Electrophoretic mobilities and diffusion coefficients of hemoglobin at high pH.

abstract：:A high-molecular-weight glycoprotein has been purified from the cervical mucus of the bonnet monkey (Macaca radiata). The glycoprotein was shown to be homogeneous by electrophoresis, sedimentation equilibrium, and N-terminal group determination, and to contain 19% protein, 19% D-galactose, 18% N-acetyl-D-galactosamine...

journal_title：Biochemistry

authors： Hatcher VB,Schwarzmann GO,Jeanloz RW,McArthur JW

更新日期：1977-04-05 00:00:00

abstract：:Pepstatin is a slow and tight-binding inhibitor of pepsin. Preincubating enzyme and inhibitor in H2O and in D2O in the absence of substrate generates an inverse solvent isotope effect of Dk = 0.69 +/- 0.06 on the apparent first-order rate constant for the decay in enzymatic activity. Proton inventory analysis of the i...

journal_title：Biochemistry

authors： Cho YK,Rebholz KL,Northrop DB

更新日期：1994-08-16 00:00:00

abstract：:Muscle fiber contraction is regulated through calcium-induced changes in the conformation of troponin C. In this study, we explored the relationship between the stability of a specific helix in the protein and the metal ion affinity of associated binding sites. Serial replacement of the amino acid at position 130 caus...

journal_title：Biochemistry

authors： Trigo-Gonzalez G,Awang G,Racher K,Neden K,Borgford T

更新日期：1993-09-21 00:00:00

abstract：:Bacterial resistance to the aminoglycoside antibiotics is manifested primarily through the production of enzymes which covalently modify these drugs. The Enterococci and Staphylococci produce an ATP-dependent kinase, APH(3')-IIIa, which phosphorylates such antibiotics as kanamycin, amikacin, and neomycin, and this enz...

journal_title：Biochemistry

authors： McKay GA,Wright GD

更新日期：1996-07-02 00:00:00

abstract：:Soluble inorganic pyrophosphatase (PPase) is one of the better understood phosphoryl-transfer enzymes and is distinctive in having four divalent metal ions at the active site. Here we determine pH profiles for wild-type Saccharomyces cerevisiae PPase (Y-PPase) and for 14 of its active site variants and consider the ef...

journal_title：Biochemistry

authors： Pohjanjoki P,Lahti R,Goldman A,Cooperman BS

更新日期：1998-02-17 00:00:00

abstract：:Apolipoproteins A-I and A-II form the major protein constituents of high-density lipid particles (HDL), the concentration of which is inversely correlated with the frequency of heart disease in humans. Although the physiological role of apolipoprotein A-II is unclear, evidence for its involvement in free fatty acid me...

journal_title：Biochemistry

authors： Kumar MS,Carson M,Hussain MM,Murthy HM

更新日期：2002-10-01 00:00:00

abstract：:Semisynthetic aminoglycoside derivatives may provide a means to selectively target viral RNA sites, including the HIV-1 Rev response element (RRE). The design, synthesis, and evaluation of derivatives based upon neomycin B, kanamycin A, and tobramycin conjugates of 9-aminoacridine are presented. To evaluate the import...

journal_title：Biochemistry

authors： Luedtke NW,Liu Q,Tor Y

更新日期：2003-10-07 00:00:00

abstract：:The size and surface curvature dependence of the properties and stability of single lamellar vesicles have been investigated by using a variety of physicochemical techniques. Dipalmitoylphosphatidylcholine single lamellar vesicles of sizes ranging between 200 and 900 A in diameter have been prepared by the French pres...

journal_title：Biochemistry

authors： Lichtenberg D,Freire E,Schmidt CF,Barenholz Y,Felgner PL,Thompson TE

更新日期：1981-06-09 00:00:00

abstract：:Unfractionated Escherichia coli tRNA has been aminoacylated with lysine and preferentially acetylated at the epsilon-amino nitrogen of lysine by reaction with N-acetoxysuccinimide. After treatment with peptidyl-tRNA hydrolase, 90% of the aminoacylated tRNA molecules were Nepsilon-acetyl-Lys-tRNA. Post-ribosomal supern...

journal_title：Biochemistry

authors： Johnson AE,Woodward WR,Herbert E,Menninger JR

更新日期：1976-02-10 00:00:00

abstract：:Chemical-modification studies on submitochondrial particle pyridine dinucleotide transhydrogenase (EC 1.6.1.1) demonstrate the presence of one class of sulfhydryl group in the nicotinamide adenine dinucleotide phosphate (NADP) site and another peripheral to the active site. Reaction of the peripheral sulfhydryl group ...

