radiation therapy and chemotherapy of cancer. The refined binding structures of four such inhibitors are reported together with the refined structure of the unligated catalytic fragment of t...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi972383s

authors： Ruf A,de Murcia G,Schulz GE

更新日期：1998-03-17 00:00:00
In Rhodobacter sphaeroides reaction centers, mutation of proline L209 to aromatic residues in the vicinity of a water channel alters the dynamic coupling between electron and proton transfer processes.
abstract：:The X-ray crystallographic structure of the photosynthetic reaction center from Rhodobacter sphaeroides obtained at high resolution has revealed a number of internal water molecules (Ermler, U., Fritzsch, G., Buchanan, S. K., and Michel, H. (1994) Structure 2, 925-936; Stowell, M. H. B., McPhillips, T. M., Rees, D. C....

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi990192e

authors： Tandori J,Sebban P,Michel H,Baciou L

更新日期：1999-10-05 00:00:00
Characterization of a K+-induced conformational switch in a human telomeric DNA oligonucleotide using 2-aminopurine fluorescence.
abstract：:Human telomeric DNA consists of tandem repeats of the DNA sequence d(GGGTTA). Oligodeoxynucleotide telomere models such as d[A(GGGTTA)(3)GGG] (Tel22) fold in a cation-dependent manner into quadruplex structures consisting of stacked G-quartets linked by d(TTA) loops. NMR has shown that in Na(+) solutions Tel22 forms a...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi901357r

authors： Gray RD,Petraccone L,Trent JO,Chaires JB

更新日期：2010-01-12 00:00:00
Mechanistic inferences from the binding of ligands to LpxC, a metal-dependent deacetylase.
abstract：:The metal-dependent deacetylase LpxC catalyzes the first committed step of lipid A biosynthesis in Gram-negative bacteria. Accordingly, LpxC is an attractive target for the development of inhibitors that may serve as potential new antibiotics for the treatment of Gram-negative bacterial infections. Here, we report the...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi060823m

authors： Gennadios HA,Whittington DA,Li X,Fierke CA,Christianson DW

更新日期：2006-07-04 00:00:00
Electrophysiology of respiratory chain complexes and the ADP-ATP exchanger in native mitochondrial membranes.
abstract：:Transport of protons and solutes across mitochondrial membranes is essential for many physiological processes. However, neither the proton-pumping respiratory chain complexes nor the mitochondrial secondary active solute transport proteins have been characterized electrophysiologically in their native environment. In ...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi1011755

authors： Watzke N,Diekert K,Obrdlik P

更新日期：2010-12-07 00:00:00
Initiation, elongation, and processivity of carboxyl-terminal mutants of T7 RNA polymerase.
abstract：:Bacteriophage T7 RNA polymerase is a single-subunit enzyme which has a C-terminal amino acid sequence of Phe-Ala-Phe-Ala883 (FAFA883). Closely related hydrophobic sequences are present at the C termini of seven other single-subunit RNA polymerases, including the mitochondrial RNA polymerase. Mutations at any of the fo...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi962397i

authors： Gardner LP,Mookhtiar KA,Coleman JE

更新日期：1997-03-11 00:00:00
Escherichia coli glycerol kinase: role of a tetramer interface in regulation by fructose 1,6-bisphosphate and phosphotransferase system regulatory protein IIIglc.
abstract：:Escherichia coli glycerol kinase (EC 2.7.1.30; ATP:glycerol 3-phosphotransferase) is a key element in a signal transduction pathway that couples expression of genes required for glycerol metabolism to the relative availability of glycerol and glucose. Its catalytic activity is inhibited by protein-protein interactions...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi00199a040

authors： Liu WZ,Faber R,Feese M,Remington SJ,Pettigrew DW

更新日期：1994-08-23 00:00:00
Isolation of brain endopeptidases: influence of size and sequence of substrates structurally related to bradykinin.
abstract：:Two thiol-activated endopeptidases with pH optima near pH 7.5 were isolated from the supernatant fraction of rabbit brain homogenates by DEAE-cellulose chromatography, gel filtration and isoelectrofocusing. Peptide bond hydrolysis was measured quantitatively by ion-exchange chromatography with an amino acid analyzer. ...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi00654a026

authors： Oliveira EB,Martins AR,Camargo AC

更新日期：1976-05-04 00:00:00
2D NMR studies and 3D structure of the parallel-stranded duplex oligonucleotide Acrm5-alpha-d(TCTAAACTC)-beta-d(AGATTTGAG) via complete relaxation matrix analysis of the NOE effects and molecular mechanics calculations.
abstract：:The three-dimensional structure of the duplex formed by the association of the unnatural oligonucleotide alpha-d(TCTAAACTC) covalently linked to an acridine derivative (m5Acr) with its natural and parallel complementary sequence beta-d(AGATTTGAG) was investigated by nuclear magnetic resonance spectroscopy and constrai...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi00472a031