journal_title：Biochemistry

authors： Earle SR,O'Neal SG,Fisher RR

更新日期：1978-10-31 00:00:00

abstract：:Active trihydroxynaphthalene reductase (3HNR) is essential for the biosynthesis of fungal melanin by Magnaporthe grisea and is a focus of inhibitor design studies directed toward control of blast disease in rice. Tricyclazole, a preventative fungicide against rice blast, has been previously characterized as inhibiting...

journal_title：Biochemistry

authors： Thompson JE,Basarab GS,Andersson A,Lindqvist Y,Jordan DB

更新日期：1997-02-18 00:00:00

abstract：:Simple procedures for the anaerobic preparation of photoactive and stable P840 reaction centers from Chlorobium tepidum and Chlorobium limicola in good yield are presented and quantitated. The subunit composition was tested by cosedimentation in sucrose density gradients. For C. limicola, it minimally comprises four s...

journal_title：Biochemistry

authors： Hager-Braun C,Xie DL,Jarosch U,Herold E,Büttner M,Zimmermann R,Deutzmann R,Hauska G,Nelson N

更新日期：1995-07-25 00:00:00

abstract：:Mammalian cGMP- and cAMP-dependent protein kinase show considerable similarity in amino acid sequence, although they specifically bind different cyclic nucleotides. Results of cGMP analogue binding experiments, combined with modeling of the cGMP binding sites by analogy to the structure of the homologous catabolite ge...

journal_title：Biochemistry

authors： Weber IT,Shabb JB,Corbin JD

更新日期：1989-07-11 00:00:00

abstract：:The growth and shortening of microtubules in guanosine triphosphate-(GTP-) mediated dynamic instability has previously been observed to occur at rates which are remarkably variable (Gildersleeve et al., 1992, Chrétien et al., 1995). Neighboring microtubules observed simultaneously can grow or shorten at different rate...

journal_title：Biochemistry

authors： Dye RB,Williams RC Jr

更新日期：1996-11-12 00:00:00

abstract：:The interaction of nile red (NR) with apomyoglobin (ApoMb) in the native (pH 7) and molten globule (pH 4) states was investigated using experimental and computational methods. NR binds to hydrophobic locations in ApoMb with higher affinity (K(d) = 25 +/- 5 microM) in the native state than in the molten globule state (...

journal_title：Biochemistry

authors： Polverini E,Cugini G,Annoni F,Abbruzzetti S,Viappiani C,Gensch T

更新日期：2006-04-25 00:00:00

abstract：:The metalloregulatory protein MntR from Bacillus subtilis acts as a transcriptional regulator of manganese homeostasis. MntR is a member of a subfamily of DtxR-related proteins that perform analogous regulatory functions in a variety of pathogenic organisms. Metal ions activate MntR to bind DNA and repress the transcr...

journal_title：Biochemistry

authors： Lieser SA,Davis TC,Helmann JD,Cohen SM

更新日期：2003-11-04 00:00:00

abstract：:We use laser flash photolysis and time-resolved Raman spectroscopy of CO-bound H93G myoglobin (Mb) mutants to study the influence of the proximal ligand on the CO rebinding kinetics. In H93G mutants, where the proximal linkage with the protein is eliminated and the heme can bind exogenous ligands (e.g., imidazole, 4-b...

journal_title：Biochemistry

authors： Cao W,Ye X,Sjodin T,Christian JF,Demidov AA,Berezhna S,Wang W,Barrick D,Sage JT,Champion PM

更新日期：2004-08-31 00:00:00

abstract：:Alkalimetric, spectrophotometric, NMR, and resonance Raman titrations are reported for the sulfonamide Neoprontosil in aqueous solution. An assignment of the magnetic resonance peaks for each of the Neoprontosil protons has been made. Neoprontosil is shown to have two "coupled" iity of the microscopic pKs for these tw...

journal_title：Biochemistry

authors： Carey PR,King RW

更新日期：1979-06-26 00:00:00

abstract：:X-ray absorption spectra are reported for the multi-Cu oxidase Rhus vernicifera laccase in oxidized and fully reduced forms and for laccase from which the type 2 Cu has been depleted (T2D). The structure of the Cu K edge for both preparations shows the presence of CuII and CuI in the oxidized and reduced states, respe...

journal_title：Biochemistry

authors： Woolery GL,Powers L,Peisach J,Spiro TG

更新日期：1984-07-17 00:00:00

abstract：:Membrane proteins are embedded in a lipid bilayer and maintain strong interactions with lipid molecules. Tightly bound lipids are responsible for vertical positioning and integration of proteins in the membrane and for assembly of multisubunit complexes and occasionally act as substrates. In this work we present the l...

journal_title：Biochemistry

authors： Wöhri AB,Wahlgren WY,Malmerberg E,Johansson LC,Neutze R,Katona G

更新日期：2009-10-20 00:00:00

abstract：:The biochemical characteristics of the formation and disappearance of intercalator-induced DNA double-strand breaks (DSB) were studied in nuclei from mouse leukemia L1210 cells by using filter elution methodology [Bradley, M. O., & Kohn, K.W. (1979) Nucleic Acids Res. 7, 793-804]. The three intercalators used were 4'-...

journal_title：Biochemistry

authors： Pommier Y,Schwartz RE,Kohn KW,Zwelling LA

更新日期：1984-07-03 00:00:00

abstract：:The expression of rusticyanin in Escherichia coli and a number of mutants for Ser86 is reported. Mutations of Ser86 to Asn, Asp, Gln, and Leu were undertaken as this is an Asn residue in other structurally characterized cupredoxins, and it has been suggested that this may be partly responsible for the high redox poten...

journal_title：Biochemistry

authors： Hall JF,Kanbi LD,Harvey I,Murphy LM,Hasnain SS

更新日期：1998-08-18 00:00:00

abstract：:Nucleolin, also called protein C23, is a RNA-associated protein implicated in the early stages of ribosome assembly. To study the general conformation and map the nucleic acid binding regions, rat nucleolin was subjected to limited proteolysis using trypsin and chymotrypsin in the presence or absence of poly(G). The c...

journal_title：Biochemistry

authors： Olson MO,Kirstein MN,Wallace MO

更新日期：1990-06-19 00:00:00

abstract：:The non-typeable Haemophilus influenzae strain DH1 was isolated from a 25 year old male patient with Fisher syndrome, a postinfectious autoimmune condition characterized by the presence of anti-GQ1b IgG antibodies that target and initiate damage to peripheral nerves. DH1 was found to display an alphaNeuAc(2-8)alphaNeu...

journal_title：Biochemistry

authors： Houliston RS,Koga M,Li J,Jarrell HC,Richards JC,Vitiazeva V,Schweda EK,Yuki N,Gilbert M

更新日期：2007-07-10 00:00:00

abstract：:When the single-stranded RNA genome of HIV-1 is copied into double-stranded DNA, the viral enzyme reverse transcriptase (RT) catalyzes the addition of approximately 20,000 nucleotides; however, the precise mechanism of nucleotide addition is unknown. In this study, we attempt to integrate the genetic data and biochemi...

journal_title：Biochemistry

authors： Patel PH,Jacobo-Molina A,Ding J,Tantillo C,Clark AD Jr,Raag R,Nanni RG,Hughes SH,Arnold E

更新日期：1995-04-25 00:00:00

abstract：:Conformational distributions of a four-way DNA junction have been examined by time-resolved fluorescence resonance energy transfer (FRET). A series of dye-labeled junctions were synthesized with donor (fluorescein) and acceptor (tetramethylrhodamine) dyes conjugated to the 5' termini of the duplex arms in all six pair...

journal_title：Biochemistry

authors： Eis PS,Millar DP

更新日期：1993-12-21 00:00:00

abstract：:The only identified component of the scrapie prion is PrPSc, a glycosylinositol phospholipid (GPI)-linked protein that is derived from the cellular isoform (PrPC) by an as yet unknown posttranslational event. Analysis of the PrPSc GPI has revealed six different glycoforms, three of which are unprecedented. Two of the ...

journal_title：Biochemistry

authors： Stahl N,Baldwin MA,Hecker R,Pan KM,Burlingame AL,Prusiner SB

更新日期：1992-06-02 00:00:00

abstract：:Glycophorin A is a sialoglycoprotein isolated from human erythrocyte membranes which seems to exist as stable dimeric complexes in the presence of sodium dodecyl sulfate. When analyzed by dodecyl sulfate acrylamide electrophoresis this molecule forms two PAS-stainable bands (PAS-U and PAS-2) which are reversibly inter...

journal_title：Biochemistry

authors： Furthmayr H,Marchesi VT

更新日期：1976-03-09 00:00:00

abstract：:Thirty single cysteine substitution mutants of T4 lysozyme have been prepared and spin-labeled with a sulfhydryl-specific nitroxide reagent in order to systematically investigate the relationship between nitroxide side-chain mobility and protein structure. The perturbation caused by replacement of a native residue wit...

journal_title：Biochemistry

authors： Mchaourab HS,Lietzow MA,Hideg K,Hubbell WL

更新日期：1996-06-18 00:00:00

abstract：:Transferrin exhibits heterogeneity in glycosylation characteristic of pathological changes in alcohol abuse and congenital disorders in glycosylation. This study investigated an alternative approach in the detection of carbohydrate-deficient transferrin based on the premise that glycosylation may afford some degree of...

journal_title：Biochemistry

authors： Valmu L,Kalkkinen N,Husa A,Rye PD

更新日期：2005-12-13 00:00:00