authors： Guesnet JL,Vovelle F,Thuong NT,Lancelot G

更新日期：1990-05-22 00:00:00
2-Hydroxy-2'-deoxyadenosine 5'-triphosphate enhances A.T --> C.G mutations caused by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate by suppressing its degradation upon replication in a HeLa extract.
abstract：:The coexistence effects of multiple kinds of oxidized deoxyribonucleotides were examined using an SV40 origin-dependent in vitro replication system with a HeLa extract. Oxidized dGTP and dATP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP) and 2-hydroxy-2'-deoxyadenosine 5'-triphosphate (2-OH-dATP), were used...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi062117r

authors： Satou K,Kasai H,Masutani C,Hanaoka F,Harashima H,Kamiya H

更新日期：2007-06-05 00:00:00
Electrophoretic mobilities and diffusion coefficients of hemoglobin at high pH.
abstract：:Diffusion studies by photon correlation of scattered laser light confirm the dissociation of the tetrameric form of human carboxyhemoglobin to dimers above pH 10 and provide new estimates of the subunit dissociation equilibrium constants in this pH range. Electrophoretic light-scattering experiments under the same con...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi00616a014

authors： Haas DD,Ware BR

更新日期：1978-11-14 00:00:00
A new N-terminal recognition domain in caveolin-1 interacts with sterol carrier protein-2 (SCP-2).
abstract：:Although plasma membrane domains, such as caveolae, provide an organizing principle for signaling pathways and cholesterol homeostasis in the cell, relatively little is known regarding specific mechanisms, whereby intracellular lipid-binding proteins are targeted to caveolae. Therefore, the interaction between caveoli...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi7002636

authors： Parr RD,Martin GG,Hostetler HA,Schroeder ME,Mir KD,Kier AB,Ball JM,Schroeder F

更新日期：2007-07-17 00:00:00
Intrinsic Disorder to Order Transitions in the Scaffold Phosphoprotein P from the Respiratory Syncytial Virus RNA Polymerase Complex.
abstract：:Intrinsic disorder is at the center of biochemical regulation and is particularly overrepresented among the often multifunctional viral proteins. Replication and transcription of the respiratory syncytial virus (RSV) relies on a RNA polymerase complex with a phosphoprotein cofactor P as the structural scaffold, which ...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/acs.biochem.5b01332

authors： Noval MG,Esperante SA,Molina IG,Chemes LB,Prat-Gay Gd

更新日期：2016-03-15 00:00:00
A novel DNA topoisomerase I inhibitor with different mechanism from camptothecin induces G2/M phase cell cycle arrest to K562 cells.
abstract：:DNA topoisomerase I (Top1) is an essential nuclear enzyme and a validated target for anticancer agent screening. In a previous study, we found that indolizinoquinoline-5,12-dione derivatives show significant biological activity against several human cancer cell lines. To understand their mechanism of inhibition of can...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi1009419

authors： Wu N,Wu XW,Agama K,Pommier Y,Du J,Li D,Gu LQ,Huang ZS,An LK

更新日期：2010-11-30 00:00:00
Molecular organization in striated domains induced by transmembrane alpha-helical peptides in dipalmitoyl phosphatidylcholine bilayers.
abstract：:Transmembrane (TM) alpha-helical peptides with neutral flanking residues such as tryptophan form highly ordered striated domains when incorporated in gel-state 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayers and inspected by atomic force microscopy (AFM) (1). In this study, we analyze the molecular organiz...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi048047a

authors： Sparr E,Ganchev DN,Snel MM,Ridder AN,Kroon-Batenburg LM,Chupin V,Rijkers DT,Killian JA,de Kruijff B

更新日期：2005-01-11 00:00:00
Nonphotochemical quenching of excitation energy in photosystem II. A picosecond time-resolved study of the low yield of chlorophyll a fluorescence induced by single-turnover flash in isolated spinach thylakoids.
abstract：:Chlorophyll a fluorescence emission is widely used as a noninvasive measure of a number of parameters related to photosynthetic efficiency in oxygenic photosynthetic organisms. The most important component for the estimation of photochemistry is the relative increase in fluorescence yield between dark-adapted samples ...

journal_title：Biochemistry

pub_type： 杂志文章

doi：10.1021/bi9806854

authors： Vasil'ev S,Bruce D

更新日期：1998-08-04 00:00:00